Esterase (gene Lip2) from Monascus purpureus M7

Enzyme Description

Species
Monascus purpureus M7 β†—
Extremophile
No
EC Number
3.1.1.1 β†—

Sequence

Length: 258 amino acids
MSSIEEGTSASGAQGLLYQPYDQFMLFGDSITEFSCNQSMGFAFHPALQNSYIRRLDVINRGFSGYTSANALQVLPSFFPTPRTAKVRLMTIFFGANDACLPGKFQHVPLGEYKKNLEKLIEHPAVKEQNPRIIIITPPPVNEYQFFDMDDPSRIASNTKLYAEAAREVAGSLGVPVADIWTRFMEAAGWQEGQPLLGSRDVPESEKLQSFLTDGLHLTPDGYRIVYEEVMEVIRVNWPDQTPEQLSRVFPGWQDAPK
Li-Jing Kang et al. (2017) β€” Screening, purification, and characterization of a novel organic solvent-tolerant esterase, Lip2, from Monascus purpureus strain M7
Extremophiles  Β· doi:10.1007/s00792-016-0907-x β†—  Β· Activity - Classical Stability - Incubation
48 measurements ▼
Database ID
UniProt: A0A1B2CT54 β†—
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (48 measurements)

48 measurements β€” page 1 of 3
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent Ethanol 20% 100 117 % 50 mM Tris-HCl buffer, 2% acetonitrile 8 40Β°C 0.8 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” 160 rpm Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent Methanol 20% 100 143 % 50 mM Tris-HCl buffer, 2% acetonitrile 8 40Β°C 0.8 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” 160 rpm Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent Chloroform 20% 100 101 % 50 mM Tris-HCl buffer, 2% acetonitrile 8 40Β°C 0.8 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” 160 rpm Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent n-Heptane 20% 100 108 % 50 mM Tris-HCl buffer, 2% acetonitrile 8 40Β°C 0.8 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” 160 rpm Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent n-Hexane 20% 100 129 % 50 mM Tris-HCl buffer, 2% acetonitrile 8 40Β°C 0.8 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” 160 rpm Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent Cyclohexane 20% 100 90 % 50 mM Tris-HCl buffer, 2% acetonitrile 8 40Β°C 0.8 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” 160 rpm Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent Toluene 20% 100 125 % 50 mM Tris-HCl buffer, 2% acetonitrile 8 40Β°C 0.8 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” 160 rpm Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent n-Hexane 20% 100 101 % 50 mM Tris-HCl buffer, 2% acetonitrile 8 40Β°C 0.8 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” 160 rpm Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent Acetone 20% 100 99 % 50 mM Tris-HCl buffer, 2% acetonitrile 8 40Β°C 0.8 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” 160 rpm Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent Glycerol 20% 100 107 % 50 mM Tris-HCl buffer, 2% acetonitrile 8 40Β°C 0.8 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” 160 rpm Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 20% 100 95 % 50 mM Tris-HCl buffer, 2% acetonitrile 8 40Β°C 0.8 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” 160 rpm Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent Acetonitrile 20% 100 166 % 50 mM Tris-HCl buffer, 2% acetonitrile 8 40Β°C 0.8 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” 160 rpm Classical aqueous control (in %)
Stability - Incubation Activity after incubation (3 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase n-Hexane 20% 100 143 % Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 2% acetonitrile Incubation: 8, Assay: 8 Incubation: 40Β°C, Assay: 40Β°C 0.8 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 160 rpm, Assay: 160 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (24 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase Chloroform 20% 100 100 % Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 2% acetonitrile Incubation: 8, Assay: 8 Incubation: 40Β°C, Assay: 40Β°C 0.8 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 160 rpm, Assay: 160 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (3 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase Toluene 20% 100 160 % Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 2% acetonitrile Incubation: 8, Assay: 8 Incubation: 40Β°C, Assay: 40Β°C 0.8 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 160 rpm, Assay: 160 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (3 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase Cyclohexane 20% 100 114 % Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 2% acetonitrile Incubation: 8, Assay: 8 Incubation: 40Β°C, Assay: 40Β°C 0.8 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 160 rpm, Assay: 160 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (3 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase n-Hexane 20% 100 158 % Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 2% acetonitrile Incubation: 8, Assay: 8 Incubation: 40Β°C, Assay: 40Β°C 0.8 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 160 rpm, Assay: 160 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (3 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase n-Heptane 20% 100 137 % Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 2% acetonitrile Incubation: 8, Assay: 8 Incubation: 40Β°C, Assay: 40Β°C 0.8 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 160 rpm, Assay: 160 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (3 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase Chloroform 20% 100 122 % Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 2% acetonitrile Incubation: 8, Assay: 8 Incubation: 40Β°C, Assay: 40Β°C 0.8 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 160 rpm, Assay: 160 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (24 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase Methanol 20% 100 110 % Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 2% acetonitrile Incubation: 8, Assay: 8 Incubation: 40Β°C, Assay: 40Β°C 0.8 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 160 rpm, Assay: 160 rpm Non-incubated control (in %), assay in aqueous phase
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Visualization : Activity β€” Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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