Esterase (gene Lip2) from Monascus purpureus M7

Enzyme Description

Species
Monascus purpureus M7 β†—
Extremophile
No
EC Number
3.1.1.1 β†—

Sequence

Length: 258 amino acids
MSSIEEGTSASGAQGLLYQPYDQFMLFGDSITEFSCNQSMGFAFHPALQNSYIRRLDVINRGFSGYTSANALQVLPSFFPTPRTAKVRLMTIFFGANDACLPGKFQHVPLGEYKKNLEKLIEHPAVKEQNPRIIIITPPPVNEYQFFDMDDPSRIASNTKLYAEAAREVAGSLGVPVADIWTRFMEAAGWQEGQPLLGSRDVPESEKLQSFLTDGLHLTPDGYRIVYEEVMEVIRVNWPDQTPEQLSRVFPGWQDAPK
Li-Jing Kang et al. (2017) β€” Screening, purification, and characterization of a novel organic solvent-tolerant esterase, Lip2, from Monascus purpureus strain M7
Extremophiles  Β· doi:10.1007/s00792-016-0907-x β†—  Β· Activity - Classical Stability - Incubation
48 measurements ▼
Database ID
UniProt: A0A1B2CT54 β†—
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (48 measurements)

48 measurements β€” page 3 of 3
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (0.5 hour at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase n-Hexane 20% 100 134 % Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 2% acetonitrile Incubation: 8, Assay: 8 Incubation: 40Β°C, Assay: 40Β°C 0.8 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 160 rpm, Assay: 160 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (0.5 hour at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase n-Heptane 20% 100 112 % Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 2% acetonitrile Incubation: 8, Assay: 8 Incubation: 40Β°C, Assay: 40Β°C 0.8 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 160 rpm, Assay: 160 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (0.5 hour at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase Chloroform 20% 100 107 % Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 2% acetonitrile Incubation: 8, Assay: 8 Incubation: 40Β°C, Assay: 40Β°C 0.8 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 160 rpm, Assay: 160 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (3 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase Methanol 20% 100 190 % Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 2% acetonitrile Incubation: 8, Assay: 8 Incubation: 40Β°C, Assay: 40Β°C 0.8 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 160 rpm, Assay: 160 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (3 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase Ethanol 20% 100 182 % Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 2% acetonitrile Incubation: 8, Assay: 8 Incubation: 40Β°C, Assay: 40Β°C 0.8 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 160 rpm, Assay: 160 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (3 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase Acetonitrile 20% 100 129 % Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 2% acetonitrile Incubation: 8, Assay: 8 Incubation: 40Β°C, Assay: 40Β°C 0.8 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 160 rpm, Assay: 160 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (3 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 20% 100 104 % Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 2% acetonitrile Incubation: 8, Assay: 8 Incubation: 40Β°C, Assay: 40Β°C 0.8 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 160 rpm, Assay: 160 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (3 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase Glycerol 20% 100 131 % Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 2% acetonitrile Incubation: 8, Assay: 8 Incubation: 40Β°C, Assay: 40Β°C 0.8 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 160 rpm, Assay: 160 rpm Non-incubated control (in %), assay in aqueous phase
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Visualization : Activity β€” Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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