Esterase (gene Lip2) from Monascus purpureus M7
Enzyme Description
Sequence
MSSIEEGTSASGAQGLLYQPYDQFMLFGDSITEFSCNQSMGFAFHPALQNSYIRRLDVINRGFSGYTSANALQVLPSFFPTPRTAKVRLMTIFFGANDACLPGKFQHVPLGEYKKNLEKLIEHPAVKEQNPRIIIITPPPVNEYQFFDMDDPSRIASNTKLYAEAAREVAGSLGVPVADIWTRFMEAAGWQEGQPLLGSRDVPESEKLQSFLTDGLHLTPDGYRIVYEEVMEVIRVNWPDQTPEQLSRVFPGWQDAPK
Li-Jing Kang et al. (2017)
β Screening, purification, and characterization of a novel organic solvent-tolerant esterase, Lip2, from Monascus purpureus strain M7
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Database ID
UniProt: A0A1B2CT54 β
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)
Experimental Data (48 measurements)
48 measurements β page 2 of 3
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Stability - Incubation | Activity after incubation (24 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase | Ethanol | 20% | 100 | 107 | % | Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 2% acetonitrile | Incubation: 8, Assay: 8 | Incubation: 40Β°C, Assay: 40Β°C | 0.8 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | Incubation: 160 rpm, Assay: 160 rpm | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (24 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase | Acetonitrile | 20% | 100 | 99 | % | Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 2% acetonitrile | Incubation: 8, Assay: 8 | Incubation: 40Β°C, Assay: 40Β°C | 0.8 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | Incubation: 160 rpm, Assay: 160 rpm | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (24 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 20% | 100 | 107 | % | Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 2% acetonitrile | Incubation: 8, Assay: 8 | Incubation: 40Β°C, Assay: 40Β°C | 0.8 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | Incubation: 160 rpm, Assay: 160 rpm | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (24 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase | Glycerol | 20% | 100 | 109 | % | Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 2% acetonitrile | Incubation: 8, Assay: 8 | Incubation: 40Β°C, Assay: 40Β°C | 0.8 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | Incubation: 160 rpm, Assay: 160 rpm | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (24 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase | Acetone | 20% | 100 | 102 | % | Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 2% acetonitrile | Incubation: 8, Assay: 8 | Incubation: 40Β°C, Assay: 40Β°C | 0.8 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | Incubation: 160 rpm, Assay: 160 rpm | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (24 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase | n-Hexane | 20% | 100 | 98 | % | Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 2% acetonitrile | Incubation: 8, Assay: 8 | Incubation: 40Β°C, Assay: 40Β°C | 0.8 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | Incubation: 160 rpm, Assay: 160 rpm | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (24 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase | Toluene | 20% | 100 | 89 | % | Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 2% acetonitrile | Incubation: 8, Assay: 8 | Incubation: 40Β°C, Assay: 40Β°C | 0.8 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | Incubation: 160 rpm, Assay: 160 rpm | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (24 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase | Cyclohexane | 20% | 100 | 72 | % | Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 2% acetonitrile | Incubation: 8, Assay: 8 | Incubation: 40Β°C, Assay: 40Β°C | 0.8 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | Incubation: 160 rpm, Assay: 160 rpm | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (24 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase | n-Hexane | 20% | 100 | 71 | % | Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 2% acetonitrile | Incubation: 8, Assay: 8 | Incubation: 40Β°C, Assay: 40Β°C | 0.8 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | Incubation: 160 rpm, Assay: 160 rpm | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (24 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase | n-Heptane | 20% | 100 | 102 | % | Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 2% acetonitrile | Incubation: 8, Assay: 8 | Incubation: 40Β°C, Assay: 40Β°C | 0.8 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | Incubation: 160 rpm, Assay: 160 rpm | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (0.5 hour at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase | Cyclohexane | 20% | 100 | 98 | % | Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 2% acetonitrile | Incubation: 8, Assay: 8 | Incubation: 40Β°C, Assay: 40Β°C | 0.8 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | Incubation: 160 rpm, Assay: 160 rpm | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (0.5 hour at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase | Methanol | 20% | 100 | 115 | % | Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 2% acetonitrile | Incubation: 8, Assay: 8 | Incubation: 40Β°C, Assay: 40Β°C | 0.8 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | Incubation: 160 rpm, Assay: 160 rpm | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (0.5 hour at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase | Ethanol | 20% | 100 | 124 | % | Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 2% acetonitrile | Incubation: 8, Assay: 8 | Incubation: 40Β°C, Assay: 40Β°C | 0.8 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | Incubation: 160 rpm, Assay: 160 rpm | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (0.5 hour at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase | Acetonitrile | 20% | 100 | 147 | % | Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 2% acetonitrile | Incubation: 8, Assay: 8 | Incubation: 40Β°C, Assay: 40Β°C | 0.8 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | Incubation: 160 rpm, Assay: 160 rpm | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (0.5 hour at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 20% | 100 | 96 | % | Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 2% acetonitrile | Incubation: 8, Assay: 8 | Incubation: 40Β°C, Assay: 40Β°C | 0.8 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | Incubation: 160 rpm, Assay: 160 rpm | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (0.5 hour at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase | Glycerol | 20% | 100 | 105 | % | Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 2% acetonitrile | Incubation: 8, Assay: 8 | Incubation: 40Β°C, Assay: 40Β°C | 0.8 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | Incubation: 160 rpm, Assay: 160 rpm | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (0.5 hour at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase | Acetone | 20% | 100 | 101 | % | Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 2% acetonitrile | Incubation: 8, Assay: 8 | Incubation: 40Β°C, Assay: 40Β°C | 0.8 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | Incubation: 160 rpm, Assay: 160 rpm | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (0.5 hour at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase | n-Hexane | 20% | 100 | 104 | % | Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 2% acetonitrile | Incubation: 8, Assay: 8 | Incubation: 40Β°C, Assay: 40Β°C | 0.8 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | Incubation: 160 rpm, Assay: 160 rpm | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (0.5 hour at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase | Toluene | 20% | 100 | 132 | % | Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 2% acetonitrile | Incubation: 8, Assay: 8 | Incubation: 40Β°C, Assay: 40Β°C | 0.8 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | Incubation: 160 rpm, Assay: 160 rpm | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (3 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase | Acetone | 20% | 100 | 126 | % | Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 2% acetonitrile | Incubation: 8, Assay: 8 | Incubation: 40Β°C, Assay: 40Β°C | 0.8 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | Incubation: 160 rpm, Assay: 160 rpm | Non-incubated control (in %), assay in aqueous phase |
Visualization : Activity β Classical
One bar per measurement. Colour = solvent, shade = solvent volume.
Visualization : Stability β Incubation
One bar per measurement. Colour = solvent, shade = solvent volume.