Cytochrome P450 1A2 from Homo sapiens
Enzyme Description
Sequence
MALSQSVPFSATELLLASAIFCLVFWVLKGLRPRVPKGLKSPPEPWGWPLLGHVLTLGKNPHLALSRMSQRYGDVLQIRIGSTPVLVLSRLDTIRQALVRQGDDFKGRPDLYTSTLITDGQSLTFSTDSGPVWAARRRLAQNALNTFSIASDPASSSSCYLEEHVSKEAKALISRLQELMAGPGHFDPYNQVVVSVANVIGAMCFGQHFPESSDEMLSLVKNTHEFVETASSGNPLDFFPILRYLPNPALQRFKAFNQRFLWFLQKTVQEHYQDFDKNSVRDITGALFKHSKKGPRASGNLIPQEKIVNLVNDIFGAGFDTVTTAISWSLMYLVTKPEIQRKIQKELDTVIGRERRPRLSDRPQLPYLEAFILETFRHSSFLPFTIPHSTTRDTTLNGFYIPKKCCVFVNQWQVNHDPELWEDPSEFRPERFLTADGTAINKPLSEKMMLFGMGKRRCIGEVLAKWEIFLFLAILLQQLEFSVPPGVKVDLTPIYGLTMKHARCEHVQARLRFSIN
Dennis Rokitta et al. (2013)
β The effect of organic solvents on enzyme kinetic parameters of human CYP3A4 and CYP1A2 in vitro
Toxicology Mechanisms and Methods
Β· doi:10.3109/15376516.2013.806622 β
Β· Activity - Michaelis-Menten
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Database ID
UniProt: P05177 β
Sequence Annotation
Inferred - from protein name
Protein Source
Commercially purchased
Experimental Data (48 measurements)
48 measurements β page 1 of 3
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Activity - Michaelis-Menten | Vmax/kM (in nmol/min/ΞΌM/nmol CYP) measured by LC/MS after incubation (20 minutes at 37Β°C) in the presence of organic solvent | Methanol | 1% | 0.16 | 0.16 | nmol/min/ΞΌM/nmol CYP | 100 mM potassium phosphate buffer | 7.4 | 37Β°C | Caffeine | Paraxanthine | NADPH regenerating system (containing NADP+, glucose-6-phosphate and glucose-6-phosphate-dehydrogenase), no clear concentration | Yes, "shaking water bath" | Classical aqueous control (in nmol/min/ΞΌM/nmol CYP) | Enzymes in supersomes |
| Activity - Michaelis-Menten | Vmax (in nmol/min/nmol CYP) measured by LC/MS after incubation (20 minutes at 37Β°C) in the presence of organic solvent | Ethanol | 2% | 172.0 | 72.0 | nmol/min/nmol CYP | 100 mM potassium phosphate buffer | 7.4 | 37Β°C | Caffeine | Paraxanthine | NADPH regenerating system (containing NADP+, glucose-6-phosphate and glucose-6-phosphate-dehydrogenase), no clear concentration | Yes, "shaking water bath" | Classical aqueous control (in nmol/min/nmol CYP) | Enzymes in supersomes |
| Activity - Michaelis-Menten | Vmax (in nmol/min/nmol CYP) measured by LC/MS after incubation (20 minutes at 37Β°C) in the presence of organic solvent | Ethanol | 3% | 172.0 | 68.0 | nmol/min/nmol CYP | 100 mM potassium phosphate buffer | 7.4 | 37Β°C | Caffeine | Paraxanthine | NADPH regenerating system (containing NADP+, glucose-6-phosphate and glucose-6-phosphate-dehydrogenase), no clear concentration | Yes, "shaking water bath" | Classical aqueous control (in nmol/min/nmol CYP) | Enzymes in supersomes |
| Activity - Michaelis-Menten | Vmax (in nmol/min/nmol CYP) measured by LC/MS after incubation (20 minutes at 37Β°C) in the presence of organic solvent | Ethanol | 4% | 172.0 | 51.0 | nmol/min/nmol CYP | 100 mM potassium phosphate buffer | 7.4 | 37Β°C | Caffeine | Paraxanthine | NADPH regenerating system (containing NADP+, glucose-6-phosphate and glucose-6-phosphate-dehydrogenase), no clear concentration | Yes, "shaking water bath" | Classical aqueous control (in nmol/min/nmol CYP) | Enzymes in supersomes |
| Activity - Michaelis-Menten | Vmax (in nmol/min/nmol CYP) measured by LC/MS after incubation (20 minutes at 37Β°C) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 1% | 172.0 | 135.0 | nmol/min/nmol CYP | 100 mM potassium phosphate buffer | 7.4 | 37Β°C | Caffeine | Paraxanthine | NADPH regenerating system (containing NADP+, glucose-6-phosphate and glucose-6-phosphate-dehydrogenase), no clear concentration | Yes, "shaking water bath" | Classical aqueous control (in nmol/min/nmol CYP) | Enzymes in supersomes |
| Activity - Michaelis-Menten | Vmax (in nmol/min/nmol CYP) measured by LC/MS after incubation (20 minutes at 37Β°C) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 2% | 172.0 | 101.0 | nmol/min/nmol CYP | 100 mM potassium phosphate buffer | 7.4 | 37Β°C | Caffeine | Paraxanthine | NADPH regenerating system (containing NADP+, glucose-6-phosphate and glucose-6-phosphate-dehydrogenase), no clear concentration | Yes, "shaking water bath" | Classical aqueous control (in nmol/min/nmol CYP) | Enzymes in supersomes |
| Activity - Michaelis-Menten | Vmax (in nmol/min/nmol CYP) measured by LC/MS after incubation (20 minutes at 37Β°C) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 3% | 172.0 | 149.0 | nmol/min/nmol CYP | 100 mM potassium phosphate buffer | 7.4 | 37Β°C | Caffeine | Paraxanthine | NADPH regenerating system (containing NADP+, glucose-6-phosphate and glucose-6-phosphate-dehydrogenase), no clear concentration | Yes, "shaking water bath" | Classical aqueous control (in nmol/min/nmol CYP) | Enzymes in supersomes |
| Activity - Michaelis-Menten | Vmax (in nmol/min/nmol CYP) measured by LC/MS after incubation (20 minutes at 37Β°C) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 4% | 172.0 | 71.0 | nmol/min/nmol CYP | 100 mM potassium phosphate buffer | 7.4 | 37Β°C | Caffeine | Paraxanthine | NADPH regenerating system (containing NADP+, glucose-6-phosphate and glucose-6-phosphate-dehydrogenase), no clear concentration | Yes, "shaking water bath" | Classical aqueous control (in nmol/min/nmol CYP) | Enzymes in supersomes |
| Activity - Michaelis-Menten | Vmax/kM (in nmol/min/ΞΌM/nmol CYP) measured by LC/MS after incubation (20 minutes at 37Β°C) in the presence of organic solvent | Acetonitrile | 1% | 0.16 | 1.9 | nmol/min/ΞΌM/nmol CYP | 100 mM potassium phosphate buffer | 7.4 | 37Β°C | Caffeine | Paraxanthine | NADPH regenerating system (containing NADP+, glucose-6-phosphate and glucose-6-phosphate-dehydrogenase), no clear concentration | Yes, "shaking water bath" | Classical aqueous control (in nmol/min/ΞΌM/nmol CYP) | Enzymes in supersomes |
| Activity - Michaelis-Menten | Vmax/kM (in nmol/min/ΞΌM/nmol CYP) measured by LC/MS after incubation (20 minutes at 37Β°C) in the presence of organic solvent | Acetonitrile | 2% | 0.16 | 2.53 | nmol/min/ΞΌM/nmol CYP | 100 mM potassium phosphate buffer | 7.4 | 37Β°C | Caffeine | Paraxanthine | NADPH regenerating system (containing NADP+, glucose-6-phosphate and glucose-6-phosphate-dehydrogenase), no clear concentration | Yes, "shaking water bath" | Classical aqueous control (in nmol/min/ΞΌM/nmol CYP) | Enzymes in supersomes |
| Activity - Michaelis-Menten | Vmax/kM (in nmol/min/ΞΌM/nmol CYP) measured by LC/MS after incubation (20 minutes at 37Β°C) in the presence of organic solvent | Acetonitrile | 3% | 0.16 | 1.91 | nmol/min/ΞΌM/nmol CYP | 100 mM potassium phosphate buffer | 7.4 | 37Β°C | Caffeine | Paraxanthine | NADPH regenerating system (containing NADP+, glucose-6-phosphate and glucose-6-phosphate-dehydrogenase), no clear concentration | Yes, "shaking water bath" | Classical aqueous control (in nmol/min/ΞΌM/nmol CYP) | Enzymes in supersomes |
| Activity - Michaelis-Menten | Vmax/kM (in nmol/min/ΞΌM/nmol CYP) measured by LC/MS after incubation (20 minutes at 37Β°C) in the presence of organic solvent | Acetonitrile | 4% | 0.16 | 1.06 | nmol/min/ΞΌM/nmol CYP | 100 mM potassium phosphate buffer | 7.4 | 37Β°C | Caffeine | Paraxanthine | NADPH regenerating system (containing NADP+, glucose-6-phosphate and glucose-6-phosphate-dehydrogenase), no clear concentration | Yes, "shaking water bath" | Classical aqueous control (in nmol/min/ΞΌM/nmol CYP) | Enzymes in supersomes |
| Activity - Michaelis-Menten | Vmax (in nmol/min/nmol CYP) measured by LC/MS after incubation (20 minutes at 37Β°C) in the presence of organic solvent | Ethanol | 1% | 172.0 | 66.0 | nmol/min/nmol CYP | 100 mM potassium phosphate buffer | 7.4 | 37Β°C | Caffeine | Paraxanthine | NADPH regenerating system (containing NADP+, glucose-6-phosphate and glucose-6-phosphate-dehydrogenase), no clear concentration | Yes, "shaking water bath" | Classical aqueous control (in nmol/min/nmol CYP) | Enzymes in supersomes |
| Activity - Michaelis-Menten | Vmax/kM (in nmol/min/ΞΌM/nmol CYP) measured by LC/MS after incubation (20 minutes at 37Β°C) in the presence of organic solvent | Methanol | 2% | 0.16 | 0.2 | nmol/min/ΞΌM/nmol CYP | 100 mM potassium phosphate buffer | 7.4 | 37Β°C | Caffeine | Paraxanthine | NADPH regenerating system (containing NADP+, glucose-6-phosphate and glucose-6-phosphate-dehydrogenase), no clear concentration | Yes, "shaking water bath" | Classical aqueous control (in nmol/min/ΞΌM/nmol CYP) | Enzymes in supersomes |
| Activity - Michaelis-Menten | Vmax/kM (in nmol/min/ΞΌM/nmol CYP) measured by LC/MS after incubation (20 minutes at 37Β°C) in the presence of organic solvent | Methanol | 3% | 0.16 | 0.2 | nmol/min/ΞΌM/nmol CYP | 100 mM potassium phosphate buffer | 7.4 | 37Β°C | Caffeine | Paraxanthine | NADPH regenerating system (containing NADP+, glucose-6-phosphate and glucose-6-phosphate-dehydrogenase), no clear concentration | Yes, "shaking water bath" | Classical aqueous control (in nmol/min/ΞΌM/nmol CYP) | Enzymes in supersomes |
| Activity - Michaelis-Menten | Vmax/kM (in nmol/min/ΞΌM/nmol CYP) measured by LC/MS after incubation (20 minutes at 37Β°C) in the presence of organic solvent | Methanol | 4% | 0.16 | 0.19 | nmol/min/ΞΌM/nmol CYP | 100 mM potassium phosphate buffer | 7.4 | 37Β°C | Caffeine | Paraxanthine | NADPH regenerating system (containing NADP+, glucose-6-phosphate and glucose-6-phosphate-dehydrogenase), no clear concentration | Yes, "shaking water bath" | Classical aqueous control (in nmol/min/ΞΌM/nmol CYP) | Enzymes in supersomes |
| Activity - Michaelis-Menten | Vmax/kM (in nmol/min/ΞΌM/nmol CYP) measured by LC/MS after incubation (20 minutes at 37Β°C) in the presence of organic solvent | Ethanol | 1% | 0.16 | 0.43 | nmol/min/ΞΌM/nmol CYP | 100 mM potassium phosphate buffer | 7.4 | 37Β°C | Caffeine | Paraxanthine | NADPH regenerating system (containing NADP+, glucose-6-phosphate and glucose-6-phosphate-dehydrogenase), no clear concentration | Yes, "shaking water bath" | Classical aqueous control (in nmol/min/ΞΌM/nmol CYP) | Enzymes in supersomes |
| Activity - Michaelis-Menten | Vmax/kM (in nmol/min/ΞΌM/nmol CYP) measured by LC/MS after incubation (20 minutes at 37Β°C) in the presence of organic solvent | Ethanol | 2% | 0.16 | 0.38 | nmol/min/ΞΌM/nmol CYP | 100 mM potassium phosphate buffer | 7.4 | 37Β°C | Caffeine | Paraxanthine | NADPH regenerating system (containing NADP+, glucose-6-phosphate and glucose-6-phosphate-dehydrogenase), no clear concentration | Yes, "shaking water bath" | Classical aqueous control (in nmol/min/ΞΌM/nmol CYP) | Enzymes in supersomes |
| Activity - Michaelis-Menten | Vmax/kM (in nmol/min/ΞΌM/nmol CYP) measured by LC/MS after incubation (20 minutes at 37Β°C) in the presence of organic solvent | Ethanol | 3% | 0.16 | 0.22 | nmol/min/ΞΌM/nmol CYP | 100 mM potassium phosphate buffer | 7.4 | 37Β°C | Caffeine | Paraxanthine | NADPH regenerating system (containing NADP+, glucose-6-phosphate and glucose-6-phosphate-dehydrogenase), no clear concentration | Yes, "shaking water bath" | Classical aqueous control (in nmol/min/ΞΌM/nmol CYP) | Enzymes in supersomes |
| Activity - Michaelis-Menten | Vmax/kM (in nmol/min/ΞΌM/nmol CYP) measured by LC/MS after incubation (20 minutes at 37Β°C) in the presence of organic solvent | Ethanol | 4% | 0.16 | 0.21 | nmol/min/ΞΌM/nmol CYP | 100 mM potassium phosphate buffer | 7.4 | 37Β°C | Caffeine | Paraxanthine | NADPH regenerating system (containing NADP+, glucose-6-phosphate and glucose-6-phosphate-dehydrogenase), no clear concentration | Yes, "shaking water bath" | Classical aqueous control (in nmol/min/ΞΌM/nmol CYP) | Enzymes in supersomes |
Visualization : Activity β Michaelis-Menten
One bar per measurement. Colour = solvent, shade = solvent volume.