Cytochrome P450 1A2 from Homo sapiens

Enzyme Description

Species
Homo sapiens (human) β†—
Extremophile
No
EC Number
1.14.14.1 β†—

Sequence

Length: 516 amino acids
MALSQSVPFSATELLLASAIFCLVFWVLKGLRPRVPKGLKSPPEPWGWPLLGHVLTLGKNPHLALSRMSQRYGDVLQIRIGSTPVLVLSRLDTIRQALVRQGDDFKGRPDLYTSTLITDGQSLTFSTDSGPVWAARRRLAQNALNTFSIASDPASSSSCYLEEHVSKEAKALISRLQELMAGPGHFDPYNQVVVSVANVIGAMCFGQHFPESSDEMLSLVKNTHEFVETASSGNPLDFFPILRYLPNPALQRFKAFNQRFLWFLQKTVQEHYQDFDKNSVRDITGALFKHSKKGPRASGNLIPQEKIVNLVNDIFGAGFDTVTTAISWSLMYLVTKPEIQRKIQKELDTVIGRERRPRLSDRPQLPYLEAFILETFRHSSFLPFTIPHSTTRDTTLNGFYIPKKCCVFVNQWQVNHDPELWEDPSEFRPERFLTADGTAINKPLSEKMMLFGMGKRRCIGEVLAKWEIFLFLAILLQQLEFSVPPGVKVDLTPIYGLTMKHARCEHVQARLRFSIN
Dennis Rokitta et al. (2013) β€” The effect of organic solvents on enzyme kinetic parameters of human CYP3A4 and CYP1A2 in vitro
Toxicology Mechanisms and Methods  Β· doi:10.3109/15376516.2013.806622 β†—  Β· Activity - Michaelis-Menten
48 measurements ▼
Database ID
UniProt: P05177 β†—
Sequence Annotation
Inferred - from protein name
Protein Source
Commercially purchased

Experimental Data (48 measurements)

48 measurements β€” page 2 of 3
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity - Michaelis-Menten Vmax/kM (in nmol/min/ΞΌM/nmol CYP) measured by LC/MS after incubation (20 minutes at 37Β°C) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 1% 0.16 0.21 nmol/min/ΞΌM/nmol CYP 100 mM potassium phosphate buffer 7.4 37Β°C Caffeine Paraxanthine NADPH regenerating system (containing NADP+, glucose-6-phosphate and glucose-6-phosphate-dehydrogenase), no clear concentration Yes, "shaking water bath" Classical aqueous control (in nmol/min/ΞΌM/nmol CYP) | Enzymes in supersomes
Activity - Michaelis-Menten Vmax/kM (in nmol/min/ΞΌM/nmol CYP) measured by LC/MS after incubation (20 minutes at 37Β°C) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 2% 0.16 0.34 nmol/min/ΞΌM/nmol CYP 100 mM potassium phosphate buffer 7.4 37Β°C Caffeine Paraxanthine NADPH regenerating system (containing NADP+, glucose-6-phosphate and glucose-6-phosphate-dehydrogenase), no clear concentration Yes, "shaking water bath" Classical aqueous control (in nmol/min/ΞΌM/nmol CYP) | Enzymes in supersomes
Activity - Michaelis-Menten Vmax/kM (in nmol/min/ΞΌM/nmol CYP) measured by LC/MS after incubation (20 minutes at 37Β°C) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 3% 0.16 0.28 nmol/min/ΞΌM/nmol CYP 100 mM potassium phosphate buffer 7.4 37Β°C Caffeine Paraxanthine NADPH regenerating system (containing NADP+, glucose-6-phosphate and glucose-6-phosphate-dehydrogenase), no clear concentration Yes, "shaking water bath" Classical aqueous control (in nmol/min/ΞΌM/nmol CYP) | Enzymes in supersomes
Activity - Michaelis-Menten Vmax/kM (in nmol/min/ΞΌM/nmol CYP) measured by LC/MS after incubation (20 minutes at 37Β°C) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 4% 0.16 0.4 nmol/min/ΞΌM/nmol CYP 100 mM potassium phosphate buffer 7.4 37Β°C Caffeine Paraxanthine NADPH regenerating system (containing NADP+, glucose-6-phosphate and glucose-6-phosphate-dehydrogenase), no clear concentration Yes, "shaking water bath" Classical aqueous control (in nmol/min/ΞΌM/nmol CYP) | Enzymes in supersomes
Activity - Michaelis-Menten Km (in Β΅M) measured by LC/MS after incubation (20 minutes at 37Β°C) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 1% 1100.0 632.0 Β΅M 100 mM potassium phosphate buffer 7.4 37Β°C Caffeine Paraxanthine NADPH regenerating system (containing NADP+, glucose-6-phosphate and glucose-6-phosphate-dehydrogenase), no clear concentration Yes, "shaking water bath" Classical aqueous control (in microM) | Enzymes in supersomes
Activity - Michaelis-Menten Km (in Β΅M) measured by LC/MS after incubation (20 minutes at 37Β°C) in the presence of organic solvent Acetonitrile 2% 1100.0 368.0 Β΅M 100 mM potassium phosphate buffer 7.4 37Β°C Caffeine Paraxanthine NADPH regenerating system (containing NADP+, glucose-6-phosphate and glucose-6-phosphate-dehydrogenase), no clear concentration Yes, "shaking water bath" Classical aqueous control (in microM) | Enzymes in supersomes
Activity - Michaelis-Menten Km (in Β΅M) measured by LC/MS after incubation (20 minutes at 37Β°C) in the presence of organic solvent Acetonitrile 3% 1100.0 495.0 Β΅M 100 mM potassium phosphate buffer 7.4 37Β°C Caffeine Paraxanthine NADPH regenerating system (containing NADP+, glucose-6-phosphate and glucose-6-phosphate-dehydrogenase), no clear concentration Yes, "shaking water bath" Classical aqueous control (in microM) | Enzymes in supersomes
Activity - Michaelis-Menten Km (in Β΅M) measured by LC/MS after incubation (20 minutes at 37Β°C) in the presence of organic solvent Acetonitrile 4% 1100.0 877.0 Β΅M 100 mM potassium phosphate buffer 7.4 37Β°C Caffeine Paraxanthine NADPH regenerating system (containing NADP+, glucose-6-phosphate and glucose-6-phosphate-dehydrogenase), no clear concentration Yes, "shaking water bath" Classical aqueous control (in microM) | Enzymes in supersomes
Activity - Michaelis-Menten Km (in Β΅M) measured by LC/MS after incubation (20 minutes at 37Β°C) in the presence of organic solvent Methanol 1% 1100.0 401.0 Β΅M 100 mM potassium phosphate buffer 7.4 37Β°C Caffeine Paraxanthine NADPH regenerating system (containing NADP+, glucose-6-phosphate and glucose-6-phosphate-dehydrogenase), no clear concentration Yes, "shaking water bath" Classical aqueous control (in microM) | Enzymes in supersomes
Activity - Michaelis-Menten Km (in Β΅M) measured by LC/MS after incubation (20 minutes at 37Β°C) in the presence of organic solvent Methanol 2% 1100.0 260.0 Β΅M 100 mM potassium phosphate buffer 7.4 37Β°C Caffeine Paraxanthine NADPH regenerating system (containing NADP+, glucose-6-phosphate and glucose-6-phosphate-dehydrogenase), no clear concentration Yes, "shaking water bath" Classical aqueous control (in microM) | Enzymes in supersomes
Activity - Michaelis-Menten Km (in Β΅M) measured by LC/MS after incubation (20 minutes at 37Β°C) in the presence of organic solvent Methanol 3% 1100.0 319.0 Β΅M 100 mM potassium phosphate buffer 7.4 37Β°C Caffeine Paraxanthine NADPH regenerating system (containing NADP+, glucose-6-phosphate and glucose-6-phosphate-dehydrogenase), no clear concentration Yes, "shaking water bath" Classical aqueous control (in microM) | Enzymes in supersomes
Activity - Michaelis-Menten Km (in Β΅M) measured by LC/MS after incubation (20 minutes at 37Β°C) in the presence of organic solvent Methanol 4% 1100.0 285.0 Β΅M 100 mM potassium phosphate buffer 7.4 37Β°C Caffeine Paraxanthine NADPH regenerating system (containing NADP+, glucose-6-phosphate and glucose-6-phosphate-dehydrogenase), no clear concentration Yes, "shaking water bath" Classical aqueous control (in microM) | Enzymes in supersomes
Activity - Michaelis-Menten Km (in Β΅M) measured by LC/MS after incubation (20 minutes at 37Β°C) in the presence of organic solvent Ethanol 1% 1100.0 153.0 Β΅M 100 mM potassium phosphate buffer 7.4 37Β°C Caffeine Paraxanthine NADPH regenerating system (containing NADP+, glucose-6-phosphate and glucose-6-phosphate-dehydrogenase), no clear concentration Yes, "shaking water bath" Classical aqueous control (in microM) | Enzymes in supersomes
Activity - Michaelis-Menten Km (in Β΅M) measured by LC/MS after incubation (20 minutes at 37Β°C) in the presence of organic solvent Ethanol 2% 1100.0 191.0 Β΅M 100 mM potassium phosphate buffer 7.4 37Β°C Caffeine Paraxanthine NADPH regenerating system (containing NADP+, glucose-6-phosphate and glucose-6-phosphate-dehydrogenase), no clear concentration Yes, "shaking water bath" Classical aqueous control (in microM) | Enzymes in supersomes
Activity - Michaelis-Menten Km (in Β΅M) measured by LC/MS after incubation (20 minutes at 37Β°C) in the presence of organic solvent Ethanol 3% 1100.0 307.0 Β΅M 100 mM potassium phosphate buffer 7.4 37Β°C Caffeine Paraxanthine NADPH regenerating system (containing NADP+, glucose-6-phosphate and glucose-6-phosphate-dehydrogenase), no clear concentration Yes, "shaking water bath" Classical aqueous control (in microM) | Enzymes in supersomes
Activity - Michaelis-Menten Km (in Β΅M) measured by LC/MS after incubation (20 minutes at 37Β°C) in the presence of organic solvent Ethanol 4% 1100.0 238.0 Β΅M 100 mM potassium phosphate buffer 7.4 37Β°C Caffeine Paraxanthine NADPH regenerating system (containing NADP+, glucose-6-phosphate and glucose-6-phosphate-dehydrogenase), no clear concentration Yes, "shaking water bath" Classical aqueous control (in microM) | Enzymes in supersomes
Activity - Michaelis-Menten Km (in Β΅M) measured by LC/MS after incubation (20 minutes at 37Β°C) in the presence of organic solvent Acetonitrile 1% 1100.0 555.0 Β΅M 100 mM potassium phosphate buffer 7.4 37Β°C Caffeine Paraxanthine NADPH regenerating system (containing NADP+, glucose-6-phosphate and glucose-6-phosphate-dehydrogenase), no clear concentration Yes, "shaking water bath" Classical aqueous control (in microM) | Enzymes in supersomes
Activity - Michaelis-Menten Km (in Β΅M) measured by LC/MS after incubation (20 minutes at 37Β°C) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 2% 1100.0 295.0 Β΅M 100 mM potassium phosphate buffer 7.4 37Β°C Caffeine Paraxanthine NADPH regenerating system (containing NADP+, glucose-6-phosphate and glucose-6-phosphate-dehydrogenase), no clear concentration Yes, "shaking water bath" Classical aqueous control (in microM) | Enzymes in supersomes
Activity - Michaelis-Menten Km (in Β΅M) measured by LC/MS after incubation (20 minutes at 37Β°C) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 3% 1100.0 535.0 Β΅M 100 mM potassium phosphate buffer 7.4 37Β°C Caffeine Paraxanthine NADPH regenerating system (containing NADP+, glucose-6-phosphate and glucose-6-phosphate-dehydrogenase), no clear concentration Yes, "shaking water bath" Classical aqueous control (in microM) | Enzymes in supersomes
Activity - Michaelis-Menten Km (in Β΅M) measured by LC/MS after incubation (20 minutes at 37Β°C) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 4% 1100.0 176.0 Β΅M 100 mM potassium phosphate buffer 7.4 37Β°C Caffeine Paraxanthine NADPH regenerating system (containing NADP+, glucose-6-phosphate and glucose-6-phosphate-dehydrogenase), no clear concentration Yes, "shaking water bath" Classical aqueous control (in microM) | Enzymes in supersomes
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Visualization : Activity β€” Michaelis-Menten

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Structure

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