Cytochrome P450 1A2 from Homo sapiens

Enzyme Description

Species

Extremophile

EC Number

1.14.14.1 ↗

Sequence

Length: 516 amino acids

MALSQSVPFSATELLLASAIFCLVFWVLKGLRPRVPKGLKSPPEPWGWPLLGHVLTLGKNPHLALSRMSQRYGDVLQIRIGSTPVLVLSRLDTIRQALVRQGDDFKGRPDLYTSTLITDGQSLTFSTDSGPVWAARRRLAQNALNTFSIASDPASSSSCYLEEHVSKEAKALISRLQELMAGPGHFDPYNQVVVSVANVIGAMCFGQHFPESSDEMLSLVKNTHEFVETASSGNPLDFFPILRYLPNPALQRFKAFNQRFLWFLQKTVQEHYQDFDKNSVRDITGALFKHSKKGPRASGNLIPQEKIVNLVNDIFGAGFDTVTTAISWSLMYLVTKPEIQRKIQKELDTVIGRERRPRLSDRPQLPYLEAFILETFRHSSFLPFTIPHSTTRDTTLNGFYIPKKCCVFVNQWQVNHDPELWEDPSEFRPERFLTADGTAINKPLSEKMMLFGMGKRRCIGEVLAKWEIFLFLAILLQQLEFSVPPGVKVDLTPIYGLTMKHARCEHVQARLRFSIN

Dennis Rokitta et al. (2013) — The effect of organic solvents on enzyme kinetic parameters of human CYP3A4 and CYP1A2 in vitro

Toxicology Mechanisms and Methods · doi:10.3109/15376516.2013.806622 ↗ · Activity - Michaelis-Menten

48 measurements ▼

Database ID

UniProt: P05177 ↗

Sequence Annotation

Inferred - from protein name

Protein Source

Commercially purchased

Experimental Data (48 measurements)

48 measurements — page 3 of 3

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Activity - Michaelis-Menten	Vmax (in nmol/min/nmol CYP) measured by LC/MS after incubation (20 minutes at 37°C) in the presence of organic solvent	Acetonitrile	1%	172.0	1052.0	nmol/min/nmol CYP	100 mM potassium phosphate buffer	7.4	37°C	Caffeine	Paraxanthine	NADPH regenerating system (containing NADP+, glucose-6-phosphate and glucose-6-phosphate-dehydrogenase), no clear concentration	Yes, "shaking water bath"	Classical aqueous control (in nmol/min/nmol CYP) \| Enzymes in supersomes
Activity - Michaelis-Menten	Vmax (in nmol/min/nmol CYP) measured by LC/MS after incubation (20 minutes at 37°C) in the presence of organic solvent	Acetonitrile	2%	172.0	932.0	nmol/min/nmol CYP	100 mM potassium phosphate buffer	7.4	37°C	Caffeine	Paraxanthine	NADPH regenerating system (containing NADP+, glucose-6-phosphate and glucose-6-phosphate-dehydrogenase), no clear concentration	Yes, "shaking water bath"	Classical aqueous control (in nmol/min/nmol CYP) \| Enzymes in supersomes
Activity - Michaelis-Menten	Vmax (in nmol/min/nmol CYP) measured by LC/MS after incubation (20 minutes at 37°C) in the presence of organic solvent	Acetonitrile	3%	172.0	946.0	nmol/min/nmol CYP	100 mM potassium phosphate buffer	7.4	37°C	Caffeine	Paraxanthine	NADPH regenerating system (containing NADP+, glucose-6-phosphate and glucose-6-phosphate-dehydrogenase), no clear concentration	Yes, "shaking water bath"	Classical aqueous control (in nmol/min/nmol CYP) \| Enzymes in supersomes
Activity - Michaelis-Menten	Vmax (in nmol/min/nmol CYP) measured by LC/MS after incubation (20 minutes at 37°C) in the presence of organic solvent	Acetonitrile	4%	172.0	926.0	nmol/min/nmol CYP	100 mM potassium phosphate buffer	7.4	37°C	Caffeine	Paraxanthine	NADPH regenerating system (containing NADP+, glucose-6-phosphate and glucose-6-phosphate-dehydrogenase), no clear concentration	Yes, "shaking water bath"	Classical aqueous control (in nmol/min/nmol CYP) \| Enzymes in supersomes
Activity - Michaelis-Menten	Vmax (in nmol/min/nmol CYP) measured by LC/MS after incubation (20 minutes at 37°C) in the presence of organic solvent	Methanol	1%	172.0	66.0	nmol/min/nmol CYP	100 mM potassium phosphate buffer	7.4	37°C	Caffeine	Paraxanthine	NADPH regenerating system (containing NADP+, glucose-6-phosphate and glucose-6-phosphate-dehydrogenase), no clear concentration	Yes, "shaking water bath"	Classical aqueous control (in nmol/min/nmol CYP) \| Enzymes in supersomes
Activity - Michaelis-Menten	Vmax (in nmol/min/nmol CYP) measured by LC/MS after incubation (20 minutes at 37°C) in the presence of organic solvent	Methanol	2%	172.0	53.0	nmol/min/nmol CYP	100 mM potassium phosphate buffer	7.4	37°C	Caffeine	Paraxanthine	NADPH regenerating system (containing NADP+, glucose-6-phosphate and glucose-6-phosphate-dehydrogenase), no clear concentration	Yes, "shaking water bath"	Classical aqueous control (in nmol/min/nmol CYP) \| Enzymes in supersomes
Activity - Michaelis-Menten	Vmax (in nmol/min/nmol CYP) measured by LC/MS after incubation (20 minutes at 37°C) in the presence of organic solvent	Methanol	3%	172.0	63.0	nmol/min/nmol CYP	100 mM potassium phosphate buffer	7.4	37°C	Caffeine	Paraxanthine	NADPH regenerating system (containing NADP+, glucose-6-phosphate and glucose-6-phosphate-dehydrogenase), no clear concentration	Yes, "shaking water bath"	Classical aqueous control (in nmol/min/nmol CYP) \| Enzymes in supersomes
Activity - Michaelis-Menten	Vmax (in nmol/min/nmol CYP) measured by LC/MS after incubation (20 minutes at 37°C) in the presence of organic solvent	Methanol	4%	172.0	54.0	nmol/min/nmol CYP	100 mM potassium phosphate buffer	7.4	37°C	Caffeine	Paraxanthine	NADPH regenerating system (containing NADP+, glucose-6-phosphate and glucose-6-phosphate-dehydrogenase), no clear concentration	Yes, "shaking water bath"	Classical aqueous control (in nmol/min/nmol CYP) \| Enzymes in supersomes

Activity - Michaelis-Menten

Vmax/kM (in nmol/min/μM/nmol CYP) measured by LC/MS after incubation (20 minutes at 37°C) in the presence of organic solvent

Methanol

0.16

nmol/min/μM/nmol CYP

100 mM potassium phosphate buffer

7.4

37°C

Caffeine

Paraxanthine

NADPH regenerating system (containing NADP+, glucose-6-phosphate and glucose-6-phosphate-dehydrogenase), no clear concentration

Yes, "shaking water bath"

Classical aqueous control (in nmol/min/μM/nmol CYP) | Enzymes in supersomes

Activity - Michaelis-Menten

Vmax (in nmol/min/nmol CYP) measured by LC/MS after incubation (20 minutes at 37°C) in the presence of organic solvent

Ethanol

172.0

72.0

nmol/min/nmol CYP

100 mM potassium phosphate buffer

7.4

37°C

Caffeine

Paraxanthine

NADPH regenerating system (containing NADP+, glucose-6-phosphate and glucose-6-phosphate-dehydrogenase), no clear concentration

Yes, "shaking water bath"

Classical aqueous control (in nmol/min/nmol CYP) | Enzymes in supersomes

Activity - Michaelis-Menten

Vmax (in nmol/min/nmol CYP) measured by LC/MS after incubation (20 minutes at 37°C) in the presence of organic solvent

Ethanol

172.0

68.0

nmol/min/nmol CYP

100 mM potassium phosphate buffer

7.4

37°C

Caffeine

Paraxanthine

NADPH regenerating system (containing NADP+, glucose-6-phosphate and glucose-6-phosphate-dehydrogenase), no clear concentration

Yes, "shaking water bath"

Classical aqueous control (in nmol/min/nmol CYP) | Enzymes in supersomes

Activity - Michaelis-Menten

Vmax (in nmol/min/nmol CYP) measured by LC/MS after incubation (20 minutes at 37°C) in the presence of organic solvent

Ethanol

172.0

51.0

nmol/min/nmol CYP

100 mM potassium phosphate buffer

7.4

37°C

Caffeine

Paraxanthine

NADPH regenerating system (containing NADP+, glucose-6-phosphate and glucose-6-phosphate-dehydrogenase), no clear concentration

Yes, "shaking water bath"

Classical aqueous control (in nmol/min/nmol CYP) | Enzymes in supersomes

Activity - Michaelis-Menten

Vmax (in nmol/min/nmol CYP) measured by LC/MS after incubation (20 minutes at 37°C) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

172.0

135.0

nmol/min/nmol CYP

100 mM potassium phosphate buffer

7.4

37°C

Caffeine

Paraxanthine

NADPH regenerating system (containing NADP+, glucose-6-phosphate and glucose-6-phosphate-dehydrogenase), no clear concentration

Yes, "shaking water bath"

Classical aqueous control (in nmol/min/nmol CYP) | Enzymes in supersomes

Activity - Michaelis-Menten

Vmax (in nmol/min/nmol CYP) measured by LC/MS after incubation (20 minutes at 37°C) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

172.0

101.0

nmol/min/nmol CYP

100 mM potassium phosphate buffer

7.4

37°C

Caffeine

Paraxanthine

NADPH regenerating system (containing NADP+, glucose-6-phosphate and glucose-6-phosphate-dehydrogenase), no clear concentration

Yes, "shaking water bath"

Classical aqueous control (in nmol/min/nmol CYP) | Enzymes in supersomes

Activity - Michaelis-Menten

Vmax (in nmol/min/nmol CYP) measured by LC/MS after incubation (20 minutes at 37°C) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

172.0

149.0

nmol/min/nmol CYP

100 mM potassium phosphate buffer

7.4

37°C

Caffeine

Paraxanthine

NADPH regenerating system (containing NADP+, glucose-6-phosphate and glucose-6-phosphate-dehydrogenase), no clear concentration

Yes, "shaking water bath"

Classical aqueous control (in nmol/min/nmol CYP) | Enzymes in supersomes

Activity - Michaelis-Menten

Vmax (in nmol/min/nmol CYP) measured by LC/MS after incubation (20 minutes at 37°C) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

172.0

71.0

nmol/min/nmol CYP

100 mM potassium phosphate buffer

7.4

37°C

Caffeine

Paraxanthine

NADPH regenerating system (containing NADP+, glucose-6-phosphate and glucose-6-phosphate-dehydrogenase), no clear concentration

Yes, "shaking water bath"

Classical aqueous control (in nmol/min/nmol CYP) | Enzymes in supersomes

Activity - Michaelis-Menten

Vmax/kM (in nmol/min/μM/nmol CYP) measured by LC/MS after incubation (20 minutes at 37°C) in the presence of organic solvent

Acetonitrile

0.16

1.9

nmol/min/μM/nmol CYP

100 mM potassium phosphate buffer

7.4

37°C

Caffeine

Paraxanthine

NADPH regenerating system (containing NADP+, glucose-6-phosphate and glucose-6-phosphate-dehydrogenase), no clear concentration

Yes, "shaking water bath"

Classical aqueous control (in nmol/min/μM/nmol CYP) | Enzymes in supersomes

Activity - Michaelis-Menten

Vmax/kM (in nmol/min/μM/nmol CYP) measured by LC/MS after incubation (20 minutes at 37°C) in the presence of organic solvent

Acetonitrile

0.16

2.53

nmol/min/μM/nmol CYP

100 mM potassium phosphate buffer

7.4

37°C

Caffeine

Paraxanthine

NADPH regenerating system (containing NADP+, glucose-6-phosphate and glucose-6-phosphate-dehydrogenase), no clear concentration

Yes, "shaking water bath"

Classical aqueous control (in nmol/min/μM/nmol CYP) | Enzymes in supersomes

Activity - Michaelis-Menten

Vmax/kM (in nmol/min/μM/nmol CYP) measured by LC/MS after incubation (20 minutes at 37°C) in the presence of organic solvent

Acetonitrile

0.16

1.91

nmol/min/μM/nmol CYP

100 mM potassium phosphate buffer

7.4

37°C

Caffeine

Paraxanthine

NADPH regenerating system (containing NADP+, glucose-6-phosphate and glucose-6-phosphate-dehydrogenase), no clear concentration

Yes, "shaking water bath"

Classical aqueous control (in nmol/min/μM/nmol CYP) | Enzymes in supersomes

Activity - Michaelis-Menten

Vmax/kM (in nmol/min/μM/nmol CYP) measured by LC/MS after incubation (20 minutes at 37°C) in the presence of organic solvent

Acetonitrile

0.16

1.06

nmol/min/μM/nmol CYP

100 mM potassium phosphate buffer

7.4

37°C

Caffeine

Paraxanthine

NADPH regenerating system (containing NADP+, glucose-6-phosphate and glucose-6-phosphate-dehydrogenase), no clear concentration

Yes, "shaking water bath"

Classical aqueous control (in nmol/min/μM/nmol CYP) | Enzymes in supersomes

Activity - Michaelis-Menten

Vmax (in nmol/min/nmol CYP) measured by LC/MS after incubation (20 minutes at 37°C) in the presence of organic solvent

Ethanol

172.0

66.0

nmol/min/nmol CYP

100 mM potassium phosphate buffer

7.4

37°C

Caffeine

Paraxanthine

NADPH regenerating system (containing NADP+, glucose-6-phosphate and glucose-6-phosphate-dehydrogenase), no clear concentration

Yes, "shaking water bath"

Classical aqueous control (in nmol/min/nmol CYP) | Enzymes in supersomes

Activity - Michaelis-Menten

Vmax/kM (in nmol/min/μM/nmol CYP) measured by LC/MS after incubation (20 minutes at 37°C) in the presence of organic solvent

Methanol

0.16

0.2

nmol/min/μM/nmol CYP

100 mM potassium phosphate buffer

7.4

37°C

Caffeine

Paraxanthine

NADPH regenerating system (containing NADP+, glucose-6-phosphate and glucose-6-phosphate-dehydrogenase), no clear concentration

Yes, "shaking water bath"

Classical aqueous control (in nmol/min/μM/nmol CYP) | Enzymes in supersomes

Activity - Michaelis-Menten

Vmax/kM (in nmol/min/μM/nmol CYP) measured by LC/MS after incubation (20 minutes at 37°C) in the presence of organic solvent

Methanol

0.16

0.2

nmol/min/μM/nmol CYP

100 mM potassium phosphate buffer

7.4

37°C

Caffeine

Paraxanthine

NADPH regenerating system (containing NADP+, glucose-6-phosphate and glucose-6-phosphate-dehydrogenase), no clear concentration

Yes, "shaking water bath"

Classical aqueous control (in nmol/min/μM/nmol CYP) | Enzymes in supersomes

Activity - Michaelis-Menten

Vmax/kM (in nmol/min/μM/nmol CYP) measured by LC/MS after incubation (20 minutes at 37°C) in the presence of organic solvent

Methanol

0.16

0.19

nmol/min/μM/nmol CYP

100 mM potassium phosphate buffer

7.4

37°C

Caffeine

Paraxanthine

NADPH regenerating system (containing NADP+, glucose-6-phosphate and glucose-6-phosphate-dehydrogenase), no clear concentration

Yes, "shaking water bath"

Classical aqueous control (in nmol/min/μM/nmol CYP) | Enzymes in supersomes

Activity - Michaelis-Menten

Vmax/kM (in nmol/min/μM/nmol CYP) measured by LC/MS after incubation (20 minutes at 37°C) in the presence of organic solvent

Ethanol

0.16

0.43

nmol/min/μM/nmol CYP

100 mM potassium phosphate buffer

7.4

37°C

Caffeine

Paraxanthine

NADPH regenerating system (containing NADP+, glucose-6-phosphate and glucose-6-phosphate-dehydrogenase), no clear concentration

Yes, "shaking water bath"

Classical aqueous control (in nmol/min/μM/nmol CYP) | Enzymes in supersomes

Activity - Michaelis-Menten

Vmax/kM (in nmol/min/μM/nmol CYP) measured by LC/MS after incubation (20 minutes at 37°C) in the presence of organic solvent

Ethanol

0.16

0.38

nmol/min/μM/nmol CYP

100 mM potassium phosphate buffer

7.4

37°C

Caffeine

Paraxanthine

NADPH regenerating system (containing NADP+, glucose-6-phosphate and glucose-6-phosphate-dehydrogenase), no clear concentration

Yes, "shaking water bath"

Classical aqueous control (in nmol/min/μM/nmol CYP) | Enzymes in supersomes

Activity - Michaelis-Menten

Vmax/kM (in nmol/min/μM/nmol CYP) measured by LC/MS after incubation (20 minutes at 37°C) in the presence of organic solvent

Ethanol

0.16

0.22

nmol/min/μM/nmol CYP

100 mM potassium phosphate buffer

7.4

37°C

Caffeine

Paraxanthine

NADPH regenerating system (containing NADP+, glucose-6-phosphate and glucose-6-phosphate-dehydrogenase), no clear concentration

Yes, "shaking water bath"

Classical aqueous control (in nmol/min/μM/nmol CYP) | Enzymes in supersomes

Activity - Michaelis-Menten

Vmax/kM (in nmol/min/μM/nmol CYP) measured by LC/MS after incubation (20 minutes at 37°C) in the presence of organic solvent

Ethanol

0.16

0.21

nmol/min/μM/nmol CYP

100 mM potassium phosphate buffer

7.4

37°C

Caffeine

Paraxanthine

NADPH regenerating system (containing NADP+, glucose-6-phosphate and glucose-6-phosphate-dehydrogenase), no clear concentration

Yes, "shaking water bath"

Classical aqueous control (in nmol/min/μM/nmol CYP) | Enzymes in supersomes

Activity - Michaelis-Menten

Vmax/kM (in nmol/min/μM/nmol CYP) measured by LC/MS after incubation (20 minutes at 37°C) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

0.16

0.21

nmol/min/μM/nmol CYP

100 mM potassium phosphate buffer

7.4

37°C

Caffeine

Paraxanthine

NADPH regenerating system (containing NADP+, glucose-6-phosphate and glucose-6-phosphate-dehydrogenase), no clear concentration

Yes, "shaking water bath"

Classical aqueous control (in nmol/min/μM/nmol CYP) | Enzymes in supersomes

Activity - Michaelis-Menten

Vmax/kM (in nmol/min/μM/nmol CYP) measured by LC/MS after incubation (20 minutes at 37°C) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

0.16

0.34

nmol/min/μM/nmol CYP

100 mM potassium phosphate buffer

7.4

37°C

Caffeine

Paraxanthine

NADPH regenerating system (containing NADP+, glucose-6-phosphate and glucose-6-phosphate-dehydrogenase), no clear concentration

Yes, "shaking water bath"

Classical aqueous control (in nmol/min/μM/nmol CYP) | Enzymes in supersomes

Activity - Michaelis-Menten

Vmax/kM (in nmol/min/μM/nmol CYP) measured by LC/MS after incubation (20 minutes at 37°C) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

0.16

0.28

nmol/min/μM/nmol CYP

100 mM potassium phosphate buffer

7.4

37°C

Caffeine

Paraxanthine

NADPH regenerating system (containing NADP+, glucose-6-phosphate and glucose-6-phosphate-dehydrogenase), no clear concentration

Yes, "shaking water bath"

Classical aqueous control (in nmol/min/μM/nmol CYP) | Enzymes in supersomes

Activity - Michaelis-Menten

Vmax/kM (in nmol/min/μM/nmol CYP) measured by LC/MS after incubation (20 minutes at 37°C) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

0.16

0.4

nmol/min/μM/nmol CYP

100 mM potassium phosphate buffer

7.4

37°C

Caffeine

Paraxanthine

NADPH regenerating system (containing NADP+, glucose-6-phosphate and glucose-6-phosphate-dehydrogenase), no clear concentration

Yes, "shaking water bath"

Classical aqueous control (in nmol/min/μM/nmol CYP) | Enzymes in supersomes

Activity - Michaelis-Menten

Km (in µM) measured by LC/MS after incubation (20 minutes at 37°C) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

1100.0

632.0

µM

100 mM potassium phosphate buffer

7.4

37°C

Caffeine

Paraxanthine

NADPH regenerating system (containing NADP+, glucose-6-phosphate and glucose-6-phosphate-dehydrogenase), no clear concentration

Yes, "shaking water bath"

Classical aqueous control (in µM) | Enzymes in supersomes

Activity - Michaelis-Menten

Km (in µM) measured by LC/MS after incubation (20 minutes at 37°C) in the presence of organic solvent

Acetonitrile

1100.0

368.0

µM

100 mM potassium phosphate buffer

7.4

37°C

Caffeine

Paraxanthine

NADPH regenerating system (containing NADP+, glucose-6-phosphate and glucose-6-phosphate-dehydrogenase), no clear concentration

Yes, "shaking water bath"

Classical aqueous control (in µM) | Enzymes in supersomes

Activity - Michaelis-Menten

Km (in µM) measured by LC/MS after incubation (20 minutes at 37°C) in the presence of organic solvent

Acetonitrile

1100.0

495.0

µM

100 mM potassium phosphate buffer

7.4

37°C

Caffeine

Paraxanthine

NADPH regenerating system (containing NADP+, glucose-6-phosphate and glucose-6-phosphate-dehydrogenase), no clear concentration

Yes, "shaking water bath"

Classical aqueous control (in µM) | Enzymes in supersomes

Activity - Michaelis-Menten

Km (in µM) measured by LC/MS after incubation (20 minutes at 37°C) in the presence of organic solvent

Acetonitrile

1100.0

877.0

µM

100 mM potassium phosphate buffer

7.4

37°C

Caffeine

Paraxanthine

NADPH regenerating system (containing NADP+, glucose-6-phosphate and glucose-6-phosphate-dehydrogenase), no clear concentration

Yes, "shaking water bath"

Classical aqueous control (in µM) | Enzymes in supersomes

Activity - Michaelis-Menten

Km (in µM) measured by LC/MS after incubation (20 minutes at 37°C) in the presence of organic solvent

Methanol

1100.0

401.0

µM

100 mM potassium phosphate buffer

7.4

37°C

Caffeine

Paraxanthine

NADPH regenerating system (containing NADP+, glucose-6-phosphate and glucose-6-phosphate-dehydrogenase), no clear concentration

Yes, "shaking water bath"

Classical aqueous control (in µM) | Enzymes in supersomes

Activity - Michaelis-Menten

Km (in µM) measured by LC/MS after incubation (20 minutes at 37°C) in the presence of organic solvent

Methanol

1100.0

260.0

µM

100 mM potassium phosphate buffer

7.4

37°C

Caffeine

Paraxanthine

NADPH regenerating system (containing NADP+, glucose-6-phosphate and glucose-6-phosphate-dehydrogenase), no clear concentration

Yes, "shaking water bath"

Classical aqueous control (in µM) | Enzymes in supersomes

Activity - Michaelis-Menten

Km (in µM) measured by LC/MS after incubation (20 minutes at 37°C) in the presence of organic solvent

Methanol

1100.0

319.0

µM

100 mM potassium phosphate buffer

7.4

37°C

Caffeine

Paraxanthine

NADPH regenerating system (containing NADP+, glucose-6-phosphate and glucose-6-phosphate-dehydrogenase), no clear concentration

Yes, "shaking water bath"

Classical aqueous control (in µM) | Enzymes in supersomes

Activity - Michaelis-Menten

Km (in µM) measured by LC/MS after incubation (20 minutes at 37°C) in the presence of organic solvent

Methanol

1100.0

285.0

µM

100 mM potassium phosphate buffer

7.4

37°C

Caffeine

Paraxanthine

NADPH regenerating system (containing NADP+, glucose-6-phosphate and glucose-6-phosphate-dehydrogenase), no clear concentration

Yes, "shaking water bath"

Classical aqueous control (in µM) | Enzymes in supersomes

Activity - Michaelis-Menten

Km (in µM) measured by LC/MS after incubation (20 minutes at 37°C) in the presence of organic solvent

Ethanol

1100.0

153.0

µM

100 mM potassium phosphate buffer

7.4

37°C

Caffeine

Paraxanthine

NADPH regenerating system (containing NADP+, glucose-6-phosphate and glucose-6-phosphate-dehydrogenase), no clear concentration

Yes, "shaking water bath"

Classical aqueous control (in µM) | Enzymes in supersomes

Activity - Michaelis-Menten

Km (in µM) measured by LC/MS after incubation (20 minutes at 37°C) in the presence of organic solvent

Ethanol

1100.0

191.0

µM

100 mM potassium phosphate buffer

7.4

37°C

Caffeine

Paraxanthine

NADPH regenerating system (containing NADP+, glucose-6-phosphate and glucose-6-phosphate-dehydrogenase), no clear concentration

Yes, "shaking water bath"

Classical aqueous control (in µM) | Enzymes in supersomes

Activity - Michaelis-Menten

Km (in µM) measured by LC/MS after incubation (20 minutes at 37°C) in the presence of organic solvent

Ethanol

1100.0

307.0

µM

100 mM potassium phosphate buffer

7.4

37°C

Caffeine

Paraxanthine

NADPH regenerating system (containing NADP+, glucose-6-phosphate and glucose-6-phosphate-dehydrogenase), no clear concentration

Yes, "shaking water bath"

Classical aqueous control (in µM) | Enzymes in supersomes

Activity - Michaelis-Menten

Km (in µM) measured by LC/MS after incubation (20 minutes at 37°C) in the presence of organic solvent

Ethanol

1100.0

238.0

µM

100 mM potassium phosphate buffer

7.4

37°C

Caffeine

Paraxanthine

NADPH regenerating system (containing NADP+, glucose-6-phosphate and glucose-6-phosphate-dehydrogenase), no clear concentration

Yes, "shaking water bath"

Classical aqueous control (in µM) | Enzymes in supersomes

Activity - Michaelis-Menten

Km (in µM) measured by LC/MS after incubation (20 minutes at 37°C) in the presence of organic solvent

Acetonitrile

1100.0

555.0

µM

100 mM potassium phosphate buffer

7.4

37°C

Caffeine

Paraxanthine

NADPH regenerating system (containing NADP+, glucose-6-phosphate and glucose-6-phosphate-dehydrogenase), no clear concentration

Yes, "shaking water bath"

Classical aqueous control (in µM) | Enzymes in supersomes

Activity - Michaelis-Menten

Km (in µM) measured by LC/MS after incubation (20 minutes at 37°C) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

1100.0

295.0

µM

100 mM potassium phosphate buffer

7.4

37°C

Caffeine

Paraxanthine

NADPH regenerating system (containing NADP+, glucose-6-phosphate and glucose-6-phosphate-dehydrogenase), no clear concentration

Yes, "shaking water bath"

Classical aqueous control (in µM) | Enzymes in supersomes

Activity - Michaelis-Menten

Km (in µM) measured by LC/MS after incubation (20 minutes at 37°C) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

1100.0

535.0

µM

100 mM potassium phosphate buffer

7.4

37°C

Caffeine

Paraxanthine

NADPH regenerating system (containing NADP+, glucose-6-phosphate and glucose-6-phosphate-dehydrogenase), no clear concentration

Yes, "shaking water bath"

Classical aqueous control (in µM) | Enzymes in supersomes

Activity - Michaelis-Menten

Km (in µM) measured by LC/MS after incubation (20 minutes at 37°C) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

1100.0

176.0

µM

100 mM potassium phosphate buffer

7.4

37°C

Caffeine

Paraxanthine

NADPH regenerating system (containing NADP+, glucose-6-phosphate and glucose-6-phosphate-dehydrogenase), no clear concentration

Yes, "shaking water bath"

Classical aqueous control (in µM) | Enzymes in supersomes

Activity - Michaelis-Menten

Vmax (in nmol/min/nmol CYP) measured by LC/MS after incubation (20 minutes at 37°C) in the presence of organic solvent

Acetonitrile

172.0

1052.0

nmol/min/nmol CYP

100 mM potassium phosphate buffer

7.4

37°C

Caffeine

Paraxanthine

NADPH regenerating system (containing NADP+, glucose-6-phosphate and glucose-6-phosphate-dehydrogenase), no clear concentration

Yes, "shaking water bath"

Classical aqueous control (in nmol/min/nmol CYP) | Enzymes in supersomes

Activity - Michaelis-Menten

Vmax (in nmol/min/nmol CYP) measured by LC/MS after incubation (20 minutes at 37°C) in the presence of organic solvent

Acetonitrile

172.0

932.0

nmol/min/nmol CYP

100 mM potassium phosphate buffer

7.4

37°C

Caffeine

Paraxanthine

NADPH regenerating system (containing NADP+, glucose-6-phosphate and glucose-6-phosphate-dehydrogenase), no clear concentration

Yes, "shaking water bath"

Classical aqueous control (in nmol/min/nmol CYP) | Enzymes in supersomes

Activity - Michaelis-Menten

Vmax (in nmol/min/nmol CYP) measured by LC/MS after incubation (20 minutes at 37°C) in the presence of organic solvent

Acetonitrile

172.0

946.0

nmol/min/nmol CYP

100 mM potassium phosphate buffer

7.4

37°C

Caffeine

Paraxanthine

NADPH regenerating system (containing NADP+, glucose-6-phosphate and glucose-6-phosphate-dehydrogenase), no clear concentration

Yes, "shaking water bath"

Classical aqueous control (in nmol/min/nmol CYP) | Enzymes in supersomes

Activity - Michaelis-Menten

Vmax (in nmol/min/nmol CYP) measured by LC/MS after incubation (20 minutes at 37°C) in the presence of organic solvent

Acetonitrile

172.0

926.0

nmol/min/nmol CYP

100 mM potassium phosphate buffer

7.4

37°C

Caffeine

Paraxanthine

NADPH regenerating system (containing NADP+, glucose-6-phosphate and glucose-6-phosphate-dehydrogenase), no clear concentration

Yes, "shaking water bath"

Classical aqueous control (in nmol/min/nmol CYP) | Enzymes in supersomes

Activity - Michaelis-Menten

Vmax (in nmol/min/nmol CYP) measured by LC/MS after incubation (20 minutes at 37°C) in the presence of organic solvent

Methanol

172.0

66.0

nmol/min/nmol CYP

100 mM potassium phosphate buffer

7.4

37°C

Caffeine

Paraxanthine

NADPH regenerating system (containing NADP+, glucose-6-phosphate and glucose-6-phosphate-dehydrogenase), no clear concentration

Yes, "shaking water bath"

Classical aqueous control (in nmol/min/nmol CYP) | Enzymes in supersomes

Activity - Michaelis-Menten

Vmax (in nmol/min/nmol CYP) measured by LC/MS after incubation (20 minutes at 37°C) in the presence of organic solvent

Methanol

172.0

53.0

nmol/min/nmol CYP

100 mM potassium phosphate buffer

7.4

37°C

Caffeine

Paraxanthine

NADPH regenerating system (containing NADP+, glucose-6-phosphate and glucose-6-phosphate-dehydrogenase), no clear concentration

Yes, "shaking water bath"

Classical aqueous control (in nmol/min/nmol CYP) | Enzymes in supersomes

Activity - Michaelis-Menten

Vmax (in nmol/min/nmol CYP) measured by LC/MS after incubation (20 minutes at 37°C) in the presence of organic solvent

Methanol

172.0

63.0

nmol/min/nmol CYP

100 mM potassium phosphate buffer

7.4

37°C

Caffeine

Paraxanthine

NADPH regenerating system (containing NADP+, glucose-6-phosphate and glucose-6-phosphate-dehydrogenase), no clear concentration

Yes, "shaking water bath"

Classical aqueous control (in nmol/min/nmol CYP) | Enzymes in supersomes

Activity - Michaelis-Menten

Vmax (in nmol/min/nmol CYP) measured by LC/MS after incubation (20 minutes at 37°C) in the presence of organic solvent

Methanol

172.0

54.0

nmol/min/nmol CYP

100 mM potassium phosphate buffer

7.4

37°C

Caffeine

Paraxanthine

NADPH regenerating system (containing NADP+, glucose-6-phosphate and glucose-6-phosphate-dehydrogenase), no clear concentration

Yes, "shaking water bath"

Classical aqueous control (in nmol/min/nmol CYP) | Enzymes in supersomes

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Visualization : Activity — Michaelis-Menten

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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Cytochrome P450 1A2 from Homo sapiens

Enzyme Description

Sequence

Experimental Data (48 measurements)

Visualization : Activity — Michaelis-Menten

Structure

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