Aldo-keto reductase from Candida tropicalis MYA-3404

Enzyme Description

Species
Candida tropicalis MYA-3404 β†—
Extremophile
No
EC Number
1.1.1.2 β†—

Sequence

Length: 350 amino acids
MSFTQFFNTYSDNQLVQIGLIFALLIGVLNLTIFLLKFGSLIYDLFLAPAVDFSKYGAGNGKWAVVTGASDGIGKEYALQLAKKGLSIVLVSRTQSKLELIAKEIETTYKVNTKIVAFDASTDDEENYLTLEKAIFDLPITVLINNVGQSHSIPVPFLETTKKELKDIITINNTATLRITQIVAPVIVSTVERSPQKSLRGLILTMGSFGGLLPTPYLATYSGSKAFLQSWSSALAGELAGDNVDVELVISYLVTSAMSKIKRSSVTIPNPKQFVRSTLSSVGRRGGAQERYATSTPYWSHALMEFGIESTVGVYSKIANKLNLQMHQSIRHRALKKAARLAAAAAEKND
Wei Jiang et al. (2021) β€” Gene mining, codon optimization and analysis of binding mechanism of an aldo-keto reductase with high activity, better substrate specificity and excellent solvent tolerance
PLOS ONE  Β· doi:10.1371/journal.pone.0260787.g004 β†—  Β· Activity - Classical Stability - Incubation
54 measurements ▼
Database ID
UniProt: C5M3I1 β†—
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (54 measurements)

54 measurements β€” page 3 of 3
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (4 hours at room temperature), measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in aqueous phase Methanol 10% 100 89 % Incubation: ?, Assay: PBS buffer Incubation: ?, Assay: 7.4 Incubation: Room temperature, Assay: Room temperature ? mM Acetylacetone 4-Hydroxypentan-2-one ? mM NADPH β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (5 hours at room temperature), measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in aqueous phase Methanol 10% 100 77 % Incubation: ?, Assay: PBS buffer Incubation: ?, Assay: 7.4 Incubation: Room temperature, Assay: Room temperature ? mM Acetylacetone 4-Hydroxypentan-2-one ? mM NADPH β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (6 hours at room temperature), measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in aqueous phase Methanol 10% 100 70 % Incubation: ?, Assay: PBS buffer Incubation: ?, Assay: 7.4 Incubation: Room temperature, Assay: Room temperature ? mM Acetylacetone 4-Hydroxypentan-2-one ? mM NADPH β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in aqueous phase Ethanol 10% 100 84 % Incubation: ?, Assay: PBS buffer Incubation: ?, Assay: 7.4 Incubation: Room temperature, Assay: Room temperature ? mM Acetylacetone 4-Hydroxypentan-2-one ? mM NADPH β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (2 hours at room temperature), measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in aqueous phase Ethanol 10% 100 83 % Incubation: ?, Assay: PBS buffer Incubation: ?, Assay: 7.4 Incubation: Room temperature, Assay: Room temperature ? mM Acetylacetone 4-Hydroxypentan-2-one ? mM NADPH β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (6 hours at room temperature), measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in aqueous phase Isopropanol 10% 100 84 % Incubation: ?, Assay: PBS buffer Incubation: ?, Assay: 7.4 Incubation: Room temperature, Assay: Room temperature ? mM Acetylacetone 4-Hydroxypentan-2-one ? mM NADPH β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (4 hours at room temperature), measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in aqueous phase Ethanol 10% 100 88 % Incubation: ?, Assay: PBS buffer Incubation: ?, Assay: 7.4 Incubation: Room temperature, Assay: Room temperature ? mM Acetylacetone 4-Hydroxypentan-2-one ? mM NADPH β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (5 hours at room temperature), measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in aqueous phase Ethanol 10% 100 81 % Incubation: ?, Assay: PBS buffer Incubation: ?, Assay: 7.4 Incubation: Room temperature, Assay: Room temperature ? mM Acetylacetone 4-Hydroxypentan-2-one ? mM NADPH β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (6 hours at room temperature), measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in aqueous phase Ethanol 10% 100 79 % Incubation: ?, Assay: PBS buffer Incubation: ?, Assay: 7.4 Incubation: Room temperature, Assay: Room temperature ? mM Acetylacetone 4-Hydroxypentan-2-one ? mM NADPH β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in aqueous phase Isopropanol 10% 100 87 % Incubation: ?, Assay: PBS buffer Incubation: ?, Assay: 7.4 Incubation: Room temperature, Assay: Room temperature ? mM Acetylacetone 4-Hydroxypentan-2-one ? mM NADPH β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (2 hours at room temperature), measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in aqueous phase Isopropanol 10% 100 90 % Incubation: ?, Assay: PBS buffer Incubation: ?, Assay: 7.4 Incubation: Room temperature, Assay: Room temperature ? mM Acetylacetone 4-Hydroxypentan-2-one ? mM NADPH β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (3 hours at room temperature), measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in aqueous phase Isopropanol 10% 100 87 % Incubation: ?, Assay: PBS buffer Incubation: ?, Assay: 7.4 Incubation: Room temperature, Assay: Room temperature ? mM Acetylacetone 4-Hydroxypentan-2-one ? mM NADPH β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (4 hours at room temperature), measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in aqueous phase Isopropanol 10% 100 82 % Incubation: ?, Assay: PBS buffer Incubation: ?, Assay: 7.4 Incubation: Room temperature, Assay: Room temperature ? mM Acetylacetone 4-Hydroxypentan-2-one ? mM NADPH β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (5 hours at room temperature), measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in aqueous phase Isopropanol 10% 100 88 % Incubation: ?, Assay: PBS buffer Incubation: ?, Assay: 7.4 Incubation: Room temperature, Assay: Room temperature ? mM Acetylacetone 4-Hydroxypentan-2-one ? mM NADPH β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
β€Ή Prev
1 2 3
Next β€Ί

Visualization : Activity β€” Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

Drag to rotate Β· Scroll to zoom Β· Right-click drag to pan Β· Powered by Mol*