Aldo-keto reductase from Candida tropicalis MYA-3404
Enzyme Description
Sequence
MSFTQFFNTYSDNQLVQIGLIFALLIGVLNLTIFLLKFGSLIYDLFLAPAVDFSKYGAGNGKWAVVTGASDGIGKEYALQLAKKGLSIVLVSRTQSKLELIAKEIETTYKVNTKIVAFDASTDDEENYLTLEKAIFDLPITVLINNVGQSHSIPVPFLETTKKELKDIITINNTATLRITQIVAPVIVSTVERSPQKSLRGLILTMGSFGGLLPTPYLATYSGSKAFLQSWSSALAGELAGDNVDVELVISYLVTSAMSKIKRSSVTIPNPKQFVRSTLSSVGRRGGAQERYATSTPYWSHALMEFGIESTVGVYSKIANKLNLQMHQSIRHRALKKAARLAAAAAEKND
Wei Jiang et al. (2021)
β Gene mining, codon optimization and analysis of binding mechanism of an aldo-keto reductase with high activity, better substrate specificity and excellent solvent tolerance
PLOS ONE
Β· doi:10.1371/journal.pone.0260787.g004 β
Β· Activity - Classical Stability - Incubation
▼
Database ID
UniProt: C5M3I1 β
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)
Experimental Data (54 measurements)
54 measurements β page 2 of 3
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Stability - Incubation | Activity after incubation (3 hours at room temperature), measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in aqueous phase | Ethyl Acetate | 10% | 100 | 100 | % | Incubation: ?, Assay: PBS buffer | Incubation: ?, Assay: 7.4 | Incubation: Room temperature, Assay: Room temperature | ? mM Acetylacetone | 4-Hydroxypentan-2-one | ? mM NADPH | β | Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase |
| Stability - Incubation | Activity after incubation (4 hours at room temperature), measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in aqueous phase | Ethyl Acetate | 10% | 100 | 91 | % | Incubation: ?, Assay: PBS buffer | Incubation: ?, Assay: 7.4 | Incubation: Room temperature, Assay: Room temperature | ? mM Acetylacetone | 4-Hydroxypentan-2-one | ? mM NADPH | β | Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase |
| Stability - Incubation | Activity after incubation (5 hours at room temperature), measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in aqueous phase | Ethyl Acetate | 10% | 100 | 92 | % | Incubation: ?, Assay: PBS buffer | Incubation: ?, Assay: 7.4 | Incubation: Room temperature, Assay: Room temperature | ? mM Acetylacetone | 4-Hydroxypentan-2-one | ? mM NADPH | β | Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase |
| Stability - Incubation | Activity after incubation (6 hours at room temperature), measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in aqueous phase | Ethyl Acetate | 10% | 100 | 98 | % | Incubation: ?, Assay: PBS buffer | Incubation: ?, Assay: 7.4 | Incubation: Room temperature, Assay: Room temperature | ? mM Acetylacetone | 4-Hydroxypentan-2-one | ? mM NADPH | β | Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase |
| Stability - Incubation | Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in aqueous phase | Acetonitrile | 10% | 100 | 83 | % | Incubation: ?, Assay: PBS buffer | Incubation: ?, Assay: 7.4 | Incubation: Room temperature, Assay: Room temperature | ? mM Acetylacetone | 4-Hydroxypentan-2-one | ? mM NADPH | β | Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase |
| Stability - Incubation | Activity after incubation (2 hours at room temperature), measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in aqueous phase | Acetonitrile | 10% | 100 | 89 | % | Incubation: ?, Assay: PBS buffer | Incubation: ?, Assay: 7.4 | Incubation: Room temperature, Assay: Room temperature | ? mM Acetylacetone | 4-Hydroxypentan-2-one | ? mM NADPH | β | Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase |
| Stability - Incubation | Activity after incubation (3 hours at room temperature), measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in aqueous phase | Acetonitrile | 10% | 100 | 84 | % | Incubation: ?, Assay: PBS buffer | Incubation: ?, Assay: 7.4 | Incubation: Room temperature, Assay: Room temperature | ? mM Acetylacetone | 4-Hydroxypentan-2-one | ? mM NADPH | β | Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase |
| Stability - Incubation | Activity after incubation (4 hours at room temperature), measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in aqueous phase | Acetonitrile | 10% | 100 | 84 | % | Incubation: ?, Assay: PBS buffer | Incubation: ?, Assay: 7.4 | Incubation: Room temperature, Assay: Room temperature | ? mM Acetylacetone | 4-Hydroxypentan-2-one | ? mM NADPH | β | Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase |
| Stability - Incubation | Activity after incubation (5 hours at room temperature), measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) | Acetonitrile | 10% | 100 | 82 | % | Incubation: ?, Assay: PBS buffer | Incubation: ?, Assay: 7.4 | Incubation: Room temperature, Assay: Room temperature | ? mM Acetylacetone | 4-Hydroxypentan-2-one | ? mM NADPH | β | Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase |
| Stability - Incubation | Activity after incubation (6 hours at room temperature), measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in aqueous phase | Acetonitrile | 10% | 100 | 82 | % | Incubation: ?, Assay: PBS buffer | Incubation: ?, Assay: 7.4 | Incubation: Room temperature, Assay: Room temperature | ? mM Acetylacetone | 4-Hydroxypentan-2-one | ? mM NADPH | β | Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase |
| Stability - Incubation | Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 10% | 100 | 78 | % | Incubation: ?, Assay: PBS buffer | Incubation: ?, Assay: 7.4 | Incubation: Room temperature, Assay: Room temperature | ? mM Acetylacetone | 4-Hydroxypentan-2-one | ? mM NADPH | β | Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase |
| Stability - Incubation | Activity after incubation (2 hours at room temperature), measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 10% | 100 | 87 | % | Incubation: ?, Assay: PBS buffer | Incubation: ?, Assay: 7.4 | Incubation: Room temperature, Assay: Room temperature | ? mM Acetylacetone | 4-Hydroxypentan-2-one | ? mM NADPH | β | Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase |
| Stability - Incubation | Activity after incubation (3 hours at room temperature), measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 10% | 100 | 89 | % | Incubation: ?, Assay: PBS buffer | Incubation: ?, Assay: 7.4 | Incubation: Room temperature, Assay: Room temperature | ? mM Acetylacetone | 4-Hydroxypentan-2-one | ? mM NADPH | β | Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase |
| Stability - Incubation | Activity after incubation (4 hours at room temperature), measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 10% | 100 | 90 | % | Incubation: ?, Assay: PBS buffer | Incubation: ?, Assay: 7.4 | Incubation: Room temperature, Assay: Room temperature | ? mM Acetylacetone | 4-Hydroxypentan-2-one | ? mM NADPH | β | Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase |
| Stability - Incubation | Activity after incubation (5 hours at room temperature), measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 10% | 100 | 90 | % | Incubation: ?, Assay: PBS buffer | Incubation: ?, Assay: 7.4 | Incubation: Room temperature, Assay: Room temperature | ? mM Acetylacetone | 4-Hydroxypentan-2-one | ? mM NADPH | β | Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase |
| Stability - Incubation | Activity after incubation (6 hours at room temperature), measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 10% | 100 | 81 | % | Incubation: ?, Assay: PBS buffer | Incubation: ?, Assay: 7.4 | Incubation: Room temperature, Assay: Room temperature | ? mM Acetylacetone | 4-Hydroxypentan-2-one | ? mM NADPH | β | Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase |
| Stability - Incubation | Activity after incubation (3 hours at room temperature), measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in aqueous phase | Ethanol | 10% | 100 | 87 | % | Incubation: ?, Assay: PBS buffer | Incubation: ?, Assay: 7.4 | Incubation: Room temperature, Assay: Room temperature | ? mM Acetylacetone | 4-Hydroxypentan-2-one | ? mM NADPH | β | Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase |
| Stability - Incubation | Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in aqueous phase | Methanol | 10% | 100 | 82 | % | Incubation: ?, Assay: PBS buffer | Incubation: ?, Assay: 7.4 | Incubation: Room temperature, Assay: Room temperature | ? mM Acetylacetone | 4-Hydroxypentan-2-one | ? mM NADPH | β | Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase |
| Stability - Incubation | Activity after incubation (2 hours at room temperature), measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in aqueous phase | Methanol | 10% | 100 | 91 | % | Incubation: ?, Assay: PBS buffer | Incubation: ?, Assay: 7.4 | Incubation: Room temperature, Assay: Room temperature | ? mM Acetylacetone | 4-Hydroxypentan-2-one | ? mM NADPH | β | Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase |
| Stability - Incubation | Activity after incubation (3 hours at room temperature), measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in aqueous phase | Methanol | 10% | 100 | 92 | % | Incubation: ?, Assay: PBS buffer | Incubation: ?, Assay: 7.4 | Incubation: Room temperature, Assay: Room temperature | ? mM Acetylacetone | 4-Hydroxypentan-2-one | ? mM NADPH | β | Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase |
Visualization : Activity β Classical
One bar per measurement. Colour = solvent, shade = solvent volume.
Visualization : Stability β Incubation
One bar per measurement. Colour = solvent, shade = solvent volume.