Aldo-keto reductase from Candida tropicalis MYA-3404

Enzyme Description

Species
Candida tropicalis MYA-3404 β†—
Extremophile
No
EC Number
1.1.1.2 β†—

Sequence

Length: 350 amino acids
MSFTQFFNTYSDNQLVQIGLIFALLIGVLNLTIFLLKFGSLIYDLFLAPAVDFSKYGAGNGKWAVVTGASDGIGKEYALQLAKKGLSIVLVSRTQSKLELIAKEIETTYKVNTKIVAFDASTDDEENYLTLEKAIFDLPITVLINNVGQSHSIPVPFLETTKKELKDIITINNTATLRITQIVAPVIVSTVERSPQKSLRGLILTMGSFGGLLPTPYLATYSGSKAFLQSWSSALAGELAGDNVDVELVISYLVTSAMSKIKRSSVTIPNPKQFVRSTLSSVGRRGGAQERYATSTPYWSHALMEFGIESTVGVYSKIANKLNLQMHQSIRHRALKKAARLAAAAAEKND
Wei Jiang et al. (2021) β€” Gene mining, codon optimization and analysis of binding mechanism of an aldo-keto reductase with high activity, better substrate specificity and excellent solvent tolerance
PLOS ONE  Β· doi:10.1371/journal.pone.0260787.g004 β†—  Β· Activity - Classical Stability - Incubation
54 measurements ▼
Database ID
UniProt: C5M3I1 β†—
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (54 measurements)

54 measurements β€” page 1 of 3
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Methanol 20% 100 87 % PBS buffer 7.4 Room temperature ? mM Acetylacetone 4-Hydroxypentan-2-one ? mM NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Methanol 10% 100 80 % PBS buffer 7.4 Room temperature ? mM Acetylacetone 4-Hydroxypentan-2-one ? mM NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 30% 100 41 % PBS buffer 7.4 Room temperature ? mM Acetylacetone 4-Hydroxypentan-2-one ? mM NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 20% 100 50 % PBS buffer 7.4 Room temperature ? mM Acetylacetone 4-Hydroxypentan-2-one ? mM NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 10% 100 93 % PBS buffer 7.4 Room temperature ? mM Acetylacetone 4-Hydroxypentan-2-one ? mM NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Acetonitrile 30% 100 90 % PBS buffer 7.4 Room temperature ? mM Acetylacetone 4-Hydroxypentan-2-one ? mM NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Acetonitrile 20% 100 79 % PBS buffer 7.4 Room temperature ? mM Acetylacetone 4-Hydroxypentan-2-one ? mM NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Acetonitrile 10% 100 91 % PBS buffer 7.4 Room temperature ? mM Acetylacetone 4-Hydroxypentan-2-one ? mM NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Ethyl Acetate 30% 100 76 % PBS buffer 7.4 Room temperature ? mM Acetylacetone 4-Hydroxypentan-2-one ? mM NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Ethyl Acetate 20% 100 95 % PBS buffer 7.4 Room temperature ? mM Acetylacetone 4-Hydroxypentan-2-one ? mM NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Isopropanol 30% 100 95 % PBS buffer 7.4 Room temperature ? mM Acetylacetone 4-Hydroxypentan-2-one ? mM NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Ethyl Acetate 10% 100 91 % PBS buffer 7.4 Room temperature ? mM Acetylacetone 4-Hydroxypentan-2-one ? mM NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Methanol 30% 100 97 % PBS buffer 7.4 Room temperature ? mM Acetylacetone 4-Hydroxypentan-2-one ? mM NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Ethanol 10% 100 66 % PBS buffer 7.4 Room temperature ? mM Acetylacetone 4-Hydroxypentan-2-one ? mM NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Ethanol 20% 100 72 % PBS buffer 7.4 Room temperature ? mM Acetylacetone 4-Hydroxypentan-2-one ? mM NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Ethanol 30% 100 97 % PBS buffer 7.4 Room temperature ? mM Acetylacetone 4-Hydroxypentan-2-one ? mM NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Isopropanol 10% 100 76 % PBS buffer 7.4 Room temperature ? mM Acetylacetone 4-Hydroxypentan-2-one ? mM NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Isopropanol 20% 100 97 % PBS buffer 7.4 Room temperature ? mM Acetylacetone 4-Hydroxypentan-2-one ? mM NADPH β€” Classical aqueous control (in %)
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in aqueous phase Ethyl Acetate 10% 100 87 % Incubation: ?, Assay: PBS buffer Incubation: ?, Assay: 7.4 Incubation: Room temperature, Assay: Room temperature ? mM Acetylacetone 4-Hydroxypentan-2-one ? mM NADPH β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (2 hours at room temperature), measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) Ethyl Acetate 10% 100 95 % Incubation: ?, Assay: PBS buffer Incubation: ?, Assay: 7.4 Incubation: Room temperature, Assay: Room temperature ? mM Acetylacetone 4-Hydroxypentan-2-one ? mM NADPH β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
β€Ή Prev
1 2 3
Next β€Ί

Visualization : Activity β€” Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

Drag to rotate Β· Scroll to zoom Β· Right-click drag to pan Β· Powered by Mol*