Thermolysin from Bacillus thermoproteolyticus

Enzyme Description

Species

Bacillus thermoproteolyticus ↗

Extremophile

Yes "thermophilic" organism

EC Number

3.4.24.27 ↗

Sequence

Length: 316 amino acids

ITGTSTVGVGRGVLGDQKNINTTYSTYYYLQDNTRGNGIFTYDAKYRTTLPGSLWADADNQFFASYDAPAVDAHYYAGVTYDYYKNVHNRLSYDGNNAAIRSSVHYSQGYNNAFWNGSQMVYGDGDGQTFIPLSGGIDVVAHELTHAVTDYTAGLIYQNESGAINEAISDIFGTLVEFYANKNPDWEIGEDVYTPGISGDSLRSMSDPAKYGDPDHYSKRYTGTQDNGGVHINSGIINKAAYLISQGGTHYGVSVVGIGRDKLGKIFYRALTQYLTPTSNFSQLRAAAVQSATDLYGSTSQEVASVKQAFDAVGVK

Kohei Oda et al. (1996) — A Novel Alcohol Resistant Metalloproteinase, Vimelysin, from Vibrio sp. T1800: Purification and Characterization

Bioscience, Biotechnology, and Biochemistry · doi:10.1271/bbb.60.463 ↗ · Activity - Classical

22 measurements ▼

Database ID

UniProt: P00800 ↗

Sequence Annotation

Inferred - from protein name (first 232 residues from UniProt sequence absent from the mature protein)

Protein Source

Commercially purchased

Experimental Data (22 measurements)

22 measurements — page 1 of 2

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Activity - Classical	Activity measured by absorbance spectrophotometry (FAGLA absorbance measurement, 345 nm) in the presence of organic solvent	Isopropanol	10%	100	1	%	0.1 M MES	6.5	?	1 mM FAGLA (FA-Gly-DL-Leu-NH2)	Furylacryloyl-Gly , Leu-NH2	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (FAGLA absorbance measurement, 345 nm) in the presence of organic solvent	Dimethylformamide (DMF)	30%	100	1	%	0.1 M MES	6.5	?	1 mM FAGLA (FA-Gly-DL-Leu-NH2)	Furylacryloyl-Gly , Leu-NH2	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (FAGLA absorbance measurement, 345 nm) in the presence of organic solvent	Dimethylformamide (DMF)	20%	100	6	%	0.1 M MES	6.5	?	1 mM FAGLA (FA-Gly-DL-Leu-NH2)	Furylacryloyl-Gly , Leu-NH2	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (FAGLA absorbance measurement, 345 nm) in the presence of organic solvent	Dimethylformamide (DMF)	10%	100	21	%	0.1 M MES	6.5	?	1 mM FAGLA (FA-Gly-DL-Leu-NH2)	Furylacryloyl-Gly , Leu-NH2	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (FAGLA absorbance measurement, 345 nm) in the presence of organic solvent	Dimethylformamide (DMF)	5%	100	44	%	0.1 M MES	6.5	?	1 mM FAGLA (FA-Gly-DL-Leu-NH2)	Furylacryloyl-Gly , Leu-NH2	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (FAGLA absorbance measurement, 345 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	30%	100	0	%	0.1 M MES	6.5	?	1 mM FAGLA (FA-Gly-DL-Leu-NH2)	Furylacryloyl-Gly , Leu-NH2	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (FAGLA absorbance measurement, 345 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	20%	100	11	%	0.1 M MES	6.5	?	1 mM FAGLA (FA-Gly-DL-Leu-NH2)	Furylacryloyl-Gly , Leu-NH2	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (FAGLA absorbance measurement, 345 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10%	100	69	%	0.1 M MES	6.5	?	1 mM FAGLA (FA-Gly-DL-Leu-NH2)	Furylacryloyl-Gly , Leu-NH2	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (FAGLA absorbance measurement, 345 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	5%	100	95	%	0.1 M MES	6.5	?	1 mM FAGLA (FA-Gly-DL-Leu-NH2)	Furylacryloyl-Gly , Leu-NH2	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (FAGLA absorbance measurement, 345 nm) in the presence of organic solvent	Isopropanol	30%	100	0	%	0.1 M MES	6.5	?	1 mM FAGLA (FA-Gly-DL-Leu-NH2)	Furylacryloyl-Gly , Leu-NH2	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (FAGLA absorbance measurement, 345 nm) in the presence of organic solvent	Isopropanol	20%	100	0	%	0.1 M MES	6.5	?	1 mM FAGLA (FA-Gly-DL-Leu-NH2)	Furylacryloyl-Gly , Leu-NH2	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (FAGLA absorbance measurement, 345 nm) in the presence of organic solvent	Methanol	10%	100	24	%	0.1 M MES	6.5	?	1 mM FAGLA (FA-Gly-DL-Leu-NH2)	Furylacryloyl-Gly , Leu-NH2	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (FAGLA absorbance measurement, 345 nm) in the presence of organic solvent	Isopropanol	5%	100	10	%	0.1 M MES	6.5	?	1 mM FAGLA (FA-Gly-DL-Leu-NH2)	Furylacryloyl-Gly , Leu-NH2	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (FAGLA absorbance measurement, 345 nm) in the presence of organic solvent	Isopropanol	30%	100	0	%	0.1 M MES	6.5	?	1 mM FAGLA (FA-Gly-DL-Leu-NH2)	Furylacryloyl-Gly , Leu-NH2	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (FAGLA absorbance measurement, 345 nm) in the presence of organic solvent	Isopropanol	20%	100	0	%	0.1 M MES	6.5	?	1 mM FAGLA (FA-Gly-DL-Leu-NH2)	Furylacryloyl-Gly , Leu-NH2	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (FAGLA absorbance measurement, 345 nm) in the presence of organic solvent	Isopropanol	10%	100	6	%	0.1 M MES	6.5	?	1 mM FAGLA (FA-Gly-DL-Leu-NH2)	Furylacryloyl-Gly , Leu-NH2	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (FAGLA absorbance measurement, 345 nm) in the presence of organic solvent	Isopropanol	5%	100	21	%	0.1 M MES	6.5	?	1 mM FAGLA (FA-Gly-DL-Leu-NH2)	Furylacryloyl-Gly , Leu-NH2	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (FAGLA absorbance measurement, 345 nm) in the presence of organic solvent	Ethanol	30%	100	0	%	0.1 M MES	6.5	?	1 mM FAGLA (FA-Gly-DL-Leu-NH2)	Furylacryloyl-Gly , Leu-NH2	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (FAGLA absorbance measurement, 345 nm) in the presence of organic solvent	Ethanol	20%	100	0	%	0.1 M MES	6.5	?	1 mM FAGLA (FA-Gly-DL-Leu-NH2)	Furylacryloyl-Gly , Leu-NH2	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (FAGLA absorbance measurement, 345 nm) in the presence of organic solvent	Ethanol	10%	100	6	%	0.1 M MES	6.5	?	1 mM FAGLA (FA-Gly-DL-Leu-NH2)	Furylacryloyl-Gly , Leu-NH2	—	—	Classical aqueous control (in %)

Activity - Classical	Activity measured by absorbance spectrophotometry (FAGLA absorbance measurement, 345 nm) in the presence of organic solvent	Isopropanol	10%	100	1	%	0.1 M MES	6.5	?	1 mM FAGLA (FA-Gly-DL-Leu-NH2)	Furylacryloyl-Gly, Leu-NH2	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (FAGLA absorbance measurement, 345 nm) in the presence of organic solvent	Dimethylformamide (DMF)	30%	100	1	%	0.1 M MES	6.5	?	1 mM FAGLA (FA-Gly-DL-Leu-NH2)	Furylacryloyl-Gly, Leu-NH2	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (FAGLA absorbance measurement, 345 nm) in the presence of organic solvent	Dimethylformamide (DMF)	20%	100	6	%	0.1 M MES	6.5	?	1 mM FAGLA (FA-Gly-DL-Leu-NH2)	Furylacryloyl-Gly, Leu-NH2	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (FAGLA absorbance measurement, 345 nm) in the presence of organic solvent	Dimethylformamide (DMF)	10%	100	21	%	0.1 M MES	6.5	?	1 mM FAGLA (FA-Gly-DL-Leu-NH2)	Furylacryloyl-Gly, Leu-NH2	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (FAGLA absorbance measurement, 345 nm) in the presence of organic solvent	Dimethylformamide (DMF)	5%	100	44	%	0.1 M MES	6.5	?	1 mM FAGLA (FA-Gly-DL-Leu-NH2)	Furylacryloyl-Gly, Leu-NH2	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (FAGLA absorbance measurement, 345 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	30%	100	0	%	0.1 M MES	6.5	?	1 mM FAGLA (FA-Gly-DL-Leu-NH2)	Furylacryloyl-Gly, Leu-NH2	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (FAGLA absorbance measurement, 345 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	20%	100	11	%	0.1 M MES	6.5	?	1 mM FAGLA (FA-Gly-DL-Leu-NH2)	Furylacryloyl-Gly, Leu-NH2	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (FAGLA absorbance measurement, 345 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10%	100	69	%	0.1 M MES	6.5	?	1 mM FAGLA (FA-Gly-DL-Leu-NH2)	Furylacryloyl-Gly, Leu-NH2	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (FAGLA absorbance measurement, 345 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	5%	100	95	%	0.1 M MES	6.5	?	1 mM FAGLA (FA-Gly-DL-Leu-NH2)	Furylacryloyl-Gly, Leu-NH2	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (FAGLA absorbance measurement, 345 nm) in the presence of organic solvent	Isopropanol	30%	100	0	%	0.1 M MES	6.5	?	1 mM FAGLA (FA-Gly-DL-Leu-NH2)	Furylacryloyl-Gly, Leu-NH2	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (FAGLA absorbance measurement, 345 nm) in the presence of organic solvent	Isopropanol	20%	100	0	%	0.1 M MES	6.5	?	1 mM FAGLA (FA-Gly-DL-Leu-NH2)	Furylacryloyl-Gly, Leu-NH2	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (FAGLA absorbance measurement, 345 nm) in the presence of organic solvent	Methanol	10%	100	24	%	0.1 M MES	6.5	?	1 mM FAGLA (FA-Gly-DL-Leu-NH2)	Furylacryloyl-Gly, Leu-NH2	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (FAGLA absorbance measurement, 345 nm) in the presence of organic solvent	Isopropanol	5%	100	10	%	0.1 M MES	6.5	?	1 mM FAGLA (FA-Gly-DL-Leu-NH2)	Furylacryloyl-Gly, Leu-NH2	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (FAGLA absorbance measurement, 345 nm) in the presence of organic solvent	Isopropanol	30%	100	0	%	0.1 M MES	6.5	?	1 mM FAGLA (FA-Gly-DL-Leu-NH2)	Furylacryloyl-Gly, Leu-NH2	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (FAGLA absorbance measurement, 345 nm) in the presence of organic solvent	Isopropanol	20%	100	0	%	0.1 M MES	6.5	?	1 mM FAGLA (FA-Gly-DL-Leu-NH2)	Furylacryloyl-Gly, Leu-NH2	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (FAGLA absorbance measurement, 345 nm) in the presence of organic solvent	Isopropanol	10%	100	6	%	0.1 M MES	6.5	?	1 mM FAGLA (FA-Gly-DL-Leu-NH2)	Furylacryloyl-Gly, Leu-NH2	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (FAGLA absorbance measurement, 345 nm) in the presence of organic solvent	Isopropanol	5%	100	21	%	0.1 M MES	6.5	?	1 mM FAGLA (FA-Gly-DL-Leu-NH2)	Furylacryloyl-Gly, Leu-NH2	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (FAGLA absorbance measurement, 345 nm) in the presence of organic solvent	Ethanol	30%	100	0	%	0.1 M MES	6.5	?	1 mM FAGLA (FA-Gly-DL-Leu-NH2)	Furylacryloyl-Gly, Leu-NH2	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (FAGLA absorbance measurement, 345 nm) in the presence of organic solvent	Ethanol	20%	100	0	%	0.1 M MES	6.5	?	1 mM FAGLA (FA-Gly-DL-Leu-NH2)	Furylacryloyl-Gly, Leu-NH2	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (FAGLA absorbance measurement, 345 nm) in the presence of organic solvent	Ethanol	10%	100	6	%	0.1 M MES	6.5	?	1 mM FAGLA (FA-Gly-DL-Leu-NH2)	Furylacryloyl-Gly, Leu-NH2	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (FAGLA absorbance measurement, 345 nm) in the presence of organic solvent	Methanol	30%	100	6	%	0.1 M MES	6.5	?	1 mM FAGLA (FA-Gly-DL-Leu-NH2)	Furylacryloyl-Gly, Leu-NH2	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (FAGLA absorbance measurement, 345 nm) in the presence of organic solvent	Methanol	20%	100	12	%	0.1 M MES	6.5	?	1 mM FAGLA (FA-Gly-DL-Leu-NH2)	Furylacryloyl-Gly, Leu-NH2	—	—	Classical aqueous control (in %)

‹ Prev

1 2

Next ›

Visualization : Activity — Classical

Kohei Oda et al. (1996)

One bar per measurement. Colour = solvent, shade = solvent volume.

Guillermo R. Castro et al. (1999) — Enzymatic activities of proteases dissolved in organic solvents

Enzyme and Microbial Technology · doi:10.1016/S0141-0229(99)00099-X ↗ · Activity - Michaelis-Menten

3 measurements ▼

Database ID

UniProt: P00800 ↗

Sequence Annotation

Inferred - from protein name (first 232 residues from UniProt sequence absent from the mature protein)

Protein Source

Commercially purchased

Experimental Data (3 measurements)

3 measurements

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Activity - Michaelis-Menten	Km (mM) measured by HPLC in the presence of organic solvent	Glycerol	99%	2.5	41.4	mM	20 mM phosphate buffer	7.8	37°C	Phenyl acetate	Acetic Acid , Phenol	—	—	Classical aqueous control (in mM)
Activity - Michaelis-Menten	Vmax (µM/s mg) measured by HPLC in the presence of organic solvent	Glycerol	99%	0.5	0.48	µM/(s * mg)	20 mM phosphate buffer	7.8	37°C	Phenyl acetate	Acetic Acid , Phenol	—	—	Classical aqueous control (in microM/(s * mg))
Activity - Michaelis-Menten	Vm/Km (1/s mg) measured by HPLC in the presence of organic solvent	Glycerol	99%	0.2	0.012	1/(s * mg)	20 mM phosphate buffer	7.8	37°C	Phenyl acetate	Acetic Acid , Phenol	—	—	Classical aqueous control (in 1/(s * mg))

Activity - Michaelis-Menten	Km (mM) measured by HPLC in the presence of organic solvent	Glycerol	99%	2.5	41.4	mM	20 mM phosphate buffer	7.8	37°C	Phenyl acetate	Acetic Acid, Phenol	—	—	Classical aqueous control (in mM)
Activity - Michaelis-Menten	Vmax (µM/s mg) measured by HPLC in the presence of organic solvent	Glycerol	99%	0.5	0.48	µM/(s * mg)	20 mM phosphate buffer	7.8	37°C	Phenyl acetate	Acetic Acid, Phenol	—	—	Classical aqueous control (in µM/(s * mg))
Activity - Michaelis-Menten	Vm/Km (1/s mg) measured by HPLC in the presence of organic solvent	Glycerol	99%	0.2	0.012	1/(s * mg)	20 mM phosphate buffer	7.8	37°C	Phenyl acetate	Acetic Acid, Phenol	—	—	Classical aqueous control (in 1/(s * mg))

Visualization : Activity — Michaelis-Menten

Guillermo R. Castro et al. (1999)

One bar per measurement. Colour = solvent, shade = solvent volume.

Mohammad Pazhang et al. (2006) — Effects of water-miscible solvents and polyhydroxy compounds on the structure and enzymatic activity of thermolysin

Journal of Biotechnology · doi:10.1016/j.jbiotec.2006.05.017 ↗ · Activity - Classical Activity - Michaelis-Menten Stability - Half-life

68 measurements ▼

Database ID

UniProt: P00800 ↗

Sequence Annotation

Inferred - from protein name (first 232 residues from UniProt sequence absent from the mature protein)

Protein Source

Commercially purchased

Experimental Data (68 measurements)

68 measurements — page 4 of 4

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Activity - Michaelis-Menten	K'm (in % Casein) measured by absorbance spectrophotometry (free aromatic amino acids absorbance measurement, 280 nm) in the presence of organic solvent	Isopropanol	26 mM	0.086	0.082	% casein	50 mM Tris-Hcl, 10 mM CaCl2	7.2	25°C	Casein	free amino acids , Peptides	—	—	Classical aqueous control (in % casein)
Activity - Michaelis-Menten	K'm (in % Casein) measured by absorbance spectrophotometry (free aromatic amino acids absorbance measurement, 280 nm) in the presence of organic solvent	Isopropanol	65 mM	0.086	0.091	% casein	50 mM Tris-Hcl, 10 mM CaCl2	7.2	25°C	Casein	free amino acids , Peptides	—	—	Classical aqueous control (in % casein)
Activity - Michaelis-Menten	K'm (in % Casein) measured by absorbance spectrophotometry (free aromatic amino acids absorbance measurement, 280 nm) in the presence of organic solvent	Isopropanol	104 mM	0.086	0.1	% casein	50 mM Tris-Hcl, 10 mM CaCl2	7.2	25°C	Casein	free amino acids , Peptides	—	—	Classical aqueous control (in % casein)
Activity - Michaelis-Menten	K'm (in % Casein) measured by absorbance spectrophotometry (free aromatic amino acids absorbance measurement, 280 nm) in the presence of organic solvent	Dimethylformamide (DMF)	130 mM	0.086	0.082	% casein	50 mM Tris-Hcl, 10 mM CaCl2	7.2	25°C	Casein	free amino acids , Peptides	—	—	Classical aqueous control (in % casein)
Stability - Half-life	Half-life (in minutes) at 80°C, with residual activity measured by absorbance spectrophotometry (free aromatic amino acids absorbance measurement, 280 nm)	1-Propanol	50%	>20.0	3.0	minutes	Incubation: 50 mM Tris-Hcl, 10 mM CaCl2, Assay: 50 mM Tris-Hcl, 10 mM CaCl2	Incubation: 7.2, Assay: 7.2	Incubation: 80°C, Assay: 25°C	0.5% Casein	free amino acids , Peptides	—	—	Classical aqueous control (in minutes)
Stability - Half-life	Half-life (in minutes) at 80°C, with residual activity measured by absorbance spectrophotometry (free aromatic amino acids absorbance measurement, 280 nm)	Isopropanol	50%	>20.0	4.0	minutes	Incubation: 50 mM Tris-Hcl, 10 mM CaCl2, Assay: 50 mM Tris-Hcl, 10 mM CaCl2	Incubation: 7.2, Assay: 7.2	Incubation: 80°C, Assay: 25°C	0.5% Casein	free amino acids , Peptides	—	—	Classical aqueous control (in minutes)
Stability - Half-life	Half-life (in minutes) at 80°C, with residual activity measured by absorbance spectrophotometry (free aromatic amino acids absorbance measurement, 280 nm)	Dimethylformamide (DMF)	50%	>20.0	6.0	minutes	Incubation: 50 mM Tris-Hcl, 10 mM CaCl2, Assay: 50 mM Tris-Hcl, 10 mM CaCl2	Incubation: 7.2, Assay: 7.2	Incubation: 80°C, Assay: 25°C	0.5% Casein	free amino acids , Peptides	—	—	Classical aqueous control (in minutes)
Stability - Half-life	Half-life (in minutes) at 80°C, with residual activity measured by absorbance spectrophotometry (free aromatic amino acids absorbance measurement, 280 nm)	Dimethyl Sulfoxide (DMSO)	50%	>20.0	>20.0	minutes	Incubation: 50 mM Tris-Hcl, 10 mM CaCl2, Assay: 50 mM Tris-Hcl, 10 mM CaCl2	Incubation: 7.2, Assay: 7.2	Incubation: 80°C, Assay: 25°C	0.5% Casein	free amino acids , Peptides	—	—	Classical aqueous control (in minutes)

Activity - Classical

Activity measured by absorbance spectrophotometry (free aromatic amino acids absorbance measurement, 280 nm) in the presence of organic solvent

1-Propanol

100.0

23.0

50 mM Tris-Hcl, 10 mM CaCl2

7.2

25°C

0.5% Casein

free amino acids, Peptides

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (free aromatic amino acids absorbance measurement, 280 nm) in the presence of organic solvent

1-Propanol

100.0

81.0

50 mM Tris-Hcl, 10 mM CaCl2

7.2

25°C

0.5% Casein

free amino acids, Peptides

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (free aromatic amino acids absorbance measurement, 280 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

20%

100.0

20.0

50 mM Tris-Hcl, 10 mM CaCl2

7.2

25°C

0.5% Casein

free amino acids, Peptides

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (free aromatic amino acids absorbance measurement, 280 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

10%

100.0

49.0

50 mM Tris-Hcl, 10 mM CaCl2

7.2

25°C

0.5% Casein

free amino acids, Peptides

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (free aromatic amino acids absorbance measurement, 280 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

100.0

69.0

50 mM Tris-Hcl, 10 mM CaCl2

7.2

25°C

0.5% Casein

free amino acids, Peptides

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (free aromatic amino acids absorbance measurement, 280 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

100.0

89.0

50 mM Tris-Hcl, 10 mM CaCl2

7.2

25°C

0.5% Casein

free amino acids, Peptides

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (free aromatic amino acids absorbance measurement, 280 nm) in the presence of organic solvent

Dimethylformamide (DMF)

20%

100.0

17.0

50 mM Tris-Hcl, 10 mM CaCl2

7.2

25°C

0.5% Casein

free amino acids, Peptides

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (free aromatic amino acids absorbance measurement, 280 nm) in the presence of organic solvent

Dimethylformamide (DMF)

10%

100.0

43.0

50 mM Tris-Hcl, 10 mM CaCl2

7.2

25°C

0.5% Casein

free amino acids, Peptides

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (free aromatic amino acids absorbance measurement, 280 nm) in the presence of organic solvent

Dimethylformamide (DMF)

100.0

65.0

50 mM Tris-Hcl, 10 mM CaCl2

7.2

25°C

0.5% Casein

free amino acids, Peptides

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (free aromatic amino acids absorbance measurement, 280 nm) in the presence of organic solvent

Isopropanol

20%

100.0

1.0

50 mM Tris-Hcl, 10 mM CaCl2

7.2

25°C

0.5% Casein

free amino acids, Peptides

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (free aromatic amino acids absorbance measurement, 280 nm) in the presence of organic solvent

Isopropanol

10%

100.0

7.0

50 mM Tris-Hcl, 10 mM CaCl2

7.2

25°C

0.5% Casein

free amino acids, Peptides

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (free aromatic amino acids absorbance measurement, 280 nm) in the presence of organic solvent

Isopropanol

100.0

22.0

50 mM Tris-Hcl, 10 mM CaCl2

7.2

25°C

0.5% Casein

free amino acids, Peptides

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (free aromatic amino acids absorbance measurement, 280 nm) in the presence of organic solvent

Isopropanol

100.0

65.0

50 mM Tris-Hcl, 10 mM CaCl2

7.2

25°C

0.5% Casein

free amino acids, Peptides

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (free aromatic amino acids absorbance measurement, 280 nm) in the presence of organic solvent

1-Propanol

20%

100.0

1.0

50 mM Tris-Hcl, 10 mM CaCl2

7.2

25°C

0.5% Casein

free amino acids, Peptides

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (free aromatic amino acids absorbance measurement, 280 nm) in the presence of organic solvent

1-Propanol

10%

100.0

3.0

50 mM Tris-Hcl, 10 mM CaCl2

7.2

25°C

0.5% Casein

free amino acids, Peptides

—

Classical aqueous control (in %)

Activity - Classical

Activity measured by absorbance spectrophotometry (free aromatic amino acids absorbance measurement, 280 nm) in the presence of organic solvent

Dimethylformamide (DMF)

100.0

90.0

50 mM Tris-Hcl, 10 mM CaCl2

7.2

25°C

0.5% Casein

free amino acids, Peptides

—

Classical aqueous control (in %)

Activity - Michaelis-Menten

V'm/K'm measured by absorbance spectrophotometry (free aromatic amino acids absorbance measurement, 280 nm) in the presence of organic solvent

1-Propanol

133 mM

1104.0

630.0

amount of released soluble peptide fragments equivalent to 0.001 A280 per minute at standard condition/% casein

50 mM Tris-Hcl, 10 mM CaCl2

7.2

25°C

Casein

free amino acids, Peptides

—

Classical aqueous control (in amount of released soluble peptide fragments equivalent to 0.001 A280 per minute at standard condition/% casein)

Activity - Michaelis-Menten

V'm/K'm measured by absorbance spectrophotometry (free aromatic amino acids absorbance measurement, 280 nm) in the presence of organic solvent

1-Propanol

106.6 mM

1104.0

747.0

amount of released soluble peptide fragments equivalent to 0.001 A280 per minute at standard condition/% casein

50 mM Tris-Hcl, 10 mM CaCl2

7.2

25°C

Casein

free amino acids, Peptides

—

Classical aqueous control (in amount of released soluble peptide fragments equivalent to 0.001 A280 per minute at standard condition/% casein)

Activity - Michaelis-Menten

V'm/K'm measured by absorbance spectrophotometry (free aromatic amino acids absorbance measurement, 280 nm) in the presence of organic solvent

1-Propanol

65.6 mM

1104.0

870.0

amount of released soluble peptide fragments equivalent to 0.001 A280 per minute at standard condition/% casein

50 mM Tris-Hcl, 10 mM CaCl2

7.2

25°C

Casein

free amino acids, Peptides

—

Classical aqueous control (in amount of released soluble peptide fragments equivalent to 0.001 A280 per minute at standard condition/% casein)

Activity - Michaelis-Menten

V'm/K'm measured by absorbance spectrophotometry (free aromatic amino acids absorbance measurement, 280 nm) in the presence of organic solvent

1-Propanol

26.6 mM

1104.0

988.0

amount of released soluble peptide fragments equivalent to 0.001 A280 per minute at standard condition/% casein

50 mM Tris-Hcl, 10 mM CaCl2

7.2

25°C

Casein

free amino acids, Peptides

—

Classical aqueous control (in amount of released soluble peptide fragments equivalent to 0.001 A280 per minute at standard condition/% casein)

Activity - Michaelis-Menten

K'm (in % Casein) measured by absorbance spectrophotometry (free aromatic amino acids absorbance measurement, 280 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

700 mM

0.086

0.067

% casein

50 mM Tris-Hcl, 10 mM CaCl2

7.2

25°C

Casein

free amino acids, Peptides

—

Classical aqueous control (in % casein)

Activity - Michaelis-Menten

K'm (in % Casein) measured by absorbance spectrophotometry (free aromatic amino acids absorbance measurement, 280 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

420 mM

0.086

0.071

% casein

50 mM Tris-Hcl, 10 mM CaCl2

7.2

25°C

Casein

free amino acids, Peptides

—

Classical aqueous control (in % casein)

Activity - Michaelis-Menten

K'm (in % Casein) measured by absorbance spectrophotometry (free aromatic amino acids absorbance measurement, 280 nm) in the presence of organic solvent

Dimethylformamide (DMF)

260 mM

0.086

0.064

% casein

50 mM Tris-Hcl, 10 mM CaCl2

7.2

25°C

Casein

free amino acids, Peptides

—

Classical aqueous control (in % casein)

Activity - Michaelis-Menten

K'm (in % Casein) measured by absorbance spectrophotometry (free aromatic amino acids absorbance measurement, 280 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

280 mM

0.086

0.07

% casein

50 mM Tris-Hcl, 10 mM CaCl2

7.2

25°C

Casein

free amino acids, Peptides

—

Classical aqueous control (in % casein)

Activity - Michaelis-Menten

K'm (in % Casein) measured by absorbance spectrophotometry (free aromatic amino acids absorbance measurement, 280 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

140 mM

0.086

0.077

% casein

50 mM Tris-Hcl, 10 mM CaCl2

7.2

25°C

Casein

free amino acids, Peptides

—

Classical aqueous control (in % casein)

Activity - Michaelis-Menten

K'm (in % Casein) measured by absorbance spectrophotometry (free aromatic amino acids absorbance measurement, 280 nm) in the presence of organic solvent

Dimethylformamide (DMF)

650 mM

0.086

0.056

% casein

50 mM Tris-Hcl, 10 mM CaCl2

7.2

25°C

Casein

free amino acids, Peptides

—

Classical aqueous control (in % casein)

Activity - Michaelis-Menten

K'm (in % Casein) measured by absorbance spectrophotometry (free aromatic amino acids absorbance measurement, 280 nm) in the presence of organic solvent

Dimethylformamide (DMF)

390 mM

0.086

0.065

% casein

50 mM Tris-Hcl, 10 mM CaCl2

7.2

25°C

Casein

free amino acids, Peptides

—

Classical aqueous control (in % casein)

Activity - Michaelis-Menten

K'm (in % Casein) measured by absorbance spectrophotometry (free aromatic amino acids absorbance measurement, 280 nm) in the presence of organic solvent

1-Propanol

106.6 mM

0.086

0.091

% casein

50 mM Tris-Hcl, 10 mM CaCl2

7.2

25°C

Casein

free amino acids, Peptides

—

Classical aqueous control (in % casein)

Activity - Michaelis-Menten

V'm/K'm measured by absorbance spectrophotometry (free aromatic amino acids absorbance measurement, 280 nm) in the presence of organic solvent

Isopropanol

26 mM

1104.0

1024.0

amount of released soluble peptide fragments equivalent to 0.001 A280 per minute at standard condition/% casein

50 mM Tris-Hcl, 10 mM CaCl2

7.2

25°C

Casein

free amino acids, Peptides

—

Classical aqueous control (in amount of released soluble peptide fragments equivalent to 0.001 A280 per minute at standard condition/% casein)

Activity - Michaelis-Menten

V'm/K'm measured by absorbance spectrophotometry (free aromatic amino acids absorbance measurement, 280 nm) in the presence of organic solvent

Isopropanol

65 mM

1104.0

890.0

amount of released soluble peptide fragments equivalent to 0.001 A280 per minute at standard condition/% casein

50 mM Tris-Hcl, 10 mM CaCl2

7.2

25°C

Casein

free amino acids, Peptides

—

Classical aqueous control (in amount of released soluble peptide fragments equivalent to 0.001 A280 per minute at standard condition/% casein)

Activity - Michaelis-Menten

V'm/K'm measured by absorbance spectrophotometry (free aromatic amino acids absorbance measurement, 280 nm) in the presence of organic solvent

Isopropanol

104 mM

1104.0

780.0

amount of released soluble peptide fragments equivalent to 0.001 A280 per minute at standard condition/% casein

50 mM Tris-Hcl, 10 mM CaCl2

7.2

25°C

Casein

free amino acids, Peptides

—

Classical aqueous control (in amount of released soluble peptide fragments equivalent to 0.001 A280 per minute at standard condition/% casein)

Activity - Michaelis-Menten

V'm/K'm measured by absorbance spectrophotometry (free aromatic amino acids absorbance measurement, 280 nm) in the presence of organic solvent

Isopropanol

130 mM

1104.0

732.0

amount of released soluble peptide fragments equivalent to 0.001 A280 per minute at standard condition/% casein

50 mM Tris-Hcl, 10 mM CaCl2

7.2

25°C

Casein

free amino acids, Peptides

—

Classical aqueous control (in amount of released soluble peptide fragments equivalent to 0.001 A280 per minute at standard condition/% casein)

Activity - Michaelis-Menten

V'm/K'm measured by absorbance spectrophotometry (free aromatic amino acids absorbance measurement, 280 nm) in the presence of organic solvent

Dimethylformamide (DMF)

130 mM

1104.0

1024.0

amount of released soluble peptide fragments equivalent to 0.001 A280 per minute at standard condition/% casein

50 mM Tris-Hcl, 10 mM CaCl2

7.2

25°C

Casein

free amino acids, Peptides

—

Classical aqueous control (in amount of released soluble peptide fragments equivalent to 0.001 A280 per minute at standard condition/% casein)

Activity - Michaelis-Menten

V'm/K'm measured by absorbance spectrophotometry (free aromatic amino acids absorbance measurement, 280 nm) in the presence of organic solvent

Dimethylformamide (DMF)

260 mM

1104.0

1031.0

amount of released soluble peptide fragments equivalent to 0.001 A280 per minute at standard condition/% casein

50 mM Tris-Hcl, 10 mM CaCl2

7.2

25°C

Casein

free amino acids, Peptides

—

Classical aqueous control (in amount of released soluble peptide fragments equivalent to 0.001 A280 per minute at standard condition/% casein)

Activity - Michaelis-Menten

V'm/K'm measured by absorbance spectrophotometry (free aromatic amino acids absorbance measurement, 280 nm) in the presence of organic solvent

Dimethylformamide (DMF)

390 mM

1104.0

1015.0

amount of released soluble peptide fragments equivalent to 0.001 A280 per minute at standard condition/% casein

50 mM Tris-Hcl, 10 mM CaCl2

7.2

25°C

Casein

free amino acids, Peptides

—

Classical aqueous control (in amount of released soluble peptide fragments equivalent to 0.001 A280 per minute at standard condition/% casein)

Activity - Michaelis-Menten

V'm/K'm measured by absorbance spectrophotometry (free aromatic amino acids absorbance measurement, 280 nm) in the presence of organic solvent

Dimethylformamide (DMF)

650 mM

1104.0

1000.0

amount of released soluble peptide fragments equivalent to 0.001 A280 per minute at standard condition/% casein

50 mM Tris-Hcl, 10 mM CaCl2

7.2

25°C

Casein

free amino acids, Peptides

—

Classical aqueous control (in amount of released soluble peptide fragments equivalent to 0.001 A280 per minute at standard condition/% casein)

Activity - Michaelis-Menten

V'm/K'm measured by absorbance spectrophotometry (free aromatic amino acids absorbance measurement, 280 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

140 mM

1104.0

1038.0

amount of released soluble peptide fragments equivalent to 0.001 A280 per minute at standard condition/% casein

50 mM Tris-Hcl, 10 mM CaCl2

7.2

25°C

Casein

free amino acids, Peptides

—

Classical aqueous control (in amount of released soluble peptide fragments equivalent to 0.001 A280 per minute at standard condition/% casein)

Activity - Michaelis-Menten

V'm/K'm measured by absorbance spectrophotometry (free aromatic amino acids absorbance measurement, 280 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

280 mM

1104.0

1057.0

amount of released soluble peptide fragments equivalent to 0.001 A280 per minute at standard condition/% casein

50 mM Tris-Hcl, 10 mM CaCl2

7.2

25°C

Casein

free amino acids, Peptides

—

Classical aqueous control (in amount of released soluble peptide fragments equivalent to 0.001 A280 per minute at standard condition/% casein)

Activity - Michaelis-Menten

V'm/K'm measured by absorbance spectrophotometry (free aromatic amino acids absorbance measurement, 280 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

420 mM

1104.0

1014.0

amount of released soluble peptide fragments equivalent to 0.001 A280 per minute at standard condition/% casein

50 mM Tris-Hcl, 10 mM CaCl2

7.2

25°C

Casein

free amino acids, Peptides

—

Classical aqueous control (in amount of released soluble peptide fragments equivalent to 0.001 A280 per minute at standard condition/% casein)

Activity - Michaelis-Menten

V'm/K'm measured by absorbance spectrophotometry (free aromatic amino acids absorbance measurement, 280 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

700 mM

1104.0

1003.0

amount of released soluble peptide fragments equivalent to 0.001 A280 per minute at standard condition/% casein

50 mM Tris-Hcl, 10 mM CaCl2

7.2

25°C

Casein

free amino acids, Peptides

—

Classical aqueous control (in amount of released soluble peptide fragments equivalent to 0.001 A280 per minute at standard condition/% casein)

Activity - Michaelis-Menten

K'm (in % Casein) measured by absorbance spectrophotometry (free aromatic amino acids absorbance measurement, 280 nm) in the presence of organic solvent

Isopropanol

130 mM

0.086

0.097

% casein

50 mM Tris-Hcl, 10 mM CaCl2

7.2

25°C

Casein

free amino acids, Peptides

—

Classical aqueous control (in % casein)

Activity - Michaelis-Menten

V'm (one unit is the amount of released soluble peptides fragments equivalent to 0.001 A280 per minute at standard condition) measured by absorbance spectrophotometry (free aromatic amino acids absorbance measurement, 280 nm) in the presence of organic solvent

1-Propanol

26.6 mM

95.0

87.0

amount of released soluble peptides fragments equivalent to 0.001 A280 per minute at standard condition

50 mM Tris-Hcl, 10 mM CaCl2

7.2

25°C

Casein

free amino acids, Peptides

—

Classical aqueous control (in amount of released soluble peptides fragments equivalent to 0.001 A280 per minute at standard condition)

Activity - Michaelis-Menten

1-Propanol

65.6 mM

95.0

81.0

amount of released soluble peptides fragments equivalent to 0.001 A280 per minute at standard condition

50 mM Tris-Hcl, 10 mM CaCl2

7.2

25°C

Casein

free amino acids, Peptides

—

Classical aqueous control (in amount of released soluble peptides fragments equivalent to 0.001 A280 per minute at standard condition)

Activity - Michaelis-Menten

1-Propanol

106.6 mM

95.0

68.0

amount of released soluble peptides fragments equivalent to 0.001 A280 per minute at standard condition

50 mM Tris-Hcl, 10 mM CaCl2

7.2

25°C

Casein

free amino acids, Peptides

—

Classical aqueous control (in amount of released soluble peptides fragments equivalent to 0.001 A280 per minute at standard condition)

Activity - Michaelis-Menten

1-Propanol

133 mM

95.0

63.0

amount of released soluble peptides fragments equivalent to 0.001 A280 per minute at standard condition

50 mM Tris-Hcl, 10 mM CaCl2

7.2

25°C

Casein

free amino acids, Peptides

—

Classical aqueous control (in amount of released soluble peptides fragments equivalent to 0.001 A280 per minute at standard condition)

Activity - Michaelis-Menten

Isopropanol

26 mM

95.0

84.0

amount of released soluble peptides fragments equivalent to 0.001 A280 per minute at standard condition

50 mM Tris-Hcl, 10 mM CaCl2

7.2

25°C

Casein

free amino acids, Peptides

—

Classical aqueous control (in amount of released soluble peptides fragments equivalent to 0.001 A280 per minute at standard condition)

Activity - Michaelis-Menten

Isopropanol

65 mM

95.0

81.0

amount of released soluble peptides fragments equivalent to 0.001 A280 per minute at standard condition

50 mM Tris-Hcl, 10 mM CaCl2

7.2

25°C

Casein

free amino acids, Peptides

—

Classical aqueous control (in amount of released soluble peptides fragments equivalent to 0.001 A280 per minute at standard condition)

Activity - Michaelis-Menten

Isopropanol

104 mM

95.0

78.0

amount of released soluble peptides fragments equivalent to 0.001 A280 per minute at standard condition

50 mM Tris-Hcl, 10 mM CaCl2

7.2

25°C

Casein

free amino acids, Peptides

—

Classical aqueous control (in amount of released soluble peptides fragments equivalent to 0.001 A280 per minute at standard condition)

Activity - Michaelis-Menten

Isopropanol

130 mM

95.0

71.0

amount of released soluble peptides fragments equivalent to 0.001 A280 per minute at standard condition

50 mM Tris-Hcl, 10 mM CaCl2

7.2

25°C

Casein

free amino acids, Peptides

—

Classical aqueous control (in amount of released soluble peptides fragments equivalent to 0.001 A280 per minute at standard condition)

Activity - Michaelis-Menten

Dimethylformamide (DMF)

130 mM

95.0

84.0

amount of released soluble peptides fragments equivalent to 0.001 A280 per minute at standard condition

50 mM Tris-Hcl, 10 mM CaCl2

7.2

25°C

Casein

free amino acids, Peptides

—

Classical aqueous control (in amount of released soluble peptides fragments equivalent to 0.001 A280 per minute at standard condition)

Activity - Michaelis-Menten

Dimethylformamide (DMF)

260 mM

95.0

66.0

amount of released soluble peptides fragments equivalent to 0.001 A280 per minute at standard condition

50 mM Tris-Hcl, 10 mM CaCl2

7.2

25°C

Casein

free amino acids, Peptides

—

Classical aqueous control (in amount of released soluble peptides fragments equivalent to 0.001 A280 per minute at standard condition)

Activity - Michaelis-Menten

Dimethylformamide (DMF)

390 mM

95.0

65.0

amount of released soluble peptides fragments equivalent to 0.001 A280 per minute at standard condition

50 mM Tris-Hcl, 10 mM CaCl2

7.2

25°C

Casein

free amino acids, Peptides

—

Classical aqueous control (in amount of released soluble peptides fragments equivalent to 0.001 A280 per minute at standard condition)

Activity - Michaelis-Menten

Dimethylformamide (DMF)

650 mM

95.0

56.0

amount of released soluble peptides fragments equivalent to 0.001 A280 per minute at standard condition

50 mM Tris-Hcl, 10 mM CaCl2

7.2

25°C

Casein

free amino acids, Peptides

—

Classical aqueous control (in amount of released soluble peptides fragments equivalent to 0.001 A280 per minute at standard condition)

Activity - Michaelis-Menten

Dimethyl Sulfoxide (DMSO)

140 mM

95.0

80.0

amount of released soluble peptides fragments equivalent to 0.001 A280 per minute at standard condition

50 mM Tris-Hcl, 10 mM CaCl2

7.2

25°C

Casein

free amino acids, Peptides

—

Classical aqueous control (in amount of released soluble peptides fragments equivalent to 0.001 A280 per minute at standard condition)

Activity - Michaelis-Menten

Dimethyl Sulfoxide (DMSO)

280 mM

95.0

75.0

amount of released soluble peptides fragments equivalent to 0.001 A280 per minute at standard condition

50 mM Tris-Hcl, 10 mM CaCl2

7.2

25°C

Casein

free amino acids, Peptides

—

Classical aqueous control (in amount of released soluble peptides fragments equivalent to 0.001 A280 per minute at standard condition)

Activity - Michaelis-Menten

Dimethyl Sulfoxide (DMSO)

420 mM

95.0

72.0

amount of released soluble peptides fragments equivalent to 0.001 A280 per minute at standard condition

50 mM Tris-Hcl, 10 mM CaCl2

7.2

25°C

Casein

free amino acids, Peptides

—

Classical aqueous control (in amount of released soluble peptides fragments equivalent to 0.001 A280 per minute at standard condition)

Activity - Michaelis-Menten

Dimethyl Sulfoxide (DMSO)

700 mM

95.0

67.0

amount of released soluble peptides fragments equivalent to 0.001 A280 per minute at standard condition

50 mM Tris-Hcl, 10 mM CaCl2

7.2

25°C

Casein

free amino acids, Peptides

—

Classical aqueous control (in amount of released soluble peptides fragments equivalent to 0.001 A280 per minute at standard condition)

Activity - Michaelis-Menten

K'm (in % Casein) measured by absorbance spectrophotometry (free aromatic amino acids absorbance measurement, 280 nm) in the presence of organic solvent

1-Propanol

26.6 mM

0.086

0.088

% casein

50 mM Tris-Hcl, 10 mM CaCl2

7.2

25°C

Casein

free amino acids, Peptides

—

Classical aqueous control (in % casein)

Activity - Michaelis-Menten

K'm (in % Casein) measured by absorbance spectrophotometry (free aromatic amino acids absorbance measurement, 280 nm) in the presence of organic solvent

1-Propanol

65.6 mM

0.086

0.093

% casein

50 mM Tris-Hcl, 10 mM CaCl2

7.2

25°C

Casein

free amino acids, Peptides

—

Classical aqueous control (in % casein)

Activity - Michaelis-Menten

K'm (in % Casein) measured by absorbance spectrophotometry (free aromatic amino acids absorbance measurement, 280 nm) in the presence of organic solvent

1-Propanol

133 mM

0.086

0.1

% casein

50 mM Tris-Hcl, 10 mM CaCl2

7.2

25°C

Casein

free amino acids, Peptides

—

Classical aqueous control (in % casein)

Activity - Michaelis-Menten

K'm (in % Casein) measured by absorbance spectrophotometry (free aromatic amino acids absorbance measurement, 280 nm) in the presence of organic solvent

Isopropanol

26 mM

0.086

0.082

% casein

50 mM Tris-Hcl, 10 mM CaCl2

7.2

25°C

Casein

free amino acids, Peptides

—

Classical aqueous control (in % casein)

Activity - Michaelis-Menten

K'm (in % Casein) measured by absorbance spectrophotometry (free aromatic amino acids absorbance measurement, 280 nm) in the presence of organic solvent

Isopropanol

65 mM

0.086

0.091

% casein

50 mM Tris-Hcl, 10 mM CaCl2

7.2

25°C

Casein

free amino acids, Peptides

—

Classical aqueous control (in % casein)

Activity - Michaelis-Menten

K'm (in % Casein) measured by absorbance spectrophotometry (free aromatic amino acids absorbance measurement, 280 nm) in the presence of organic solvent

Isopropanol

104 mM

0.086

0.1

% casein

50 mM Tris-Hcl, 10 mM CaCl2

7.2

25°C

Casein

free amino acids, Peptides

—

Classical aqueous control (in % casein)

Activity - Michaelis-Menten

K'm (in % Casein) measured by absorbance spectrophotometry (free aromatic amino acids absorbance measurement, 280 nm) in the presence of organic solvent

Dimethylformamide (DMF)

130 mM

0.086

0.082

% casein

50 mM Tris-Hcl, 10 mM CaCl2

7.2

25°C

Casein

free amino acids, Peptides

—

Classical aqueous control (in % casein)

Stability - Half-life

Half-life (in minutes) at 80°C, with residual activity measured by absorbance spectrophotometry (free aromatic amino acids absorbance measurement, 280 nm)

1-Propanol

50%

>20.0

3.0

minutes

Incubation: 50 mM Tris-Hcl, 10 mM CaCl2, Assay: 50 mM Tris-Hcl, 10 mM CaCl2

Incubation: 7.2, Assay: 7.2

Incubation: 80°C, Assay: 25°C

0.5% Casein

free amino acids, Peptides

—

Classical aqueous control (in minutes)

Stability - Half-life

Half-life (in minutes) at 80°C, with residual activity measured by absorbance spectrophotometry (free aromatic amino acids absorbance measurement, 280 nm)

Isopropanol

50%

>20.0

4.0

minutes

Incubation: 50 mM Tris-Hcl, 10 mM CaCl2, Assay: 50 mM Tris-Hcl, 10 mM CaCl2

Incubation: 7.2, Assay: 7.2

Incubation: 80°C, Assay: 25°C

0.5% Casein

free amino acids, Peptides

—

Classical aqueous control (in minutes)

Stability - Half-life

Half-life (in minutes) at 80°C, with residual activity measured by absorbance spectrophotometry (free aromatic amino acids absorbance measurement, 280 nm)

Dimethylformamide (DMF)

50%

>20.0

6.0

minutes

Incubation: 50 mM Tris-Hcl, 10 mM CaCl2, Assay: 50 mM Tris-Hcl, 10 mM CaCl2

Incubation: 7.2, Assay: 7.2

Incubation: 80°C, Assay: 25°C

0.5% Casein

free amino acids, Peptides

—

Classical aqueous control (in minutes)

Stability - Half-life

Half-life (in minutes) at 80°C, with residual activity measured by absorbance spectrophotometry (free aromatic amino acids absorbance measurement, 280 nm)

Dimethyl Sulfoxide (DMSO)

50%

>20.0

minutes

Incubation: 50 mM Tris-Hcl, 10 mM CaCl2, Assay: 50 mM Tris-Hcl, 10 mM CaCl2

Incubation: 7.2, Assay: 7.2

Incubation: 80°C, Assay: 25°C

0.5% Casein

free amino acids, Peptides

—

Classical aqueous control (in minutes)

‹ Prev

1 2 3 4

Next ›

Visualization : Activity — Classical

Mohammad Pazhang et al. (2006)

One bar per measurement. Colour = solvent, shade = solvent volume.

Visualization : Activity — Michaelis-Menten

Mohammad Pazhang et al. (2006)

One bar per measurement. Colour = solvent, shade = solvent volume.

Visualization : Stability — Half-life

Mohammad Pazhang et al. (2006)

One bar per measurement. Colour = solvent, shade = solvent volume.

Bassem Jaouadi et al. (2013) — Biochemical and molecular characterization of Pseudomonas aeruginosa CTM50182 organic solvent-stable elastase

International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2013.05.019 ↗ · Stability - Incubation

40 measurements ▼

Database ID

UniProt: P00800 ↗

Sequence Annotation

Inferred - from protein name (232 first residues from UniProt sequence absent from the mature protein)

Protein Source

Commercially purchased

Experimental Data (40 measurements)

40 measurements — page 1 of 2

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Stability - Incubation	Activity after incubation (90 days at 30°C), measured by absorbance spectrophotometry (colorimetric assay, Folin–Ciocalteu reaction product with free tyrsines absorbance measurement, 660 nm) in aqueous phase	1-Hexanol	60%	100	52	%	Incubation: ?, Assay: 50 mM Na2HPO4–NaOH buffer, 5mM CoCl2	Incubation: ?, Assay: 10	Incubation: 30°C, Assay: 60°C	? mM Casein	free amino acids , Peptides	—	Incubation: 150 rpm	Non-incubated control (in %), activity measured in aqueous phase \| High uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (90 days at 30°C), measured by absorbance spectrophotometry (colorimetric assay, Folin–Ciocalteu reaction product with free tyrsines absorbance measurement, 660 nm) in aqueous phase	n-Decane	60%	100	77	%	Incubation: ?, Assay: 50 mM Na2HPO4–NaOH buffer, 5mM CoCl2	Incubation: ?, Assay: 10	Incubation: 30°C, Assay: 60°C	? mM Casein	free amino acids , Peptides	—	Incubation: 150 rpm	Non-incubated control (in %), activity measured in aqueous phase \| High uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (90 days at 30°C), measured by absorbance spectrophotometry (colorimetric assay, Folin–Ciocalteu reaction product with free tyrsines absorbance measurement, 660 nm) in aqueous phase	Isooctane	60%	100	108	%	Incubation: ?, Assay: 50 mM Na2HPO4–NaOH buffer, 5mM CoCl2	Incubation: ?, Assay: 10	Incubation: 30°C, Assay: 60°C	? mM Casein	free amino acids , Peptides	—	Incubation: 150 rpm	Non-incubated control (in %), activity measured in aqueous phase \| High uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (90 days at 30°C), measured by absorbance spectrophotometry (colorimetric assay, Folin–Ciocalteu reaction product with free tyrsines absorbance measurement, 660 nm) in aqueous phase	n-Octane	60%	100	112	%	Incubation: ?, Assay: 50 mM Na2HPO4–NaOH buffer, 5mM CoCl2	Incubation: ?, Assay: 10	Incubation: 30°C, Assay: 60°C	? mM Casein	free amino acids , Peptides	—	Incubation: 150 rpm	Non-incubated control (in %), activity measured in aqueous phase \| High uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (90 days at 30°C), measured by absorbance spectrophotometry (colorimetric assay, Folin–Ciocalteu reaction product with free tyrsines absorbance measurement, 660 nm) in aqueous phase	n-Heptane	60%	100	127	%	Incubation: ?, Assay: 50 mM Na2HPO4–NaOH buffer, 5mM CoCl2	Incubation: ?, Assay: 10	Incubation: 30°C, Assay: 60°C	? mM Casein	free amino acids , Peptides	—	Incubation: 150 rpm	Non-incubated control (in %), activity measured in aqueous phase \| High uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (90 days at 30°C), measured by absorbance spectrophotometry (colorimetric assay, Folin–Ciocalteu reaction product with free tyrsines absorbance measurement, 660 nm) in aqueous phase	n-Hexane	60%	100	135	%	Incubation: ?, Assay: 50 mM Na2HPO4–NaOH buffer, 5mM CoCl2	Incubation: ?, Assay: 10	Incubation: 30°C, Assay: 60°C	? mM Casein	free amino acids , Peptides	—	Incubation: 150 rpm	Non-incubated control (in %), activity measured in aqueous phase \| High uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (90 days at 30°C), measured by absorbance spectrophotometry (colorimetric assay, Folin–Ciocalteu reaction product with free tyrsines absorbance measurement, 660 nm) in aqueous phase	Cyclohexane	60%	100	125	%	Incubation: ?, Assay: 50 mM Na2HPO4–NaOH buffer, 5mM CoCl2	Incubation: ?, Assay: 10	Incubation: 30°C, Assay: 60°C	? mM Casein	free amino acids , Peptides	—	Incubation: 150 rpm	Non-incubated control (in %), activity measured in aqueous phase \| High uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (90 days at 30°C), measured by absorbance spectrophotometry (colorimetric assay, Folin–Ciocalteu reaction product with free tyrsines absorbance measurement, 660 nm) in aqueous phase	Toluene	60%	100	72	%	Incubation: ?, Assay: 50 mM Na2HPO4–NaOH buffer, 5mM CoCl2	Incubation: ?, Assay: 10	Incubation: 30°C, Assay: 60°C	? mM Casein	free amino acids , Peptides	—	Incubation: 150 rpm	Non-incubated control (in %), activity measured in aqueous phase \| High uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (90 days at 30°C), measured by absorbance spectrophotometry (colorimetric assay, Folin–Ciocalteu reaction product with free tyrsines absorbance measurement, 660 nm) in aqueous phase	Benzene	60%	100	60	%	Incubation: ?, Assay: 50 mM Na2HPO4–NaOH buffer, 5mM CoCl2	Incubation: ?, Assay: 10	Incubation: 30°C, Assay: 60°C	? mM Casein	free amino acids , Peptides	—	Incubation: 150 rpm	Non-incubated control (in %), activity measured in aqueous phase \| High uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (90 days at 30°C), measured by absorbance spectrophotometry (colorimetric assay, Folin–Ciocalteu reaction product with free tyrsines absorbance measurement, 660 nm) in aqueous phase	Chloroform	60%	100	83	%	Incubation: ?, Assay: 50 mM Na2HPO4–NaOH buffer, 5mM CoCl2	Incubation: ?, Assay: 10	Incubation: 30°C, Assay: 60°C	? mM Casein	free amino acids , Peptides	—	Incubation: 150 rpm	Non-incubated control (in %), activity measured in aqueous phase \| High uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (90 days at 30°C), measured by absorbance spectrophotometry (colorimetric assay, Folin–Ciocalteu reaction product with free tyrsines absorbance measurement, 660 nm) in aqueous phase	n-Hexadecane	60%	100	95	%	Incubation: ?, Assay: 50 mM Na2HPO4–NaOH buffer, 5mM CoCl2	Incubation: ?, Assay: 10	Incubation: 30°C, Assay: 60°C	? mM Casein	free amino acids , Peptides	—	Incubation: 150 rpm	Non-incubated control (in %), activity measured in aqueous phase \| High uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (90 days at 30°C), measured by absorbance spectrophotometry (colorimetric assay, Folin–Ciocalteu reaction product with free tyrsines absorbance measurement, 660 nm) in aqueous phase	1-Butanol	60%	100	38	%	Incubation: ?, Assay: 50 mM Na2HPO4–NaOH buffer, 5mM CoCl2	Incubation: ?, Assay: 10	Incubation: 30°C, Assay: 60°C	? mM Casein	free amino acids , Peptides	—	Incubation: 150 rpm	Non-incubated control (in %), activity measured in aqueous phase \| High uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (90 days at 30°C), measured by absorbance spectrophotometry (colorimetric assay, Folin–Ciocalteu reaction product with free tyrsines absorbance measurement, 660 nm) in aqueous phase	Ethyl Acetate	60%	100	0	%	Incubation: ?, Assay: 50 mM Na2HPO4–NaOH buffer, 5mM CoCl2	Incubation: ?, Assay: 10	Incubation: 30°C, Assay: 60°C	? mM Casein	free amino acids , Peptides	—	Incubation: 150 rpm	Non-incubated control (in %), activity measured in aqueous phase \| High uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (90 days at 30°C), measured by absorbance spectrophotometry (colorimetric assay, Folin–Ciocalteu reaction product with free tyrsines absorbance measurement, 660 nm) in aqueous phase	Isopropanol	60%	100	91	%	Incubation: ?, Assay: 50 mM Na2HPO4–NaOH buffer, 5mM CoCl2	Incubation: ?, Assay: 10	Incubation: 30°C, Assay: 60°C	? mM Casein	free amino acids , Peptides	—	Incubation: 150 rpm	Non-incubated control (in %), activity measured in aqueous phase \| High uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (90 days at 30°C), measured by absorbance spectrophotometry (colorimetric assay, Folin–Ciocalteu reaction product with free tyrsines absorbance measurement, 660 nm) in aqueous phase	Isopropanol	60%	100	88	%	Incubation: ?, Assay: 50 mM Na2HPO4–NaOH buffer, 5mM CoCl2	Incubation: ?, Assay: 10	Incubation: 30°C, Assay: 60°C	? mM Casein	free amino acids , Peptides	—	Incubation: 150 rpm	Non-incubated control (in %), activity measured in aqueous phase \| High uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (90 days at 30°C), measured by absorbance spectrophotometry (colorimetric assay, Folin–Ciocalteu reaction product with free tyrsines absorbance measurement, 660 nm) in aqueous phase	Acetonitrile	60%	100	0	%	Incubation: ?, Assay: 50 mM Na2HPO4–NaOH buffer, 5mM CoCl2	Incubation: ?, Assay: 10	Incubation: 30°C, Assay: 60°C	? mM Casein	free amino acids , Peptides	—	Incubation: 150 rpm	Non-incubated control (in %), activity measured in aqueous phase \| High uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (90 days at 30°C), measured by absorbance spectrophotometry (colorimetric assay, Folin–Ciocalteu reaction product with free tyrsines absorbance measurement, 660 nm) in aqueous phase	Ethanol	60%	100	71	%	Incubation: ?, Assay: 50 mM Na2HPO4–NaOH buffer, 5mM CoCl2	Incubation: ?, Assay: 10	Incubation: 30°C, Assay: 60°C	? mM Casein	free amino acids , Peptides	—	Incubation: 150 rpm	Non-incubated control (in %), activity measured in aqueous phase \| High uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (90 days at 30°C), measured by absorbance spectrophotometry (colorimetric assay, Folin–Ciocalteu reaction product with free tyrsines absorbance measurement, 660 nm) in aqueous phase	Methanol	60%	100	75	%	Incubation: ?, Assay: 50 mM Na2HPO4–NaOH buffer, 5mM CoCl2	Incubation: ?, Assay: 10	Incubation: 30°C, Assay: 60°C	? mM Casein	free amino acids , Peptides	—	Incubation: 150 rpm	Non-incubated control (in %), activity measured in aqueous phase \| High uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (90 days at 30°C), measured by absorbance spectrophotometry (colorimetric assay, Folin–Ciocalteu reaction product with free tyrsines absorbance measurement, 660 nm) in aqueous phase	Dimethylformamide (DMF)	60%	100	115	%	Incubation: ?, Assay: 50 mM Na2HPO4–NaOH buffer, 5mM CoCl2	Incubation: ?, Assay: 10	Incubation: 30°C, Assay: 60°C	? mM Casein	free amino acids , Peptides	—	Incubation: 150 rpm	Non-incubated control (in %), activity measured in aqueous phase \| High uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (90 days at 30°C), measured by absorbance spectrophotometry (colorimetric assay, Folin–Ciocalteu reaction product with free tyrsines absorbance measurement, 660 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	60%	100	127	%	Incubation: ?, Assay: 50 mM Na2HPO4–NaOH buffer, 5mM CoCl2	Incubation: ?, Assay: 10	Incubation: 30°C, Assay: 60°C	? mM Casein	free amino acids , Peptides	—	Incubation: 150 rpm	Non-incubated control (in %), activity measured in aqueous phase \| High uncertainty about whether assay in aqueous phase or in the presence of organic solvent

Stability - Incubation

Activity after incubation (90 days at 30°C), measured by absorbance spectrophotometry (colorimetric assay, Folin–Ciocalteu reaction product with free tyrsines absorbance measurement, 660 nm) in aqueous phase

1-Hexanol

60%

100

Incubation: ?, Assay: 50 mM Na2HPO4–NaOH buffer, 5mM CoCl2

Incubation: ?, Assay: 10

Incubation: 30°C, Assay: 60°C

? mM Casein

free amino acids, Peptides

—

Incubation: 150 rpm

Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent

Stability - Incubation

n-Decane

60%

100

Incubation: ?, Assay: 50 mM Na2HPO4–NaOH buffer, 5mM CoCl2

Incubation: ?, Assay: 10

Incubation: 30°C, Assay: 60°C

? mM Casein

free amino acids, Peptides

—

Incubation: 150 rpm

Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent

Stability - Incubation

Isooctane

60%

100

108

Incubation: ?, Assay: 50 mM Na2HPO4–NaOH buffer, 5mM CoCl2

Incubation: ?, Assay: 10

Incubation: 30°C, Assay: 60°C

? mM Casein

free amino acids, Peptides

—

Incubation: 150 rpm

Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent

Stability - Incubation

n-Octane

60%

100

112

Incubation: ?, Assay: 50 mM Na2HPO4–NaOH buffer, 5mM CoCl2

Incubation: ?, Assay: 10

Incubation: 30°C, Assay: 60°C

? mM Casein

free amino acids, Peptides

—

Incubation: 150 rpm

Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent

Stability - Incubation

n-Heptane

60%

100

127

Incubation: ?, Assay: 50 mM Na2HPO4–NaOH buffer, 5mM CoCl2

Incubation: ?, Assay: 10

Incubation: 30°C, Assay: 60°C

? mM Casein

free amino acids, Peptides

—

Incubation: 150 rpm

Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent

Stability - Incubation

n-Hexane

60%

100

135

Incubation: ?, Assay: 50 mM Na2HPO4–NaOH buffer, 5mM CoCl2

Incubation: ?, Assay: 10

Incubation: 30°C, Assay: 60°C

? mM Casein

free amino acids, Peptides

—

Incubation: 150 rpm

Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent

Stability - Incubation

Cyclohexane

60%

100

125

Incubation: ?, Assay: 50 mM Na2HPO4–NaOH buffer, 5mM CoCl2

Incubation: ?, Assay: 10

Incubation: 30°C, Assay: 60°C

? mM Casein

free amino acids, Peptides

—

Incubation: 150 rpm

Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent

Stability - Incubation

Toluene

60%

100

Incubation: ?, Assay: 50 mM Na2HPO4–NaOH buffer, 5mM CoCl2

Incubation: ?, Assay: 10

Incubation: 30°C, Assay: 60°C

? mM Casein

free amino acids, Peptides

—

Incubation: 150 rpm

Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent

Stability - Incubation

Benzene

60%

100

Incubation: ?, Assay: 50 mM Na2HPO4–NaOH buffer, 5mM CoCl2

Incubation: ?, Assay: 10

Incubation: 30°C, Assay: 60°C

? mM Casein

free amino acids, Peptides

—

Incubation: 150 rpm

Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent

Stability - Incubation

Chloroform

60%

100

Incubation: ?, Assay: 50 mM Na2HPO4–NaOH buffer, 5mM CoCl2

Incubation: ?, Assay: 10

Incubation: 30°C, Assay: 60°C

? mM Casein

free amino acids, Peptides

—

Incubation: 150 rpm

Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent

Stability - Incubation

n-Hexadecane

60%

100

Incubation: ?, Assay: 50 mM Na2HPO4–NaOH buffer, 5mM CoCl2

Incubation: ?, Assay: 10

Incubation: 30°C, Assay: 60°C

? mM Casein

free amino acids, Peptides

—

Incubation: 150 rpm

Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent

Stability - Incubation

1-Butanol

60%

100

Incubation: ?, Assay: 50 mM Na2HPO4–NaOH buffer, 5mM CoCl2

Incubation: ?, Assay: 10

Incubation: 30°C, Assay: 60°C

? mM Casein

free amino acids, Peptides

—

Incubation: 150 rpm

Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent

Stability - Incubation

Ethyl Acetate

60%

100

Incubation: ?, Assay: 50 mM Na2HPO4–NaOH buffer, 5mM CoCl2

Incubation: ?, Assay: 10

Incubation: 30°C, Assay: 60°C

? mM Casein

free amino acids, Peptides

—

Incubation: 150 rpm

Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent

Stability - Incubation

Isopropanol

60%

100

Incubation: ?, Assay: 50 mM Na2HPO4–NaOH buffer, 5mM CoCl2

Incubation: ?, Assay: 10

Incubation: 30°C, Assay: 60°C

? mM Casein

free amino acids, Peptides

—

Incubation: 150 rpm

Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent

Stability - Incubation

Isopropanol

60%

100

Incubation: ?, Assay: 50 mM Na2HPO4–NaOH buffer, 5mM CoCl2

Incubation: ?, Assay: 10

Incubation: 30°C, Assay: 60°C

? mM Casein

free amino acids, Peptides

—

Incubation: 150 rpm

Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent

Stability - Incubation

Acetonitrile

60%

100

Incubation: ?, Assay: 50 mM Na2HPO4–NaOH buffer, 5mM CoCl2

Incubation: ?, Assay: 10

Incubation: 30°C, Assay: 60°C

? mM Casein

free amino acids, Peptides

—

Incubation: 150 rpm

Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent

Stability - Incubation

Ethanol

60%

100

Incubation: ?, Assay: 50 mM Na2HPO4–NaOH buffer, 5mM CoCl2

Incubation: ?, Assay: 10

Incubation: 30°C, Assay: 60°C

? mM Casein

free amino acids, Peptides

—

Incubation: 150 rpm

Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent

Stability - Incubation

Methanol

60%

100

Incubation: ?, Assay: 50 mM Na2HPO4–NaOH buffer, 5mM CoCl2

Incubation: ?, Assay: 10

Incubation: 30°C, Assay: 60°C

? mM Casein

free amino acids, Peptides

—

Incubation: 150 rpm

Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent

Stability - Incubation

Dimethylformamide (DMF)

60%

100

115

Incubation: ?, Assay: 50 mM Na2HPO4–NaOH buffer, 5mM CoCl2

Incubation: ?, Assay: 10

Incubation: 30°C, Assay: 60°C

? mM Casein

free amino acids, Peptides

—

Incubation: 150 rpm

Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent

Stability - Incubation

Dimethyl Sulfoxide (DMSO)

60%

100

127

Incubation: ?, Assay: 50 mM Na2HPO4–NaOH buffer, 5mM CoCl2

Incubation: ?, Assay: 10

Incubation: 30°C, Assay: 60°C

? mM Casein

free amino acids, Peptides

—

Incubation: 150 rpm

Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent

Stability - Incubation

Activity after incubation (1 day at 30°C), measured by absorbance spectrophotometry (colorimetric assay, Folin–Ciocalteu reaction product with free tyrsines absorbance measurement, 660 nm) in aqueous phase

1-Hexanol

60%

100

Incubation: ?, Assay: 50 mM Na2HPO4–NaOH buffer, 5mM CoCl2

Incubation: ?, Assay: 10

Incubation: 30°C, Assay: 60°C

? mM Casein

free amino acids, Peptides

—

Incubation: 150 rpm

Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent

Stability - Incubation

n-Decane

60%

100

Incubation: ?, Assay: 50 mM Na2HPO4–NaOH buffer, 5mM CoCl2

Incubation: ?, Assay: 10

Incubation: 30°C, Assay: 60°C

? mM Casein

free amino acids, Peptides

—

Incubation: 150 rpm

Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent

Stability - Incubation

Isooctane

60%

100

120

Incubation: ?, Assay: 50 mM Na2HPO4–NaOH buffer, 5mM CoCl2

Incubation: ?, Assay: 10

Incubation: 30°C, Assay: 60°C

? mM Casein

free amino acids, Peptides

—

Incubation: 150 rpm

Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent

Stability - Incubation

n-Octane

60%

100

133

Incubation: ?, Assay: 50 mM Na2HPO4–NaOH buffer, 5mM CoCl2

Incubation: ?, Assay: 10

Incubation: 30°C, Assay: 60°C

? mM Casein

free amino acids, Peptides

—

Incubation: 150 rpm

Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent

Stability - Incubation

n-Heptane

60%

100

140

Incubation: ?, Assay: 50 mM Na2HPO4–NaOH buffer, 5mM CoCl2

Incubation: ?, Assay: 10

Incubation: 30°C, Assay: 60°C

? mM Casein

free amino acids, Peptides

—

Incubation: 150 rpm

Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent

Stability - Incubation

n-Hexane

60%

100

150

Incubation: ?, Assay: 50 mM Na2HPO4–NaOH buffer, 5mM CoCl2

Incubation: ?, Assay: 10

Incubation: 30°C, Assay: 60°C

? mM Casein

free amino acids, Peptides

—

Incubation: 150 rpm

Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent

Stability - Incubation

Cyclohexane

60%

100

138

Incubation: ?, Assay: 50 mM Na2HPO4–NaOH buffer, 5mM CoCl2

Incubation: ?, Assay: 10

Incubation: 30°C, Assay: 60°C

? mM Casein

free amino acids, Peptides

—

Incubation: 150 rpm

Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent

Stability - Incubation

Toluene

60%

100

Incubation: ?, Assay: 50 mM Na2HPO4–NaOH buffer, 5mM CoCl2

Incubation: ?, Assay: 10

Incubation: 30°C, Assay: 60°C

? mM Casein

free amino acids, Peptides

—

Incubation: 150 rpm

Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent

Stability - Incubation

Benzene

60%

100

Incubation: ?, Assay: 50 mM Na2HPO4–NaOH buffer, 5mM CoCl2

Incubation: ?, Assay: 10

Incubation: 30°C, Assay: 60°C

? mM Casein

free amino acids, Peptides

—

Incubation: 150 rpm

Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent

Stability - Incubation

Chloroform

60%

100

103

Incubation: ?, Assay: 50 mM Na2HPO4–NaOH buffer, 5mM CoCl2

Incubation: ?, Assay: 10

Incubation: 30°C, Assay: 60°C

? mM Casein

free amino acids, Peptides

—

Incubation: 150 rpm

Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent

Stability - Incubation

n-Hexadecane

60%

100

105

Incubation: ?, Assay: 50 mM Na2HPO4–NaOH buffer, 5mM CoCl2

Incubation: ?, Assay: 10

Incubation: 30°C, Assay: 60°C

? mM Casein

free amino acids, Peptides

—

Incubation: 150 rpm

Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent

Stability - Incubation

1-Butanol

60%

100

Incubation: ?, Assay: 50 mM Na2HPO4–NaOH buffer, 5mM CoCl2

Incubation: ?, Assay: 10

Incubation: 30°C, Assay: 60°C

? mM Casein

free amino acids, Peptides

—

Incubation: 150 rpm

Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent

Stability - Incubation

Ethyl Acetate

60%

100

Incubation: ?, Assay: 50 mM Na2HPO4–NaOH buffer, 5mM CoCl2

Incubation: ?, Assay: 10

Incubation: 30°C, Assay: 60°C

? mM Casein

free amino acids, Peptides

—

Incubation: 150 rpm

Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent

Stability - Incubation

Isopropanol

60%

100

101

Incubation: ?, Assay: 50 mM Na2HPO4–NaOH buffer, 5mM CoCl2

Incubation: ?, Assay: 10

Incubation: 30°C, Assay: 60°C

? mM Casein

free amino acids, Peptides

—

Incubation: 150 rpm

Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent

Stability - Incubation

Isopropanol

60%

100

102

Incubation: ?, Assay: 50 mM Na2HPO4–NaOH buffer, 5mM CoCl2

Incubation: ?, Assay: 10

Incubation: 30°C, Assay: 60°C

? mM Casein

free amino acids, Peptides

—

Incubation: 150 rpm

Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent

Stability - Incubation

Acetonitrile

60%

100

Incubation: ?, Assay: 50 mM Na2HPO4–NaOH buffer, 5mM CoCl2

Incubation: ?, Assay: 10

Incubation: 30°C, Assay: 60°C

? mM Casein

free amino acids, Peptides

—

Incubation: 150 rpm

Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent

Stability - Incubation

Ethanol

60%

100

Incubation: ?, Assay: 50 mM Na2HPO4–NaOH buffer, 5mM CoCl2

Incubation: ?, Assay: 10

Incubation: 30°C, Assay: 60°C

? mM Casein

free amino acids, Peptides

—

Incubation: 150 rpm

Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent

Stability - Incubation

Methanol

60%

100

Incubation: ?, Assay: 50 mM Na2HPO4–NaOH buffer, 5mM CoCl2

Incubation: ?, Assay: 10

Incubation: 30°C, Assay: 60°C

? mM Casein

free amino acids, Peptides

—

Incubation: 150 rpm

Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent

Stability - Incubation

Dimethylformamide (DMF)

60%

100

125

Incubation: ?, Assay: 50 mM Na2HPO4–NaOH buffer, 5mM CoCl2

Incubation: ?, Assay: 10

Incubation: 30°C, Assay: 60°C

? mM Casein

free amino acids, Peptides

—

Incubation: 150 rpm

Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent

Stability - Incubation

Dimethyl Sulfoxide (DMSO)

60%

100

155

Incubation: ?, Assay: 50 mM Na2HPO4–NaOH buffer, 5mM CoCl2

Incubation: ?, Assay: 10

Incubation: 30°C, Assay: 60°C

? mM Casein

free amino acids, Peptides

—

Incubation: 150 rpm

Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent

‹ Prev

1 2

Next ›

Visualization : Stability — Incubation

Bassem Jaouadi et al. (2013)

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

Drag to rotate · Scroll to zoom · Right-click drag to pan · Powered by Mol*

Thermolysin from Bacillus thermoproteolyticus

Enzyme Description

Sequence

Experimental Data (22 measurements)

Visualization : Activity — Classical

Experimental Data (3 measurements)

Visualization : Activity — Michaelis-Menten

Experimental Data (68 measurements)

Visualization : Activity — Classical

Visualization : Activity — Michaelis-Menten

Visualization : Stability — Half-life

Experimental Data (40 measurements)

Visualization : Stability — Incubation

Structure

Loading…