Thermolysin from Bacillus thermoproteolyticus
Enzyme Description
Species
Extremophile
Yes
"thermophilic" organism
EC Number
Sequence
ITGTSTVGVGRGVLGDQKNINTTYSTYYYLQDNTRGNGIFTYDAKYRTTLPGSLWADADNQFFASYDAPAVDAHYYAGVTYDYYKNVHNRLSYDGNNAAIRSSVHYSQGYNNAFWNGSQMVYGDGDGQTFIPLSGGIDVVAHELTHAVTDYTAGLIYQNESGAINEAISDIFGTLVEFYANKNPDWEIGEDVYTPGISGDSLRSMSDPAKYGDPDHYSKRYTGTQDNGGVHINSGIINKAAYLISQGGTHYGVSVVGIGRDKLGKIFYRALTQYLTPTSNFSQLRAAAVQSATDLYGSTSQEVASVKQAFDAVGVK
Kohei Oda et al. (1996)
β A Novel Alcohol Resistant Metalloproteinase, Vimelysin, from Vibrio sp. T1800: Purification and Characterization
Bioscience, Biotechnology, and Biochemistry
Β· doi:10.1271/bbb.60.463 β
Β· Activity - Classical
▼
Database ID
UniProt: P00800 β
Sequence Annotation
Inferred - from protein name (first 232 residues from UniProt sequence absent from the mature protein)
Protein Source
Commercially purchased
Experimental Data (22 measurements)
22 measurements β page 2 of 2
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Activity - Classical | Activity measured by absorbance spectrophotometry (FAGLA absorbance measurement, 345 nm) in the presence of organic solvent | Methanol | 30% | 100 | 6 | % | 0.1 M MES | 6.5 | ? | 1 mM FAGLA (FA-Gly-DL-Leu-NH2) | Furylacryloyl-Gly , Leu-NH2 | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (FAGLA absorbance measurement, 345 nm) in the presence of organic solvent | Methanol | 20% | 100 | 12 | % | 0.1 M MES | 6.5 | ? | 1 mM FAGLA (FA-Gly-DL-Leu-NH2) | Furylacryloyl-Gly , Leu-NH2 | β | β | Classical aqueous control (in %) |
Visualization : Activity β Classical
Kohei Oda et al. (1996)
One bar per measurement. Colour = solvent, shade = solvent volume.
Guillermo R. Castro et al. (1999)
β Enzymatic activities of proteases dissolved in organic solvents
Enzyme and Microbial Technology
Β· doi:10.1016/S0141-0229(99)00099-X β
Β· Activity - Michaelis-Menten
▼
Database ID
UniProt: P00800 β
Sequence Annotation
Inferred - from protein name (first 232 residues from UniProt sequence absent from the mature protein)
Protein Source
Commercially purchased
Experimental Data (3 measurements)
3 measurements
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Activity - Michaelis-Menten | Km (mM) measured by HPLC in the presence of organic solvent | Glycerol | 99% | 2.5 | 41.4 | mM | 20 mM phosphate buffer | 7.8 | 37Β°C | Phenyl acetate | Acetic Acid , Phenol | β | β | Classical aqueous control (in mM) |
| Activity - Michaelis-Menten | Vmax (Β΅M/s mg) measured by HPLC in the presence of organic solvent | Glycerol | 99% | 0.5 | 0.48 | Β΅M/(s * mg) | 20 mM phosphate buffer | 7.8 | 37Β°C | Phenyl acetate | Acetic Acid , Phenol | β | β | Classical aqueous control (in microM/(s * mg)) |
| Activity - Michaelis-Menten | Vm/Km (1/s mg) measured by HPLC in the presence of organic solvent | Glycerol | 99% | 0.2 | 0.012 | 1/(s * mg) | 20 mM phosphate buffer | 7.8 | 37Β°C | Phenyl acetate | Acetic Acid , Phenol | β | β | Classical aqueous control (in 1/(s * mg)) |
Visualization : Activity β Michaelis-Menten
Guillermo R. Castro et al. (1999)
One bar per measurement. Colour = solvent, shade = solvent volume.
Mohammad Pazhang et al. (2006)
β Effects of water-miscible solvents and polyhydroxy compounds on the structure and enzymatic activity of thermolysin
Journal of Biotechnology
Β· doi:10.1016/j.jbiotec.2006.05.017 β
Β· Activity - Classical Activity - Michaelis-Menten Stability - Half-life
▼
Database ID
UniProt: P00800 β
Sequence Annotation
Inferred - from protein name (first 232 residues from UniProt sequence absent from the mature protein)
Protein Source
Commercially purchased
Experimental Data (68 measurements)
68 measurements β page 1 of 4
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Activity - Classical | Activity measured by absorbance spectrophotometry (free aromatic amino acids absorbance measurement, 280 nm) in the presence of organic solvent | 1-Propanol | 5% | 100.0 | 23.0 | % | 50 mM Tris-Hcl, 10 mM CaCl2 | 7.2 | 25Β°C | 0.5% Casein | free amino acids , Peptides | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (free aromatic amino acids absorbance measurement, 280 nm) in the presence of organic solvent | 1-Propanol | 1% | 100.0 | 81.0 | % | 50 mM Tris-Hcl, 10 mM CaCl2 | 7.2 | 25Β°C | 0.5% Casein | free amino acids , Peptides | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (free aromatic amino acids absorbance measurement, 280 nm) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 20% | 100.0 | 20.0 | % | 50 mM Tris-Hcl, 10 mM CaCl2 | 7.2 | 25Β°C | 0.5% Casein | free amino acids , Peptides | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (free aromatic amino acids absorbance measurement, 280 nm) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 10% | 100.0 | 49.0 | % | 50 mM Tris-Hcl, 10 mM CaCl2 | 7.2 | 25Β°C | 0.5% Casein | free amino acids , Peptides | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (free aromatic amino acids absorbance measurement, 280 nm) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 5% | 100.0 | 69.0 | % | 50 mM Tris-Hcl, 10 mM CaCl2 | 7.2 | 25Β°C | 0.5% Casein | free amino acids , Peptides | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (free aromatic amino acids absorbance measurement, 280 nm) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 1% | 100.0 | 89.0 | % | 50 mM Tris-Hcl, 10 mM CaCl2 | 7.2 | 25Β°C | 0.5% Casein | free amino acids , Peptides | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (free aromatic amino acids absorbance measurement, 280 nm) in the presence of organic solvent | Dimethylformamide (DMF) | 20% | 100.0 | 17.0 | % | 50 mM Tris-Hcl, 10 mM CaCl2 | 7.2 | 25Β°C | 0.5% Casein | free amino acids , Peptides | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (free aromatic amino acids absorbance measurement, 280 nm) in the presence of organic solvent | Dimethylformamide (DMF) | 10% | 100.0 | 43.0 | % | 50 mM Tris-Hcl, 10 mM CaCl2 | 7.2 | 25Β°C | 0.5% Casein | free amino acids , Peptides | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (free aromatic amino acids absorbance measurement, 280 nm) in the presence of organic solvent | Dimethylformamide (DMF) | 5% | 100.0 | 65.0 | % | 50 mM Tris-Hcl, 10 mM CaCl2 | 7.2 | 25Β°C | 0.5% Casein | free amino acids , Peptides | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (free aromatic amino acids absorbance measurement, 280 nm) in the presence of organic solvent | Isopropanol | 20% | 100.0 | 1.0 | % | 50 mM Tris-Hcl, 10 mM CaCl2 | 7.2 | 25Β°C | 0.5% Casein | free amino acids , Peptides | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (free aromatic amino acids absorbance measurement, 280 nm) in the presence of organic solvent | Isopropanol | 10% | 100.0 | 7.0 | % | 50 mM Tris-Hcl, 10 mM CaCl2 | 7.2 | 25Β°C | 0.5% Casein | free amino acids , Peptides | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (free aromatic amino acids absorbance measurement, 280 nm) in the presence of organic solvent | Isopropanol | 5% | 100.0 | 22.0 | % | 50 mM Tris-Hcl, 10 mM CaCl2 | 7.2 | 25Β°C | 0.5% Casein | free amino acids , Peptides | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (free aromatic amino acids absorbance measurement, 280 nm) in the presence of organic solvent | Isopropanol | 1% | 100.0 | 65.0 | % | 50 mM Tris-Hcl, 10 mM CaCl2 | 7.2 | 25Β°C | 0.5% Casein | free amino acids , Peptides | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (free aromatic amino acids absorbance measurement, 280 nm) in the presence of organic solvent | 1-Propanol | 20% | 100.0 | 1.0 | % | 50 mM Tris-Hcl, 10 mM CaCl2 | 7.2 | 25Β°C | 0.5% Casein | free amino acids , Peptides | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (free aromatic amino acids absorbance measurement, 280 nm) in the presence of organic solvent | 1-Propanol | 10% | 100.0 | 3.0 | % | 50 mM Tris-Hcl, 10 mM CaCl2 | 7.2 | 25Β°C | 0.5% Casein | free amino acids , Peptides | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (free aromatic amino acids absorbance measurement, 280 nm) in the presence of organic solvent | Dimethylformamide (DMF) | 1% | 100.0 | 90.0 | % | 50 mM Tris-Hcl, 10 mM CaCl2 | 7.2 | 25Β°C | 0.5% Casein | free amino acids , Peptides | β | β | Classical aqueous control (in %) |
| Activity - Michaelis-Menten | V'm/K'm measured by absorbance spectrophotometry (free aromatic amino acids absorbance measurement, 280 nm) in the presence of organic solvent | 1-Propanol | 133 mM | 1104.0 | 630.0 | amount of released soluble peptide fragments equivalent to 0.001 A280 per minute at standard condition/% casein | 50 mM Tris-Hcl, 10 mM CaCl2 | 7.2 | 25Β°C | Casein | free amino acids , Peptides | β | β | Classical aqueous control (in amount of released soluble peptide fragments equivalent to 0.001 A280 per minute at standard condition/% casein) |
| Activity - Michaelis-Menten | V'm/K'm measured by absorbance spectrophotometry (free aromatic amino acids absorbance measurement, 280 nm) in the presence of organic solvent | 1-Propanol | 106.6 mM | 1104.0 | 747.0 | amount of released soluble peptide fragments equivalent to 0.001 A280 per minute at standard condition/% casein | 50 mM Tris-Hcl, 10 mM CaCl2 | 7.2 | 25Β°C | Casein | free amino acids , Peptides | β | β | Classical aqueous control (in amount of released soluble peptide fragments equivalent to 0.001 A280 per minute at standard condition/% casein) |
| Activity - Michaelis-Menten | V'm/K'm measured by absorbance spectrophotometry (free aromatic amino acids absorbance measurement, 280 nm) in the presence of organic solvent | 1-Propanol | 65.6 mM | 1104.0 | 870.0 | amount of released soluble peptide fragments equivalent to 0.001 A280 per minute at standard condition/% casein | 50 mM Tris-Hcl, 10 mM CaCl2 | 7.2 | 25Β°C | Casein | free amino acids , Peptides | β | β | Classical aqueous control (in amount of released soluble peptide fragments equivalent to 0.001 A280 per minute at standard condition/% casein) |
| Activity - Michaelis-Menten | V'm/K'm measured by absorbance spectrophotometry (free aromatic amino acids absorbance measurement, 280 nm) in the presence of organic solvent | 1-Propanol | 26.6 mM | 1104.0 | 988.0 | amount of released soluble peptide fragments equivalent to 0.001 A280 per minute at standard condition/% casein | 50 mM Tris-Hcl, 10 mM CaCl2 | 7.2 | 25Β°C | Casein | free amino acids , Peptides | β | β | Classical aqueous control (in amount of released soluble peptide fragments equivalent to 0.001 A280 per minute at standard condition/% casein) |
Visualization : Activity β Classical
Mohammad Pazhang et al. (2006)
One bar per measurement. Colour = solvent, shade = solvent volume.
Visualization : Activity β Michaelis-Menten
Mohammad Pazhang et al. (2006)
One bar per measurement. Colour = solvent, shade = solvent volume.
Visualization : Stability β Half-life
Mohammad Pazhang et al. (2006)
One bar per measurement. Colour = solvent, shade = solvent volume.
Bassem Jaouadi et al. (2013)
β Biochemical and molecular characterization of Pseudomonas aeruginosa CTM50182 organic solvent-stable elastase
International Journal of Biological Macromolecules
Β· doi:10.1016/j.ijbiomac.2013.05.019 β
Β· Stability - Incubation
▼
Database ID
UniProt: P00800 β
Sequence Annotation
Inferred - from protein name (232 first residues from UniProt sequence absent from the mature protein)
Protein Source
Commercially purchased
Experimental Data (40 measurements)
40 measurements β page 1 of 2
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Stability - Incubation | Activity after incubation (90 days at 30Β°C), measured by absorbance spectrophotometry (colorimetric assay, FolinβCiocalteu reaction product with free tyrsines absorbance measurement, 660 nm) in aqueous phase | 1-Hexanol | 60% | 100 | 52 | % | Incubation: ?, Assay: 50 mM Na2HPO4βNaOH buffer, 5mM CoCl2 | Incubation: ?, Assay: 10 | Incubation: 30Β°C, Assay: 60Β°C | ? mM Casein | free amino acids , Peptides | β | Incubation: 150 rpm | Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (90 days at 30Β°C), measured by absorbance spectrophotometry (colorimetric assay, FolinβCiocalteu reaction product with free tyrsines absorbance measurement, 660 nm) in aqueous phase | n-Decane | 60% | 100 | 77 | % | Incubation: ?, Assay: 50 mM Na2HPO4βNaOH buffer, 5mM CoCl2 | Incubation: ?, Assay: 10 | Incubation: 30Β°C, Assay: 60Β°C | ? mM Casein | free amino acids , Peptides | β | Incubation: 150 rpm | Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (90 days at 30Β°C), measured by absorbance spectrophotometry (colorimetric assay, FolinβCiocalteu reaction product with free tyrsines absorbance measurement, 660 nm) in aqueous phase | Isooctane | 60% | 100 | 108 | % | Incubation: ?, Assay: 50 mM Na2HPO4βNaOH buffer, 5mM CoCl2 | Incubation: ?, Assay: 10 | Incubation: 30Β°C, Assay: 60Β°C | ? mM Casein | free amino acids , Peptides | β | Incubation: 150 rpm | Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (90 days at 30Β°C), measured by absorbance spectrophotometry (colorimetric assay, FolinβCiocalteu reaction product with free tyrsines absorbance measurement, 660 nm) in aqueous phase | n-Octane | 60% | 100 | 112 | % | Incubation: ?, Assay: 50 mM Na2HPO4βNaOH buffer, 5mM CoCl2 | Incubation: ?, Assay: 10 | Incubation: 30Β°C, Assay: 60Β°C | ? mM Casein | free amino acids , Peptides | β | Incubation: 150 rpm | Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (90 days at 30Β°C), measured by absorbance spectrophotometry (colorimetric assay, FolinβCiocalteu reaction product with free tyrsines absorbance measurement, 660 nm) in aqueous phase | n-Heptane | 60% | 100 | 127 | % | Incubation: ?, Assay: 50 mM Na2HPO4βNaOH buffer, 5mM CoCl2 | Incubation: ?, Assay: 10 | Incubation: 30Β°C, Assay: 60Β°C | ? mM Casein | free amino acids , Peptides | β | Incubation: 150 rpm | Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (90 days at 30Β°C), measured by absorbance spectrophotometry (colorimetric assay, FolinβCiocalteu reaction product with free tyrsines absorbance measurement, 660 nm) in aqueous phase | n-Hexane | 60% | 100 | 135 | % | Incubation: ?, Assay: 50 mM Na2HPO4βNaOH buffer, 5mM CoCl2 | Incubation: ?, Assay: 10 | Incubation: 30Β°C, Assay: 60Β°C | ? mM Casein | free amino acids , Peptides | β | Incubation: 150 rpm | Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (90 days at 30Β°C), measured by absorbance spectrophotometry (colorimetric assay, FolinβCiocalteu reaction product with free tyrsines absorbance measurement, 660 nm) in aqueous phase | Cyclohexane | 60% | 100 | 125 | % | Incubation: ?, Assay: 50 mM Na2HPO4βNaOH buffer, 5mM CoCl2 | Incubation: ?, Assay: 10 | Incubation: 30Β°C, Assay: 60Β°C | ? mM Casein | free amino acids , Peptides | β | Incubation: 150 rpm | Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (90 days at 30Β°C), measured by absorbance spectrophotometry (colorimetric assay, FolinβCiocalteu reaction product with free tyrsines absorbance measurement, 660 nm) in aqueous phase | Toluene | 60% | 100 | 72 | % | Incubation: ?, Assay: 50 mM Na2HPO4βNaOH buffer, 5mM CoCl2 | Incubation: ?, Assay: 10 | Incubation: 30Β°C, Assay: 60Β°C | ? mM Casein | free amino acids , Peptides | β | Incubation: 150 rpm | Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (90 days at 30Β°C), measured by absorbance spectrophotometry (colorimetric assay, FolinβCiocalteu reaction product with free tyrsines absorbance measurement, 660 nm) in aqueous phase | Benzene | 60% | 100 | 60 | % | Incubation: ?, Assay: 50 mM Na2HPO4βNaOH buffer, 5mM CoCl2 | Incubation: ?, Assay: 10 | Incubation: 30Β°C, Assay: 60Β°C | ? mM Casein | free amino acids , Peptides | β | Incubation: 150 rpm | Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (90 days at 30Β°C), measured by absorbance spectrophotometry (colorimetric assay, FolinβCiocalteu reaction product with free tyrsines absorbance measurement, 660 nm) in aqueous phase | Chloroform | 60% | 100 | 83 | % | Incubation: ?, Assay: 50 mM Na2HPO4βNaOH buffer, 5mM CoCl2 | Incubation: ?, Assay: 10 | Incubation: 30Β°C, Assay: 60Β°C | ? mM Casein | free amino acids , Peptides | β | Incubation: 150 rpm | Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (90 days at 30Β°C), measured by absorbance spectrophotometry (colorimetric assay, FolinβCiocalteu reaction product with free tyrsines absorbance measurement, 660 nm) in aqueous phase | n-Hexadecane | 60% | 100 | 95 | % | Incubation: ?, Assay: 50 mM Na2HPO4βNaOH buffer, 5mM CoCl2 | Incubation: ?, Assay: 10 | Incubation: 30Β°C, Assay: 60Β°C | ? mM Casein | free amino acids , Peptides | β | Incubation: 150 rpm | Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (90 days at 30Β°C), measured by absorbance spectrophotometry (colorimetric assay, FolinβCiocalteu reaction product with free tyrsines absorbance measurement, 660 nm) in aqueous phase | 1-Butanol | 60% | 100 | 38 | % | Incubation: ?, Assay: 50 mM Na2HPO4βNaOH buffer, 5mM CoCl2 | Incubation: ?, Assay: 10 | Incubation: 30Β°C, Assay: 60Β°C | ? mM Casein | free amino acids , Peptides | β | Incubation: 150 rpm | Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (90 days at 30Β°C), measured by absorbance spectrophotometry (colorimetric assay, FolinβCiocalteu reaction product with free tyrsines absorbance measurement, 660 nm) in aqueous phase | Ethyl Acetate | 60% | 100 | 0 | % | Incubation: ?, Assay: 50 mM Na2HPO4βNaOH buffer, 5mM CoCl2 | Incubation: ?, Assay: 10 | Incubation: 30Β°C, Assay: 60Β°C | ? mM Casein | free amino acids , Peptides | β | Incubation: 150 rpm | Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (90 days at 30Β°C), measured by absorbance spectrophotometry (colorimetric assay, FolinβCiocalteu reaction product with free tyrsines absorbance measurement, 660 nm) in aqueous phase | Isopropanol | 60% | 100 | 91 | % | Incubation: ?, Assay: 50 mM Na2HPO4βNaOH buffer, 5mM CoCl2 | Incubation: ?, Assay: 10 | Incubation: 30Β°C, Assay: 60Β°C | ? mM Casein | free amino acids , Peptides | β | Incubation: 150 rpm | Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (90 days at 30Β°C), measured by absorbance spectrophotometry (colorimetric assay, FolinβCiocalteu reaction product with free tyrsines absorbance measurement, 660 nm) in aqueous phase | Isopropanol | 60% | 100 | 88 | % | Incubation: ?, Assay: 50 mM Na2HPO4βNaOH buffer, 5mM CoCl2 | Incubation: ?, Assay: 10 | Incubation: 30Β°C, Assay: 60Β°C | ? mM Casein | free amino acids , Peptides | β | Incubation: 150 rpm | Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (90 days at 30Β°C), measured by absorbance spectrophotometry (colorimetric assay, FolinβCiocalteu reaction product with free tyrsines absorbance measurement, 660 nm) in aqueous phase | Acetonitrile | 60% | 100 | 0 | % | Incubation: ?, Assay: 50 mM Na2HPO4βNaOH buffer, 5mM CoCl2 | Incubation: ?, Assay: 10 | Incubation: 30Β°C, Assay: 60Β°C | ? mM Casein | free amino acids , Peptides | β | Incubation: 150 rpm | Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (90 days at 30Β°C), measured by absorbance spectrophotometry (colorimetric assay, FolinβCiocalteu reaction product with free tyrsines absorbance measurement, 660 nm) in aqueous phase | Ethanol | 60% | 100 | 71 | % | Incubation: ?, Assay: 50 mM Na2HPO4βNaOH buffer, 5mM CoCl2 | Incubation: ?, Assay: 10 | Incubation: 30Β°C, Assay: 60Β°C | ? mM Casein | free amino acids , Peptides | β | Incubation: 150 rpm | Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (90 days at 30Β°C), measured by absorbance spectrophotometry (colorimetric assay, FolinβCiocalteu reaction product with free tyrsines absorbance measurement, 660 nm) in aqueous phase | Methanol | 60% | 100 | 75 | % | Incubation: ?, Assay: 50 mM Na2HPO4βNaOH buffer, 5mM CoCl2 | Incubation: ?, Assay: 10 | Incubation: 30Β°C, Assay: 60Β°C | ? mM Casein | free amino acids , Peptides | β | Incubation: 150 rpm | Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (90 days at 30Β°C), measured by absorbance spectrophotometry (colorimetric assay, FolinβCiocalteu reaction product with free tyrsines absorbance measurement, 660 nm) in aqueous phase | Dimethylformamide (DMF) | 60% | 100 | 115 | % | Incubation: ?, Assay: 50 mM Na2HPO4βNaOH buffer, 5mM CoCl2 | Incubation: ?, Assay: 10 | Incubation: 30Β°C, Assay: 60Β°C | ? mM Casein | free amino acids , Peptides | β | Incubation: 150 rpm | Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (90 days at 30Β°C), measured by absorbance spectrophotometry (colorimetric assay, FolinβCiocalteu reaction product with free tyrsines absorbance measurement, 660 nm) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 60% | 100 | 127 | % | Incubation: ?, Assay: 50 mM Na2HPO4βNaOH buffer, 5mM CoCl2 | Incubation: ?, Assay: 10 | Incubation: 30Β°C, Assay: 60Β°C | ? mM Casein | free amino acids , Peptides | β | Incubation: 150 rpm | Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
Visualization : Stability β Incubation
Bassem Jaouadi et al. (2013)
One bar per measurement. Colour = solvent, shade = solvent volume.