Esterase BSE01701 from Bacillus sp. SCSIO 15121

Enzyme Description

Species

Extremophile

EC Number

3.1.1.1 ↗

Sequence

Length: 255 amino acids

MIQIENQAVSGIPFLHIVKEENRHRAVPLVIFIHGFTSAKEHNLHIAYLLAEKGFRAVLPEALHHGERGEEMAVEELAGHFWDIVLNEIEEIGVLKNHFEKEGLVDGGRIGLAGTSMGGITTLGALTAYDWIKAGVSLMGSPNYVELFQQQIDHIQSQGIDIDVPEEKVQQLMKRLELRDLSLQPEKLQQRPLLFWHGAKDKVVPYAPTRKFYDTIKSHYSEQPERLQFIGDENADHKVPRAAVLKTIEWFETYL

Jinlong Huang et al. (2016) — Functional Characterization of a Marine Bacillus Esterase and its Utilization in the Stereo-Selective Production of D-Methyl Lactate

Applied Biochemistry and Biotechnology · doi:10.1007/s12010-016-2180-y ↗ · Stability - Incubation Specificity - Enantioselectivity Activity + Stability - Conversion

54 measurements ▼

Database ID

UniParc: UPI00059DAC50 ↗

Sequence Annotation

Explicit - Provided GenBank Accession Number

Protein Source

Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (54 measurements)

54 measurements — page 1 of 3

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Activity + Stability - Conversion	Conversion (in %) after 30 minutes incubation at 35°C, determined by chiral GC in the presence of organic solvent	n-Heptane	80%	38.25	41.4	%	100 mM Tris-HCl buffer, 0.064 mg/mL enzyme concentraton	9	35°C	50 mM Methyl lactate	Lactic acid , Methanol	—	200 rpm	Classical aqueous control (in %)
Activity + Stability - Conversion	Conversion (in %) after 30 minutes incubation at 35°C, determined by chiral GC in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	5%	62.0	61.41	%	100 mM Tris-HCl buffer, 0.12 mg/mL enzyme concentration	9	35°C	50 mM Methyl lactate	Lactic acid , Methanol	—	200 rpm	Classical aqueous control (in %)
Activity + Stability - Conversion	Conversion (in %) after 30 minutes incubation at 35°C, determined by chiral GC in the presence of organic solvent	Acetone	5%	62.0	54.9	%	100 mM Tris-HCl buffer, 0.12 mg/mL enzyme concentration	9	35°C	50 mM Methyl lactate	Lactic acid , Methanol	—	200 rpm	Classical aqueous control (in %)
Activity + Stability - Conversion	Conversion (in %) after 30 minutes incubation at 35°C, determined by chiral GC in the presence of organic solvent	1-Propanol	5%	62.0	52.9	%	100 mM Tris-HCl buffer, 0.12 mg/mL enzyme concentration	9	35°C	50 mM Methyl lactate	Lactic acid , Methanol	—	200 rpm	Classical aqueous control (in %)
Activity + Stability - Conversion	Conversion (in %) after 30 minutes incubation at 35°C, determined by chiral GC in the presence of organic solvent	n-Decane	5%	62.0	62.21	%	100 mM Tris-HCl buffer, 0.12 mg/mL enzyme concentration	9	35°C	50 mM Methyl lactate	Lactic acid , Methanol	—	200 rpm	Classical aqueous control (in %)
Activity + Stability - Conversion	Conversion (in %) after 30 minutes incubation at 35°C, determined by chiral GC in the presence of organic solvent	n-Heptane	5%	62.0	66.22	%	100 mM Tris-HCl buffer, 0.12 mg/mL enzyme concentration	9	35°C	50 mM Methyl lactate	Lactic acid , Methanol	—	200 rpm	Classical aqueous control (in %)
Activity + Stability - Conversion	Conversion (in %) after 30 minutes incubation at 35°C, determined by chiral GC in the presence of organic solvent	n-Hexane	5%	62.0	63.76	%	100 mM Tris-HCl buffer, 0.12 mg/mL enzyme concentration	9	35°C	50 mM Methyl lactate	Lactic acid , Methanol	—	200 rpm	Classical aqueous control (in %)
Activity + Stability - Conversion	Conversion (in %) after 30 minutes incubation at 35°C, determined by chiral GC in the presence of organic solvent	n-Heptane	10%	38.25	51.66	%	100 mM Tris-HCl buffer, 0.064 mg/mL enzyme concentraton	9	35°C	50 mM Methyl lactate	Lactic acid , Methanol	—	200 rpm	Classical aqueous control (in %)
Activity + Stability - Conversion	Conversion (in %) after 30 minutes incubation at 35°C, determined by chiral GC in the presence of organic solvent	n-Heptane	20%	38.25	54.35	%	100 mM Tris-HCl buffer, 0.064 mg/mL enzyme concentraton	9	35°C	50 mM Methyl lactate	Lactic acid , Methanol	—	200 rpm	Classical aqueous control (in %)
Activity + Stability - Conversion	Conversion (in %) after 30 minutes incubation at 35°C, determined by chiral GC in the presence of organic solvent	n-Heptane	30%	38.25	57.12	%	100 mM Tris-HCl buffer, 0.064 mg/mL enzyme concentraton	9	35°C	50 mM Methyl lactate	Lactic acid , Methanol	—	200 rpm	Classical aqueous control (in %)
Activity + Stability - Conversion	Conversion (in %) after 30 minutes incubation at 35°C, determined by chiral GC in the presence of organic solvent	n-Heptane	40%	38.25	58.91	%	100 mM Tris-HCl buffer, 0.064 mg/mL enzyme concentraton	9	35°C	50 mM Methyl lactate	Lactic acid , Methanol	—	200 rpm	Classical aqueous control (in %)
Activity + Stability - Conversion	Conversion (in %) after 30 minutes incubation at 35°C, determined by chiral GC in the presence of organic solvent	n-Heptane	50%	38.25	60.19	%	100 mM Tris-HCl buffer, 0.064 mg/mL enzyme concentraton	9	35°C	50 mM Methyl lactate	Lactic acid , Methanol	—	200 rpm	Classical aqueous control (in %)
Activity + Stability - Conversion	Conversion (in %) after 30 minutes incubation at 35°C, determined by chiral GC in the presence of organic solvent	n-Heptane	60%	38.25	60.99	%	100 mM Tris-HCl buffer, 0.064 mg/mL enzyme concentraton	9	35°C	50 mM Methyl lactate	Lactic acid , Methanol	—	200 rpm	Classical aqueous control (in %)
Activity + Stability - Conversion	Conversion (in %) after 30 minutes incubation at 35°C, determined by chiral GC in the presence of organic solvent	n-Heptane	70%	38.25	56.74	%	100 mM Tris-HCl buffer, 0.064 mg/mL enzyme concentraton	9	35°C	50 mM Methyl lactate	Lactic acid , Methanol	—	200 rpm	Classical aqueous control (in %)
Specificity - Enantioselectivity	Enantiomeric excess (toward s) after 30 minutes incubation at 35°C, determined by chiral GC in the presence of organic solvent	n-Heptane	5%	77.73	91.88	% enantiomeric excess	100 mM Tris-HCl buffer, 0.12 mg/mL enzyme concentration	9	35°C	50 mM Methyl lactate	Lactic acid , Methanol	—	200 rpm	Classical aqueous control (in % enantiomeric excess)
Specificity - Enantioselectivity	Enantiomeric excess (toward s) after 30 minutes incubation at 35°C, determined by chiral GC in the presence of organic solvent	1-Propanol	5%	77.73	58.37	% enantiomeric excess	100 mM Tris-HCl buffer, 0.12 mg/mL enzyme concentration	9	35°C	50 mM Methyl lactate	Lactic acid , Methanol	—	200 rpm	Classical aqueous control (in % enantiomeric excess)
Specificity - Enantioselectivity	Enantiomeric excess (toward s) after 30 minutes incubation at 35°C, determined by chiral GC in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	5%	77.73	78.69	% enantiomeric excess	100 mM Tris-HCl buffer, 0.12 mg/mL enzyme concentration	9	35°C	50 mM Methyl lactate	Lactic acid , Methanol	—	200 rpm	Classical aqueous control (in % enantiomeric excess)
Specificity - Enantioselectivity	Enantiomeric excess (toward s) after 30 minutes incubation at 35°C, determined by chiral GC in the presence of organic solvent	Acetone	5%	77.73	60.49	% enantiomeric excess	100 mM Tris-HCl buffer, 0.12 mg/mL enzyme concentration	9	35°C	50 mM Methyl lactate	Lactic acid , Methanol	—	200 rpm	Classical aqueous control (in % enantiomeric excess)
Specificity - Enantioselectivity	Enantiomeric excess (toward s) after 30 minutes incubation at 35°C, determined by chiral GC in the presence of organic solvent	n-Decane	5%	77.73	87.39	% enantiomeric excess	100 mM Tris-HCl buffer, 0.12 mg/mL enzyme concentration	9	35°C	50 mM Methyl lactate	Lactic acid , Methanol	—	200 rpm	Classical aqueous control (in % enantiomeric excess)
Specificity - Enantioselectivity	Enantiomeric excess (toward s) after 30 minutes incubation at 35°C, determined by chiral GC in the presence of organic solvent	n-Heptane	10%	49.77	71.05	% enantiomeric excess	100 mM Tris-HCl buffer, 0.064 mg/mL enzyme concentraton	9	35°C	50 mM Methyl lactate	Lactic acid , Methanol	—	200 rpm	Classical aqueous control (in % enantiomeric excess)

Activity + Stability - Conversion

Conversion (in %) after 30 minutes incubation at 35°C, determined by chiral GC in the presence of organic solvent

n-Heptane

80%

38.25

41.4

100 mM Tris-HCl buffer, 0.064 mg/mL enzyme concentraton

35°C

50 mM Methyl lactate

Lactic acid, Methanol

—

200 rpm

Classical aqueous control (in %)

Activity + Stability - Conversion

Conversion (in %) after 30 minutes incubation at 35°C, determined by chiral GC in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

62.0

61.41

100 mM Tris-HCl buffer, 0.12 mg/mL enzyme concentration

35°C

50 mM Methyl lactate

Lactic acid, Methanol

—

200 rpm

Classical aqueous control (in %)

Activity + Stability - Conversion

Conversion (in %) after 30 minutes incubation at 35°C, determined by chiral GC in the presence of organic solvent

Acetone

62.0

54.9

100 mM Tris-HCl buffer, 0.12 mg/mL enzyme concentration

35°C

50 mM Methyl lactate

Lactic acid, Methanol

—

200 rpm

Classical aqueous control (in %)

Activity + Stability - Conversion

Conversion (in %) after 30 minutes incubation at 35°C, determined by chiral GC in the presence of organic solvent

1-Propanol

62.0

52.9

100 mM Tris-HCl buffer, 0.12 mg/mL enzyme concentration

35°C

50 mM Methyl lactate

Lactic acid, Methanol

—

200 rpm

Classical aqueous control (in %)

Activity + Stability - Conversion

Conversion (in %) after 30 minutes incubation at 35°C, determined by chiral GC in the presence of organic solvent

n-Decane

62.0

62.21

100 mM Tris-HCl buffer, 0.12 mg/mL enzyme concentration

35°C

50 mM Methyl lactate

Lactic acid, Methanol

—

200 rpm

Classical aqueous control (in %)

Activity + Stability - Conversion

Conversion (in %) after 30 minutes incubation at 35°C, determined by chiral GC in the presence of organic solvent

n-Heptane

62.0

66.22

100 mM Tris-HCl buffer, 0.12 mg/mL enzyme concentration

35°C

50 mM Methyl lactate

Lactic acid, Methanol

—

200 rpm

Classical aqueous control (in %)

Activity + Stability - Conversion

Conversion (in %) after 30 minutes incubation at 35°C, determined by chiral GC in the presence of organic solvent

n-Hexane

62.0

63.76

100 mM Tris-HCl buffer, 0.12 mg/mL enzyme concentration

35°C

50 mM Methyl lactate

Lactic acid, Methanol

—

200 rpm

Classical aqueous control (in %)

Activity + Stability - Conversion

Conversion (in %) after 30 minutes incubation at 35°C, determined by chiral GC in the presence of organic solvent

n-Heptane

10%

38.25

51.66

100 mM Tris-HCl buffer, 0.064 mg/mL enzyme concentraton

35°C

50 mM Methyl lactate

Lactic acid, Methanol

—

200 rpm

Classical aqueous control (in %)

Activity + Stability - Conversion

Conversion (in %) after 30 minutes incubation at 35°C, determined by chiral GC in the presence of organic solvent

n-Heptane

20%

38.25

54.35

100 mM Tris-HCl buffer, 0.064 mg/mL enzyme concentraton

35°C

50 mM Methyl lactate

Lactic acid, Methanol

—

200 rpm

Classical aqueous control (in %)

Activity + Stability - Conversion

Conversion (in %) after 30 minutes incubation at 35°C, determined by chiral GC in the presence of organic solvent

n-Heptane

30%

38.25

57.12

100 mM Tris-HCl buffer, 0.064 mg/mL enzyme concentraton

35°C

50 mM Methyl lactate

Lactic acid, Methanol

—

200 rpm

Classical aqueous control (in %)

Activity + Stability - Conversion

Conversion (in %) after 30 minutes incubation at 35°C, determined by chiral GC in the presence of organic solvent

n-Heptane

40%

38.25

58.91

100 mM Tris-HCl buffer, 0.064 mg/mL enzyme concentraton

35°C

50 mM Methyl lactate

Lactic acid, Methanol

—

200 rpm

Classical aqueous control (in %)

Activity + Stability - Conversion

Conversion (in %) after 30 minutes incubation at 35°C, determined by chiral GC in the presence of organic solvent

n-Heptane

50%

38.25

60.19

100 mM Tris-HCl buffer, 0.064 mg/mL enzyme concentraton

35°C

50 mM Methyl lactate

Lactic acid, Methanol

—

200 rpm

Classical aqueous control (in %)

Activity + Stability - Conversion

Conversion (in %) after 30 minutes incubation at 35°C, determined by chiral GC in the presence of organic solvent

n-Heptane

60%

38.25

60.99

100 mM Tris-HCl buffer, 0.064 mg/mL enzyme concentraton

35°C

50 mM Methyl lactate

Lactic acid, Methanol

—

200 rpm

Classical aqueous control (in %)

Activity + Stability - Conversion

Conversion (in %) after 30 minutes incubation at 35°C, determined by chiral GC in the presence of organic solvent

n-Heptane

70%

38.25

56.74

100 mM Tris-HCl buffer, 0.064 mg/mL enzyme concentraton

35°C

50 mM Methyl lactate

Lactic acid, Methanol

—

200 rpm

Classical aqueous control (in %)

Specificity - Enantioselectivity

Enantiomeric excess (toward s) after 30 minutes incubation at 35°C, determined by chiral GC in the presence of organic solvent

n-Heptane

77.73

91.88

% enantiomeric excess

100 mM Tris-HCl buffer, 0.12 mg/mL enzyme concentration

35°C

50 mM Methyl lactate

Lactic acid, Methanol

—

200 rpm

Classical aqueous control (in % enantiomeric excess)

Specificity - Enantioselectivity

Enantiomeric excess (toward s) after 30 minutes incubation at 35°C, determined by chiral GC in the presence of organic solvent

1-Propanol

77.73

58.37

% enantiomeric excess

100 mM Tris-HCl buffer, 0.12 mg/mL enzyme concentration

35°C

50 mM Methyl lactate

Lactic acid, Methanol

—

200 rpm

Classical aqueous control (in % enantiomeric excess)

Specificity - Enantioselectivity

Enantiomeric excess (toward s) after 30 minutes incubation at 35°C, determined by chiral GC in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

77.73

78.69

% enantiomeric excess

100 mM Tris-HCl buffer, 0.12 mg/mL enzyme concentration

35°C

50 mM Methyl lactate

Lactic acid, Methanol

—

200 rpm

Classical aqueous control (in % enantiomeric excess)

Specificity - Enantioselectivity

Enantiomeric excess (toward s) after 30 minutes incubation at 35°C, determined by chiral GC in the presence of organic solvent

Acetone

77.73

60.49

% enantiomeric excess

100 mM Tris-HCl buffer, 0.12 mg/mL enzyme concentration

35°C

50 mM Methyl lactate

Lactic acid, Methanol

—

200 rpm

Classical aqueous control (in % enantiomeric excess)

Specificity - Enantioselectivity

Enantiomeric excess (toward s) after 30 minutes incubation at 35°C, determined by chiral GC in the presence of organic solvent

n-Decane

77.73

87.39

% enantiomeric excess

100 mM Tris-HCl buffer, 0.12 mg/mL enzyme concentration

35°C

50 mM Methyl lactate

Lactic acid, Methanol

—

200 rpm

Classical aqueous control (in % enantiomeric excess)

Specificity - Enantioselectivity

Enantiomeric excess (toward s) after 30 minutes incubation at 35°C, determined by chiral GC in the presence of organic solvent

n-Heptane

10%

49.77

71.05

% enantiomeric excess

100 mM Tris-HCl buffer, 0.064 mg/mL enzyme concentraton

35°C

50 mM Methyl lactate

Lactic acid, Methanol

—

200 rpm

Classical aqueous control (in % enantiomeric excess)

Specificity - Enantioselectivity

Enantiomeric excess (toward s) after 30 minutes incubation at 35°C, determined by chiral GC in the presence of organic solvent

n-Heptane

20%

49.77

78.19

% enantiomeric excess

100 mM Tris-HCl buffer, 0.064 mg/mL enzyme concentraton

35°C

50 mM Methyl lactate

Lactic acid, Methanol

—

200 rpm

Classical aqueous control (in % enantiomeric excess)

Specificity - Enantioselectivity

Enantiomeric excess (toward s) after 30 minutes incubation at 35°C, determined by chiral GC in the presence of organic solvent

n-Heptane

30%

49.77

89.15

% enantiomeric excess

100 mM Tris-HCl buffer, 0.064 mg/mL enzyme concentraton

35°C

50 mM Methyl lactate

Lactic acid, Methanol

—

200 rpm

Classical aqueous control (in % enantiomeric excess)

Specificity - Enantioselectivity

Enantiomeric excess (toward s) after 30 minutes incubation at 35°C, determined by chiral GC in the presence of organic solvent

n-Heptane

40%

49.77

93.05

% enantiomeric excess

100 mM Tris-HCl buffer, 0.064 mg/mL enzyme concentraton

35°C

50 mM Methyl lactate

Lactic acid, Methanol

—

200 rpm

Classical aqueous control (in % enantiomeric excess)

Specificity - Enantioselectivity

Enantiomeric excess (toward s) after 30 minutes incubation at 35°C, determined by chiral GC in the presence of organic solvent

n-Heptane

50%

49.77

96.69

% enantiomeric excess

100 mM Tris-HCl buffer, 0.064 mg/mL enzyme concentraton

35°C

50 mM Methyl lactate

Lactic acid, Methanol

—

200 rpm

Classical aqueous control (in % enantiomeric excess)

Specificity - Enantioselectivity

Enantiomeric excess (toward s) after 30 minutes incubation at 35°C, determined by chiral GC in the presence of organic solvent

n-Heptane

60%

49.77

98.19

% enantiomeric excess

100 mM Tris-HCl buffer, 0.064 mg/mL enzyme concentraton

35°C

50 mM Methyl lactate

Lactic acid, Methanol

—

200 rpm

Classical aqueous control (in % enantiomeric excess)

Specificity - Enantioselectivity

Enantiomeric excess (toward s) after 30 minutes incubation at 35°C, determined by chiral GC in the presence of organic solvent

n-Heptane

70%

49.77

90.49

% enantiomeric excess

100 mM Tris-HCl buffer, 0.064 mg/mL enzyme concentraton

35°C

50 mM Methyl lactate

Lactic acid, Methanol

—

200 rpm

Classical aqueous control (in % enantiomeric excess)

Specificity - Enantioselectivity

Enantiomeric excess (toward s) after 30 minutes incubation at 35°C, determined by chiral GC in the presence of organic solvent

n-Heptane

80%

49.77

50.83

% enantiomeric excess

100 mM Tris-HCl buffer, 0.064 mg/mL enzyme concentraton

35°C

50 mM Methyl lactate

Lactic acid, Methanol

—

200 rpm

Classical aqueous control (in % enantiomeric excess)

Specificity - Enantioselectivity

Enantiomeric excess (toward s) after 30 minutes incubation at 35°C, determined by chiral GC in the presence of organic solvent

n-Hexane

77.73

86.25

% enantiomeric excess

100 mM Tris-HCl buffer, 0.12 mg/mL enzyme concentration

35°C

50 mM Methyl lactate

Lactic acid, Methanol

—

200 rpm

Classical aqueous control (in % enantiomeric excess)

Stability - Incubation

Activity after incubation (1 hour at 35°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase

n-Heptane

100.0

98.56

Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer

Incubation: 8.5, Assay: 8.5

Incubation: 35°C, Assay: 35°C

0.2 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Non-incubated control (in %), assay measured in aqueous phase

Stability - Incubation

Activity after incubation (1 hour at 35°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase

n-Hexane

100.0

111.2

Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer

Incubation: 8.5, Assay: 8.5

Incubation: 35°C, Assay: 35°C

0.2 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Non-incubated control (in %), assay measured in aqueous phase

Stability - Incubation

Activity after incubation (1 hour at 35°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase

n-Decane

100.0

89.54

Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer

Incubation: 8.5, Assay: 8.5

Incubation: 35°C, Assay: 35°C

0.2 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Non-incubated control (in %), assay measured in aqueous phase

Stability - Incubation

Activity after incubation (1 hour at 35°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase

Methanol

100.0

106.49

Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer

Incubation: 8.5, Assay: 8.5

Incubation: 35°C, Assay: 35°C

0.2 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Non-incubated control (in %), assay measured in aqueous phase

Stability - Incubation

Activity after incubation (1 hour at 35°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase

1-Propanol

100.0

99.44

Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer

Incubation: 8.5, Assay: 8.5

Incubation: 35°C, Assay: 35°C

0.2 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Non-incubated control (in %), assay measured in aqueous phase

Stability - Incubation

Activity after incubation (1 hour at 35°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase

1-Octanol

100.0

78.54

Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer

Incubation: 8.5, Assay: 8.5

Incubation: 35°C, Assay: 35°C

0.2 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Non-incubated control (in %), assay measured in aqueous phase

Stability - Incubation

Activity after incubation (1 hour at 35°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase

Dimethyl Sulfoxide (DMSO)

100.0

101.24

Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer

Incubation: 8.5, Assay: 8.5

Incubation: 35°C, Assay: 35°C

0.2 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Non-incubated control (in %), assay measured in aqueous phase

Stability - Incubation

Activity after incubation (1 hour at 35°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase

Tetrahydrofuran (THF)

100.0

66.48

Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer

Incubation: 8.5, Assay: 8.5

Incubation: 35°C, Assay: 35°C

0.2 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Non-incubated control (in %), assay measured in aqueous phase

Stability - Incubation

Activity after incubation (1 hour at 35°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase

1,4-Dioxane

100.0

39.55

Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer

Incubation: 8.5, Assay: 8.5

Incubation: 35°C, Assay: 35°C

0.2 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Non-incubated control (in %), assay measured in aqueous phase

Stability - Incubation

Activity after incubation (1 hour at 35°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase

Toluene

100.0

53.55

Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer

Incubation: 8.5, Assay: 8.5

Incubation: 35°C, Assay: 35°C

0.2 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Non-incubated control (in %), assay measured in aqueous phase

Stability - Incubation

Activity after incubation (1 hour at 35°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase

Xylene

100.0

55.65

Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer

Incubation: 8.5, Assay: 8.5

Incubation: 35°C, Assay: 35°C

0.2 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Non-incubated control (in %), assay measured in aqueous phase

Stability - Incubation

Activity after incubation (1 hour at 35°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase

Acetone

100.0

103.89

Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer

Incubation: 8.5, Assay: 8.5

Incubation: 35°C, Assay: 35°C

0.2 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Non-incubated control (in %), assay measured in aqueous phase

Stability - Incubation

Activity after incubation (1 hour at 35°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase

Dichloromethane

100.0

26.39

Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer

Incubation: 8.5, Assay: 8.5

Incubation: 35°C, Assay: 35°C

0.2 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Non-incubated control (in %), assay measured in aqueous phase

Stability - Incubation

Activity after incubation (1 hour at 35°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase

n-Hexane

10%

100.0

97.51

Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer

Incubation: 8.5, Assay: 8.5

Incubation: 35°C, Assay: 35°C

0.2 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Non-incubated control (in %), assay measured in aqueous phase

Stability - Incubation

Activity after incubation (1 hour at 35°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase

n-Heptane

10%

100.0

110.39

Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer

Incubation: 8.5, Assay: 8.5

Incubation: 35°C, Assay: 35°C

0.2 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Non-incubated control (in %), assay measured in aqueous phase

Stability - Incubation

Activity after incubation (1 hour at 35°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase

n-Decane

10%

100.0

67.43

Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer

Incubation: 8.5, Assay: 8.5

Incubation: 35°C, Assay: 35°C

0.2 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Non-incubated control (in %), assay measured in aqueous phase

Stability - Incubation

Activity after incubation (1 hour at 35°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase

Methanol

10%

100.0

72.32

Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer

Incubation: 8.5, Assay: 8.5

Incubation: 35°C, Assay: 35°C

0.2 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Non-incubated control (in %), assay measured in aqueous phase

Stability - Incubation

Activity after incubation (1 hour at 35°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase

1-Propanol

10%

100.0

6.48

Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer

Incubation: 8.5, Assay: 8.5

Incubation: 35°C, Assay: 35°C

0.2 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Non-incubated control (in %), assay measured in aqueous phase

Stability - Incubation

Activity after incubation (1 hour at 35°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase

1-Octanol

10%

100.0

10.31

Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer

Incubation: 8.5, Assay: 8.5

Incubation: 35°C, Assay: 35°C

0.2 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Non-incubated control (in %), assay measured in aqueous phase

Stability - Incubation

Activity after incubation (1 hour at 35°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase

Dimethyl Sulfoxide (DMSO)

10%

100.0

100.41

Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer

Incubation: 8.5, Assay: 8.5

Incubation: 35°C, Assay: 35°C

0.2 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Non-incubated control (in %), assay measured in aqueous phase

Stability - Incubation

Activity after incubation (1 hour at 35°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase

Tetrahydrofuran (THF)

10%

100.0

4.15

Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer

Incubation: 8.5, Assay: 8.5

Incubation: 35°C, Assay: 35°C

0.2 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Non-incubated control (in %), assay measured in aqueous phase

Stability - Incubation

Activity after incubation (1 hour at 35°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase

1,4-Dioxane

10%

100.0

4.28

Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer

Incubation: 8.5, Assay: 8.5

Incubation: 35°C, Assay: 35°C

0.2 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Non-incubated control (in %), assay measured in aqueous phase

Stability - Incubation

Activity after incubation (1 hour at 35°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase

Toluene

10%

100.0

37.79

Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer

Incubation: 8.5, Assay: 8.5

Incubation: 35°C, Assay: 35°C

0.2 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Non-incubated control (in %), assay measured in aqueous phase

Stability - Incubation

Activity after incubation (1 hour at 35°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase

Xylene

10%

100.0

56.12

Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer

Incubation: 8.5, Assay: 8.5

Incubation: 35°C, Assay: 35°C

0.2 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Non-incubated control (in %), assay measured in aqueous phase

Stability - Incubation

Activity after incubation (1 hour at 35°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase

Acetone

10%

100.0

54.82

Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer

Incubation: 8.5, Assay: 8.5

Incubation: 35°C, Assay: 35°C

0.2 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Non-incubated control (in %), assay measured in aqueous phase

Stability - Incubation

Activity after incubation (1 hour at 35°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase

Dichloromethane

10%

100.0

40.0

Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer

Incubation: 8.5, Assay: 8.5

Incubation: 35°C, Assay: 35°C

0.2 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Non-incubated control (in %), assay measured in aqueous phase

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Visualization : Stability — Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Visualization : Specificity — Enantioselectivity

One bar per measurement. Colour = solvent, shade = solvent volume.

Visualization : Activity + Stability - Conversion

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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Esterase BSE01701 from Bacillus sp. SCSIO 15121

Enzyme Description

Sequence

Experimental Data (54 measurements)

Visualization : Stability — Incubation

Visualization : Specificity — Enantioselectivity

Visualization : Activity + Stability - Conversion

Structure

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