2-deoxy-D-ribose-5- phosphate aldolase from Haloarcula japonica TR-1
Enzyme Description
Species
Extremophile
Yes
"extreme halophilic" archaeon
EC Number
Sequence
MDDIPDRIEHTVLGPTTTPDDVRTCLDEALQYGMRACIPPWALPLATEYANVQLTAVIDFPHGQGQTDSVCQAAKLAWDAGADELDMVCNIGLLKAGEDDAVRNHITEVVASVPVPVKVIVEAPLLSDDELERVGQLVADADAAYLKTATGFSEGGATVHDVEILSKYLPVKASGGVGSWADAQAMFEAGAERIGASSGDTIVREWQAATEGETVTEPERDRDDPDTTDGY
Tatsuya Ohshida et al. (2016)
β First characterization of extremely halophilic 2-deoxy-D-ribose-5-phosphate aldolase
Protein Expression and Purification
Β· doi:10.1016/j.pep.2016.05.009 β
Β· Stability - Incubation
▼
Database ID
UniProt: A0A1Q2T258 β
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)
Experimental Data (44 measurements)
44 measurements β page 2 of 3
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Stability - Incubation | Activity after incubation (50 minutes at 25Β°C), measured by absorbance spectrophotometry NADH absorbance measurement in a coupled reaction with TIM and GPDH, with TIM converting G3P into DHAP, and GPDH reducing DHAP, 340 nm) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 50% | 100 | 101 | % | Incubation: 10 mM Tris-HCl buffer, 2M NaCl, Assay: 100 mM citrate-Na citrate buffer, 1 M NaCl, 0.15 mM NADH, 8 U/ml triose-phosphate isomerase (rabbit muscle), 2.5 U/ml glycerol-3-phosphate dehydrogenase (rabbit muscle) | Incubation: 8, Assay: 6.4 | Incubation: 25Β°C, Assay: 37Β°C | 2 mM 2-Deoxyribose-5-phosphate | Acetaldehyde , Glyceraldehyde 3-phosphate | β | β | Non-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase |
| Stability - Incubation | Activity after incubation (60 minutes at 25Β°C), measured by absorbance spectrophotometry NADH absorbance measurement in a coupled reaction with TIM and GPDH, with TIM converting G3P into DHAP, and GPDH reducing DHAP, 340 nm) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 50% | 100 | 97 | % | Incubation: 10 mM Tris-HCl buffer, 2M NaCl, Assay: 100 mM citrate-Na citrate buffer, 1 M NaCl, 0.15 mM NADH, 8 U/ml triose-phosphate isomerase (rabbit muscle), 2.5 U/ml glycerol-3-phosphate dehydrogenase (rabbit muscle) | Incubation: 8, Assay: 6.4 | Incubation: 25Β°C, Assay: 37Β°C | 2 mM 2-Deoxyribose-5-phosphate | Acetaldehyde , Glyceraldehyde 3-phosphate | β | β | Non-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase |
| Stability - Incubation | Activity after incubation (10 minutes at 25Β°C), measured by absorbance spectrophotometry NADH absorbance measurement in a coupled reaction with TIM and GPDH, with TIM converting G3P into DHAP, and GPDH reducing DHAP, 340 nm) in aqueous phase | Acetonitrile | 20% | 100 | 81 | % | Incubation: 10 mM Tris-HCl buffer, 2M NaCl, Assay: 100 mM citrate-Na citrate buffer, 1 M NaCl, 0.15 mM NADH, 8 U/ml triose-phosphate isomerase (rabbit muscle), 2.5 U/ml glycerol-3-phosphate dehydrogenase (rabbit muscle) | Incubation: 8, Assay: 6.4 | Incubation: 25Β°C, Assay: 37Β°C | 2 mM 2-Deoxyribose-5-phosphate | Acetaldehyde , Glyceraldehyde 3-phosphate | β | β | Non-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase |
| Stability - Incubation | Activity after incubation (10 minutes at 25Β°C), measured by absorbance spectrophotometry NADH absorbance measurement in a coupled reaction with TIM and GPDH, with TIM converting G3P into DHAP, and GPDH reducing DHAP, 340 nm) in aqueous phase | Ethanol | 20% | 100 | 87 | % | Incubation: 10 mM Tris-HCl buffer, 2M NaCl, Assay: 100 mM citrate-Na citrate buffer, 1 M NaCl, 0.15 mM NADH, 8 U/ml triose-phosphate isomerase (rabbit muscle), 2.5 U/ml glycerol-3-phosphate dehydrogenase (rabbit muscle) | Incubation: 8, Assay: 6.4 | Incubation: 25Β°C, Assay: 37Β°C | 2 mM 2-Deoxyribose-5-phosphate | Acetaldehyde , Glyceraldehyde 3-phosphate | β | β | Non-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase |
| Stability - Incubation | Activity after incubation (10 minutes at 25Β°C), measured by absorbance spectrophotometry NADH absorbance measurement in a coupled reaction with TIM and GPDH, with TIM converting G3P into DHAP, and GPDH reducing DHAP, 340 nm) in aqueous phase | Ethanol | 30% | 100 | 60 | % | Incubation: 10 mM Tris-HCl buffer, 2M NaCl, Assay: 100 mM citrate-Na citrate buffer, 1 M NaCl, 0.15 mM NADH, 8 U/ml triose-phosphate isomerase (rabbit muscle), 2.5 U/ml glycerol-3-phosphate dehydrogenase (rabbit muscle) | Incubation: 8, Assay: 6.4 | Incubation: 25Β°C, Assay: 37Β°C | 2 mM 2-Deoxyribose-5-phosphate | Acetaldehyde , Glyceraldehyde 3-phosphate | β | β | Non-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase |
| Stability - Incubation | Activity after incubation (10 minutes at 25Β°C), measured by absorbance spectrophotometry NADH absorbance measurement in a coupled reaction with TIM and GPDH, with TIM converting G3P into DHAP, and GPDH reducing DHAP, 340 nm) in aqueous phase | Ethanol | 40% | 100 | 46 | % | Incubation: 10 mM Tris-HCl buffer, 2M NaCl, Assay: 100 mM citrate-Na citrate buffer, 1 M NaCl, 0.15 mM NADH, 8 U/ml triose-phosphate isomerase (rabbit muscle), 2.5 U/ml glycerol-3-phosphate dehydrogenase (rabbit muscle) | Incubation: 8, Assay: 6.4 | Incubation: 25Β°C, Assay: 37Β°C | 2 mM 2-Deoxyribose-5-phosphate | Acetaldehyde , Glyceraldehyde 3-phosphate | β | β | Non-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase |
| Stability - Incubation | Activity after incubation (10 minutes at 25Β°C), measured by absorbance spectrophotometry NADH absorbance measurement in a coupled reaction with TIM and GPDH, with TIM converting G3P into DHAP, and GPDH reducing DHAP, 340 nm) in aqueous phase | Ethanol | 50% | 100 | 30 | % | Incubation: 10 mM Tris-HCl buffer, 2M NaCl, Assay: 100 mM citrate-Na citrate buffer, 1 M NaCl, 0.15 mM NADH, 8 U/ml triose-phosphate isomerase (rabbit muscle), 2.5 U/ml glycerol-3-phosphate dehydrogenase (rabbit muscle) | Incubation: 8, Assay: 6.4 | Incubation: 25Β°C, Assay: 37Β°C | 2 mM 2-Deoxyribose-5-phosphate | Acetaldehyde , Glyceraldehyde 3-phosphate | β | β | Non-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase |
| Stability - Incubation | Activity after incubation (10 minutes at 25Β°C), measured by absorbance spectrophotometry NADH absorbance measurement in a coupled reaction with TIM and GPDH, with TIM converting G3P into DHAP, and GPDH reducing DHAP, 340 nm) in aqueous phase | Methanol | 10% | 100 | 82 | % | Incubation: 10 mM Tris-HCl buffer, 2M NaCl, Assay: 100 mM citrate-Na citrate buffer, 1 M NaCl, 0.15 mM NADH, 8 U/ml triose-phosphate isomerase (rabbit muscle), 2.5 U/ml glycerol-3-phosphate dehydrogenase (rabbit muscle) | Incubation: 8, Assay: 6.4 | Incubation: 25Β°C, Assay: 37Β°C | 2 mM 2-Deoxyribose-5-phosphate | Acetaldehyde , Glyceraldehyde 3-phosphate | β | β | Non-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase |
| Stability - Incubation | Activity after incubation (10 minutes at 25Β°C), measured by absorbance spectrophotometry NADH absorbance measurement in a coupled reaction with TIM and GPDH, with TIM converting G3P into DHAP, and GPDH reducing DHAP, 340 nm) in aqueous phase | Methanol | 20% | 100 | 99 | % | Incubation: 10 mM Tris-HCl buffer, 2M NaCl, Assay: 100 mM citrate-Na citrate buffer, 1 M NaCl, 0.15 mM NADH, 8 U/ml triose-phosphate isomerase (rabbit muscle), 2.5 U/ml glycerol-3-phosphate dehydrogenase (rabbit muscle) | Incubation: 8, Assay: 6.4 | Incubation: 25Β°C, Assay: 37Β°C | 2 mM 2-Deoxyribose-5-phosphate | Acetaldehyde , Glyceraldehyde 3-phosphate | β | β | Non-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase |
| Stability - Incubation | Activity after incubation (10 minutes at 25Β°C), measured by absorbance spectrophotometry NADH absorbance measurement in a coupled reaction with TIM and GPDH, with TIM converting G3P into DHAP, and GPDH reducing DHAP, 340 nm) in aqueous phase | Methanol | 30% | 100 | 94 | % | Incubation: 10 mM Tris-HCl buffer, 2M NaCl, Assay: 100 mM citrate-Na citrate buffer, 1 M NaCl, 0.15 mM NADH, 8 U/ml triose-phosphate isomerase (rabbit muscle), 2.5 U/ml glycerol-3-phosphate dehydrogenase (rabbit muscle) | Incubation: 8, Assay: 6.4 | Incubation: 25Β°C, Assay: 37Β°C | 2 mM 2-Deoxyribose-5-phosphate | Acetaldehyde , Glyceraldehyde 3-phosphate | β | β | Non-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase |
| Stability - Incubation | Activity after incubation (10 minutes at 25Β°C), measured by absorbance spectrophotometry NADH absorbance measurement in a coupled reaction with TIM and GPDH, with TIM converting G3P into DHAP, and GPDH reducing DHAP, 340 nm) in aqueous phase | Methanol | 40% | 100 | 60 | % | Incubation: 10 mM Tris-HCl buffer, 2M NaCl, Assay: 100 mM citrate-Na citrate buffer, 1 M NaCl, 0.15 mM NADH, 8 U/ml triose-phosphate isomerase (rabbit muscle), 2.5 U/ml glycerol-3-phosphate dehydrogenase (rabbit muscle) | Incubation: 8, Assay: 6.4 | Incubation: 25Β°C, Assay: 37Β°C | 2 mM 2-Deoxyribose-5-phosphate | Acetaldehyde , Glyceraldehyde 3-phosphate | β | β | Non-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase |
| Stability - Incubation | Activity after incubation (10 minutes at 25Β°C), measured by absorbance spectrophotometry NADH absorbance measurement in a coupled reaction with TIM and GPDH, with TIM converting G3P into DHAP, and GPDH reducing DHAP, 340 nm) in aqueous phase | Methanol | 50% | 100 | 23 | % | Incubation: 10 mM Tris-HCl buffer, 2M NaCl, Assay: 100 mM citrate-Na citrate buffer, 1 M NaCl, 0.15 mM NADH, 8 U/ml triose-phosphate isomerase (rabbit muscle), 2.5 U/ml glycerol-3-phosphate dehydrogenase (rabbit muscle) | Incubation: 8, Assay: 6.4 | Incubation: 25Β°C, Assay: 37Β°C | 2 mM 2-Deoxyribose-5-phosphate | Acetaldehyde , Glyceraldehyde 3-phosphate | β | β | Non-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase |
| Stability - Incubation | Activity after incubation (10 minutes at 25Β°C), measured by absorbance spectrophotometry NADH absorbance measurement in a coupled reaction with TIM and GPDH, with TIM converting G3P into DHAP, and GPDH reducing DHAP, 340 nm) in aqueous phase | Acetonitrile | 10% | 100 | 96 | % | Incubation: 10 mM Tris-HCl buffer, 2M NaCl, Assay: 100 mM citrate-Na citrate buffer, 1 M NaCl, 0.15 mM NADH, 8 U/ml triose-phosphate isomerase (rabbit muscle), 2.5 U/ml glycerol-3-phosphate dehydrogenase (rabbit muscle) | Incubation: 8, Assay: 6.4 | Incubation: 25Β°C, Assay: 37Β°C | 2 mM 2-Deoxyribose-5-phosphate | Acetaldehyde , Glyceraldehyde 3-phosphate | β | β | Non-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase |
| Stability - Incubation | Activity after incubation (10 minutes at 25Β°C), measured by absorbance spectrophotometry NADH absorbance measurement in a coupled reaction with TIM and GPDH, with TIM converting G3P into DHAP, and GPDH reducing DHAP, 340 nm) in aqueous phase | Ethanol | 10% | 100 | 86 | % | Incubation: 10 mM Tris-HCl buffer, 2M NaCl, Assay: 100 mM citrate-Na citrate buffer, 1 M NaCl, 0.15 mM NADH, 8 U/ml triose-phosphate isomerase (rabbit muscle), 2.5 U/ml glycerol-3-phosphate dehydrogenase (rabbit muscle) | Incubation: 8, Assay: 6.4 | Incubation: 25Β°C, Assay: 37Β°C | 2 mM 2-Deoxyribose-5-phosphate | Acetaldehyde , Glyceraldehyde 3-phosphate | β | β | Non-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase |
| Stability - Incubation | Activity after incubation (10 minutes at 25Β°C), measured by absorbance spectrophotometry NADH absorbance measurement in a coupled reaction with TIM and GPDH, with TIM converting G3P into DHAP, and GPDH reducing DHAP, 340 nm) in aqueous phase | Acetonitrile | 30% | 100 | 85 | % | Incubation: 10 mM Tris-HCl buffer, 2M NaCl, Assay: 100 mM citrate-Na citrate buffer, 1 M NaCl, 0.15 mM NADH, 8 U/ml triose-phosphate isomerase (rabbit muscle), 2.5 U/ml glycerol-3-phosphate dehydrogenase (rabbit muscle) | Incubation: 8, Assay: 6.4 | Incubation: 25Β°C, Assay: 37Β°C | 2 mM 2-Deoxyribose-5-phosphate | Acetaldehyde , Glyceraldehyde 3-phosphate | β | β | Non-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase |
| Stability - Incubation | Activity after incubation (10 minutes at 25Β°C), measured by absorbance spectrophotometry NADH absorbance measurement in a coupled reaction with TIM and GPDH, with TIM converting G3P into DHAP, and GPDH reducing DHAP, 340 nm) in aqueous phase | Acetonitrile | 40% | 100 | 83 | % | Incubation: 10 mM Tris-HCl buffer, 2M NaCl, Assay: 100 mM citrate-Na citrate buffer, 1 M NaCl, 0.15 mM NADH, 8 U/ml triose-phosphate isomerase (rabbit muscle), 2.5 U/ml glycerol-3-phosphate dehydrogenase (rabbit muscle) | Incubation: 8, Assay: 6.4 | Incubation: 25Β°C, Assay: 37Β°C | 2 mM 2-Deoxyribose-5-phosphate | Acetaldehyde , Glyceraldehyde 3-phosphate | β | β | Non-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase |
| Stability - Incubation | Activity after incubation (10 minutes at 25Β°C), measured by absorbance spectrophotometry NADH absorbance measurement in a coupled reaction with TIM and GPDH, with TIM converting G3P into DHAP, and GPDH reducing DHAP, 340 nm) in aqueous phase | Acetonitrile | 50% | 100 | 84 | % | Incubation: 10 mM Tris-HCl buffer, 2M NaCl, Assay: 100 mM citrate-Na citrate buffer, 1 M NaCl, 0.15 mM NADH, 8 U/ml triose-phosphate isomerase (rabbit muscle), 2.5 U/ml glycerol-3-phosphate dehydrogenase (rabbit muscle) | Incubation: 8, Assay: 6.4 | Incubation: 25Β°C, Assay: 37Β°C | 2 mM 2-Deoxyribose-5-phosphate | Acetaldehyde , Glyceraldehyde 3-phosphate | β | β | Non-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase |
| Stability - Incubation | Activity after incubation (10 minutes at 25Β°C), measured by absorbance spectrophotometry NADH absorbance measurement in a coupled reaction with TIM and GPDH, with TIM converting G3P into DHAP, and GPDH reducing DHAP, 340 nm) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 10% | 100 | 94 | % | Incubation: 10 mM Tris-HCl buffer, 2M NaCl, Assay: 100 mM citrate-Na citrate buffer, 1 M NaCl, 0.15 mM NADH, 8 U/ml triose-phosphate isomerase (rabbit muscle), 2.5 U/ml glycerol-3-phosphate dehydrogenase (rabbit muscle) | Incubation: 8, Assay: 6.4 | Incubation: 25Β°C, Assay: 37Β°C | 2 mM 2-Deoxyribose-5-phosphate | Acetaldehyde , Glyceraldehyde 3-phosphate | β | β | Non-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase |
| Stability - Incubation | Activity after incubation (10 minutes at 25Β°C), measured by absorbance spectrophotometry NADH absorbance measurement in a coupled reaction with TIM and GPDH, with TIM converting G3P into DHAP, and GPDH reducing DHAP, 340 nm) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 20% | 100 | 96 | % | Incubation: 10 mM Tris-HCl buffer, 2M NaCl, Assay: 100 mM citrate-Na citrate buffer, 1 M NaCl, 0.15 mM NADH, 8 U/ml triose-phosphate isomerase (rabbit muscle), 2.5 U/ml glycerol-3-phosphate dehydrogenase (rabbit muscle) | Incubation: 8, Assay: 6.4 | Incubation: 25Β°C, Assay: 37Β°C | 2 mM 2-Deoxyribose-5-phosphate | Acetaldehyde , Glyceraldehyde 3-phosphate | β | β | Non-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase |
| Stability - Incubation | Activity after incubation (10 minutes at 25Β°C), measured by absorbance spectrophotometry NADH absorbance measurement in a coupled reaction with TIM and GPDH, with TIM converting G3P into DHAP, and GPDH reducing DHAP, 340 nm) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 30% | 100 | 98 | % | Incubation: 10 mM Tris-HCl buffer, 2M NaCl, Assay: 100 mM citrate-Na citrate buffer, 1 M NaCl, 0.15 mM NADH, 8 U/ml triose-phosphate isomerase (rabbit muscle), 2.5 U/ml glycerol-3-phosphate dehydrogenase (rabbit muscle) | Incubation: 8, Assay: 6.4 | Incubation: 25Β°C, Assay: 37Β°C | 2 mM 2-Deoxyribose-5-phosphate | Acetaldehyde , Glyceraldehyde 3-phosphate | β | β | Non-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase |
Visualization : Stability β Incubation
One bar per measurement. Colour = solvent, shade = solvent volume.