2-deoxy-D-ribose-5- phosphate aldolase from Haloarcula japonica TR-1

Enzyme Description

Species

Haloarcula japonica TR-1 ↗

Extremophile

Yes "extreme halophilic" archaeon

EC Number

4.1.2.4 ↗

Sequence

Length: 231 amino acids

MDDIPDRIEHTVLGPTTTPDDVRTCLDEALQYGMRACIPPWALPLATEYANVQLTAVIDFPHGQGQTDSVCQAAKLAWDAGADELDMVCNIGLLKAGEDDAVRNHITEVVASVPVPVKVIVEAPLLSDDELERVGQLVADADAAYLKTATGFSEGGATVHDVEILSKYLPVKASGGVGSWADAQAMFEAGAERIGASSGDTIVREWQAATEGETVTEPERDRDDPDTTDGY

Tatsuya Ohshida et al. (2016) — First characterization of extremely halophilic 2-deoxy-D-ribose-5-phosphate aldolase

Protein Expression and Purification · doi:10.1016/j.pep.2016.05.009 ↗ · Stability - Incubation

44 measurements ▼

Database ID

UniProt: A0A1Q2T258 ↗

Sequence Annotation

Explicit - Provided GenBank Accession Number

Protein Source

Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (44 measurements)

44 measurements — page 3 of 3

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Stability - Incubation	Activity after incubation (10 minutes at 25°C), measured by absorbance spectrophotometry NADH absorbance measurement in a coupled reaction with TIM and GPDH, with TIM converting G3P into DHAP, and GPDH reducing DHAP, 340 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	40%	100	99	%	Incubation: 10 mM Tris-HCl buffer, 2M NaCl, Assay: 100 mM citrate-Na citrate buffer, 1 M NaCl, 0.15 mM NADH, 8 U/ml triose-phosphate isomerase (rabbit muscle), 2.5 U/ml glycerol-3-phosphate dehydrogenase (rabbit muscle)	Incubation: 8, Assay: 6.4	Incubation: 25°C, Assay: 37°C	2 mM 2-Deoxyribose-5-phosphate	Acetaldehyde , Glyceraldehyde 3-phosphate	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Clear about assay in aqueous phase
Stability - Incubation	Activity after incubation (10 minutes at 25°C), measured by absorbance spectrophotometry NADH absorbance measurement in a coupled reaction with TIM and GPDH, with TIM converting G3P into DHAP, and GPDH reducing DHAP, 340 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	50%	100	89	%	Incubation: 10 mM Tris-HCl buffer, 2M NaCl, Assay: 100 mM citrate-Na citrate buffer, 1 M NaCl, 0.15 mM NADH, 8 U/ml triose-phosphate isomerase (rabbit muscle), 2.5 U/ml glycerol-3-phosphate dehydrogenase (rabbit muscle)	Incubation: 8, Assay: 6.4	Incubation: 25°C, Assay: 37°C	2 mM 2-Deoxyribose-5-phosphate	Acetaldehyde , Glyceraldehyde 3-phosphate	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Clear about assay in aqueous phase
Stability - Incubation	Activity after incubation (10 minutes at 25°C), measured by absorbance spectrophotometry NADH absorbance measurement in a coupled reaction with TIM and GPDH, with TIM converting G3P into DHAP, and GPDH reducing DHAP, 340 nm) in aqueous phase	Methanol	20%	100	108	%	Incubation: 10 mM Tris-HCl buffer, 2M NaCl, Assay: 100 mM citrate-Na citrate buffer, 1 M NaCl, 0.15 mM NADH, 8 U/ml triose-phosphate isomerase (rabbit muscle), 2.5 U/ml glycerol-3-phosphate dehydrogenase (rabbit muscle)	Incubation: 8, Assay: 6.4	Incubation: 25°C, Assay: 37°C	2 mM 2-Deoxyribose-5-phosphate	Acetaldehyde , Glyceraldehyde 3-phosphate	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Clear about assay in aqueous phase
Stability - Incubation	Activity after incubation (20 minutes at 25°C), measured by absorbance spectrophotometry NADH absorbance measurement in a coupled reaction with TIM and GPDH, with TIM converting G3P into DHAP, and GPDH reducing DHAP, 340 nm) in aqueous phase	Methanol	20%	100	98	%	Incubation: 10 mM Tris-HCl buffer, 2M NaCl, Assay: 100 mM citrate-Na citrate buffer, 1 M NaCl, 0.15 mM NADH, 8 U/ml triose-phosphate isomerase (rabbit muscle), 2.5 U/ml glycerol-3-phosphate dehydrogenase (rabbit muscle)	Incubation: 8, Assay: 6.4	Incubation: 25°C, Assay: 37°C	2 mM 2-Deoxyribose-5-phosphate	Acetaldehyde , Glyceraldehyde 3-phosphate	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Clear about assay in aqueous phase

Stability - Incubation	Activity after incubation (20 minutes at 25°C), measured by absorbance spectrophotometry NADH absorbance measurement in a coupled reaction with TIM and GPDH, with TIM converting G3P into DHAP, and GPDH reducing DHAP, 340 nm) in aqueous phase	Acetonitrile	50%	100	83	%	Incubation: 10 mM Tris-HCl buffer, 2M NaCl, Assay: 100 mM citrate-Na citrate buffer, 1 M NaCl, 0.15 mM NADH, 8 U/ml triose-phosphate isomerase (rabbit muscle), 2.5 U/ml glycerol-3-phosphate dehydrogenase (rabbit muscle)	Incubation: 8, Assay: 6.4	Incubation: 25°C, Assay: 37°C	2 mM 2-Deoxyribose-5-phosphate	Acetaldehyde, Glyceraldehyde 3-phosphate	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Clear about assay in aqueous phase
Stability - Incubation	Activity after incubation (40 minutes at 25°C), measured by absorbance spectrophotometry NADH absorbance measurement in a coupled reaction with TIM and GPDH, with TIM converting G3P into DHAP, and GPDH reducing DHAP, 340 nm) in aqueous phase	Methanol	20%	100	116	%	Incubation: 10 mM Tris-HCl buffer, 2M NaCl, Assay: 100 mM citrate-Na citrate buffer, 1 M NaCl, 0.15 mM NADH, 8 U/ml triose-phosphate isomerase (rabbit muscle), 2.5 U/ml glycerol-3-phosphate dehydrogenase (rabbit muscle)	Incubation: 8, Assay: 6.4	Incubation: 25°C, Assay: 37°C	2 mM 2-Deoxyribose-5-phosphate	Acetaldehyde, Glyceraldehyde 3-phosphate	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Clear about assay in aqueous phase
Stability - Incubation	Activity after incubation (50 minutes at 25°C), measured by absorbance spectrophotometry NADH absorbance measurement in a coupled reaction with TIM and GPDH, with TIM converting G3P into DHAP, and GPDH reducing DHAP, 340 nm) in aqueous phase	Methanol	20%	100	117	%	Incubation: 10 mM Tris-HCl buffer, 2M NaCl, Assay: 100 mM citrate-Na citrate buffer, 1 M NaCl, 0.15 mM NADH, 8 U/ml triose-phosphate isomerase (rabbit muscle), 2.5 U/ml glycerol-3-phosphate dehydrogenase (rabbit muscle)	Incubation: 8, Assay: 6.4	Incubation: 25°C, Assay: 37°C	2 mM 2-Deoxyribose-5-phosphate	Acetaldehyde, Glyceraldehyde 3-phosphate	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Clear about assay in aqueous phase
Stability - Incubation	Activity after incubation (60 minutes at 25°C), measured by absorbance spectrophotometry NADH absorbance measurement in a coupled reaction with TIM and GPDH, with TIM converting G3P into DHAP, and GPDH reducing DHAP, 340 nm) in aqueous phase	Methanol	20%	100	112	%	Incubation: 10 mM Tris-HCl buffer, 2M NaCl, Assay: 100 mM citrate-Na citrate buffer, 1 M NaCl, 0.15 mM NADH, 8 U/ml triose-phosphate isomerase (rabbit muscle), 2.5 U/ml glycerol-3-phosphate dehydrogenase (rabbit muscle)	Incubation: 8, Assay: 6.4	Incubation: 25°C, Assay: 37°C	2 mM 2-Deoxyribose-5-phosphate	Acetaldehyde, Glyceraldehyde 3-phosphate	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Clear about assay in aqueous phase
Stability - Incubation	Activity after incubation (10 minutes at 25°C), measured by absorbance spectrophotometry NADH absorbance measurement in a coupled reaction with TIM and GPDH, with TIM converting G3P into DHAP, and GPDH reducing DHAP, 340 nm) in aqueous phase	Ethanol	20%	100	92	%	Incubation: 10 mM Tris-HCl buffer, 2M NaCl, Assay: 100 mM citrate-Na citrate buffer, 1 M NaCl, 0.15 mM NADH, 8 U/ml triose-phosphate isomerase (rabbit muscle), 2.5 U/ml glycerol-3-phosphate dehydrogenase (rabbit muscle)	Incubation: 8, Assay: 6.4	Incubation: 25°C, Assay: 37°C	2 mM 2-Deoxyribose-5-phosphate	Acetaldehyde, Glyceraldehyde 3-phosphate	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Clear about assay in aqueous phase
Stability - Incubation	Activity after incubation (20 minutes at 25°C), measured by absorbance spectrophotometry NADH absorbance measurement in a coupled reaction with TIM and GPDH, with TIM converting G3P into DHAP, and GPDH reducing DHAP, 340 nm) in aqueous phase	Ethanol	20%	100	92	%	Incubation: 10 mM Tris-HCl buffer, 2M NaCl, Assay: 100 mM citrate-Na citrate buffer, 1 M NaCl, 0.15 mM NADH, 8 U/ml triose-phosphate isomerase (rabbit muscle), 2.5 U/ml glycerol-3-phosphate dehydrogenase (rabbit muscle)	Incubation: 8, Assay: 6.4	Incubation: 25°C, Assay: 37°C	2 mM 2-Deoxyribose-5-phosphate	Acetaldehyde, Glyceraldehyde 3-phosphate	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Clear about assay in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry NADH absorbance measurement in a coupled reaction with TIM and GPDH, with TIM converting G3P into DHAP, and GPDH reducing DHAP, 340 nm) in aqueous phase	Ethanol	20%	100	100	%	Incubation: 10 mM Tris-HCl buffer, 2M NaCl, Assay: 100 mM citrate-Na citrate buffer, 1 M NaCl, 0.15 mM NADH, 8 U/ml triose-phosphate isomerase (rabbit muscle), 2.5 U/ml glycerol-3-phosphate dehydrogenase (rabbit muscle)	Incubation: 8, Assay: 6.4	Incubation: 25°C, Assay: 37°C	2 mM 2-Deoxyribose-5-phosphate	Acetaldehyde, Glyceraldehyde 3-phosphate	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Clear about assay in aqueous phase
Stability - Incubation	Activity after incubation (40 minutes at 25°C), measured by absorbance spectrophotometry NADH absorbance measurement in a coupled reaction with TIM and GPDH, with TIM converting G3P into DHAP, and GPDH reducing DHAP, 340 nm) in aqueous phase	Ethanol	20%	100	111	%	Incubation: 10 mM Tris-HCl buffer, 2M NaCl, Assay: 100 mM citrate-Na citrate buffer, 1 M NaCl, 0.15 mM NADH, 8 U/ml triose-phosphate isomerase (rabbit muscle), 2.5 U/ml glycerol-3-phosphate dehydrogenase (rabbit muscle)	Incubation: 8, Assay: 6.4	Incubation: 25°C, Assay: 37°C	2 mM 2-Deoxyribose-5-phosphate	Acetaldehyde, Glyceraldehyde 3-phosphate	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Clear about assay in aqueous phase
Stability - Incubation	Activity after incubation (50 minutes at 25°C), measured by absorbance spectrophotometry NADH absorbance measurement in a coupled reaction with TIM and GPDH, with TIM converting G3P into DHAP, and GPDH reducing DHAP, 340 nm) in aqueous phase	Ethanol	20%	100	115	%	Incubation: 10 mM Tris-HCl buffer, 2M NaCl, Assay: 100 mM citrate-Na citrate buffer, 1 M NaCl, 0.15 mM NADH, 8 U/ml triose-phosphate isomerase (rabbit muscle), 2.5 U/ml glycerol-3-phosphate dehydrogenase (rabbit muscle)	Incubation: 8, Assay: 6.4	Incubation: 25°C, Assay: 37°C	2 mM 2-Deoxyribose-5-phosphate	Acetaldehyde, Glyceraldehyde 3-phosphate	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Clear about assay in aqueous phase
Stability - Incubation	Activity after incubation (60 minutes at 25°C), measured by absorbance spectrophotometry NADH absorbance measurement in a coupled reaction with TIM and GPDH, with TIM converting G3P into DHAP, and GPDH reducing DHAP, 340 nm) in aqueous phase	Ethanol	20%	100	114	%	Incubation: 10 mM Tris-HCl buffer, 2M NaCl, Assay: 100 mM citrate-Na citrate buffer, 1 M NaCl, 0.15 mM NADH, 8 U/ml triose-phosphate isomerase (rabbit muscle), 2.5 U/ml glycerol-3-phosphate dehydrogenase (rabbit muscle)	Incubation: 8, Assay: 6.4	Incubation: 25°C, Assay: 37°C	2 mM 2-Deoxyribose-5-phosphate	Acetaldehyde, Glyceraldehyde 3-phosphate	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Clear about assay in aqueous phase
Stability - Incubation	Activity after incubation (10 minutes at 25°C), measured by absorbance spectrophotometry NADH absorbance measurement in a coupled reaction with TIM and GPDH, with TIM converting G3P into DHAP, and GPDH reducing DHAP, 340 nm) in aqueous phase	Acetonitrile	50%	100	78	%	Incubation: 10 mM Tris-HCl buffer, 2M NaCl, Assay: 100 mM citrate-Na citrate buffer, 1 M NaCl, 0.15 mM NADH, 8 U/ml triose-phosphate isomerase (rabbit muscle), 2.5 U/ml glycerol-3-phosphate dehydrogenase (rabbit muscle)	Incubation: 8, Assay: 6.4	Incubation: 25°C, Assay: 37°C	2 mM 2-Deoxyribose-5-phosphate	Acetaldehyde, Glyceraldehyde 3-phosphate	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Clear about assay in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry NADH absorbance measurement in a coupled reaction with TIM and GPDH, with TIM converting G3P into DHAP, and GPDH reducing DHAP, 340 nm) in aqueous phase	Methanol	20%	100	109	%	Incubation: 10 mM Tris-HCl buffer, 2M NaCl, Assay: 100 mM citrate-Na citrate buffer, 1 M NaCl, 0.15 mM NADH, 8 U/ml triose-phosphate isomerase (rabbit muscle), 2.5 U/ml glycerol-3-phosphate dehydrogenase (rabbit muscle)	Incubation: 8, Assay: 6.4	Incubation: 25°C, Assay: 37°C	2 mM 2-Deoxyribose-5-phosphate	Acetaldehyde, Glyceraldehyde 3-phosphate	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Clear about assay in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry NADH absorbance measurement in a coupled reaction with TIM and GPDH, with TIM converting G3P into DHAP, and GPDH reducing DHAP, 340 nm) in aqueous phase	Acetonitrile	50%	100	97	%	Incubation: 10 mM Tris-HCl buffer, 2M NaCl, Assay: 100 mM citrate-Na citrate buffer, 1 M NaCl, 0.15 mM NADH, 8 U/ml triose-phosphate isomerase (rabbit muscle), 2.5 U/ml glycerol-3-phosphate dehydrogenase (rabbit muscle)	Incubation: 8, Assay: 6.4	Incubation: 25°C, Assay: 37°C	2 mM 2-Deoxyribose-5-phosphate	Acetaldehyde, Glyceraldehyde 3-phosphate	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Clear about assay in aqueous phase
Stability - Incubation	Activity after incubation (40 minutes at 25°C), measured by absorbance spectrophotometry NADH absorbance measurement in a coupled reaction with TIM and GPDH, with TIM converting G3P into DHAP, and GPDH reducing DHAP, 340 nm) in aqueous phase	Acetonitrile	50%	100	102	%	Incubation: 10 mM Tris-HCl buffer, 2M NaCl, Assay: 100 mM citrate-Na citrate buffer, 1 M NaCl, 0.15 mM NADH, 8 U/ml triose-phosphate isomerase (rabbit muscle), 2.5 U/ml glycerol-3-phosphate dehydrogenase (rabbit muscle)	Incubation: 8, Assay: 6.4	Incubation: 25°C, Assay: 37°C	2 mM 2-Deoxyribose-5-phosphate	Acetaldehyde, Glyceraldehyde 3-phosphate	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Clear about assay in aqueous phase
Stability - Incubation	Activity after incubation (50 minutes at 25°C), measured by absorbance spectrophotometry NADH absorbance measurement in a coupled reaction with TIM and GPDH, with TIM converting G3P into DHAP, and GPDH reducing DHAP, 340 nm) in aqueous phase	Acetonitrile	50%	100	97	%	Incubation: 10 mM Tris-HCl buffer, 2M NaCl, Assay: 100 mM citrate-Na citrate buffer, 1 M NaCl, 0.15 mM NADH, 8 U/ml triose-phosphate isomerase (rabbit muscle), 2.5 U/ml glycerol-3-phosphate dehydrogenase (rabbit muscle)	Incubation: 8, Assay: 6.4	Incubation: 25°C, Assay: 37°C	2 mM 2-Deoxyribose-5-phosphate	Acetaldehyde, Glyceraldehyde 3-phosphate	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Clear about assay in aqueous phase
Stability - Incubation	Activity after incubation (60 minutes at 25°C), measured by absorbance spectrophotometry NADH absorbance measurement in a coupled reaction with TIM and GPDH, with TIM converting G3P into DHAP, and GPDH reducing DHAP, 340 nm) in aqueous phase	Acetonitrile	50%	100	65	%	Incubation: 10 mM Tris-HCl buffer, 2M NaCl, Assay: 100 mM citrate-Na citrate buffer, 1 M NaCl, 0.15 mM NADH, 8 U/ml triose-phosphate isomerase (rabbit muscle), 2.5 U/ml glycerol-3-phosphate dehydrogenase (rabbit muscle)	Incubation: 8, Assay: 6.4	Incubation: 25°C, Assay: 37°C	2 mM 2-Deoxyribose-5-phosphate	Acetaldehyde, Glyceraldehyde 3-phosphate	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Clear about assay in aqueous phase
Stability - Incubation	Activity after incubation (10 minutes at 25°C), measured by absorbance spectrophotometry NADH absorbance measurement in a coupled reaction with TIM and GPDH, with TIM converting G3P into DHAP, and GPDH reducing DHAP, 340 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	50%	100	87	%	Incubation: 10 mM Tris-HCl buffer, 2M NaCl, Assay: 100 mM citrate-Na citrate buffer, 1 M NaCl, 0.15 mM NADH, 8 U/ml triose-phosphate isomerase (rabbit muscle), 2.5 U/ml glycerol-3-phosphate dehydrogenase (rabbit muscle)	Incubation: 8, Assay: 6.4	Incubation: 25°C, Assay: 37°C	2 mM 2-Deoxyribose-5-phosphate	Acetaldehyde, Glyceraldehyde 3-phosphate	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Clear about assay in aqueous phase
Stability - Incubation	Activity after incubation (20 minutes at 25°C), measured by absorbance spectrophotometry NADH absorbance measurement in a coupled reaction with TIM and GPDH, with TIM converting G3P into DHAP, and GPDH reducing DHAP, 340 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	50%	100	82	%	Incubation: 10 mM Tris-HCl buffer, 2M NaCl, Assay: 100 mM citrate-Na citrate buffer, 1 M NaCl, 0.15 mM NADH, 8 U/ml triose-phosphate isomerase (rabbit muscle), 2.5 U/ml glycerol-3-phosphate dehydrogenase (rabbit muscle)	Incubation: 8, Assay: 6.4	Incubation: 25°C, Assay: 37°C	2 mM 2-Deoxyribose-5-phosphate	Acetaldehyde, Glyceraldehyde 3-phosphate	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Clear about assay in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry NADH absorbance measurement in a coupled reaction with TIM and GPDH, with TIM converting G3P into DHAP, and GPDH reducing DHAP, 340 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	50%	100	96	%	Incubation: 10 mM Tris-HCl buffer, 2M NaCl, Assay: 100 mM citrate-Na citrate buffer, 1 M NaCl, 0.15 mM NADH, 8 U/ml triose-phosphate isomerase (rabbit muscle), 2.5 U/ml glycerol-3-phosphate dehydrogenase (rabbit muscle)	Incubation: 8, Assay: 6.4	Incubation: 25°C, Assay: 37°C	2 mM 2-Deoxyribose-5-phosphate	Acetaldehyde, Glyceraldehyde 3-phosphate	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Clear about assay in aqueous phase
Stability - Incubation	Activity after incubation (40 minutes at 25°C), measured by absorbance spectrophotometry NADH absorbance measurement in a coupled reaction with TIM and GPDH, with TIM converting G3P into DHAP, and GPDH reducing DHAP, 340 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	50%	100	104	%	Incubation: 10 mM Tris-HCl buffer, 2M NaCl, Assay: 100 mM citrate-Na citrate buffer, 1 M NaCl, 0.15 mM NADH, 8 U/ml triose-phosphate isomerase (rabbit muscle), 2.5 U/ml glycerol-3-phosphate dehydrogenase (rabbit muscle)	Incubation: 8, Assay: 6.4	Incubation: 25°C, Assay: 37°C	2 mM 2-Deoxyribose-5-phosphate	Acetaldehyde, Glyceraldehyde 3-phosphate	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Clear about assay in aqueous phase
Stability - Incubation	Activity after incubation (50 minutes at 25°C), measured by absorbance spectrophotometry NADH absorbance measurement in a coupled reaction with TIM and GPDH, with TIM converting G3P into DHAP, and GPDH reducing DHAP, 340 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	50%	100	101	%	Incubation: 10 mM Tris-HCl buffer, 2M NaCl, Assay: 100 mM citrate-Na citrate buffer, 1 M NaCl, 0.15 mM NADH, 8 U/ml triose-phosphate isomerase (rabbit muscle), 2.5 U/ml glycerol-3-phosphate dehydrogenase (rabbit muscle)	Incubation: 8, Assay: 6.4	Incubation: 25°C, Assay: 37°C	2 mM 2-Deoxyribose-5-phosphate	Acetaldehyde, Glyceraldehyde 3-phosphate	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Clear about assay in aqueous phase
Stability - Incubation	Activity after incubation (60 minutes at 25°C), measured by absorbance spectrophotometry NADH absorbance measurement in a coupled reaction with TIM and GPDH, with TIM converting G3P into DHAP, and GPDH reducing DHAP, 340 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	50%	100	97	%	Incubation: 10 mM Tris-HCl buffer, 2M NaCl, Assay: 100 mM citrate-Na citrate buffer, 1 M NaCl, 0.15 mM NADH, 8 U/ml triose-phosphate isomerase (rabbit muscle), 2.5 U/ml glycerol-3-phosphate dehydrogenase (rabbit muscle)	Incubation: 8, Assay: 6.4	Incubation: 25°C, Assay: 37°C	2 mM 2-Deoxyribose-5-phosphate	Acetaldehyde, Glyceraldehyde 3-phosphate	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Clear about assay in aqueous phase
Stability - Incubation	Activity after incubation (10 minutes at 25°C), measured by absorbance spectrophotometry NADH absorbance measurement in a coupled reaction with TIM and GPDH, with TIM converting G3P into DHAP, and GPDH reducing DHAP, 340 nm) in aqueous phase	Acetonitrile	20%	100	81	%	Incubation: 10 mM Tris-HCl buffer, 2M NaCl, Assay: 100 mM citrate-Na citrate buffer, 1 M NaCl, 0.15 mM NADH, 8 U/ml triose-phosphate isomerase (rabbit muscle), 2.5 U/ml glycerol-3-phosphate dehydrogenase (rabbit muscle)	Incubation: 8, Assay: 6.4	Incubation: 25°C, Assay: 37°C	2 mM 2-Deoxyribose-5-phosphate	Acetaldehyde, Glyceraldehyde 3-phosphate	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Clear about assay in aqueous phase
Stability - Incubation	Activity after incubation (10 minutes at 25°C), measured by absorbance spectrophotometry NADH absorbance measurement in a coupled reaction with TIM and GPDH, with TIM converting G3P into DHAP, and GPDH reducing DHAP, 340 nm) in aqueous phase	Ethanol	20%	100	87	%	Incubation: 10 mM Tris-HCl buffer, 2M NaCl, Assay: 100 mM citrate-Na citrate buffer, 1 M NaCl, 0.15 mM NADH, 8 U/ml triose-phosphate isomerase (rabbit muscle), 2.5 U/ml glycerol-3-phosphate dehydrogenase (rabbit muscle)	Incubation: 8, Assay: 6.4	Incubation: 25°C, Assay: 37°C	2 mM 2-Deoxyribose-5-phosphate	Acetaldehyde, Glyceraldehyde 3-phosphate	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Clear about assay in aqueous phase
Stability - Incubation	Activity after incubation (10 minutes at 25°C), measured by absorbance spectrophotometry NADH absorbance measurement in a coupled reaction with TIM and GPDH, with TIM converting G3P into DHAP, and GPDH reducing DHAP, 340 nm) in aqueous phase	Ethanol	30%	100	60	%	Incubation: 10 mM Tris-HCl buffer, 2M NaCl, Assay: 100 mM citrate-Na citrate buffer, 1 M NaCl, 0.15 mM NADH, 8 U/ml triose-phosphate isomerase (rabbit muscle), 2.5 U/ml glycerol-3-phosphate dehydrogenase (rabbit muscle)	Incubation: 8, Assay: 6.4	Incubation: 25°C, Assay: 37°C	2 mM 2-Deoxyribose-5-phosphate	Acetaldehyde, Glyceraldehyde 3-phosphate	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Clear about assay in aqueous phase
Stability - Incubation	Activity after incubation (10 minutes at 25°C), measured by absorbance spectrophotometry NADH absorbance measurement in a coupled reaction with TIM and GPDH, with TIM converting G3P into DHAP, and GPDH reducing DHAP, 340 nm) in aqueous phase	Ethanol	40%	100	46	%	Incubation: 10 mM Tris-HCl buffer, 2M NaCl, Assay: 100 mM citrate-Na citrate buffer, 1 M NaCl, 0.15 mM NADH, 8 U/ml triose-phosphate isomerase (rabbit muscle), 2.5 U/ml glycerol-3-phosphate dehydrogenase (rabbit muscle)	Incubation: 8, Assay: 6.4	Incubation: 25°C, Assay: 37°C	2 mM 2-Deoxyribose-5-phosphate	Acetaldehyde, Glyceraldehyde 3-phosphate	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Clear about assay in aqueous phase
Stability - Incubation	Activity after incubation (10 minutes at 25°C), measured by absorbance spectrophotometry NADH absorbance measurement in a coupled reaction with TIM and GPDH, with TIM converting G3P into DHAP, and GPDH reducing DHAP, 340 nm) in aqueous phase	Ethanol	50%	100	30	%	Incubation: 10 mM Tris-HCl buffer, 2M NaCl, Assay: 100 mM citrate-Na citrate buffer, 1 M NaCl, 0.15 mM NADH, 8 U/ml triose-phosphate isomerase (rabbit muscle), 2.5 U/ml glycerol-3-phosphate dehydrogenase (rabbit muscle)	Incubation: 8, Assay: 6.4	Incubation: 25°C, Assay: 37°C	2 mM 2-Deoxyribose-5-phosphate	Acetaldehyde, Glyceraldehyde 3-phosphate	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Clear about assay in aqueous phase
Stability - Incubation	Activity after incubation (10 minutes at 25°C), measured by absorbance spectrophotometry NADH absorbance measurement in a coupled reaction with TIM and GPDH, with TIM converting G3P into DHAP, and GPDH reducing DHAP, 340 nm) in aqueous phase	Methanol	10%	100	82	%	Incubation: 10 mM Tris-HCl buffer, 2M NaCl, Assay: 100 mM citrate-Na citrate buffer, 1 M NaCl, 0.15 mM NADH, 8 U/ml triose-phosphate isomerase (rabbit muscle), 2.5 U/ml glycerol-3-phosphate dehydrogenase (rabbit muscle)	Incubation: 8, Assay: 6.4	Incubation: 25°C, Assay: 37°C	2 mM 2-Deoxyribose-5-phosphate	Acetaldehyde, Glyceraldehyde 3-phosphate	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Clear about assay in aqueous phase
Stability - Incubation	Activity after incubation (10 minutes at 25°C), measured by absorbance spectrophotometry NADH absorbance measurement in a coupled reaction with TIM and GPDH, with TIM converting G3P into DHAP, and GPDH reducing DHAP, 340 nm) in aqueous phase	Methanol	20%	100	99	%	Incubation: 10 mM Tris-HCl buffer, 2M NaCl, Assay: 100 mM citrate-Na citrate buffer, 1 M NaCl, 0.15 mM NADH, 8 U/ml triose-phosphate isomerase (rabbit muscle), 2.5 U/ml glycerol-3-phosphate dehydrogenase (rabbit muscle)	Incubation: 8, Assay: 6.4	Incubation: 25°C, Assay: 37°C	2 mM 2-Deoxyribose-5-phosphate	Acetaldehyde, Glyceraldehyde 3-phosphate	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Clear about assay in aqueous phase
Stability - Incubation	Activity after incubation (10 minutes at 25°C), measured by absorbance spectrophotometry NADH absorbance measurement in a coupled reaction with TIM and GPDH, with TIM converting G3P into DHAP, and GPDH reducing DHAP, 340 nm) in aqueous phase	Methanol	30%	100	94	%	Incubation: 10 mM Tris-HCl buffer, 2M NaCl, Assay: 100 mM citrate-Na citrate buffer, 1 M NaCl, 0.15 mM NADH, 8 U/ml triose-phosphate isomerase (rabbit muscle), 2.5 U/ml glycerol-3-phosphate dehydrogenase (rabbit muscle)	Incubation: 8, Assay: 6.4	Incubation: 25°C, Assay: 37°C	2 mM 2-Deoxyribose-5-phosphate	Acetaldehyde, Glyceraldehyde 3-phosphate	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Clear about assay in aqueous phase
Stability - Incubation	Activity after incubation (10 minutes at 25°C), measured by absorbance spectrophotometry NADH absorbance measurement in a coupled reaction with TIM and GPDH, with TIM converting G3P into DHAP, and GPDH reducing DHAP, 340 nm) in aqueous phase	Methanol	40%	100	60	%	Incubation: 10 mM Tris-HCl buffer, 2M NaCl, Assay: 100 mM citrate-Na citrate buffer, 1 M NaCl, 0.15 mM NADH, 8 U/ml triose-phosphate isomerase (rabbit muscle), 2.5 U/ml glycerol-3-phosphate dehydrogenase (rabbit muscle)	Incubation: 8, Assay: 6.4	Incubation: 25°C, Assay: 37°C	2 mM 2-Deoxyribose-5-phosphate	Acetaldehyde, Glyceraldehyde 3-phosphate	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Clear about assay in aqueous phase
Stability - Incubation	Activity after incubation (10 minutes at 25°C), measured by absorbance spectrophotometry NADH absorbance measurement in a coupled reaction with TIM and GPDH, with TIM converting G3P into DHAP, and GPDH reducing DHAP, 340 nm) in aqueous phase	Methanol	50%	100	23	%	Incubation: 10 mM Tris-HCl buffer, 2M NaCl, Assay: 100 mM citrate-Na citrate buffer, 1 M NaCl, 0.15 mM NADH, 8 U/ml triose-phosphate isomerase (rabbit muscle), 2.5 U/ml glycerol-3-phosphate dehydrogenase (rabbit muscle)	Incubation: 8, Assay: 6.4	Incubation: 25°C, Assay: 37°C	2 mM 2-Deoxyribose-5-phosphate	Acetaldehyde, Glyceraldehyde 3-phosphate	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Clear about assay in aqueous phase
Stability - Incubation	Activity after incubation (10 minutes at 25°C), measured by absorbance spectrophotometry NADH absorbance measurement in a coupled reaction with TIM and GPDH, with TIM converting G3P into DHAP, and GPDH reducing DHAP, 340 nm) in aqueous phase	Acetonitrile	10%	100	96	%	Incubation: 10 mM Tris-HCl buffer, 2M NaCl, Assay: 100 mM citrate-Na citrate buffer, 1 M NaCl, 0.15 mM NADH, 8 U/ml triose-phosphate isomerase (rabbit muscle), 2.5 U/ml glycerol-3-phosphate dehydrogenase (rabbit muscle)	Incubation: 8, Assay: 6.4	Incubation: 25°C, Assay: 37°C	2 mM 2-Deoxyribose-5-phosphate	Acetaldehyde, Glyceraldehyde 3-phosphate	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Clear about assay in aqueous phase
Stability - Incubation	Activity after incubation (10 minutes at 25°C), measured by absorbance spectrophotometry NADH absorbance measurement in a coupled reaction with TIM and GPDH, with TIM converting G3P into DHAP, and GPDH reducing DHAP, 340 nm) in aqueous phase	Ethanol	10%	100	86	%	Incubation: 10 mM Tris-HCl buffer, 2M NaCl, Assay: 100 mM citrate-Na citrate buffer, 1 M NaCl, 0.15 mM NADH, 8 U/ml triose-phosphate isomerase (rabbit muscle), 2.5 U/ml glycerol-3-phosphate dehydrogenase (rabbit muscle)	Incubation: 8, Assay: 6.4	Incubation: 25°C, Assay: 37°C	2 mM 2-Deoxyribose-5-phosphate	Acetaldehyde, Glyceraldehyde 3-phosphate	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Clear about assay in aqueous phase
Stability - Incubation	Activity after incubation (10 minutes at 25°C), measured by absorbance spectrophotometry NADH absorbance measurement in a coupled reaction with TIM and GPDH, with TIM converting G3P into DHAP, and GPDH reducing DHAP, 340 nm) in aqueous phase	Acetonitrile	30%	100	85	%	Incubation: 10 mM Tris-HCl buffer, 2M NaCl, Assay: 100 mM citrate-Na citrate buffer, 1 M NaCl, 0.15 mM NADH, 8 U/ml triose-phosphate isomerase (rabbit muscle), 2.5 U/ml glycerol-3-phosphate dehydrogenase (rabbit muscle)	Incubation: 8, Assay: 6.4	Incubation: 25°C, Assay: 37°C	2 mM 2-Deoxyribose-5-phosphate	Acetaldehyde, Glyceraldehyde 3-phosphate	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Clear about assay in aqueous phase
Stability - Incubation	Activity after incubation (10 minutes at 25°C), measured by absorbance spectrophotometry NADH absorbance measurement in a coupled reaction with TIM and GPDH, with TIM converting G3P into DHAP, and GPDH reducing DHAP, 340 nm) in aqueous phase	Acetonitrile	40%	100	83	%	Incubation: 10 mM Tris-HCl buffer, 2M NaCl, Assay: 100 mM citrate-Na citrate buffer, 1 M NaCl, 0.15 mM NADH, 8 U/ml triose-phosphate isomerase (rabbit muscle), 2.5 U/ml glycerol-3-phosphate dehydrogenase (rabbit muscle)	Incubation: 8, Assay: 6.4	Incubation: 25°C, Assay: 37°C	2 mM 2-Deoxyribose-5-phosphate	Acetaldehyde, Glyceraldehyde 3-phosphate	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Clear about assay in aqueous phase
Stability - Incubation	Activity after incubation (10 minutes at 25°C), measured by absorbance spectrophotometry NADH absorbance measurement in a coupled reaction with TIM and GPDH, with TIM converting G3P into DHAP, and GPDH reducing DHAP, 340 nm) in aqueous phase	Acetonitrile	50%	100	84	%	Incubation: 10 mM Tris-HCl buffer, 2M NaCl, Assay: 100 mM citrate-Na citrate buffer, 1 M NaCl, 0.15 mM NADH, 8 U/ml triose-phosphate isomerase (rabbit muscle), 2.5 U/ml glycerol-3-phosphate dehydrogenase (rabbit muscle)	Incubation: 8, Assay: 6.4	Incubation: 25°C, Assay: 37°C	2 mM 2-Deoxyribose-5-phosphate	Acetaldehyde, Glyceraldehyde 3-phosphate	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Clear about assay in aqueous phase
Stability - Incubation	Activity after incubation (10 minutes at 25°C), measured by absorbance spectrophotometry NADH absorbance measurement in a coupled reaction with TIM and GPDH, with TIM converting G3P into DHAP, and GPDH reducing DHAP, 340 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	10%	100	94	%	Incubation: 10 mM Tris-HCl buffer, 2M NaCl, Assay: 100 mM citrate-Na citrate buffer, 1 M NaCl, 0.15 mM NADH, 8 U/ml triose-phosphate isomerase (rabbit muscle), 2.5 U/ml glycerol-3-phosphate dehydrogenase (rabbit muscle)	Incubation: 8, Assay: 6.4	Incubation: 25°C, Assay: 37°C	2 mM 2-Deoxyribose-5-phosphate	Acetaldehyde, Glyceraldehyde 3-phosphate	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Clear about assay in aqueous phase
Stability - Incubation	Activity after incubation (10 minutes at 25°C), measured by absorbance spectrophotometry NADH absorbance measurement in a coupled reaction with TIM and GPDH, with TIM converting G3P into DHAP, and GPDH reducing DHAP, 340 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	20%	100	96	%	Incubation: 10 mM Tris-HCl buffer, 2M NaCl, Assay: 100 mM citrate-Na citrate buffer, 1 M NaCl, 0.15 mM NADH, 8 U/ml triose-phosphate isomerase (rabbit muscle), 2.5 U/ml glycerol-3-phosphate dehydrogenase (rabbit muscle)	Incubation: 8, Assay: 6.4	Incubation: 25°C, Assay: 37°C	2 mM 2-Deoxyribose-5-phosphate	Acetaldehyde, Glyceraldehyde 3-phosphate	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Clear about assay in aqueous phase
Stability - Incubation	Activity after incubation (10 minutes at 25°C), measured by absorbance spectrophotometry NADH absorbance measurement in a coupled reaction with TIM and GPDH, with TIM converting G3P into DHAP, and GPDH reducing DHAP, 340 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	30%	100	98	%	Incubation: 10 mM Tris-HCl buffer, 2M NaCl, Assay: 100 mM citrate-Na citrate buffer, 1 M NaCl, 0.15 mM NADH, 8 U/ml triose-phosphate isomerase (rabbit muscle), 2.5 U/ml glycerol-3-phosphate dehydrogenase (rabbit muscle)	Incubation: 8, Assay: 6.4	Incubation: 25°C, Assay: 37°C	2 mM 2-Deoxyribose-5-phosphate	Acetaldehyde, Glyceraldehyde 3-phosphate	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Clear about assay in aqueous phase
Stability - Incubation	Activity after incubation (10 minutes at 25°C), measured by absorbance spectrophotometry NADH absorbance measurement in a coupled reaction with TIM and GPDH, with TIM converting G3P into DHAP, and GPDH reducing DHAP, 340 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	40%	100	99	%	Incubation: 10 mM Tris-HCl buffer, 2M NaCl, Assay: 100 mM citrate-Na citrate buffer, 1 M NaCl, 0.15 mM NADH, 8 U/ml triose-phosphate isomerase (rabbit muscle), 2.5 U/ml glycerol-3-phosphate dehydrogenase (rabbit muscle)	Incubation: 8, Assay: 6.4	Incubation: 25°C, Assay: 37°C	2 mM 2-Deoxyribose-5-phosphate	Acetaldehyde, Glyceraldehyde 3-phosphate	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Clear about assay in aqueous phase
Stability - Incubation	Activity after incubation (10 minutes at 25°C), measured by absorbance spectrophotometry NADH absorbance measurement in a coupled reaction with TIM and GPDH, with TIM converting G3P into DHAP, and GPDH reducing DHAP, 340 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	50%	100	89	%	Incubation: 10 mM Tris-HCl buffer, 2M NaCl, Assay: 100 mM citrate-Na citrate buffer, 1 M NaCl, 0.15 mM NADH, 8 U/ml triose-phosphate isomerase (rabbit muscle), 2.5 U/ml glycerol-3-phosphate dehydrogenase (rabbit muscle)	Incubation: 8, Assay: 6.4	Incubation: 25°C, Assay: 37°C	2 mM 2-Deoxyribose-5-phosphate	Acetaldehyde, Glyceraldehyde 3-phosphate	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Clear about assay in aqueous phase
Stability - Incubation	Activity after incubation (10 minutes at 25°C), measured by absorbance spectrophotometry NADH absorbance measurement in a coupled reaction with TIM and GPDH, with TIM converting G3P into DHAP, and GPDH reducing DHAP, 340 nm) in aqueous phase	Methanol	20%	100	108	%	Incubation: 10 mM Tris-HCl buffer, 2M NaCl, Assay: 100 mM citrate-Na citrate buffer, 1 M NaCl, 0.15 mM NADH, 8 U/ml triose-phosphate isomerase (rabbit muscle), 2.5 U/ml glycerol-3-phosphate dehydrogenase (rabbit muscle)	Incubation: 8, Assay: 6.4	Incubation: 25°C, Assay: 37°C	2 mM 2-Deoxyribose-5-phosphate	Acetaldehyde, Glyceraldehyde 3-phosphate	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Clear about assay in aqueous phase
Stability - Incubation	Activity after incubation (20 minutes at 25°C), measured by absorbance spectrophotometry NADH absorbance measurement in a coupled reaction with TIM and GPDH, with TIM converting G3P into DHAP, and GPDH reducing DHAP, 340 nm) in aqueous phase	Methanol	20%	100	98	%	Incubation: 10 mM Tris-HCl buffer, 2M NaCl, Assay: 100 mM citrate-Na citrate buffer, 1 M NaCl, 0.15 mM NADH, 8 U/ml triose-phosphate isomerase (rabbit muscle), 2.5 U/ml glycerol-3-phosphate dehydrogenase (rabbit muscle)	Incubation: 8, Assay: 6.4	Incubation: 25°C, Assay: 37°C	2 mM 2-Deoxyribose-5-phosphate	Acetaldehyde, Glyceraldehyde 3-phosphate	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Clear about assay in aqueous phase

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Visualization : Stability — Incubation

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