Esterase (gene EstLiu) from Zunongwangia profunda

Enzyme Description

Species

Zunongwangia profunda ↗

Extremophile

Yes psychrophilic (and "halo-tolerant" enzyme)

EC Number

3.1.1.1 ↗

Sequence

Length: 293 amino acids

MKTKIIAFLGLFVAVFQLSAQDEVIKLYDGKAPGSEDWNWEEAQKFSDLFKTEVVYNVTDPSLLVFKPETPNGTAIIIAPGGGFQTLSINREGIDLAKELASKGVTAFVLKYRLVHSKTDSPAKELMESLKDRADFDAKRVKIKKLAGQDMQAALKYVRDNAAQFNIAKDKVGVIGFSAGATVALESVLRSASKATLPNFAASLYGGPSDDIMAQDIPKEIPLFIAAASDDQLKLAPKSIALYNKWLAAGYPVELHMYAKGGHGFGMGTQNLPVDTWYKRYEDWLTQYKYLNK

Mohammad Asadur Rahman et al. (2016) — Characterization of a novel cold active and salt tolerant esterase from Zunongwangia profunda

Enzyme and Microbial Technology · doi:10.1016/j.enzmictec.2015.12.013 ↗ · Stability - Incubation

42 measurements ▼

Database ID

UniProt: D5BL39 ↗

Sequence Annotation

Explicit - Provided

Protein Source

Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (42 measurements)

42 measurements — page 3 of 3

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Stability - Incubation	Activity after incubation (40 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Methanol	10%	100	95	%	Incubation: ?, Assay: 50 mM Tris-HCl	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 30°C	0.5 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (40 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Methanol	20%	100	74	%	Incubation: ?, Assay: 50 mM Tris-HCl	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 30°C	0.5 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase

Stability - Incubation	Activity after incubation (40 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Acetonitrile	20%	100	70	%	Incubation: ?, Assay: 50 mM Tris-HCl	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 30°C	0.5 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (40 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Methanol	40%	100	62	%	Incubation: ?, Assay: 50 mM Tris-HCl	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 30°C	0.5 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (40 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Methanol	50%	100	39	%	Incubation: ?, Assay: 50 mM Tris-HCl	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 30°C	0.5 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (40 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Ethylene Glycol	5%	100	120	%	Incubation: ?, Assay: 50 mM Tris-HCl	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 30°C	0.5 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (40 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Ethylene Glycol	10%	100	101	%	Incubation: ?, Assay: 50 mM Tris-HCl	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 30°C	0.5 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (40 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Ethylene Glycol	20%	100	97	%	Incubation: ?, Assay: 50 mM Tris-HCl	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 30°C	0.5 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (40 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Ethylene Glycol	30%	100	80	%	Incubation: ?, Assay: 50 mM Tris-HCl	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 30°C	0.5 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (40 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Ethylene Glycol	40%	100	98	%	Incubation: ?, Assay: 50 mM Tris-HCl	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 30°C	0.5 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (40 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Ethylene Glycol	50%	100	79	%	Incubation: ?, Assay: 50 mM Tris-HCl	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 30°C	0.5 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (40 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Acetonitrile	5%	100	96	%	Incubation: ?, Assay: 50 mM Tris-HCl	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 30°C	0.5 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (40 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Acetonitrile	10%	100	91	%	Incubation: ?, Assay: 50 mM Tris-HCl	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 30°C	0.5 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (40 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Methanol	30%	100	89	%	Incubation: ?, Assay: 50 mM Tris-HCl	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 30°C	0.5 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (40 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Acetonitrile	30%	100	26	%	Incubation: ?, Assay: 50 mM Tris-HCl	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 30°C	0.5 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (40 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Acetonitrile	40%	100	28	%	Incubation: ?, Assay: 50 mM Tris-HCl	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 30°C	0.5 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (40 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Acetonitrile	50%	100	14	%	Incubation: ?, Assay: 50 mM Tris-HCl	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 30°C	0.5 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (40 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	1-Propanol	5%	100	103	%	Incubation: ?, Assay: 50 mM Tris-HCl	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 30°C	0.5 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (40 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	1-Propanol	10%	100	94	%	Incubation: ?, Assay: 50 mM Tris-HCl	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 30°C	0.5 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (40 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	1-Propanol	20%	100	64	%	Incubation: ?, Assay: 50 mM Tris-HCl	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 30°C	0.5 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (40 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	1-Propanol	30%	100	20	%	Incubation: ?, Assay: 50 mM Tris-HCl	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 30°C	0.5 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (40 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	1-Propanol	40%	100	9	%	Incubation: ?, Assay: 50 mM Tris-HCl	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 30°C	0.5 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (40 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	1-Propanol	50%	100	4	%	Incubation: ?, Assay: 50 mM Tris-HCl	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 30°C	0.5 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (40 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Isopropanol	50%	100	103	%	Incubation: ?, Assay: 50 mM Tris-HCl	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 30°C	0.5 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (40 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Ethanol	10%	100	114	%	Incubation: ?, Assay: 50 mM Tris-HCl	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 30°C	0.5 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (40 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Ethanol	20%	100	119	%	Incubation: ?, Assay: 50 mM Tris-HCl	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 30°C	0.5 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (40 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Ethanol	30%	100	102	%	Incubation: ?, Assay: 50 mM Tris-HCl	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 30°C	0.5 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (40 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Ethanol	40%	100	68	%	Incubation: ?, Assay: 50 mM Tris-HCl	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 30°C	0.5 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (40 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Ethanol	50%	100	81	%	Incubation: ?, Assay: 50 mM Tris-HCl	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 30°C	0.5 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (40 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Isopropanol	5%	100	133	%	Incubation: ?, Assay: 50 mM Tris-HCl	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 30°C	0.5 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (40 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Isopropanol	10%	100	120	%	Incubation: ?, Assay: 50 mM Tris-HCl	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 30°C	0.5 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (40 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Isopropanol	20%	100	153	%	Incubation: ?, Assay: 50 mM Tris-HCl	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 30°C	0.5 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (40 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Isopropanol	30%	100	145	%	Incubation: ?, Assay: 50 mM Tris-HCl	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 30°C	0.5 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (40 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Isopropanol	40%	100	138	%	Incubation: ?, Assay: 50 mM Tris-HCl	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 30°C	0.5 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (40 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Ethanol	5%	100	155	%	Incubation: ?, Assay: 50 mM Tris-HCl	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 30°C	0.5 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (40 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	5%	100	111	%	Incubation: ?, Assay: 50 mM Tris-HCl	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 30°C	0.5 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (40 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	10%	100	87	%	Incubation: ?, Assay: 50 mM Tris-HCl	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 30°C	0.5 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (40 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	20%	100	82	%	Incubation: ?, Assay: 50 mM Tris-HCl	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 30°C	0.5 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (40 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	30%	100	72	%	Incubation: ?, Assay: 50 mM Tris-HCl	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 30°C	0.5 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (40 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	40%	100	68	%	Incubation: ?, Assay: 50 mM Tris-HCl	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 30°C	0.5 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (40 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	50%	100	67	%	Incubation: ?, Assay: 50 mM Tris-HCl	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 30°C	0.5 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (40 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Methanol	5%	100	117	%	Incubation: ?, Assay: 50 mM Tris-HCl	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 30°C	0.5 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (40 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Methanol	10%	100	95	%	Incubation: ?, Assay: 50 mM Tris-HCl	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 30°C	0.5 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (40 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Methanol	20%	100	74	%	Incubation: ?, Assay: 50 mM Tris-HCl	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 30°C	0.5 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase

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Visualization : Stability — Incubation

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