Phenolic acid decarboxylase (gene blpad) from Bacillus licheniformis

Enzyme Description

Species

Extremophile

EC Number

4.2.1.- ↗

Sequence

Length: 166 amino acids

MNQDVKEFVGSHMIYTYENGWEYEIYIKNDHTIDYRIHSGMVGGRWVRDQKADIVKLTEGVYKVSWTEPTGTDVSLNFMPNEKRMHGIIFFPKWVHERPDITVCYQNDHIDLMEESREKYETYPKYVVPEFADITFIENAGIDNEDLISKAPYPGMTDDIRAGKRV

Hongfei Hu et al. (2014) — An organic solvent-tolerant phenolic acid decarboxylase from Bacillus licheniformis for the efficient bioconversion of hydroxycinnamic acids to vinyl phenol derivatives

Applied Microbiology and Biotechnology · doi:10.1007/s00253-014-6313-3 ↗ · Activity - Classical Stability - Incubation

45 measurements ▼

Database ID

UniProt: A0A0B4UFV2 ↗

Sequence Annotation

Explicit - Provided

Protein Source

Recombinant, host bacterium Escherichia coli BL21

Experimental Data (45 measurements)

45 measurements — page 1 of 3

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Activity - Classical	Activity measured by HPLC in the presence of organic solvent	n-Hexadecane	50%	100.0	127.77	%	160 mM Na₂HPO₄–citric acid buffer	6	37°C	5 mM Ferulic acid	4-Vinylguaiacol , Carbon Dioxide (CO2)	—	200 rpm	Classical aqueous control (in %)
Activity - Classical	Activity measured by HPLC in the presence of organic solvent	Toluene	50%	100.0	187.38	%	160 mM Na₂HPO₄–citric acid buffer	6	37°C	5 mM p-Coumaric acid	4-Vinylphenol , Carbon Dioxide (CO2)	—	200 rpm	Classical aqueous control (in %)
Activity - Classical	Activity measured by HPLC in the presence of organic solvent	1-Octanol	50%	100.0	71.89	%	160 mM Na₂HPO₄–citric acid buffer	6	37°C	5 mM p-Coumaric acid	4-Vinylphenol , Carbon Dioxide (CO2)	—	200 rpm	Classical aqueous control (in %)
Activity - Classical	Activity measured by HPLC in the presence of organic solvent	Cyclohexane	50%	100.0	85.61	%	160 mM Na₂HPO₄–citric acid buffer	6	37°C	5 mM p-Coumaric acid	4-Vinylphenol , Carbon Dioxide (CO2)	—	200 rpm	Classical aqueous control (in %)
Activity - Classical	Activity measured by HPLC in the presence of organic solvent	n-Hexane	50%	100.0	90.97	%	160 mM Na₂HPO₄–citric acid buffer	6	37°C	5 mM p-Coumaric acid	4-Vinylphenol , Carbon Dioxide (CO2)	—	200 rpm	Classical aqueous control (in %)
Activity - Classical	Activity measured by HPLC in the presence of organic solvent	n-Heptane	50%	100.0	94.53	%	160 mM Na₂HPO₄–citric acid buffer	6	37°C	5 mM p-Coumaric acid	4-Vinylphenol , Carbon Dioxide (CO2)	—	200 rpm	Classical aqueous control (in %)
Activity - Classical	Activity measured by HPLC in the presence of organic solvent	Petroleum Ether	50%	100.0	96.32	%	160 mM Na₂HPO₄–citric acid buffer	6	37°C	5 mM p-Coumaric acid	4-Vinylphenol , Carbon Dioxide (CO2)	—	200 rpm	Classical aqueous control (in %)
Activity - Classical	Activity measured by HPLC in the presence of organic solvent	n-Octane	50%	100.0	105.79	%	160 mM Na₂HPO₄–citric acid buffer	6	37°C	5 mM p-Coumaric acid	4-Vinylphenol , Carbon Dioxide (CO2)	—	200 rpm	Classical aqueous control (in %)
Activity - Classical	Activity measured by HPLC in the presence of organic solvent	n-Decane	50%	100.0	124.7	%	160 mM Na₂HPO₄–citric acid buffer	6	37°C	5 mM p-Coumaric acid	4-Vinylphenol , Carbon Dioxide (CO2)	—	200 rpm	Classical aqueous control (in %)
Activity - Classical	Activity measured by HPLC in the presence of organic solvent	n-Hexadecane	50%	100.0	100.3	%	160 mM Na₂HPO₄–citric acid buffer	6	37°C	5 mM p-Coumaric acid	4-Vinylphenol , Carbon Dioxide (CO2)	—	200 rpm	Classical aqueous control (in %)
Activity - Classical	Activity measured by HPLC in the presence of organic solvent	Toluene	50%	100.0	186.38	%	160 mM Na₂HPO₄–citric acid buffer	6	37°C	5 mM Ferulic acid	4-Vinylguaiacol , Carbon Dioxide (CO2)	—	200 rpm	Classical aqueous control (in %)
Activity - Classical	Activity measured by HPLC in the presence of organic solvent	1-Octanol	50%	100.0	70.15	%	160 mM Na₂HPO₄–citric acid buffer	6	37°C	5 mM Ferulic acid	4-Vinylguaiacol , Carbon Dioxide (CO2)	—	200 rpm	Classical aqueous control (in %)
Activity - Classical	Activity measured by HPLC in the presence of organic solvent	Cyclohexane	50%	100.0	214.7	%	160 mM Na₂HPO₄–citric acid buffer	6	37°C	5 mM Ferulic acid	4-Vinylguaiacol , Carbon Dioxide (CO2)	—	200 rpm	Classical aqueous control (in %)
Activity - Classical	Activity measured by HPLC in the presence of organic solvent	n-Hexane	50%	100.0	158.5	%	160 mM Na₂HPO₄–citric acid buffer	6	37°C	5 mM Ferulic acid	4-Vinylguaiacol , Carbon Dioxide (CO2)	—	200 rpm	Classical aqueous control (in %)
Activity - Classical	Activity measured by HPLC in the presence of organic solvent	n-Heptane	50%	100.0	119.64	%	160 mM Na₂HPO₄–citric acid buffer	6	37°C	5 mM Ferulic acid	4-Vinylguaiacol , Carbon Dioxide (CO2)	—	200 rpm	Classical aqueous control (in %)
Activity - Classical	Activity measured by HPLC in the presence of organic solvent	Petroleum Ether	50%	100.0	192.59	%	160 mM Na₂HPO₄–citric acid buffer	6	37°C	5 mM Ferulic acid	4-Vinylguaiacol , Carbon Dioxide (CO2)	—	200 rpm	Classical aqueous control (in %)
Activity - Classical	Activity measured by HPLC in the presence of organic solvent	n-Octane	50%	100.0	167.23	%	160 mM Na₂HPO₄–citric acid buffer	6	37°C	5 mM Ferulic acid	4-Vinylguaiacol , Carbon Dioxide (CO2)	—	200 rpm	Classical aqueous control (in %)
Activity - Classical	Activity measured by HPLC in the presence of organic solvent	n-Decane	50%	100.0	253.0	%	160 mM Na₂HPO₄–citric acid buffer	6	37°C	5 mM Ferulic acid	4-Vinylguaiacol , Carbon Dioxide (CO2)	—	200 rpm	Classical aqueous control (in %)
Stability - Incubation	Activity after incubation (12 hour at 25°C), measured by HPLC in aqueous phase	n-Decane	30%	100.0	92.79	%	Incubation: 160 mM Na₂HPO₄–citric acid buffer, Assay: 160 mM Na₂HPO₄–citric acid buffer	Incubation: 6, Assay: 6	Incubation: 37°C, Assay: 37°C	5 mM p-Coumaric acid	4-Vinylphenol , Carbon Dioxide (CO2)	—	Incubation: 200 rpm	Non-incubated control (in %), assay measured in aqueous phase \| Clear about assay in aqueous phase
Stability - Incubation	Activity after incubation (12 hour at 25°C), measured by HPLC in aqueous phase	n-Hexadecane	50%	100.0	91.86	%	Incubation: 160 mM Na₂HPO₄–citric acid buffer, Assay: 160 mM Na₂HPO₄–citric acid buffer	Incubation: 6, Assay: 6	Incubation: 37°C, Assay: 37°C	5 mM p-Coumaric acid	4-Vinylphenol , Carbon Dioxide (CO2)	—	Incubation: 200 rpm	Non-incubated control (in %), assay measured in aqueous phase \| Clear about assay in aqueous phase

Activity - Classical

Activity measured by HPLC in the presence of organic solvent

n-Hexadecane

50%

100.0

127.77

160 mM Na₂HPO₄–citric acid buffer

37°C

5 mM Ferulic acid

4-Vinylguaiacol, Carbon Dioxide (CO2)

—

200 rpm

Classical aqueous control (in %)

Activity - Classical

Activity measured by HPLC in the presence of organic solvent

Toluene

50%

100.0

187.38

160 mM Na₂HPO₄–citric acid buffer

37°C

5 mM p-Coumaric acid

4-Vinylphenol, Carbon Dioxide (CO2)

—

200 rpm

Classical aqueous control (in %)

Activity - Classical

Activity measured by HPLC in the presence of organic solvent

1-Octanol

50%

100.0

71.89

160 mM Na₂HPO₄–citric acid buffer

37°C

5 mM p-Coumaric acid

4-Vinylphenol, Carbon Dioxide (CO2)

—

200 rpm

Classical aqueous control (in %)

Activity - Classical

Activity measured by HPLC in the presence of organic solvent

Cyclohexane

50%

100.0

85.61

160 mM Na₂HPO₄–citric acid buffer

37°C

5 mM p-Coumaric acid

4-Vinylphenol, Carbon Dioxide (CO2)

—

200 rpm

Classical aqueous control (in %)

Activity - Classical

Activity measured by HPLC in the presence of organic solvent

n-Hexane

50%

100.0

90.97

160 mM Na₂HPO₄–citric acid buffer

37°C

5 mM p-Coumaric acid

4-Vinylphenol, Carbon Dioxide (CO2)

—

200 rpm

Classical aqueous control (in %)

Activity - Classical

Activity measured by HPLC in the presence of organic solvent

n-Heptane

50%

100.0

94.53

160 mM Na₂HPO₄–citric acid buffer

37°C

5 mM p-Coumaric acid

4-Vinylphenol, Carbon Dioxide (CO2)

—

200 rpm

Classical aqueous control (in %)

Activity - Classical

Activity measured by HPLC in the presence of organic solvent

Petroleum Ether

50%

100.0

96.32

160 mM Na₂HPO₄–citric acid buffer

37°C

5 mM p-Coumaric acid

4-Vinylphenol, Carbon Dioxide (CO2)

—

200 rpm

Classical aqueous control (in %)

Activity - Classical

Activity measured by HPLC in the presence of organic solvent

n-Octane

50%

100.0

105.79

160 mM Na₂HPO₄–citric acid buffer

37°C

5 mM p-Coumaric acid

4-Vinylphenol, Carbon Dioxide (CO2)

—

200 rpm

Classical aqueous control (in %)

Activity - Classical

Activity measured by HPLC in the presence of organic solvent

n-Decane

50%

100.0

124.7

160 mM Na₂HPO₄–citric acid buffer

37°C

5 mM p-Coumaric acid

4-Vinylphenol, Carbon Dioxide (CO2)

—

200 rpm

Classical aqueous control (in %)

Activity - Classical

Activity measured by HPLC in the presence of organic solvent

n-Hexadecane

50%

100.0

100.3

160 mM Na₂HPO₄–citric acid buffer

37°C

5 mM p-Coumaric acid

4-Vinylphenol, Carbon Dioxide (CO2)

—

200 rpm

Classical aqueous control (in %)

Activity - Classical

Activity measured by HPLC in the presence of organic solvent

Toluene

50%

100.0

186.38

160 mM Na₂HPO₄–citric acid buffer

37°C

5 mM Ferulic acid

4-Vinylguaiacol, Carbon Dioxide (CO2)

—

200 rpm

Classical aqueous control (in %)

Activity - Classical

Activity measured by HPLC in the presence of organic solvent

1-Octanol

50%

100.0

70.15

160 mM Na₂HPO₄–citric acid buffer

37°C

5 mM Ferulic acid

4-Vinylguaiacol, Carbon Dioxide (CO2)

—

200 rpm

Classical aqueous control (in %)

Activity - Classical

Activity measured by HPLC in the presence of organic solvent

Cyclohexane

50%

100.0

214.7

160 mM Na₂HPO₄–citric acid buffer

37°C

5 mM Ferulic acid

4-Vinylguaiacol, Carbon Dioxide (CO2)

—

200 rpm

Classical aqueous control (in %)

Activity - Classical

Activity measured by HPLC in the presence of organic solvent

n-Hexane

50%

100.0

158.5

160 mM Na₂HPO₄–citric acid buffer

37°C

5 mM Ferulic acid

4-Vinylguaiacol, Carbon Dioxide (CO2)

—

200 rpm

Classical aqueous control (in %)

Activity - Classical

Activity measured by HPLC in the presence of organic solvent

n-Heptane

50%

100.0

119.64

160 mM Na₂HPO₄–citric acid buffer

37°C

5 mM Ferulic acid

4-Vinylguaiacol, Carbon Dioxide (CO2)

—

200 rpm

Classical aqueous control (in %)

Activity - Classical

Activity measured by HPLC in the presence of organic solvent

Petroleum Ether

50%

100.0

192.59

160 mM Na₂HPO₄–citric acid buffer

37°C

5 mM Ferulic acid

4-Vinylguaiacol, Carbon Dioxide (CO2)

—

200 rpm

Classical aqueous control (in %)

Activity - Classical

Activity measured by HPLC in the presence of organic solvent

n-Octane

50%

100.0

167.23

160 mM Na₂HPO₄–citric acid buffer

37°C

5 mM Ferulic acid

4-Vinylguaiacol, Carbon Dioxide (CO2)

—

200 rpm

Classical aqueous control (in %)

Activity - Classical

Activity measured by HPLC in the presence of organic solvent

n-Decane

50%

100.0

253.0

160 mM Na₂HPO₄–citric acid buffer

37°C

5 mM Ferulic acid

4-Vinylguaiacol, Carbon Dioxide (CO2)

—

200 rpm

Classical aqueous control (in %)

Stability - Incubation

Activity after incubation (12 hour at 25°C), measured by HPLC in aqueous phase

n-Decane

30%

100.0

92.79

Incubation: 160 mM Na₂HPO₄–citric acid buffer, Assay: 160 mM Na₂HPO₄–citric acid buffer

Incubation: 6, Assay: 6

Incubation: 37°C, Assay: 37°C

5 mM p-Coumaric acid

4-Vinylphenol, Carbon Dioxide (CO2)

—

Incubation: 200 rpm

Non-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase

Stability - Incubation

Activity after incubation (12 hour at 25°C), measured by HPLC in aqueous phase

n-Hexadecane

50%

100.0

91.86

Incubation: 160 mM Na₂HPO₄–citric acid buffer, Assay: 160 mM Na₂HPO₄–citric acid buffer

Incubation: 6, Assay: 6

Incubation: 37°C, Assay: 37°C

5 mM p-Coumaric acid

4-Vinylphenol, Carbon Dioxide (CO2)

—

Incubation: 200 rpm

Non-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase

Stability - Incubation

Activity after incubation (12 hour at 25°C), measured by HPLC in aqueous phase

n-Hexadecane

30%

100.0

86.94

Incubation: 160 mM Na₂HPO₄–citric acid buffer, Assay: 160 mM Na₂HPO₄–citric acid buffer

Incubation: 6, Assay: 6

Incubation: 37°C, Assay: 37°C

5 mM p-Coumaric acid

4-Vinylphenol, Carbon Dioxide (CO2)

—

Incubation: 200 rpm

Non-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase

Stability - Incubation

Activity after incubation (12 hour at 25°C), measured by HPLC in aqueous phase

n-Hexadecane

20%

100.0

103.5

Incubation: 160 mM Na₂HPO₄–citric acid buffer, Assay: 160 mM Na₂HPO₄–citric acid buffer

Incubation: 6, Assay: 6

Incubation: 37°C, Assay: 37°C

5 mM p-Coumaric acid

4-Vinylphenol, Carbon Dioxide (CO2)

—

Incubation: 200 rpm

Non-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase

Stability - Incubation

Activity after incubation (12 hour at 25°C), measured by HPLC in aqueous phase

n-Decane

50%

100.0

42.15

Incubation: 160 mM Na₂HPO₄–citric acid buffer, Assay: 160 mM Na₂HPO₄–citric acid buffer

Incubation: 6, Assay: 6

Incubation: 37°C, Assay: 37°C

5 mM p-Coumaric acid

4-Vinylphenol, Carbon Dioxide (CO2)

—

Incubation: 200 rpm

Non-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase

Stability - Incubation

Activity after incubation (12 hour at 25°C), measured by HPLC in aqueous phase

n-Hexane

50%

100.0

99.57

Incubation: 160 mM Na₂HPO₄–citric acid buffer, Assay: 160 mM Na₂HPO₄–citric acid buffer

Incubation: 6, Assay: 6

Incubation: 37°C, Assay: 37°C

5 mM p-Coumaric acid

4-Vinylphenol, Carbon Dioxide (CO2)

—

Incubation: 200 rpm

Non-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase

Stability - Incubation

Activity after incubation (12 hour at 25°C), measured by HPLC in aqueous phase

Toluene

30%

100.0

126.22

Incubation: 160 mM Na₂HPO₄–citric acid buffer, Assay: 160 mM Na₂HPO₄–citric acid buffer

Incubation: 6, Assay: 6

Incubation: 37°C, Assay: 37°C

5 mM p-Coumaric acid

4-Vinylphenol, Carbon Dioxide (CO2)

—

Incubation: 200 rpm

Non-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase

Stability - Incubation

Activity after incubation (12 hour at 25°C), measured by HPLC in aqueous phase

Toluene

50%

100.0

128.47

Incubation: 160 mM Na₂HPO₄–citric acid buffer, Assay: 160 mM Na₂HPO₄–citric acid buffer

Incubation: 6, Assay: 6

Incubation: 37°C, Assay: 37°C

5 mM p-Coumaric acid

4-Vinylphenol, Carbon Dioxide (CO2)

—

Incubation: 200 rpm

Non-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase

Stability - Incubation

Activity after incubation (12 hour at 25°C), measured by HPLC in aqueous phase

1-Octanol

20%

100.0

106.45

Incubation: 160 mM Na₂HPO₄–citric acid buffer, Assay: 160 mM Na₂HPO₄–citric acid buffer

Incubation: 6, Assay: 6

Incubation: 37°C, Assay: 37°C

5 mM p-Coumaric acid

4-Vinylphenol, Carbon Dioxide (CO2)

—

Incubation: 200 rpm

Non-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase

Stability - Incubation

Activity after incubation (12 hour at 25°C), measured by HPLC in aqueous phase

1-Octanol

30%

100.0

98.71

Incubation: 160 mM Na₂HPO₄–citric acid buffer, Assay: 160 mM Na₂HPO₄–citric acid buffer

Incubation: 6, Assay: 6

Incubation: 37°C, Assay: 37°C

5 mM p-Coumaric acid

4-Vinylphenol, Carbon Dioxide (CO2)

—

Incubation: 200 rpm

Non-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase

Stability - Incubation

Activity after incubation (12 hour at 25°C), measured by HPLC in aqueous phase

1-Octanol

50%

100.0

90.86

Incubation: 160 mM Na₂HPO₄–citric acid buffer, Assay: 160 mM Na₂HPO₄–citric acid buffer

Incubation: 6, Assay: 6

Incubation: 37°C, Assay: 37°C

5 mM p-Coumaric acid

4-Vinylphenol, Carbon Dioxide (CO2)

—

Incubation: 200 rpm

Non-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase

Stability - Incubation

Activity after incubation (12 hour at 25°C), measured by HPLC in aqueous phase

Cyclohexane

20%

100.0

102.39

Incubation: 160 mM Na₂HPO₄–citric acid buffer, Assay: 160 mM Na₂HPO₄–citric acid buffer

Incubation: 6, Assay: 6

Incubation: 37°C, Assay: 37°C

5 mM p-Coumaric acid

4-Vinylphenol, Carbon Dioxide (CO2)

—

Incubation: 200 rpm

Non-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase

Stability - Incubation

Activity after incubation (12 hour at 25°C), measured by HPLC in aqueous phase

Cyclohexane

30%

100.0

102.77

Incubation: 160 mM Na₂HPO₄–citric acid buffer, Assay: 160 mM Na₂HPO₄–citric acid buffer

Incubation: 6, Assay: 6

Incubation: 37°C, Assay: 37°C

5 mM p-Coumaric acid

4-Vinylphenol, Carbon Dioxide (CO2)

—

Incubation: 200 rpm

Non-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase

Stability - Incubation

Activity after incubation (12 hour at 25°C), measured by HPLC in aqueous phase

Cyclohexane

50%

100.0

108.37

Incubation: 160 mM Na₂HPO₄–citric acid buffer, Assay: 160 mM Na₂HPO₄–citric acid buffer

Incubation: 6, Assay: 6

Incubation: 37°C, Assay: 37°C

5 mM p-Coumaric acid

4-Vinylphenol, Carbon Dioxide (CO2)

—

Incubation: 200 rpm

Non-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase

Stability - Incubation

Activity after incubation (12 hour at 25°C), measured by HPLC in aqueous phase

n-Hexane

20%

100.0

93.29

Incubation: 160 mM Na₂HPO₄–citric acid buffer, Assay: 160 mM Na₂HPO₄–citric acid buffer

Incubation: 6, Assay: 6

Incubation: 37°C, Assay: 37°C

5 mM p-Coumaric acid

4-Vinylphenol, Carbon Dioxide (CO2)

—

Incubation: 200 rpm

Non-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase

Stability - Incubation

Activity after incubation (12 hour at 25°C), measured by HPLC in aqueous phase

n-Hexane

30%

100.0

102.58

Incubation: 160 mM Na₂HPO₄–citric acid buffer, Assay: 160 mM Na₂HPO₄–citric acid buffer

Incubation: 6, Assay: 6

Incubation: 37°C, Assay: 37°C

5 mM p-Coumaric acid

4-Vinylphenol, Carbon Dioxide (CO2)

—

Incubation: 200 rpm

Non-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase

Stability - Incubation

Activity after incubation (12 hour at 25°C), measured by HPLC in aqueous phase

Toluene

20%

100.0

131.27

Incubation: 160 mM Na₂HPO₄–citric acid buffer, Assay: 160 mM Na₂HPO₄–citric acid buffer

Incubation: 6, Assay: 6

Incubation: 37°C, Assay: 37°C

5 mM p-Coumaric acid

4-Vinylphenol, Carbon Dioxide (CO2)

—

Incubation: 200 rpm

Non-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase

Stability - Incubation

Activity after incubation (12 hour at 25°C), measured by HPLC in aqueous phase

n-Heptane

20%

100.0

99.89

Incubation: 160 mM Na₂HPO₄–citric acid buffer, Assay: 160 mM Na₂HPO₄–citric acid buffer

Incubation: 6, Assay: 6

Incubation: 37°C, Assay: 37°C

5 mM p-Coumaric acid

4-Vinylphenol, Carbon Dioxide (CO2)

—

Incubation: 200 rpm

Non-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase

Stability - Incubation

Activity after incubation (12 hour at 25°C), measured by HPLC in aqueous phase

n-Heptane

30%

100.0

102.23

Incubation: 160 mM Na₂HPO₄–citric acid buffer, Assay: 160 mM Na₂HPO₄–citric acid buffer

Incubation: 6, Assay: 6

Incubation: 37°C, Assay: 37°C

5 mM p-Coumaric acid

4-Vinylphenol, Carbon Dioxide (CO2)

—

Incubation: 200 rpm

Non-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase

Stability - Incubation

Activity after incubation (12 hour at 25°C), measured by HPLC in aqueous phase

n-Heptane

50%

100.0

100.7

Incubation: 160 mM Na₂HPO₄–citric acid buffer, Assay: 160 mM Na₂HPO₄–citric acid buffer

Incubation: 6, Assay: 6

Incubation: 37°C, Assay: 37°C

5 mM p-Coumaric acid

4-Vinylphenol, Carbon Dioxide (CO2)

—

Incubation: 200 rpm

Non-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase

Stability - Incubation

Activity after incubation (12 hour at 25°C), measured by HPLC in aqueous phase

Petroleum Ether

20%

100.0

103.93

Incubation: 160 mM Na₂HPO₄–citric acid buffer, Assay: 160 mM Na₂HPO₄–citric acid buffer

Incubation: 6, Assay: 6

Incubation: 37°C, Assay: 37°C

5 mM p-Coumaric acid

4-Vinylphenol, Carbon Dioxide (CO2)

—

Incubation: 200 rpm

Non-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase

Stability - Incubation

Activity after incubation (12 hour at 25°C), measured by HPLC in aqueous phase

Petroleum Ether

30%

100.0

105.56

Incubation: 160 mM Na₂HPO₄–citric acid buffer, Assay: 160 mM Na₂HPO₄–citric acid buffer

Incubation: 6, Assay: 6

Incubation: 37°C, Assay: 37°C

5 mM p-Coumaric acid

4-Vinylphenol, Carbon Dioxide (CO2)

—

Incubation: 200 rpm

Non-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase

Stability - Incubation

Activity after incubation (12 hour at 25°C), measured by HPLC in aqueous phase

Petroleum Ether

50%

100.0

107.42

Incubation: 160 mM Na₂HPO₄–citric acid buffer, Assay: 160 mM Na₂HPO₄–citric acid buffer

Incubation: 6, Assay: 6

Incubation: 37°C, Assay: 37°C

5 mM p-Coumaric acid

4-Vinylphenol, Carbon Dioxide (CO2)

—

Incubation: 200 rpm

Non-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase

Stability - Incubation

Activity after incubation (12 hour at 25°C), measured by HPLC in aqueous phase

n-Octane

20%

100.0

102.47

Incubation: 160 mM Na₂HPO₄–citric acid buffer, Assay: 160 mM Na₂HPO₄–citric acid buffer

Incubation: 6, Assay: 6

Incubation: 37°C, Assay: 37°C

5 mM p-Coumaric acid

4-Vinylphenol, Carbon Dioxide (CO2)

—

Incubation: 200 rpm

Non-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase

Stability - Incubation

Activity after incubation (12 hour at 25°C), measured by HPLC in aqueous phase

n-Octane

30%

100.0

106.02

Incubation: 160 mM Na₂HPO₄–citric acid buffer, Assay: 160 mM Na₂HPO₄–citric acid buffer

Incubation: 6, Assay: 6

Incubation: 37°C, Assay: 37°C

5 mM p-Coumaric acid

4-Vinylphenol, Carbon Dioxide (CO2)

—

Incubation: 200 rpm

Non-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase

Stability - Incubation

Activity after incubation (12 hour at 25°C), measured by HPLC in aqueous phase

n-Octane

50%

100.0

106.84

Incubation: 160 mM Na₂HPO₄–citric acid buffer, Assay: 160 mM Na₂HPO₄–citric acid buffer

Incubation: 6, Assay: 6

Incubation: 37°C, Assay: 37°C

5 mM p-Coumaric acid

4-Vinylphenol, Carbon Dioxide (CO2)

—

Incubation: 200 rpm

Non-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase

Stability - Incubation

Activity after incubation (12 hour at 25°C), measured by HPLC in aqueous phase

n-Decane

20%

100.0

93.23

Incubation: 160 mM Na₂HPO₄–citric acid buffer, Assay: 160 mM Na₂HPO₄–citric acid buffer

Incubation: 6, Assay: 6

Incubation: 37°C, Assay: 37°C

5 mM p-Coumaric acid

4-Vinylphenol, Carbon Dioxide (CO2)

—

Incubation: 200 rpm

Non-incubated control (in %), assay measured in aqueous phase | Clear about assay in aqueous phase

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Visualization : Activity — Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Visualization : Stability — Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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Phenolic acid decarboxylase (gene blpad) from Bacillus licheniformis

Enzyme Description

Sequence

Experimental Data (45 measurements)

Visualization : Activity — Classical

Visualization : Stability — Incubation

Structure

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