| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
Glycerol |
5% |
100.0 |
109.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
Formamide |
5% |
100.0 |
73.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
Ethylene Glycol |
5% |
100.0 |
95.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
PEG-1000 |
5% |
100.0 |
136.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
PEG-10000 |
5% |
100.0 |
102.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
Dimethylformamide (DMF) |
5% |
100.0 |
121.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
Dimethyl Sulfoxide (DMSO) |
5% |
100.0 |
101.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
Methanol |
5% |
100.0 |
93.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
1,4-Dioxane |
5% |
100.0 |
121.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
Acetonitrile |
5% |
100.0 |
117.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
Ethanol |
5% |
100.0 |
106.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
Acetone |
5% |
100.0 |
113.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
Isopropanol |
5% |
100.0 |
147.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
Tetrahydrofuran (THF) |
5% |
100.0 |
141.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
Glycerol |
10% |
100.0 |
102.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
Formamide |
10% |
100.0 |
76.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
Ethylene Glycol |
10% |
100.0 |
131.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
PEG-1000 |
10% |
100.0 |
136.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
PEG-10000 |
10% |
100.0 |
98.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
Dimethylformamide (DMF) |
10% |
100.0 |
131.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
Dimethyl Sulfoxide (DMSO) |
10% |
100.0 |
107.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
Methanol |
10% |
100.0 |
128.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
1,4-Dioxane |
10% |
100.0 |
139.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
Acetonitrile |
10% |
100.0 |
124.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
Ethanol |
10% |
100.0 |
91.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
Acetone |
10% |
100.0 |
145.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
Isopropanol |
10% |
100.0 |
150.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
Tetrahydrofuran (THF) |
10% |
100.0 |
78.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
Glycerol |
20% |
100.0 |
139.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
Formamide |
20% |
100.0 |
16.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
Ethylene Glycol |
20% |
100.0 |
152.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
PEG-1000 |
20% |
100.0 |
139.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
PEG-10000 |
20% |
100.0 |
53.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
Dimethylformamide (DMF) |
20% |
100.0 |
105.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
Dimethyl Sulfoxide (DMSO) |
20% |
100.0 |
109.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
Methanol |
20% |
100.0 |
146.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
1,4-Dioxane |
20% |
100.0 |
35.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
Acetonitrile |
20% |
100.0 |
10.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
Ethanol |
20% |
100.0 |
102.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
Acetone |
20% |
100.0 |
105.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
Isopropanol |
20% |
100.0 |
101.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
Tetrahydrofuran (THF) |
20% |
100.0 |
0.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
Glycerol |
30% |
100.0 |
145.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
Formamide |
30% |
100.0 |
0.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
Ethylene Glycol |
30% |
100.0 |
184.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
PEG-1000 |
30% |
100.0 |
142.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
PEG-10000 |
30% |
100.0 |
57.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
Dimethylformamide (DMF) |
30% |
100.0 |
8.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
Dimethyl Sulfoxide (DMSO) |
30% |
100.0 |
101.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
Methanol |
30% |
100.0 |
143.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
1,4-Dioxane |
30% |
100.0 |
12.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
Acetonitrile |
30% |
100.0 |
0.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
Ethanol |
30% |
100.0 |
19.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
Acetone |
30% |
100.0 |
14.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
Isopropanol |
30% |
100.0 |
8.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
Tetrahydrofuran (THF) |
30% |
100.0 |
0.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
Glycerol |
40% |
100.0 |
145.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
Formamide |
40% |
100.0 |
0.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
Ethylene Glycol |
40% |
100.0 |
182.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
PEG-1000 |
40% |
100.0 |
139.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
PEG-10000 |
40% |
100.0 |
46.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
Dimethylformamide (DMF) |
40% |
100.0 |
0.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
Dimethyl Sulfoxide (DMSO) |
40% |
100.0 |
72.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
Methanol |
40% |
100.0 |
12.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
1,4-Dioxane |
40% |
100.0 |
0.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
Acetonitrile |
40% |
100.0 |
0.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
Ethanol |
40% |
100.0 |
0.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
Acetone |
40% |
100.0 |
0.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
Isopropanol |
40% |
100.0 |
0.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
Tetrahydrofuran (THF) |
40% |
100.0 |
0.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
Glycerol |
50% |
100.0 |
136.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
Formamide |
50% |
100.0 |
0.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
Ethylene Glycol |
50% |
100.0 |
169.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
PEG-1000 |
50% |
100.0 |
137.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
PEG-10000 |
50% |
100.0 |
0.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
Dimethylformamide (DMF) |
50% |
100.0 |
0.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
Dimethyl Sulfoxide (DMSO) |
50% |
100.0 |
25.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
Methanol |
50% |
100.0 |
0.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
1,4-Dioxane |
50% |
100.0 |
0.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
Acetonitrile |
50% |
100.0 |
0.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
Ethanol |
50% |
100.0 |
0.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
Acetone |
50% |
100.0 |
0.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
Isopropanol |
50% |
100.0 |
0.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
Tetrahydrofuran (THF) |
50% |
100.0 |
0.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
Glycerol |
60% |
100.0 |
114.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
Formamide |
60% |
100.0 |
0.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
Ethylene Glycol |
60% |
100.0 |
167.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
PEG-1000 |
60% |
100.0 |
116.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
PEG-10000 |
60% |
100.0 |
0.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
Dimethylformamide (DMF) |
60% |
100.0 |
0.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
Dimethyl Sulfoxide (DMSO) |
60% |
100.0 |
0.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
Methanol |
60% |
100.0 |
0.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
1,4-Dioxane |
60% |
100.0 |
0.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
Acetonitrile |
60% |
100.0 |
0.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
Ethanol |
60% |
100.0 |
0.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
Acetone |
60% |
100.0 |
0.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
Isopropanol |
60% |
100.0 |
0.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
Tetrahydrofuran (THF) |
60% |
100.0 |
0.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
Glycerol |
75% |
100.0 |
108.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
Formamide |
75% |
100.0 |
0.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
Ethylene Glycol |
75% |
100.0 |
43.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
PEG-1000 |
75% |
100.0 |
111.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
PEG-10000 |
75% |
100.0 |
0.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
Dimethylformamide (DMF) |
75% |
100.0 |
0.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
Dimethyl Sulfoxide (DMSO) |
75% |
100.0 |
0.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
Methanol |
75% |
100.0 |
0.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
1,4-Dioxane |
75% |
100.0 |
0.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
Acetonitrile |
75% |
100.0 |
0.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
Ethanol |
75% |
100.0 |
0.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
Acetone |
75% |
100.0 |
0.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
Isopropanol |
75% |
100.0 |
0.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Activity - Classical |
Activity measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
Tetrahydrofuran (THF) |
75% |
100.0 |
0.0 |
% |
glycine 100 mM |
8.6 |
37 |
? mM 1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Classical aqueous control (in %) |
| Specificity - Enantioselectivity |
E-value (positivie values for the R form) measured gas chromatography in the presence of organic solvent in the presence of organic solvent |
Ethylene Glycol |
50% |
1.0 |
3.0 |
relative units, (kcat/Km)R/(kcat/Km |
Tris-sulfate 50 mM |
8.2 |
20 |
0.5 mM 2-Bromobutane |
2-Butanol, Bromide ion |
β |
β |
Classical aqueous control (in relative units, (kcat/Km)R/(kcat/Km)S) |
| Specificity - Enantioselectivity |
E-value (positivie values for the R form) measured gas chromatography in the presence of organic solvent in the presence of organic solvent |
1,4-Dioxane |
20% |
1.0 |
1.0 |
relative units, (kcat/Km)R/(kcat/Km |
Tris-sulfate 50 mM |
8.2 |
20 |
0.5 mM 2-Bromobutane |
2-Butanol, Bromide ion |
β |
β |
Classical aqueous control (in relative units, (kcat/Km)R/(kcat/Km)S) |
| Specificity - Enantioselectivity |
E-value (positivie values for the R form) measured gas chromatography in the presence of organic solvent in the presence of organic solvent |
Ethylene Glycol |
50% |
132.0 |
>200.0 |
relative units, (kcat/Km)R/(kcat/Km |
Tris-sulfate 50 mM |
8.2 |
20 |
0.5 mM 2-Bromopentane |
2-Pentanol, Bromide ion |
β |
β |
Classical aqueous control (in relative units, (kcat/Km)R/(kcat/Km)S) |
| Specificity - Enantioselectivity |
E-value (positivie values for the R form) measured gas chromatography in the presence of organic solvent in the presence of organic solvent |
Formamide |
5% |
132.0 |
93.0 |
relative units, (kcat/Km)R/(kcat/Km |
Tris-sulfate 50 mM |
8.2 |
20 |
0.5 mM 2-Bromopentane |
2-Pentanol, Bromide ion |
β |
β |
Classical aqueous control (in relative units, (kcat/Km)R/(kcat/Km)S) |
| Specificity - Enantioselectivity |
E-value (positivie values for the R form) measured gas chromatography in the presence of organic solvent in the presence of organic solvent |
Dimethylformamide (DMF) |
10% |
132.0 |
133.0 |
relative units, (kcat/Km)R/(kcat/Km |
Tris-sulfate 50 mM |
8.2 |
20 |
0.5 mM 2-Bromopentane |
2-Pentanol, Bromide ion |
β |
β |
Classical aqueous control (in relative units, (kcat/Km)R/(kcat/Km)S) |
| Specificity - Enantioselectivity |
E-value (positivie values for the R form) measured gas chromatography in the presence of organic solvent in the presence of organic solvent |
Dimethyl Sulfoxide (DMSO) |
20% |
132.0 |
143.0 |
relative units, (kcat/Km)R/(kcat/Km |
Tris-sulfate 50 mM |
8.2 |
20 |
0.5 mM 2-Bromopentane |
2-Pentanol, Bromide ion |
β |
β |
Classical aqueous control (in relative units, (kcat/Km)R/(kcat/Km)S) |
| Specificity - Enantioselectivity |
E-value (positivie values for the R form) measured gas chromatography in the presence of organic solvent in the presence of organic solvent |
Methanol |
20% |
132.0 |
124.0 |
relative units, (kcat/Km)R/(kcat/Km |
Tris-sulfate 50 mM |
8.2 |
20 |
0.5 mM 2-Bromopentane |
2-Pentanol, Bromide ion |
β |
β |
Classical aqueous control (in relative units, (kcat/Km)R/(kcat/Km)S) |
| Specificity - Enantioselectivity |
E-value (positivie values for the R form) measured gas chromatography in the presence of organic solvent in the presence of organic solvent |
1,4-Dioxane |
10% |
132.0 |
194.0 |
relative units, (kcat/Km)R/(kcat/Km |
Tris-sulfate 50 mM |
8.2 |
20 |
0.5 mM 2-Bromopentane |
2-Pentanol, Bromide ion |
β |
β |
Classical aqueous control (in relative units, (kcat/Km)R/(kcat/Km)S) |
| Specificity - Enantioselectivity |
E-value (positivie values for the R form) measured gas chromatography in the presence of organic solvent in the presence of organic solvent |
1,4-Dioxane |
20% |
132.0 |
>200.0 |
relative units, (kcat/Km)R/(kcat/Km |
Tris-sulfate 50 mM |
8.2 |
20 |
0.5 mM 2-Bromopentane |
2-Pentanol, Bromide ion |
β |
β |
Classical aqueous control (in relative units, (kcat/Km)R/(kcat/Km)S) |
| Specificity - Enantioselectivity |
E-value (positivie values for the R form) measured gas chromatography in the presence of organic solvent in the presence of organic solvent |
Acetonitrile |
10% |
132.0 |
134.0 |
relative units, (kcat/Km)R/(kcat/Km |
Tris-sulfate 50 mM |
8.2 |
20 |
0.5 mM 2-Bromopentane |
2-Pentanol, Bromide ion |
β |
β |
Classical aqueous control (in relative units, (kcat/Km)R/(kcat/Km)S) |
| Specificity - Enantioselectivity |
E-value (positivie values for the R form) measured gas chromatography in the presence of organic solvent in the presence of organic solvent |
Ethanol |
20% |
132.0 |
29.0 |
relative units, (kcat/Km)R/(kcat/Km |
Tris-sulfate 50 mM |
8.2 |
20 |
0.5 mM 2-Bromopentane |
2-Pentanol, Bromide ion |
β |
β |
Classical aqueous control (in relative units, (kcat/Km)R/(kcat/Km)S) |
| Specificity - Enantioselectivity |
E-value (positivie values for the R form) measured gas chromatography in the presence of organic solvent in the presence of organic solvent |
Acetone |
10% |
132.0 |
107.0 |
relative units, (kcat/Km)R/(kcat/Km |
Tris-sulfate 50 mM |
8.2 |
20 |
0.5 mM 2-Bromopentane |
2-Pentanol, Bromide ion |
β |
β |
Classical aqueous control (in relative units, (kcat/Km)R/(kcat/Km)S) |
| Specificity - Enantioselectivity |
E-value (positivie values for the R form) measured gas chromatography in the presence of organic solvent in the presence of organic solvent |
Isopropanol |
10% |
132.0 |
27.0 |
relative units, (kcat/Km)R/(kcat/Km |
Tris-sulfate 50 mM |
8.2 |
20 |
0.5 mM 2-Bromopentane |
2-Pentanol, Bromide ion |
β |
β |
Classical aqueous control (in relative units, (kcat/Km)R/(kcat/Km)S) |
| Specificity - Enantioselectivity |
E-value (positivie values for the R form) measured gas chromatography in the presence of organic solvent in the presence of organic solvent |
Tetrahydrofuran (THF) |
5% |
132.0 |
29.0 |
relative units, (kcat/Km)R/(kcat/Km |
Tris-sulfate 50 mM |
8.2 |
20 |
0.5 mM 2-Bromopentane |
2-Pentanol, Bromide ion |
β |
β |
Classical aqueous control (in relative units, (kcat/Km)R/(kcat/Km)S) |
| Specificity - Enantioselectivity |
E-value (positivie values for the R form) measured gas chromatography in the presence of organic solvent in the presence of organic solvent |
Ethylene Glycol |
50% |
8.0 |
30.0 |
relative units, (kcat/Km)R/(kcat/Km |
Tris-sulfate 50 mM |
8.2 |
20 |
0.5 mM 2-Bromohexane |
2-Hexanol, Bromide ion |
β |
β |
Classical aqueous control (in relative units, (kcat/Km)R/(kcat/Km)S) |
| Specificity - Enantioselectivity |
E-value (positivie values for the R form) measured gas chromatography in the presence of organic solvent in the presence of organic solvent |
1,4-Dioxane |
20% |
8.0 |
48.0 |
relative units, (kcat/Km)R/(kcat/Km |
Tris-sulfate 50 mM |
8.2 |
20 |
0.5 mM 2-Bromohexane |
2-Hexanol, Bromide ion |
β |
β |
Classical aqueous control (in relative units, (kcat/Km)R/(kcat/Km)S) |
| Stability - C50 |
Volume fraction of organic solvent at which 50% of enzyme activity is lost, measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
Glycerol |
β |
β |
75.0 |
% (v/v) |
glycine 100 mM |
8.6 |
37 |
1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Not applicable control (in % (v/v)) |
| Stability - C50 |
Volume fraction of organic solvent at which 50% of enzyme activity is lost, measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
Formamide |
β |
β |
14.0 |
% (v/v) |
glycine 100 mM |
8.6 |
37 |
1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Not applicable control (in % (v/v)) |
| Stability - C50 |
Volume fraction of organic solvent at which 50% of enzyme activity is lost, measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
Ethylene Glycol |
β |
β |
74.0 |
% (v/v) |
glycine 100 mM |
8.6 |
37 |
1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Not applicable control (in % (v/v)) |
| Stability - C50 |
Volume fraction of organic solvent at which 50% of enzyme activity is lost, measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
Dimethyl Sulfoxide (DMSO) |
β |
β |
44.0 |
% (v/v) |
glycine 100 mM |
8.6 |
37 |
1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Not applicable control (in % (v/v)) |
| Stability - C50 |
Volume fraction of organic solvent at which 50% of enzyme activity is lost, measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
Dimethylformamide (DMF) |
β |
β |
26.0 |
% (v/v) |
glycine 100 mM |
8.6 |
37 |
1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Not applicable control (in % (v/v)) |
| Stability - C50 |
Volume fraction of organic solvent at which 50% of enzyme activity is lost, measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
Methanol |
β |
β |
37.0 |
% (v/v) |
glycine 100 mM |
8.6 |
37 |
1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Not applicable control (in % (v/v)) |
| Stability - C50 |
Volume fraction of organic solvent at which 50% of enzyme activity is lost, measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
Acetonitrile |
β |
β |
15.0 |
% (v/v) |
glycine 100 mM |
8.6 |
37 |
1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Not applicable control (in % (v/v)) |
| Stability - C50 |
Volume fraction of organic solvent at which 50% of enzyme activity is lost, measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
Ethanol |
β |
β |
21.0 |
% (v/v) |
glycine 100 mM |
8.6 |
37 |
1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Not applicable control (in % (v/v)) |
| Stability - C50 |
Volume fraction of organic solvent at which 50% of enzyme activity is lost, measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
1,4-Dioxane |
β |
β |
20.0 |
% (v/v) |
glycine 100 mM |
8.6 |
37 |
1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Not applicable control (in % (v/v)) |
| Stability - C50 |
Volume fraction of organic solvent at which 50% of enzyme activity is lost, measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
Acetone |
β |
β |
26.0 |
% (v/v) |
glycine 100 mM |
8.6 |
37 |
1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Not applicable control (in % (v/v)) |
| Stability - C50 |
Volume fraction of organic solvent at which 50% of enzyme activity is lost, measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
Isopropanol |
β |
β |
25.0 |
% (v/v) |
glycine 100 mM |
8.6 |
37 |
1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Not applicable control (in % (v/v)) |
| Stability - C50 |
Volume fraction of organic solvent at which 50% of enzyme activity is lost, measured by absorbance spectrophotometry (colorimetric assay, iodine, mercuric thiocyanate and ferric ammonium sulfate reaction product FeβSCN absorbance measurement, 460 nm) in the presence of organic solvent |
Tetrahydrofuran (THF) |
β |
β |
12.0 |
% (v/v) |
glycine 100 mM |
8.6 |
37 |
1-Iodohexane |
1-Hexanol, Iodide ion |
β |
β |
Not applicable control (in % (v/v)) |
| Stability - Tm |
Tm measured by circular dichroism in the presence of organic solvent |
Ethylene Glycol |
20% |
52.5 |
51.0 |
Β°C |
50 mM phosphate buffer |
7.5 |
β |
β |
β |
β |
β |
Classical aqueous control (in Β°C), averaged between circular dichroism and DSC measurements |
| Stability - Tm |
Tm measured by DSC in the presence of organic solvent |
Dimethylformamide (DMF) |
20% |
52.5 |
36.0 |
Β°C |
50 mM phosphate buffer |
7.5 |
β |
β |
β |
β |
β |
Classical aqueous control (in Β°C), averaged between circular dichroism and DSC measurements |
| Stability - Tm |
Tm measured by DSC in the presence of organic solvent |
Dimethyl Sulfoxide (DMSO) |
20% |
52.5 |
48.0 |
Β°C |
50 mM phosphate buffer |
7.5 |
β |
β |
β |
β |
β |
Classical aqueous control (in Β°C), averaged between circular dichroism and DSC measurements |
| Stability - Tm |
Tm measured by circular dichroism in the presence of organic solvent |
Methanol |
20% |
52.5 |
46.0 |
Β°C |
50 mM phosphate buffer |
7.5 |
β |
β |
β |
β |
β |
Classical aqueous control (in Β°C), averaged between circular dichroism and DSC measurements |
| Stability - Tm |
Tm measured by circular dichroism in the presence of organic solvent |
1,4-Dioxane |
20% |
52.5 |
35.0 |
Β°C |
50 mM phosphate buffer |
7.5 |
β |
β |
β |
β |
β |
Classical aqueous control (in Β°C), averaged between circular dichroism and DSC measurements |
| Stability - Tm |
Tm measured by circular dichroism in the presence of organic solvent |
Acetonitrile |
10% |
52.5 |
41.0 |
Β°C |
50 mM phosphate buffer |
7.5 |
β |
β |
β |
β |
β |
Classical aqueous control (in Β°C), averaged between circular dichroism and DSC measurements |
| Stability - Tm |
Tm measured by circular dichroism in the presence of organic solvent |
Acetonitrile |
20% |
52.5 |
0.0 |
Β°C |
50 mM phosphate buffer |
7.5 |
β |
β |
β |
β |
β |
Classical aqueous control (in Β°C), averaged between circular dichroism and DSC measurements |
| Stability - Tm |
Tm measured by circular dichroism in the presence of organic solvent |
Ethanol |
20% |
52.5 |
40.0 |
Β°C |
50 mM phosphate buffer |
7.5 |
β |
β |
β |
β |
β |
Classical aqueous control (in Β°C), averaged between circular dichroism and DSC measurements |
| Stability - Tm |
Tm measured by circular dichroism in the presence of organic solvent |
Acetone |
20% |
52.5 |
34.0 |
Β°C |
50 mM phosphate buffer |
7.5 |
β |
β |
β |
β |
β |
Classical aqueous control (in Β°C), averaged between circular dichroism and DSC measurements |
| Stability - Tm |
Tm measured by circular dichroism in the presence of organic solvent |
Isopropanol |
20% |
52.5 |
36.0 |
Β°C |
50 mM phosphate buffer |
7.5 |
β |
β |
β |
β |
β |
Classical aqueous control (in Β°C), averaged between circular dichroism and DSC measurements |
| Stability - Tm |
Tm measured by circular dichroism in the presence of organic solvent |
Tetrahydrofuran (THF) |
10% |
52.5 |
34.0 |
Β°C |
50 mM phosphate buffer |
7.5 |
β |
β |
β |
β |
β |
Classical aqueous control (in Β°C), averaged between circular dichroism and DSC measurements |
| Stability - Tm |
Tm measured by circular dichroism in the presence of organic solvent |
Tetrahydrofuran (THF) |
20% |
52.5 |
<10.0 |
Β°C |
50 mM phosphate buffer |
7.5 |
β |
β |
β |
β |
β |
Classical aqueous control (in Β°C), averaged between circular dichroism and DSC measurements |