Formate oxidase (gene AnFOX) from Aspergillus nomiae
Enzyme Description
Sequence
MSAQVNNNQYDFIVVGGGTAGNAIAGRLSENPSVRVLVVEAGIANSGQIEDITTPSKAFGLRGSQYDWAYKTTMIKRDDYERVEKPNTRGKALGGSSCANYFTWIPGSKATFDDWYEFGGSDWTWDNCVDYLRKCATYHDDEGLYPTDLNKIGTGGPVQISHAELVPELETFRNALTTAWTSKGEPLSEDIYSGEMHGLTHCVDTIYKGQRQGSWLYLENKPNVTILSQVHSKRLIIDRTTQTCTGVTVVDPTNGAELNLYATREVIVSQGVFETPKLLLLSGIGPAAELNKHGIDVVVDSPHVGKNLLDHPIVPFVLRLKDGVALDDHIHRAGPANEAAVAAYRRDKTGPAASGFLELVGFPRIDERLNRYPAYREAKAANGGLDPFGPAGQPHFELDFVGLFSSAFQWHYPVPPSGSYMTVIVDLLRPVSEGGEVTLNSTDALQQPNINLNFFASDLDVLAMREGVRWTYDVLTKGEGFKDLVLEEYPWNMPLDSDEAMNKAVLDRSQTGFHPCGTARLGKNVQQGVVDSKLRVHGIKNLRVADASIMPVIPDCRIQNSVYMIGEKGADIIKAQYRELYEQKNIPYFSSKL
Zhenyu Zhai et al. (2025)
β Discovery and characterization of high-activity formate oxidase with low Km value under nonacidic conditions
Bioscience, Biotechnology, and Biochemistry
Β· doi:10.1093/bbb/zbaf092 β
Β· Activity - Classical
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Database ID
UniProt: A0A0L1IR91 β
Sequence Annotation
Explicit - Provided UniProt Accesion Number
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)
Experimental Data (36 measurements)
36 measurements β page 1 of 2
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Activity - Classical | Activity measured by absorbance spectrophotometry (horseradish peroxidase-catalyzed H2O2 product reaction with ABTS absorbance measurement, 405 nm) in the presence of organic solvent | Glycerol | 20% | 100.0 | 223.5 | % | 100 mM sodium phosphate buffer | ? | 25Β°C | Formate, Molecular oxygen | Carbon Dioxide (CO2) , HβOβ (Hydrogen Peroxide) | FAD | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (horseradish peroxidase-catalyzed H2O2 product reaction with ABTS absorbance measurement, 405 nm) in the presence of organic solvent | Dichloromethane | 10% | 100.0 | 87.2 | % | 100 mM sodium phosphate buffer | ? | 25Β°C | Formate, Molecular oxygen | Carbon Dioxide (CO2) , HβOβ (Hydrogen Peroxide) | FAD | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (horseradish peroxidase-catalyzed H2O2 product reaction with ABTS absorbance measurement, 405 nm) in the presence of organic solvent | Methanol | 10% | 100.0 | 86.2 | % | 100 mM sodium phosphate buffer | ? | 25Β°C | Formate, Molecular oxygen | Carbon Dioxide (CO2) , HβOβ (Hydrogen Peroxide) | FAD | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (horseradish peroxidase-catalyzed H2O2 product reaction with ABTS absorbance measurement, 405 nm) in the presence of organic solvent | Ethanol | 10% | 100.0 | 95.9 | % | 100 mM sodium phosphate buffer | ? | 25Β°C | Formate, Molecular oxygen | Carbon Dioxide (CO2) , HβOβ (Hydrogen Peroxide) | FAD | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (horseradish peroxidase-catalyzed H2O2 product reaction with ABTS absorbance measurement, 405 nm) in the presence of organic solvent | Isobutanol | 10% | 100.0 | 92.8 | % | 100 mM sodium phosphate buffer | ? | 25Β°C | Formate, Molecular oxygen | Carbon Dioxide (CO2) , HβOβ (Hydrogen Peroxide) | FAD | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (horseradish peroxidase-catalyzed H2O2 product reaction with ABTS absorbance measurement, 405 nm) in the presence of organic solvent | 1-Propanol | 10% | 100.0 | 28.1 | % | 100 mM sodium phosphate buffer | ? | 25Β°C | Formate, Molecular oxygen | Carbon Dioxide (CO2) , HβOβ (Hydrogen Peroxide) | FAD | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (horseradish peroxidase-catalyzed H2O2 product reaction with ABTS absorbance measurement, 405 nm) in the presence of organic solvent | Acetonitrile | 10% | 100.0 | 33.7 | % | 100 mM sodium phosphate buffer | ? | 25Β°C | Formate, Molecular oxygen | Carbon Dioxide (CO2) , HβOβ (Hydrogen Peroxide) | FAD | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (horseradish peroxidase-catalyzed H2O2 product reaction with ABTS absorbance measurement, 405 nm) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 10% | 100.0 | 174.5 | % | 100 mM sodium phosphate buffer | ? | 25Β°C | Formate, Molecular oxygen | Carbon Dioxide (CO2) , HβOβ (Hydrogen Peroxide) | FAD | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (horseradish peroxidase-catalyzed H2O2 product reaction with ABTS absorbance measurement, 405 nm) in the presence of organic solvent | Dimethylformamide (DMF) | 10% | 100.0 | 127.0 | % | 100 mM sodium phosphate buffer | ? | 25Β°C | Formate, Molecular oxygen | Carbon Dioxide (CO2) , HβOβ (Hydrogen Peroxide) | FAD | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (horseradish peroxidase-catalyzed H2O2 product reaction with ABTS absorbance measurement, 405 nm) in the presence of organic solvent | Glycerol | 10% | 100.0 | 135.7 | % | 100 mM sodium phosphate buffer | ? | 25Β°C | Formate, Molecular oxygen | Carbon Dioxide (CO2) , HβOβ (Hydrogen Peroxide) | FAD | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (horseradish peroxidase-catalyzed H2O2 product reaction with ABTS absorbance measurement, 405 nm) in the presence of organic solvent | Dichloromethane | 20% | 100.0 | 67.3 | % | 100 mM sodium phosphate buffer | ? | 25Β°C | Formate, Molecular oxygen | Carbon Dioxide (CO2) , HβOβ (Hydrogen Peroxide) | FAD | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (horseradish peroxidase-catalyzed H2O2 product reaction with ABTS absorbance measurement, 405 nm) in the presence of organic solvent | Methanol | 20% | 100.0 | 53.1 | % | 100 mM sodium phosphate buffer | ? | 25Β°C | Formate, Molecular oxygen | Carbon Dioxide (CO2) , HβOβ (Hydrogen Peroxide) | FAD | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (horseradish peroxidase-catalyzed H2O2 product reaction with ABTS absorbance measurement, 405 nm) in the presence of organic solvent | Ethanol | 20% | 100.0 | 49.5 | % | 100 mM sodium phosphate buffer | ? | 25Β°C | Formate, Molecular oxygen | Carbon Dioxide (CO2) , HβOβ (Hydrogen Peroxide) | FAD | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (horseradish peroxidase-catalyzed H2O2 product reaction with ABTS absorbance measurement, 405 nm) in the presence of organic solvent | Isobutanol | 20% | 100.0 | 99.5 | % | 100 mM sodium phosphate buffer | ? | 25Β°C | Formate, Molecular oxygen | Carbon Dioxide (CO2) , HβOβ (Hydrogen Peroxide) | FAD | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (horseradish peroxidase-catalyzed H2O2 product reaction with ABTS absorbance measurement, 405 nm) in the presence of organic solvent | 1-Propanol | 20% | 100.0 | 0.0 | % | 100 mM sodium phosphate buffer | ? | 25Β°C | Formate, Molecular oxygen | Carbon Dioxide (CO2) , HβOβ (Hydrogen Peroxide) | FAD | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (horseradish peroxidase-catalyzed H2O2 product reaction with ABTS absorbance measurement, 405 nm) in the presence of organic solvent | Acetonitrile | 20% | 100.0 | 0.0 | % | 100 mM sodium phosphate buffer | ? | 25Β°C | Formate, Molecular oxygen | Carbon Dioxide (CO2) , HβOβ (Hydrogen Peroxide) | FAD | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (horseradish peroxidase-catalyzed H2O2 product reaction with ABTS absorbance measurement, 405 nm) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 20% | 100.0 | 142.3 | % | 100 mM sodium phosphate buffer | ? | 25Β°C | Formate, Molecular oxygen | Carbon Dioxide (CO2) , HβOβ (Hydrogen Peroxide) | FAD | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (horseradish peroxidase-catalyzed H2O2 product reaction with ABTS absorbance measurement, 405 nm) in the presence of organic solvent | Dimethylformamide (DMF) | 20% | 100.0 | 44.4 | % | 100 mM sodium phosphate buffer | ? | 25Β°C | Formate, Molecular oxygen | Carbon Dioxide (CO2) , HβOβ (Hydrogen Peroxide) | FAD | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (horseradish peroxidase-catalyzed H2O2 product reaction with ABTS absorbance measurement, 405 nm) in the presence of organic solvent | Glycerol | 5% | 100.0 | 102.5 | % | 100 mM sodium phosphate buffer | ? | 25Β°C | Formate, Molecular oxygen | Carbon Dioxide (CO2) , HβOβ (Hydrogen Peroxide) | FAD | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (horseradish peroxidase-catalyzed H2O2 product reaction with ABTS absorbance measurement, 405 nm) in the presence of organic solvent | Dichloromethane | 2% | 100.0 | 81.1 | % | 100 mM sodium phosphate buffer | ? | 25Β°C | Formate, Molecular oxygen | Carbon Dioxide (CO2) , HβOβ (Hydrogen Peroxide) | FAD | β | Classical aqueous control (in %) |
Visualization : Activity β Classical
One bar per measurement. Colour = solvent, shade = solvent volume.