Formate oxidase (gene AnFOX) from Aspergillus nomiae

Enzyme Description

Species
Aspergillus nomiae β†—
Extremophile
No
EC Number
1.17.1.9 β†—

Sequence

Length: 593 amino acids
MSAQVNNNQYDFIVVGGGTAGNAIAGRLSENPSVRVLVVEAGIANSGQIEDITTPSKAFGLRGSQYDWAYKTTMIKRDDYERVEKPNTRGKALGGSSCANYFTWIPGSKATFDDWYEFGGSDWTWDNCVDYLRKCATYHDDEGLYPTDLNKIGTGGPVQISHAELVPELETFRNALTTAWTSKGEPLSEDIYSGEMHGLTHCVDTIYKGQRQGSWLYLENKPNVTILSQVHSKRLIIDRTTQTCTGVTVVDPTNGAELNLYATREVIVSQGVFETPKLLLLSGIGPAAELNKHGIDVVVDSPHVGKNLLDHPIVPFVLRLKDGVALDDHIHRAGPANEAAVAAYRRDKTGPAASGFLELVGFPRIDERLNRYPAYREAKAANGGLDPFGPAGQPHFELDFVGLFSSAFQWHYPVPPSGSYMTVIVDLLRPVSEGGEVTLNSTDALQQPNINLNFFASDLDVLAMREGVRWTYDVLTKGEGFKDLVLEEYPWNMPLDSDEAMNKAVLDRSQTGFHPCGTARLGKNVQQGVVDSKLRVHGIKNLRVADASIMPVIPDCRIQNSVYMIGEKGADIIKAQYRELYEQKNIPYFSSKL
Zhenyu Zhai et al. (2025) β€” Discovery and characterization of high-activity formate oxidase with low Km value under nonacidic conditions
Bioscience, Biotechnology, and Biochemistry  Β· doi:10.1093/bbb/zbaf092 β†—  Β· Activity - Classical
36 measurements ▼
Database ID
UniProt: A0A0L1IR91 β†—
Sequence Annotation
Explicit - Provided UniProt Accesion Number
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (36 measurements)

36 measurements β€” page 2 of 2
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity - Classical Activity measured by absorbance spectrophotometry (horseradish peroxidase-catalyzed H2O2 product reaction with ABTS absorbance measurement, 405 nm) in the presence of organic solvent Methanol 2% 100.0 75.0 % 100 mM sodium phosphate buffer ? 25Β°C Formate, Molecular oxygen Carbon Dioxide (CO2) , Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) FAD β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (horseradish peroxidase-catalyzed H2O2 product reaction with ABTS absorbance measurement, 405 nm) in the presence of organic solvent Ethanol 2% 100.0 80.6 % 100 mM sodium phosphate buffer ? 25Β°C Formate, Molecular oxygen Carbon Dioxide (CO2) , Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) FAD β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (horseradish peroxidase-catalyzed H2O2 product reaction with ABTS absorbance measurement, 405 nm) in the presence of organic solvent Isobutanol 2% 100.0 90.3 % 100 mM sodium phosphate buffer ? 25Β°C Formate, Molecular oxygen Carbon Dioxide (CO2) , Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) FAD β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (horseradish peroxidase-catalyzed H2O2 product reaction with ABTS absorbance measurement, 405 nm) in the presence of organic solvent 1-Propanol 2% 100.0 53.6 % 100 mM sodium phosphate buffer ? 25Β°C Formate, Molecular oxygen Carbon Dioxide (CO2) , Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) FAD β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (horseradish peroxidase-catalyzed H2O2 product reaction with ABTS absorbance measurement, 405 nm) in the presence of organic solvent Acetonitrile 2% 100.0 80.6 % 100 mM sodium phosphate buffer ? 25Β°C Formate, Molecular oxygen Carbon Dioxide (CO2) , Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) FAD β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (horseradish peroxidase-catalyzed H2O2 product reaction with ABTS absorbance measurement, 405 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 2% 100.0 117.3 % 100 mM sodium phosphate buffer ? 25Β°C Formate, Molecular oxygen Carbon Dioxide (CO2) , Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) FAD β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (horseradish peroxidase-catalyzed H2O2 product reaction with ABTS absorbance measurement, 405 nm) in the presence of organic solvent Dimethylformamide (DMF) 2% 100.0 89.8 % 100 mM sodium phosphate buffer ? 25Β°C Formate, Molecular oxygen Carbon Dioxide (CO2) , Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) FAD β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (horseradish peroxidase-catalyzed H2O2 product reaction with ABTS absorbance measurement, 405 nm) in the presence of organic solvent Glycerol 2% 100.0 94.4 % 100 mM sodium phosphate buffer ? 25Β°C Formate, Molecular oxygen Carbon Dioxide (CO2) , Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) FAD β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (horseradish peroxidase-catalyzed H2O2 product reaction with ABTS absorbance measurement, 405 nm) in the presence of organic solvent Dichloromethane 5% 100.0 103.1 % 100 mM sodium phosphate buffer ? 25Β°C Formate, Molecular oxygen Carbon Dioxide (CO2) , Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) FAD β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (horseradish peroxidase-catalyzed H2O2 product reaction with ABTS absorbance measurement, 405 nm) in the presence of organic solvent Methanol 5% 100.0 101.5 % 100 mM sodium phosphate buffer ? 25Β°C Formate, Molecular oxygen Carbon Dioxide (CO2) , Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) FAD β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (horseradish peroxidase-catalyzed H2O2 product reaction with ABTS absorbance measurement, 405 nm) in the presence of organic solvent Ethanol 5% 100.0 100.0 % 100 mM sodium phosphate buffer ? 25Β°C Formate, Molecular oxygen Carbon Dioxide (CO2) , Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) FAD β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (horseradish peroxidase-catalyzed H2O2 product reaction with ABTS absorbance measurement, 405 nm) in the presence of organic solvent Isobutanol 5% 100.0 110.2 % 100 mM sodium phosphate buffer ? 25Β°C Formate, Molecular oxygen Carbon Dioxide (CO2) , Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) FAD β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (horseradish peroxidase-catalyzed H2O2 product reaction with ABTS absorbance measurement, 405 nm) in the presence of organic solvent 1-Propanol 5% 100.0 51.0 % 100 mM sodium phosphate buffer ? 25Β°C Formate, Molecular oxygen Carbon Dioxide (CO2) , Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) FAD β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (horseradish peroxidase-catalyzed H2O2 product reaction with ABTS absorbance measurement, 405 nm) in the presence of organic solvent Acetonitrile 5% 100.0 60.2 % 100 mM sodium phosphate buffer ? 25Β°C Formate, Molecular oxygen Carbon Dioxide (CO2) , Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) FAD β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (horseradish peroxidase-catalyzed H2O2 product reaction with ABTS absorbance measurement, 405 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 5% 100.0 143.9 % 100 mM sodium phosphate buffer ? 25Β°C Formate, Molecular oxygen Carbon Dioxide (CO2) , Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) FAD β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (horseradish peroxidase-catalyzed H2O2 product reaction with ABTS absorbance measurement, 405 nm) in the presence of organic solvent Dimethylformamide (DMF) 5% 100.0 107.1 % 100 mM sodium phosphate buffer ? 25Β°C Formate, Molecular oxygen Carbon Dioxide (CO2) , Hβ‚‚Oβ‚‚ (Hydrogen Peroxide) FAD β€” Classical aqueous control (in %)
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Visualization : Activity β€” Classical

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Structure

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