Asclepain cI from Asclepias curassavica

Enzyme Description

Species
Asclepias curassavica β†—
Extremophile
No
EC Number
3.4.22.7 β†—

Sequence

Length: 194 amino acids
QGKCGSCWTFSAVASIETLIGIKEGRMIALSEQELLDCERTSFGCKGGYYANAFAYVAKNGITSRDRYPYIFQQGQCYQKEKVVKISGYRNVRRNDEKELQLVVAQQVVSIGIKSSSRDFQHYRQGIFNGACGPKLDHAVNIVGYGSEGGANYWIVRNSWGTGWGEGGYARLPMYSGQVGGYCGIVSQASYPVY
Anabella L. Origone et al. (2024) β€” Effect of Organic Solvents on the Activity, Stability and Secondary Structure of asclepain cI, Using FTIR and Molecular Dynamics Simulations
 Β· doi:10.1007/s10930-024-10182-4 β†—  Β· Activity + Stability - Incubation
51 measurements ▼
Database ID
UniProt: B7VF64 β†—
Sequence Annotation
Explicit - Provided UniProt Accesion Number
Protein Source
Purified, eukaryore Asclepias curassavica

Experimental Data (51 measurements)

51 measurements β€” page 1 of 3
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity + Stability - Incubation Catalytic potential after incubation (8 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) with organic solvent Tetrahydrofuran (THF) 70% 197 208 IU/g.h Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer, 0.01 mM L-Cysteine Incubation: 8, Assay: 8 Incubation: 40Β°C, Assay: 40Β°C 0.05 mM Benzoyl-DL-arginine p-nitroanilide N-Ξ±-benzoyl-DL-arginine , p-Nitroaniline β€” Incubation: 220 rpm, Assay: 220 rpm Water-incubated control (in IU/g.h), assay in the presence of organic solvent
Activity + Stability - Incubation Catalytic potential after incubation (8 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) with organic solvent 1-Octanol 50% 197 211 IU/g.h Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer, 0.01 mM L-Cysteine Incubation: 8, Assay: 8 Incubation: 40Β°C, Assay: 40Β°C 0.05 mM Benzoyl-DL-arginine p-nitroanilide N-Ξ±-benzoyl-DL-arginine , p-Nitroaniline β€” Incubation: 220 rpm, Assay: 220 rpm Water-incubated control (in IU/g.h), assay in the presence of organic solvent
Activity + Stability - Incubation Catalytic potential after incubation (8 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) with organic solvent Ethyl Acetate 50% 197 289 IU/g.h Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer, 0.01 mM L-Cysteine Incubation: 8, Assay: 8 Incubation: 40Β°C, Assay: 40Β°C 0.05 mM Benzoyl-DL-arginine p-nitroanilide N-Ξ±-benzoyl-DL-arginine , p-Nitroaniline β€” Incubation: 220 rpm, Assay: 220 rpm Water-incubated control (in IU/g.h), assay in the presence of organic solvent
Activity + Stability - Incubation Catalytic potential after incubation (8 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) with organic solvent Dichloromethane 50% 197 179 IU/g.h Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer, 0.01 mM L-Cysteine Incubation: 8, Assay: 8 Incubation: 40Β°C, Assay: 40Β°C 0.05 mM Benzoyl-DL-arginine p-nitroanilide N-Ξ±-benzoyl-DL-arginine , p-Nitroaniline β€” Incubation: 220 rpm, Assay: 220 rpm Water-incubated control (in IU/g.h), assay in the presence of organic solvent
Activity + Stability - Incubation Catalytic potential after incubation (8 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) with organic solvent Diethyl Ether 50% 197 208 IU/g.h Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer, 0.01 mM L-Cysteine Incubation: 8, Assay: 8 Incubation: 40Β°C, Assay: 40Β°C 0.05 mM Benzoyl-DL-arginine p-nitroanilide N-Ξ±-benzoyl-DL-arginine , p-Nitroaniline β€” Incubation: 220 rpm, Assay: 220 rpm Water-incubated control (in IU/g.h), assay in the presence of organic solvent
Activity + Stability - Incubation Catalytic potential after incubation (8 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) with organic solvent n-Hexane 50% 197 281 IU/g.h Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer, 0.01 mM L-Cysteine Incubation: 8, Assay: 8 Incubation: 40Β°C, Assay: 40Β°C 0.05 mM Benzoyl-DL-arginine p-nitroanilide N-Ξ±-benzoyl-DL-arginine , p-Nitroaniline β€” Incubation: 220 rpm, Assay: 220 rpm Water-incubated control (in IU/g.h), assay in the presence of organic solvent
Activity + Stability - Incubation Catalytic potential after incubation (8 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) with organic solvent Chlorobenzene 50% 197 210 IU/g.h Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer, 0.01 mM L-Cysteine Incubation: 8, Assay: 8 Incubation: 40Β°C, Assay: 40Β°C 0.05 mM Benzoyl-DL-arginine p-nitroanilide N-Ξ±-benzoyl-DL-arginine , p-Nitroaniline β€” Incubation: 220 rpm, Assay: 220 rpm Water-incubated control (in IU/g.h), assay in the presence of organic solvent
Activity + Stability - Incubation Catalytic potential after incubation (8 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) with organic solvent Benzene 50% 197 203 IU/g.h Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer, 0.01 mM L-Cysteine Incubation: 8, Assay: 8 Incubation: 40Β°C, Assay: 40Β°C 0.05 mM Benzoyl-DL-arginine p-nitroanilide N-Ξ±-benzoyl-DL-arginine , p-Nitroaniline β€” Incubation: 220 rpm, Assay: 220 rpm Water-incubated control (in IU/g.h), assay in the presence of organic solvent
Activity + Stability - Incubation Catalytic potential after incubation (8 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) with organic solvent n-Hexadecane 50% 197 0 IU/g.h Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer, 0.01 mM L-Cysteine Incubation: 8, Assay: 8 Incubation: 40Β°C, Assay: 40Β°C 0.05 mM Benzoyl-DL-arginine p-nitroanilide N-Ξ±-benzoyl-DL-arginine , p-Nitroaniline β€” Incubation: 220 rpm, Assay: 220 rpm Water-incubated control (in IU/g.h), assay in the presence of organic solvent
Activity + Stability - Incubation Catalytic potential after incubation (8 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) with organic solvent Ethanol 70% 197 135 IU/g.h Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer, 0.01 mM L-Cysteine Incubation: 8, Assay: 8 Incubation: 40Β°C, Assay: 40Β°C 0.05 mM Benzoyl-DL-arginine p-nitroanilide N-Ξ±-benzoyl-DL-arginine , p-Nitroaniline β€” Incubation: 220 rpm, Assay: 220 rpm Water-incubated control (in IU/g.h), assay in the presence of organic solvent
Activity + Stability - Incubation Catalytic potential after incubation (8 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) with organic solvent Acetonitrile 70% 197 119 IU/g.h Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer, 0.01 mM L-Cysteine Incubation: 8, Assay: 8 Incubation: 40Β°C, Assay: 40Β°C 0.05 mM Benzoyl-DL-arginine p-nitroanilide N-Ξ±-benzoyl-DL-arginine , p-Nitroaniline β€” Incubation: 220 rpm, Assay: 220 rpm Water-incubated control (in IU/g.h), assay in the presence of organic solvent
Activity + Stability - Incubation Catalytic potential after incubation (8 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) with organic solvent Methanol 70% 197 117 IU/g.h Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer, 0.01 mM L-Cysteine Incubation: 8, Assay: 8 Incubation: 40Β°C, Assay: 40Β°C 0.05 mM Benzoyl-DL-arginine p-nitroanilide N-Ξ±-benzoyl-DL-arginine , p-Nitroaniline β€” Incubation: 220 rpm, Assay: 220 rpm Water-incubated control (in IU/g.h), assay in the presence of organic solvent
Activity + Stability - Incubation Catalytic potential after incubation (8 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) with organic solvent Dimethyl Sulfoxide (DMSO) 70% 197 210 IU/g.h Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer, 0.01 mM L-Cysteine Incubation: 8, Assay: 8 Incubation: 40Β°C, Assay: 40Β°C 0.05 mM Benzoyl-DL-arginine p-nitroanilide N-Ξ±-benzoyl-DL-arginine , p-Nitroaniline β€” Incubation: 220 rpm, Assay: 220 rpm Water-incubated control (in IU/g.h), assay in the presence of organic solvent
Activity + Stability - Incubation Catalytic potential after incubation (8 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) with organic solvent 1,1,1-Trichloroethane 50% 197 0 IU/g.h Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer, 0.01 mM L-Cysteine Incubation: 8, Assay: 8 Incubation: 40Β°C, Assay: 40Β°C 0.05 mM Benzoyl-DL-arginine p-nitroanilide N-Ξ±-benzoyl-DL-arginine , p-Nitroaniline β€” Incubation: 220 rpm, Assay: 220 rpm Water-incubated control (in IU/g.h), assay in the presence of organic solvent
Activity + Stability - Incubation Catalytic potential after incubation (8 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) with organic solvent Ethylene Glycol 70% 197 162 IU/g.h Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer, 0.01 mM L-Cysteine Incubation: 8, Assay: 8 Incubation: 40Β°C, Assay: 40Β°C 0.05 mM Benzoyl-DL-arginine p-nitroanilide N-Ξ±-benzoyl-DL-arginine , p-Nitroaniline β€” Incubation: 220 rpm, Assay: 220 rpm Water-incubated control (in IU/g.h), assay in the presence of organic solvent
Activity + Stability - Incubation Catalytic potential after incubation (8 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) with organic solvent Acetone 70% 197 127 IU/g.h Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer, 0.01 mM L-Cysteine Incubation: 8, Assay: 8 Incubation: 40Β°C, Assay: 40Β°C 0.05 mM Benzoyl-DL-arginine p-nitroanilide N-Ξ±-benzoyl-DL-arginine , p-Nitroaniline β€” Incubation: 220 rpm, Assay: 220 rpm Water-incubated control (in IU/g.h), assay in the presence of organic solvent
Activity + Stability - Incubation Catalytic potential after incubation (8 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) with organic solvent 2,2,2-Trifluoroethanol (TFE) 70% 197 0 IU/g.h Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer, 0.01 mM L-Cysteine Incubation: 8, Assay: 8 Incubation: 40Β°C, Assay: 40Β°C 0.05 mM Benzoyl-DL-arginine p-nitroanilide N-Ξ±-benzoyl-DL-arginine , p-Nitroaniline β€” Incubation: 220 rpm, Assay: 220 rpm Water-incubated control (in IU/g.h), assay in the presence of organic solvent
Activity + Stability - Incubation Catalytic potential after incubation (8 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) with organic solvent 1,1,1-Trichloroethane 70% 197 0 IU/g.h Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer, 0.01 mM L-Cysteine Incubation: 8, Assay: 8 Incubation: 40Β°C, Assay: 40Β°C 0.05 mM Benzoyl-DL-arginine p-nitroanilide N-Ξ±-benzoyl-DL-arginine , p-Nitroaniline β€” Incubation: 220 rpm, Assay: 220 rpm Water-incubated control (in IU/g.h), assay in the presence of organic solvent
Activity + Stability - Incubation Catalytic potential after incubation (8 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) with organic solvent 1-Octanol 70% 197 152 IU/g.h Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer, 0.01 mM L-Cysteine Incubation: 8, Assay: 8 Incubation: 40Β°C, Assay: 40Β°C 0.05 mM Benzoyl-DL-arginine p-nitroanilide N-Ξ±-benzoyl-DL-arginine , p-Nitroaniline β€” Incubation: 220 rpm, Assay: 220 rpm Water-incubated control (in IU/g.h), assay in the presence of organic solvent
Activity + Stability - Incubation Catalytic potential after incubation (8 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) with organic solvent Ethyl Acetate 70% 197 129 IU/g.h Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer, 0.01 mM L-Cysteine Incubation: 8, Assay: 8 Incubation: 40Β°C, Assay: 40Β°C 0.05 mM Benzoyl-DL-arginine p-nitroanilide N-Ξ±-benzoyl-DL-arginine , p-Nitroaniline β€” Incubation: 220 rpm, Assay: 220 rpm Water-incubated control (in IU/g.h), assay in the presence of organic solvent
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Visualization : Activity + Stability β€” Incubation

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Structure

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