Asclepain cI from Asclepias curassavica

Enzyme Description

Species
Asclepias curassavica β†—
Extremophile
No
EC Number
3.4.22.7 β†—

Sequence

Length: 194 amino acids
QGKCGSCWTFSAVASIETLIGIKEGRMIALSEQELLDCERTSFGCKGGYYANAFAYVAKNGITSRDRYPYIFQQGQCYQKEKVVKISGYRNVRRNDEKELQLVVAQQVVSIGIKSSSRDFQHYRQGIFNGACGPKLDHAVNIVGYGSEGGANYWIVRNSWGTGWGEGGYARLPMYSGQVGGYCGIVSQASYPVY
Anabella L. Origone et al. (2024) β€” Effect of Organic Solvents on the Activity, Stability and Secondary Structure of asclepain cI, Using FTIR and Molecular Dynamics Simulations
 Β· doi:10.1007/s10930-024-10182-4 β†—  Β· Activity + Stability - Incubation
51 measurements ▼
Database ID
UniProt: B7VF64 β†—
Sequence Annotation
Explicit - Provided UniProt Accesion Number
Protein Source
Purified, eukaryore Asclepias curassavica

Experimental Data (51 measurements)

51 measurements β€” page 2 of 3
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity + Stability - Incubation Catalytic potential after incubation (8 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) with organic solvent Dichloromethane 70% 197 177 IU/g.h Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer, 0.01 mM L-Cysteine Incubation: 8, Assay: 8 Incubation: 40Β°C, Assay: 40Β°C 0.05 mM Benzoyl-DL-arginine p-nitroanilide N-Ξ±-benzoyl-DL-arginine , p-Nitroaniline β€” Incubation: 220 rpm, Assay: 220 rpm Water-incubated control (in IU/g.h), assay in the presence of organic solvent
Activity + Stability - Incubation Catalytic potential after incubation (8 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) with organic solvent Diethyl Ether 70% 197 212 IU/g.h Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer, 0.01 mM L-Cysteine Incubation: 8, Assay: 8 Incubation: 40Β°C, Assay: 40Β°C 0.05 mM Benzoyl-DL-arginine p-nitroanilide N-Ξ±-benzoyl-DL-arginine , p-Nitroaniline β€” Incubation: 220 rpm, Assay: 220 rpm Water-incubated control (in IU/g.h), assay in the presence of organic solvent
Activity + Stability - Incubation Catalytic potential after incubation (8 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) with organic solvent n-Hexane 70% 197 163 IU/g.h Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer, 0.01 mM L-Cysteine Incubation: 8, Assay: 8 Incubation: 40Β°C, Assay: 40Β°C 0.05 mM Benzoyl-DL-arginine p-nitroanilide N-Ξ±-benzoyl-DL-arginine , p-Nitroaniline β€” Incubation: 220 rpm, Assay: 220 rpm Water-incubated control (in IU/g.h), assay in the presence of organic solvent
Activity + Stability - Incubation Catalytic potential after incubation (8 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) with organic solvent Chlorobenzene 70% 197 213 IU/g.h Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer, 0.01 mM L-Cysteine Incubation: 8, Assay: 8 Incubation: 40Β°C, Assay: 40Β°C 0.05 mM Benzoyl-DL-arginine p-nitroanilide N-Ξ±-benzoyl-DL-arginine , p-Nitroaniline β€” Incubation: 220 rpm, Assay: 220 rpm Water-incubated control (in IU/g.h), assay in the presence of organic solvent
Activity + Stability - Incubation Catalytic potential after incubation (8 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) with organic solvent Benzene 70% 197 203 IU/g.h Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer, 0.01 mM L-Cysteine Incubation: 8, Assay: 8 Incubation: 40Β°C, Assay: 40Β°C 0.05 mM Benzoyl-DL-arginine p-nitroanilide N-Ξ±-benzoyl-DL-arginine , p-Nitroaniline β€” Incubation: 220 rpm, Assay: 220 rpm Water-incubated control (in IU/g.h), assay in the presence of organic solvent
Activity + Stability - Incubation Catalytic potential after incubation (8 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) with organic solvent n-Hexadecane 70% 197 0 IU/g.h Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer, 0.01 mM L-Cysteine Incubation: 8, Assay: 8 Incubation: 40Β°C, Assay: 40Β°C 0.05 mM Benzoyl-DL-arginine p-nitroanilide N-Ξ±-benzoyl-DL-arginine , p-Nitroaniline β€” Incubation: 220 rpm, Assay: 220 rpm Water-incubated control (in IU/g.h), assay in the presence of organic solvent
Activity + Stability - Incubation Catalytic potential after incubation (8 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) with organic solvent n-Hexane 30% 197 217 IU/g.h Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer, 0.01 mM L-Cysteine Incubation: 8, Assay: 8 Incubation: 40Β°C, Assay: 40Β°C 0.05 mM Benzoyl-DL-arginine p-nitroanilide N-Ξ±-benzoyl-DL-arginine , p-Nitroaniline β€” Incubation: 220 rpm, Assay: 220 rpm Water-incubated control (in IU/g.h), assay in the presence of organic solvent
Activity + Stability - Incubation Catalytic potential after incubation (8 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) with organic solvent Acetonitrile 30% 197 199 IU/g.h Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer, 0.01 mM L-Cysteine Incubation: 8, Assay: 8 Incubation: 40Β°C, Assay: 40Β°C 0.05 mM Benzoyl-DL-arginine p-nitroanilide N-Ξ±-benzoyl-DL-arginine , p-Nitroaniline β€” Incubation: 220 rpm, Assay: 220 rpm Water-incubated control (in IU/g.h), assay in the presence of organic solvent
Activity + Stability - Incubation Catalytic potential after incubation (8 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) with organic solvent Methanol 30% 197 215 IU/g.h Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer, 0.01 mM L-Cysteine Incubation: 8, Assay: 8 Incubation: 40Β°C, Assay: 40Β°C 0.05 mM Benzoyl-DL-arginine p-nitroanilide N-Ξ±-benzoyl-DL-arginine , p-Nitroaniline β€” Incubation: 220 rpm, Assay: 220 rpm Water-incubated control (in IU/g.h), assay in the presence of organic solvent
Activity + Stability - Incubation Catalytic potential after incubation (8 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) with organic solvent Dimethyl Sulfoxide (DMSO) 30% 197 203 IU/g.h Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer, 0.01 mM L-Cysteine Incubation: 8, Assay: 8 Incubation: 40Β°C, Assay: 40Β°C 0.05 mM Benzoyl-DL-arginine p-nitroanilide N-Ξ±-benzoyl-DL-arginine , p-Nitroaniline β€” Incubation: 220 rpm, Assay: 220 rpm Water-incubated control (in IU/g.h), assay in the presence of organic solvent
Activity + Stability - Incubation Catalytic potential after incubation (8 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) with organic solvent Tetrahydrofuran (THF) 30% 197 203 IU/g.h Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer, 0.01 mM L-Cysteine Incubation: 8, Assay: 8 Incubation: 40Β°C, Assay: 40Β°C 0.05 mM Benzoyl-DL-arginine p-nitroanilide N-Ξ±-benzoyl-DL-arginine , p-Nitroaniline β€” Incubation: 220 rpm, Assay: 220 rpm Water-incubated control (in IU/g.h), assay in the presence of organic solvent
Activity + Stability - Incubation Catalytic potential after incubation (8 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) with organic solvent Ethylene Glycol 30% 197 207 IU/g.h Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer, 0.01 mM L-Cysteine Incubation: 8, Assay: 8 Incubation: 40Β°C, Assay: 40Β°C 0.05 mM Benzoyl-DL-arginine p-nitroanilide N-Ξ±-benzoyl-DL-arginine , p-Nitroaniline β€” Incubation: 220 rpm, Assay: 220 rpm Water-incubated control (in IU/g.h), assay in the presence of organic solvent
Activity + Stability - Incubation Catalytic potential after incubation (8 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) with organic solvent Acetone 30% 197 198 IU/g.h Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer, 0.01 mM L-Cysteine Incubation: 8, Assay: 8 Incubation: 40Β°C, Assay: 40Β°C 0.05 mM Benzoyl-DL-arginine p-nitroanilide N-Ξ±-benzoyl-DL-arginine , p-Nitroaniline β€” Incubation: 220 rpm, Assay: 220 rpm Water-incubated control (in IU/g.h), assay in the presence of organic solvent
Activity + Stability - Incubation Catalytic potential after incubation (8 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) with organic solvent 2,2,2-Trifluoroethanol (TFE) 30% 197 0 IU/g.h Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer, 0.01 mM L-Cysteine Incubation: 8, Assay: 8 Incubation: 40Β°C, Assay: 40Β°C 0.05 mM Benzoyl-DL-arginine p-nitroanilide N-Ξ±-benzoyl-DL-arginine , p-Nitroaniline β€” Incubation: 220 rpm, Assay: 220 rpm Water-incubated control (in IU/g.h), assay in the presence of organic solvent
Activity + Stability - Incubation Catalytic potential after incubation (8 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) with organic solvent 1,1,1-Trichloroethane 30% 197 0 IU/g.h Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer, 0.01 mM L-Cysteine Incubation: 8, Assay: 8 Incubation: 40Β°C, Assay: 40Β°C 0.05 mM Benzoyl-DL-arginine p-nitroanilide N-Ξ±-benzoyl-DL-arginine , p-Nitroaniline β€” Incubation: 220 rpm, Assay: 220 rpm Water-incubated control (in IU/g.h), assay in the presence of organic solvent
Activity + Stability - Incubation Catalytic potential after incubation (8 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) with organic solvent 1-Octanol 30% 197 218 IU/g.h Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer, 0.01 mM L-Cysteine Incubation: 8, Assay: 8 Incubation: 40Β°C, Assay: 40Β°C 0.05 mM Benzoyl-DL-arginine p-nitroanilide N-Ξ±-benzoyl-DL-arginine , p-Nitroaniline β€” Incubation: 220 rpm, Assay: 220 rpm Water-incubated control (in IU/g.h), assay in the presence of organic solvent
Activity + Stability - Incubation Catalytic potential after incubation (8 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) with organic solvent Ethyl Acetate 30% 197 216 IU/g.h Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer, 0.01 mM L-Cysteine Incubation: 8, Assay: 8 Incubation: 40Β°C, Assay: 40Β°C 0.05 mM Benzoyl-DL-arginine p-nitroanilide N-Ξ±-benzoyl-DL-arginine , p-Nitroaniline β€” Incubation: 220 rpm, Assay: 220 rpm Water-incubated control (in IU/g.h), assay in the presence of organic solvent
Activity + Stability - Incubation Catalytic potential after incubation (8 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) with organic solvent Dichloromethane 30% 197 212 IU/g.h Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer, 0.01 mM L-Cysteine Incubation: 8, Assay: 8 Incubation: 40Β°C, Assay: 40Β°C 0.05 mM Benzoyl-DL-arginine p-nitroanilide N-Ξ±-benzoyl-DL-arginine , p-Nitroaniline β€” Incubation: 220 rpm, Assay: 220 rpm Water-incubated control (in IU/g.h), assay in the presence of organic solvent
Activity + Stability - Incubation Catalytic potential after incubation (8 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) with organic solvent Diethyl Ether 30% 197 206 IU/g.h Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer, 0.01 mM L-Cysteine Incubation: 8, Assay: 8 Incubation: 40Β°C, Assay: 40Β°C 0.05 mM Benzoyl-DL-arginine p-nitroanilide N-Ξ±-benzoyl-DL-arginine , p-Nitroaniline β€” Incubation: 220 rpm, Assay: 220 rpm Water-incubated control (in IU/g.h), assay in the presence of organic solvent
Activity + Stability - Incubation Catalytic potential after incubation (8 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 410 nm) with organic solvent Ethanol 30% 197 207 IU/g.h Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer, 0.01 mM L-Cysteine Incubation: 8, Assay: 8 Incubation: 40Β°C, Assay: 40Β°C 0.05 mM Benzoyl-DL-arginine p-nitroanilide N-Ξ±-benzoyl-DL-arginine , p-Nitroaniline β€” Incubation: 220 rpm, Assay: 220 rpm Water-incubated control (in IU/g.h), assay in the presence of organic solvent
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Visualization : Activity + Stability β€” Incubation

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Structure

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