Phosphite dehydrogenase (gene Rs-PtxD) from Ralstonia sp. 4506
Enzyme Description
Sequence
MKPKVVLTHWVHPEIIELLSASADVIPNTTRETLPARSEVIARAKDADALMAFMPDSIDSAFLEECPKLRVIGAALKGYDNFDVNACTRHGVWLTIVPDLLTIPTAELTIGLLLGLTRHMLEGDRQIRSGHFQGWRPTLYGSGLTGKTLGIIGMGAVGRAIAQRLAGFEMNLLYCDPIPLNAEQEKAWHVQRVTLDELLEKCDYVVPMVPMAAETLHLIDATALAKMKTGSYLINACRGSVVDENAVIAALASGKLAGYAADVFEMEEWIRADRPQAIPKALLDNTAQTFFTPHLGSAVKEVRLEIERQAAMNIIQALAGEKPMGAINQPYPGVKAA
Gamal Nasser Abdel-Hady et al. (2023)
β A novel salt- and organic solvent-tolerant phosphite dehydrogenase from Cyanothece sp. ATCC 51142
Frontiers in Bioengineering and Biotechnology
Β· doi:10.3389/fbioe.2023.1255582 β
Β· Activity - Classical Stability - Incubation
▼
Database ID
NCBI: BAV60262.1 β
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host bacterium Escherichia coli BL21 pLysS
Experimental Data (40 measurements)
40 measurements β page 1 of 2
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Activity - Classical | Activity measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent | Dimethylformamide (DMF) | 20% | 100.0 | 123.0 | % | 20 mM MOPS buffer | 7.3 | 37Β°C | 1 mM Phosphite | Phosphate | 0.5 mM NAD+ | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent | Dimethylformamide (DMF) | 10% | 100.0 | 120.3 | % | 20 mM MOPS buffer | 7.3 | 37Β°C | 1 mM Phosphite | Phosphate | 0.5 mM NAD+ | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 50% | 100.0 | 29.8 | % | 20 mM MOPS buffer | 7.3 | 37Β°C | 1 mM Phosphite | Phosphate | 0.5 mM NAD+ | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 40% | 100.0 | 87.8 | % | 20 mM MOPS buffer | 7.3 | 37Β°C | 1 mM Phosphite | Phosphate | 0.5 mM NAD+ | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 30% | 100.0 | 118.7 | % | 20 mM MOPS buffer | 7.3 | 37Β°C | 1 mM Phosphite | Phosphate | 0.5 mM NAD+ | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 20% | 100.0 | 116.0 | % | 20 mM MOPS buffer | 7.3 | 37Β°C | 1 mM Phosphite | Phosphate | 0.5 mM NAD+ | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 10% | 100.0 | 110.6 | % | 20 mM MOPS buffer | 7.3 | 37Β°C | 1 mM Phosphite | Phosphate | 0.5 mM NAD+ | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent | Ethanol | 50% | 100.0 | 0.0 | % | 20 mM MOPS buffer | 7.3 | 37Β°C | 1 mM Phosphite | Phosphate | 0.5 mM NAD+ | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent | Ethanol | 40% | 100.0 | 4.3 | % | 20 mM MOPS buffer | 7.3 | 37Β°C | 1 mM Phosphite | Phosphate | 0.5 mM NAD+ | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent | Ethanol | 30% | 100.0 | 1.6 | % | 20 mM MOPS buffer | 7.3 | 37Β°C | 1 mM Phosphite | Phosphate | 0.5 mM NAD+ | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent | Ethanol | 20% | 100.0 | 119.8 | % | 20 mM MOPS buffer | 7.3 | 37Β°C | 1 mM Phosphite | Phosphate | 0.5 mM NAD+ | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent | Methanol | 50% | 100.0 | 0.5 | % | 20 mM MOPS buffer | 7.3 | 37Β°C | 1 mM Phosphite | Phosphate | 0.5 mM NAD+ | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent | Methanol | 40% | 100.0 | 1.6 | % | 20 mM MOPS buffer | 7.3 | 37Β°C | 1 mM Phosphite | Phosphate | 0.5 mM NAD+ | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent | Methanol | 30% | 100.0 | 69.9 | % | 20 mM MOPS buffer | 7.3 | 37Β°C | 1 mM Phosphite | Phosphate | 0.5 mM NAD+ | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent | Methanol | 20% | 100.0 | 107.8 | % | 20 mM MOPS buffer | 7.3 | 37Β°C | 1 mM Phosphite | Phosphate | 0.5 mM NAD+ | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent | Methanol | 10% | 100.0 | 105.7 | % | 20 mM MOPS buffer | 7.3 | 37Β°C | 1 mM Phosphite | Phosphate | 0.5 mM NAD+ | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent | Dimethylformamide (DMF) | 50% | 100.0 | 3.8 | % | 20 mM MOPS buffer | 7.3 | 37Β°C | 1 mM Phosphite | Phosphate | 0.5 mM NAD+ | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent | Dimethylformamide (DMF) | 40% | 100.0 | 0.0 | % | 20 mM MOPS buffer | 7.3 | 37Β°C | 1 mM Phosphite | Phosphate | 0.5 mM NAD+ | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent | Dimethylformamide (DMF) | 30% | 100.0 | 77.5 | % | 20 mM MOPS buffer | 7.3 | 37Β°C | 1 mM Phosphite | Phosphate | 0.5 mM NAD+ | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent | Ethanol | 10% | 100.0 | 112.2 | % | 20 mM MOPS buffer | 7.3 | 37Β°C | 1 mM Phosphite | Phosphate | 0.5 mM NAD+ | β | Classical aqueous control (in %) |
Visualization : Activity β Classical
One bar per measurement. Colour = solvent, shade = solvent volume.
Visualization : Stability β Incubation
One bar per measurement. Colour = solvent, shade = solvent volume.