Phosphite dehydrogenase (gene Rs-PtxD) from Ralstonia sp. 4506
Enzyme Description
Sequence
MKPKVVLTHWVHPEIIELLSASADVIPNTTRETLPARSEVIARAKDADALMAFMPDSIDSAFLEECPKLRVIGAALKGYDNFDVNACTRHGVWLTIVPDLLTIPTAELTIGLLLGLTRHMLEGDRQIRSGHFQGWRPTLYGSGLTGKTLGIIGMGAVGRAIAQRLAGFEMNLLYCDPIPLNAEQEKAWHVQRVTLDELLEKCDYVVPMVPMAAETLHLIDATALAKMKTGSYLINACRGSVVDENAVIAALASGKLAGYAADVFEMEEWIRADRPQAIPKALLDNTAQTFFTPHLGSAVKEVRLEIERQAAMNIIQALAGEKPMGAINQPYPGVKAA
Gamal Nasser Abdel-Hady et al. (2023)
β A novel salt- and organic solvent-tolerant phosphite dehydrogenase from Cyanothece sp. ATCC 51142
Frontiers in Bioengineering and Biotechnology
Β· doi:10.3389/fbioe.2023.1255582 β
Β· Activity - Classical Stability - Incubation
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Database ID
NCBI: BAV60262.1 β
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host bacterium Escherichia coli BL21 pLysS
Experimental Data (40 measurements)
40 measurements β page 2 of 2
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Stability - Incubation | Activity after incubation (6 hours at 25Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Ethanol | 10% | 100.0 | 93.0 | % | Incubation: 50 mM MOPS buffer, Assay: 20 mM MOPS buffer | Incubation: 7.3, Assay: 7.3 | Incubation: 25Β°C, Assay: 37Β°C | 1 mM Phosphite | Phosphate | Assay: 0.5 mM NAD+ | β | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (6 hours at 25Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Ethanol | 20% | 100.0 | 74.0 | % | Incubation: 50 mM MOPS buffer, Assay: 20 mM MOPS buffer | Incubation: 7.3, Assay: 7.3 | Incubation: 25Β°C, Assay: 37Β°C | 1 mM Phosphite | Phosphate | Assay: 0.5 mM NAD+ | β | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (6 hours at 25Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Ethanol | 30% | 100.0 | 0.0 | % | Incubation: 50 mM MOPS buffer, Assay: 20 mM MOPS buffer | Incubation: 7.3, Assay: 7.3 | Incubation: 25Β°C, Assay: 37Β°C | 1 mM Phosphite | Phosphate | Assay: 0.5 mM NAD+ | β | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (6 hours at 25Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Ethanol | 40% | 100.0 | 0.0 | % | Incubation: 50 mM MOPS buffer, Assay: 20 mM MOPS buffer | Incubation: 7.3, Assay: 7.3 | Incubation: 25Β°C, Assay: 37Β°C | 1 mM Phosphite | Phosphate | Assay: 0.5 mM NAD+ | β | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (6 hours at 25Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Ethanol | 50% | 100.0 | 0.0 | % | Incubation: 50 mM MOPS buffer, Assay: 20 mM MOPS buffer | Incubation: 7.3, Assay: 7.3 | Incubation: 25Β°C, Assay: 37Β°C | 1 mM Phosphite | Phosphate | Assay: 0.5 mM NAD+ | β | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (6 hours at 25Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 10% | 100.0 | 94.0 | % | Incubation: 50 mM MOPS buffer, Assay: 20 mM MOPS buffer | Incubation: 7.3, Assay: 7.3 | Incubation: 25Β°C, Assay: 37Β°C | 1 mM Phosphite | Phosphate | Assay: 0.5 mM NAD+ | β | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (6 hours at 25Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 20% | 100.0 | 85.0 | % | Incubation: 50 mM MOPS buffer, Assay: 20 mM MOPS buffer | Incubation: 7.3, Assay: 7.3 | Incubation: 25Β°C, Assay: 37Β°C | 1 mM Phosphite | Phosphate | Assay: 0.5 mM NAD+ | β | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (6 hours at 25Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 30% | 100.0 | 88.0 | % | Incubation: 50 mM MOPS buffer, Assay: 20 mM MOPS buffer | Incubation: 7.3, Assay: 7.3 | Incubation: 25Β°C, Assay: 37Β°C | 1 mM Phosphite | Phosphate | Assay: 0.5 mM NAD+ | β | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (6 hours at 25Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 40% | 100.0 | 91.0 | % | Incubation: 50 mM MOPS buffer, Assay: 20 mM MOPS buffer | Incubation: 7.3, Assay: 7.3 | Incubation: 25Β°C, Assay: 37Β°C | 1 mM Phosphite | Phosphate | Assay: 0.5 mM NAD+ | β | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (6 hours at 25Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 50% | 100.0 | 57.0 | % | Incubation: 50 mM MOPS buffer, Assay: 20 mM MOPS buffer | Incubation: 7.3, Assay: 7.3 | Incubation: 25Β°C, Assay: 37Β°C | 1 mM Phosphite | Phosphate | Assay: 0.5 mM NAD+ | β | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (6 hours at 25Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Methanol | 50% | 100.0 | 0.0 | % | Incubation: 50 mM MOPS buffer, Assay: 20 mM MOPS buffer | Incubation: 7.3, Assay: 7.3 | Incubation: 25Β°C, Assay: 37Β°C | 1 mM Phosphite | Phosphate | Assay: 0.5 mM NAD+ | β | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (6 hours at 25Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Methanol | 40% | 100.0 | 0.0 | % | Incubation: 50 mM MOPS buffer, Assay: 20 mM MOPS buffer | Incubation: 7.3, Assay: 7.3 | Incubation: 25Β°C, Assay: 37Β°C | 1 mM Phosphite | Phosphate | Assay: 0.5 mM NAD+ | β | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (6 hours at 25Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Methanol | 30% | 100.0 | 60.0 | % | Incubation: 50 mM MOPS buffer, Assay: 20 mM MOPS buffer | Incubation: 7.3, Assay: 7.3 | Incubation: 25Β°C, Assay: 37Β°C | 1 mM Phosphite | Phosphate | Assay: 0.5 mM NAD+ | β | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (6 hours at 25Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Methanol | 20% | 100.0 | 83.0 | % | Incubation: 50 mM MOPS buffer, Assay: 20 mM MOPS buffer | Incubation: 7.3, Assay: 7.3 | Incubation: 25Β°C, Assay: 37Β°C | 1 mM Phosphite | Phosphate | Assay: 0.5 mM NAD+ | β | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (6 hours at 25Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Methanol | 10% | 100.0 | 91.0 | % | Incubation: 50 mM MOPS buffer, Assay: 20 mM MOPS buffer | Incubation: 7.3, Assay: 7.3 | Incubation: 25Β°C, Assay: 37Β°C | 1 mM Phosphite | Phosphate | Assay: 0.5 mM NAD+ | β | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (6 hours at 25Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Dimethylformamide (DMF) | 50% | 100.0 | 1.0 | % | Incubation: 50 mM MOPS buffer, Assay: 20 mM MOPS buffer | Incubation: 7.3, Assay: 7.3 | Incubation: 25Β°C, Assay: 37Β°C | 1 mM Phosphite | Phosphate | Assay: 0.5 mM NAD+ | β | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (6 hours at 25Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Dimethylformamide (DMF) | 40% | 100.0 | 6.0 | % | Incubation: 50 mM MOPS buffer, Assay: 20 mM MOPS buffer | Incubation: 7.3, Assay: 7.3 | Incubation: 25Β°C, Assay: 37Β°C | 1 mM Phosphite | Phosphate | Assay: 0.5 mM NAD+ | β | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (6 hours at 25Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Dimethylformamide (DMF) | 30% | 100.0 | 63.0 | % | Incubation: 50 mM MOPS buffer, Assay: 20 mM MOPS buffer | Incubation: 7.3, Assay: 7.3 | Incubation: 25Β°C, Assay: 37Β°C | 1 mM Phosphite | Phosphate | Assay: 0.5 mM NAD+ | β | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (6 hours at 25Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Dimethylformamide (DMF) | 20% | 100.0 | 95.0 | % | Incubation: 50 mM MOPS buffer, Assay: 20 mM MOPS buffer | Incubation: 7.3, Assay: 7.3 | Incubation: 25Β°C, Assay: 37Β°C | 1 mM Phosphite | Phosphate | Assay: 0.5 mM NAD+ | β | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (6 hours at 25Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Dimethylformamide (DMF) | 10% | 100.0 | 100.0 | % | Incubation: 50 mM MOPS buffer, Assay: 20 mM MOPS buffer | Incubation: 7.3, Assay: 7.3 | Incubation: 25Β°C, Assay: 37Β°C | 1 mM Phosphite | Phosphate | Assay: 0.5 mM NAD+ | β | Non-incubated control (in %), assay in aqueous phase |
Visualization : Activity β Classical
One bar per measurement. Colour = solvent, shade = solvent volume.
Visualization : Stability β Incubation
One bar per measurement. Colour = solvent, shade = solvent volume.