Lipase from Monascus pilosus isolate F15
Enzyme Description
Species
Extremophile
Yes
"acidophilic" organism
EC Number
Sequence
MDTLAGPNITDKQTILTFAKMTQNDYVEEPGTEDWEDINGPFNYSRSFGWQGDGLRGHIYADKENSTIVISLKGTSPALFDGAGTTTNDKVNDNLYFSCCCGQGGSYLWRQVCDCQKSPYTANLTCIIESMTDENRYYRAAIDLYSNVTELYPNSSVWLTGHSLGGAMTSLLGLTFGLPVVTFEAVPEALPAARLGLPSPPGNDPRLPQKRQYTGSYHFGHTADPVYMGTCNGVNSVCTWGGYALESACHTGQMCVYDTVGDKGWRVGMGTHRIKAVIRDVLEAYDDVPPCAPEEECYDCELWKFFRSNGSESTTTTTTTSTTSTTTSTTLTATRTSTCETPGWWGCLDETTTTTTASMTTTTSTCKTPGWFGCKDPTTTTSATLTPPGTTTPSTTTTTTTSCADPGWFGCRDPTSTVTATSTCETPGYFWGCLDTPTNSPHRITSGPTPPPTITPSGGRDMCTSRAFLGFVCV
Mohamed M.M. Ahmed et al. (2025)
β Optimization, characterization, and molecular modelling of an acid and organic solvent-tolerant lipase isolated from Monascus pilosus as a potential detergent additive
International Journal of Biological Macromolecules
Β· doi:10.1016/j.ijbiomac.2025.144883 β
Β· Stability - Incubation
▼
Database ID
UniParc: UPI003D5ACE49 β
Sequence Annotation
Explicit - Provided
Protein Source
Purified, fungus Moascus pilosus strain F15 (produced by natural fungal expression using submerged fermentation)
Experimental Data (30 measurements)
30 measurements β page 1 of 2
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Stability - Incubation | Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase | Ethanol | 10% | 100.0 | 78.3 | % | Incubation: ?, Assay: 50 mM Tris-HCl buffer, 0.1% gum arabic, 0.4% Triton X-100 | Incubation: ?, Assay: 8 | Incubation: 30Β°C, Assay: 40Β°C | 0.3 Mg/ml p-Nitrophenyl palmitate | p-Nitrophenol , Palmitic Acid | β | β | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase | Acetic Acid | 30% | 100.0 | 2.5 | % | Incubation: ?, Assay: 50 mM Tris-HCl buffer, 0.1% gum arabic, 0.4% Triton X-100 | Incubation: ?, Assay: 8 | Incubation: 30Β°C, Assay: 40Β°C | 0.3 Mg/ml p-Nitrophenyl palmitate | p-Nitrophenol , Palmitic Acid | β | β | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase | Acetic Acid | 20% | 100.0 | 4.9 | % | Incubation: ?, Assay: 50 mM Tris-HCl buffer, 0.1% gum arabic, 0.4% Triton X-100 | Incubation: ?, Assay: 8 | Incubation: 30Β°C, Assay: 40Β°C | 0.3 Mg/ml p-Nitrophenyl palmitate | p-Nitrophenol , Palmitic Acid | β | β | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase | Acetic Acid | 10% | 100.0 | 7.2 | % | Incubation: ?, Assay: 50 mM Tris-HCl buffer, 0.1% gum arabic, 0.4% Triton X-100 | Incubation: ?, Assay: 8 | Incubation: 30Β°C, Assay: 40Β°C | 0.3 Mg/ml p-Nitrophenyl palmitate | p-Nitrophenol , Palmitic Acid | β | β | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 30% | 100.0 | 55.7 | % | Incubation: ?, Assay: 50 mM Tris-HCl buffer, 0.1% gum arabic, 0.4% Triton X-100 | Incubation: ?, Assay: 8 | Incubation: 30Β°C, Assay: 40Β°C | 0.3 Mg/ml p-Nitrophenyl palmitate | p-Nitrophenol , Palmitic Acid | β | β | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 20% | 100.0 | 84.8 | % | Incubation: ?, Assay: 50 mM Tris-HCl buffer, 0.1% gum arabic, 0.4% Triton X-100 | Incubation: ?, Assay: 8 | Incubation: 30Β°C, Assay: 40Β°C | 0.3 Mg/ml p-Nitrophenyl palmitate | p-Nitrophenol , Palmitic Acid | β | β | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 10% | 100.0 | 88.4 | % | Incubation: ?, Assay: 50 mM Tris-HCl buffer, 0.1% gum arabic, 0.4% Triton X-100 | Incubation: ?, Assay: 8 | Incubation: 30Β°C, Assay: 40Β°C | 0.3 Mg/ml p-Nitrophenyl palmitate | p-Nitrophenol , Palmitic Acid | β | β | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase | Toluene | 30% | 100.0 | 71.2 | % | Incubation: ?, Assay: 50 mM Tris-HCl buffer, 0.1% gum arabic, 0.4% Triton X-100 | Incubation: ?, Assay: 8 | Incubation: 30Β°C, Assay: 40Β°C | 0.3 Mg/ml p-Nitrophenyl palmitate | p-Nitrophenol , Palmitic Acid | β | β | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase | Toluene | 20% | 100.0 | 83.3 | % | Incubation: ?, Assay: 50 mM Tris-HCl buffer, 0.1% gum arabic, 0.4% Triton X-100 | Incubation: ?, Assay: 8 | Incubation: 30Β°C, Assay: 40Β°C | 0.3 Mg/ml p-Nitrophenyl palmitate | p-Nitrophenol , Palmitic Acid | β | β | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase | Toluene | 10% | 100.0 | 89.9 | % | Incubation: ?, Assay: 50 mM Tris-HCl buffer, 0.1% gum arabic, 0.4% Triton X-100 | Incubation: ?, Assay: 8 | Incubation: 30Β°C, Assay: 40Β°C | 0.3 Mg/ml p-Nitrophenyl palmitate | p-Nitrophenol , Palmitic Acid | β | β | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase | n-Hexane | 30% | 100.0 | 51.0 | % | Incubation: ?, Assay: 50 mM Tris-HCl buffer, 0.1% gum arabic, 0.4% Triton X-100 | Incubation: ?, Assay: 8 | Incubation: 30Β°C, Assay: 40Β°C | 0.3 Mg/ml p-Nitrophenyl palmitate | p-Nitrophenol , Palmitic Acid | β | β | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase | n-Hexane | 20% | 100.0 | 71.7 | % | Incubation: ?, Assay: 50 mM Tris-HCl buffer, 0.1% gum arabic, 0.4% Triton X-100 | Incubation: ?, Assay: 8 | Incubation: 30Β°C, Assay: 40Β°C | 0.3 Mg/ml p-Nitrophenyl palmitate | p-Nitrophenol , Palmitic Acid | β | β | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase | n-Hexane | 10% | 100.0 | 84.8 | % | Incubation: ?, Assay: 50 mM Tris-HCl buffer, 0.1% gum arabic, 0.4% Triton X-100 | Incubation: ?, Assay: 8 | Incubation: 30Β°C, Assay: 40Β°C | 0.3 Mg/ml p-Nitrophenyl palmitate | p-Nitrophenol , Palmitic Acid | β | β | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase | Ethanol | 30% | 100.0 | 13.1 | % | Incubation: ?, Assay: 50 mM Tris-HCl buffer, 0.1% gum arabic, 0.4% Triton X-100 | Incubation: ?, Assay: 8 | Incubation: 30Β°C, Assay: 40Β°C | 0.3 Mg/ml p-Nitrophenyl palmitate | p-Nitrophenol , Palmitic Acid | β | β | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase | Ethanol | 20% | 100.0 | 50.4 | % | Incubation: ?, Assay: 50 mM Tris-HCl buffer, 0.1% gum arabic, 0.4% Triton X-100 | Incubation: ?, Assay: 8 | Incubation: 30Β°C, Assay: 40Β°C | 0.3 Mg/ml p-Nitrophenyl palmitate | p-Nitrophenol , Palmitic Acid | β | β | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase | Chloroform | 10% | 100.0 | 92.0 | % | Incubation: ?, Assay: 50 mM Tris-HCl buffer, 0.1% gum arabic, 0.4% Triton X-100 | Incubation: ?, Assay: 8 | Incubation: 30Β°C, Assay: 40Β°C | 0.3 Mg/ml p-Nitrophenyl palmitate | p-Nitrophenol , Palmitic Acid | β | β | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase | Acetonitrile | 30% | 100.0 | 3.0 | % | Incubation: ?, Assay: 50 mM Tris-HCl buffer, 0.1% gum arabic, 0.4% Triton X-100 | Incubation: ?, Assay: 8 | Incubation: 30Β°C, Assay: 40Β°C | 0.3 Mg/ml p-Nitrophenyl palmitate | p-Nitrophenol , Palmitic Acid | β | β | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase | Acetonitrile | 20% | 100.0 | 19.8 | % | Incubation: ?, Assay: 50 mM Tris-HCl buffer, 0.1% gum arabic, 0.4% Triton X-100 | Incubation: ?, Assay: 8 | Incubation: 30Β°C, Assay: 40Β°C | 0.3 Mg/ml p-Nitrophenyl palmitate | p-Nitrophenol , Palmitic Acid | β | β | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase | Acetonitrile | 10% | 100.0 | 49.8 | % | Incubation: ?, Assay: 50 mM Tris-HCl buffer, 0.1% gum arabic, 0.4% Triton X-100 | Incubation: ?, Assay: 8 | Incubation: 30Β°C, Assay: 40Β°C | 0.3 Mg/ml p-Nitrophenyl palmitate | p-Nitrophenol , Palmitic Acid | β | β | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase | Acetone | 30% | 100.0 | 19.4 | % | Incubation: ?, Assay: 50 mM Tris-HCl buffer, 0.1% gum arabic, 0.4% Triton X-100 | Incubation: ?, Assay: 8 | Incubation: 30Β°C, Assay: 40Β°C | 0.3 Mg/ml p-Nitrophenyl palmitate | p-Nitrophenol , Palmitic Acid | β | β | Non-incubated control (in %), assay in aqueous phase |
Visualization : Stability β Incubation
One bar per measurement. Colour = solvent, shade = solvent volume.