Lipase from Monascus pilosus isolate F15

Enzyme Description

Species

Monascus pilosus isolate F15 ↗

Extremophile

Yes "acidophilic" organism

EC Number

3.1.1.3 ↗

Sequence

Length: 474 amino acids

MDTLAGPNITDKQTILTFAKMTQNDYVEEPGTEDWEDINGPFNYSRSFGWQGDGLRGHIYADKENSTIVISLKGTSPALFDGAGTTTNDKVNDNLYFSCCCGQGGSYLWRQVCDCQKSPYTANLTCIIESMTDENRYYRAAIDLYSNVTELYPNSSVWLTGHSLGGAMTSLLGLTFGLPVVTFEAVPEALPAARLGLPSPPGNDPRLPQKRQYTGSYHFGHTADPVYMGTCNGVNSVCTWGGYALESACHTGQMCVYDTVGDKGWRVGMGTHRIKAVIRDVLEAYDDVPPCAPEEECYDCELWKFFRSNGSESTTTTTTTSTTSTTTSTTLTATRTSTCETPGWWGCLDETTTTTTASMTTTTSTCKTPGWFGCKDPTTTTSATLTPPGTTTPSTTTTTTTSCADPGWFGCRDPTSTVTATSTCETPGYFWGCLDTPTNSPHRITSGPTPPPTITPSGGRDMCTSRAFLGFVCV

Mohamed M.M. Ahmed et al. (2025) — Optimization, characterization, and molecular modelling of an acid and organic solvent-tolerant lipase isolated from Monascus pilosus as a potential detergent additive

International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2025.144883 ↗ · Stability - Incubation

30 measurements ▼

Database ID

UniParc: UPI003D5ACE49 ↗

Sequence Annotation

Explicit - Provided

Protein Source

Purified, fungus Moascus pilosus strain F15 (produced by natural fungal expression using submerged fermentation)

Experimental Data (30 measurements)

30 measurements — page 2 of 2

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Stability - Incubation	Activity after incubation (30 minutes at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Acetone	20%	100.0	39.4	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 0.1% gum arabic, 0.4% Triton X-100	Incubation: ?, Assay: 8	Incubation: 30°C, Assay: 40°C	0.3 Mg/ml p-Nitrophenyl palmitate	p-Nitrophenol , Palmitic Acid	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Acetone	10%	100.0	73.8	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 0.1% gum arabic, 0.4% Triton X-100	Incubation: ?, Assay: 8	Incubation: 30°C, Assay: 40°C	0.3 Mg/ml p-Nitrophenyl palmitate	p-Nitrophenol , Palmitic Acid	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Isopropanol	30%	100.0	4.8	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 0.1% gum arabic, 0.4% Triton X-100	Incubation: ?, Assay: 8	Incubation: 30°C, Assay: 40°C	0.3 Mg/ml p-Nitrophenyl palmitate	p-Nitrophenol , Palmitic Acid	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Isopropanol	20%	100.0	32.3	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 0.1% gum arabic, 0.4% Triton X-100	Incubation: ?, Assay: 8	Incubation: 30°C, Assay: 40°C	0.3 Mg/ml p-Nitrophenyl palmitate	p-Nitrophenol , Palmitic Acid	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Isopropanol	10%	100.0	81.9	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 0.1% gum arabic, 0.4% Triton X-100	Incubation: ?, Assay: 8	Incubation: 30°C, Assay: 40°C	0.3 Mg/ml p-Nitrophenyl palmitate	p-Nitrophenol , Palmitic Acid	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Methanol	30%	100.0	7.0	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 0.1% gum arabic, 0.4% Triton X-100	Incubation: ?, Assay: 8	Incubation: 30°C, Assay: 40°C	0.3 Mg/ml p-Nitrophenyl palmitate	p-Nitrophenol , Palmitic Acid	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Methanol	20%	100.0	60.4	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 0.1% gum arabic, 0.4% Triton X-100	Incubation: ?, Assay: 8	Incubation: 30°C, Assay: 40°C	0.3 Mg/ml p-Nitrophenyl palmitate	p-Nitrophenol , Palmitic Acid	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Methanol	10%	100.0	71.9	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 0.1% gum arabic, 0.4% Triton X-100	Incubation: ?, Assay: 8	Incubation: 30°C, Assay: 40°C	0.3 Mg/ml p-Nitrophenyl palmitate	p-Nitrophenol , Palmitic Acid	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Chloroform	30%	100.0	48.1	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 0.1% gum arabic, 0.4% Triton X-100	Incubation: ?, Assay: 8	Incubation: 30°C, Assay: 40°C	0.3 Mg/ml p-Nitrophenyl palmitate	p-Nitrophenol , Palmitic Acid	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Chloroform	20%	100.0	68.8	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 0.1% gum arabic, 0.4% Triton X-100	Incubation: ?, Assay: 8	Incubation: 30°C, Assay: 40°C	0.3 Mg/ml p-Nitrophenyl palmitate	p-Nitrophenol , Palmitic Acid	—	—	Non-incubated control (in %), assay in aqueous phase

Stability - Incubation	Activity after incubation (30 minutes at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Ethanol	10%	100.0	78.3	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 0.1% gum arabic, 0.4% Triton X-100	Incubation: ?, Assay: 8	Incubation: 30°C, Assay: 40°C	0.3 Mg/ml p-Nitrophenyl palmitate	p-Nitrophenol, Palmitic Acid	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Acetic Acid	30%	100.0	2.5	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 0.1% gum arabic, 0.4% Triton X-100	Incubation: ?, Assay: 8	Incubation: 30°C, Assay: 40°C	0.3 Mg/ml p-Nitrophenyl palmitate	p-Nitrophenol, Palmitic Acid	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Acetic Acid	20%	100.0	4.9	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 0.1% gum arabic, 0.4% Triton X-100	Incubation: ?, Assay: 8	Incubation: 30°C, Assay: 40°C	0.3 Mg/ml p-Nitrophenyl palmitate	p-Nitrophenol, Palmitic Acid	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Acetic Acid	10%	100.0	7.2	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 0.1% gum arabic, 0.4% Triton X-100	Incubation: ?, Assay: 8	Incubation: 30°C, Assay: 40°C	0.3 Mg/ml p-Nitrophenyl palmitate	p-Nitrophenol, Palmitic Acid	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	30%	100.0	55.7	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 0.1% gum arabic, 0.4% Triton X-100	Incubation: ?, Assay: 8	Incubation: 30°C, Assay: 40°C	0.3 Mg/ml p-Nitrophenyl palmitate	p-Nitrophenol, Palmitic Acid	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	20%	100.0	84.8	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 0.1% gum arabic, 0.4% Triton X-100	Incubation: ?, Assay: 8	Incubation: 30°C, Assay: 40°C	0.3 Mg/ml p-Nitrophenyl palmitate	p-Nitrophenol, Palmitic Acid	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	10%	100.0	88.4	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 0.1% gum arabic, 0.4% Triton X-100	Incubation: ?, Assay: 8	Incubation: 30°C, Assay: 40°C	0.3 Mg/ml p-Nitrophenyl palmitate	p-Nitrophenol, Palmitic Acid	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Toluene	30%	100.0	71.2	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 0.1% gum arabic, 0.4% Triton X-100	Incubation: ?, Assay: 8	Incubation: 30°C, Assay: 40°C	0.3 Mg/ml p-Nitrophenyl palmitate	p-Nitrophenol, Palmitic Acid	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Toluene	20%	100.0	83.3	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 0.1% gum arabic, 0.4% Triton X-100	Incubation: ?, Assay: 8	Incubation: 30°C, Assay: 40°C	0.3 Mg/ml p-Nitrophenyl palmitate	p-Nitrophenol, Palmitic Acid	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Toluene	10%	100.0	89.9	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 0.1% gum arabic, 0.4% Triton X-100	Incubation: ?, Assay: 8	Incubation: 30°C, Assay: 40°C	0.3 Mg/ml p-Nitrophenyl palmitate	p-Nitrophenol, Palmitic Acid	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	n-Hexane	30%	100.0	51.0	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 0.1% gum arabic, 0.4% Triton X-100	Incubation: ?, Assay: 8	Incubation: 30°C, Assay: 40°C	0.3 Mg/ml p-Nitrophenyl palmitate	p-Nitrophenol, Palmitic Acid	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	n-Hexane	20%	100.0	71.7	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 0.1% gum arabic, 0.4% Triton X-100	Incubation: ?, Assay: 8	Incubation: 30°C, Assay: 40°C	0.3 Mg/ml p-Nitrophenyl palmitate	p-Nitrophenol, Palmitic Acid	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	n-Hexane	10%	100.0	84.8	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 0.1% gum arabic, 0.4% Triton X-100	Incubation: ?, Assay: 8	Incubation: 30°C, Assay: 40°C	0.3 Mg/ml p-Nitrophenyl palmitate	p-Nitrophenol, Palmitic Acid	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Ethanol	30%	100.0	13.1	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 0.1% gum arabic, 0.4% Triton X-100	Incubation: ?, Assay: 8	Incubation: 30°C, Assay: 40°C	0.3 Mg/ml p-Nitrophenyl palmitate	p-Nitrophenol, Palmitic Acid	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Ethanol	20%	100.0	50.4	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 0.1% gum arabic, 0.4% Triton X-100	Incubation: ?, Assay: 8	Incubation: 30°C, Assay: 40°C	0.3 Mg/ml p-Nitrophenyl palmitate	p-Nitrophenol, Palmitic Acid	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Chloroform	10%	100.0	92.0	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 0.1% gum arabic, 0.4% Triton X-100	Incubation: ?, Assay: 8	Incubation: 30°C, Assay: 40°C	0.3 Mg/ml p-Nitrophenyl palmitate	p-Nitrophenol, Palmitic Acid	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Acetonitrile	30%	100.0	3.0	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 0.1% gum arabic, 0.4% Triton X-100	Incubation: ?, Assay: 8	Incubation: 30°C, Assay: 40°C	0.3 Mg/ml p-Nitrophenyl palmitate	p-Nitrophenol, Palmitic Acid	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Acetonitrile	20%	100.0	19.8	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 0.1% gum arabic, 0.4% Triton X-100	Incubation: ?, Assay: 8	Incubation: 30°C, Assay: 40°C	0.3 Mg/ml p-Nitrophenyl palmitate	p-Nitrophenol, Palmitic Acid	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Acetonitrile	10%	100.0	49.8	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 0.1% gum arabic, 0.4% Triton X-100	Incubation: ?, Assay: 8	Incubation: 30°C, Assay: 40°C	0.3 Mg/ml p-Nitrophenyl palmitate	p-Nitrophenol, Palmitic Acid	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Acetone	30%	100.0	19.4	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 0.1% gum arabic, 0.4% Triton X-100	Incubation: ?, Assay: 8	Incubation: 30°C, Assay: 40°C	0.3 Mg/ml p-Nitrophenyl palmitate	p-Nitrophenol, Palmitic Acid	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Acetone	20%	100.0	39.4	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 0.1% gum arabic, 0.4% Triton X-100	Incubation: ?, Assay: 8	Incubation: 30°C, Assay: 40°C	0.3 Mg/ml p-Nitrophenyl palmitate	p-Nitrophenol, Palmitic Acid	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Acetone	10%	100.0	73.8	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 0.1% gum arabic, 0.4% Triton X-100	Incubation: ?, Assay: 8	Incubation: 30°C, Assay: 40°C	0.3 Mg/ml p-Nitrophenyl palmitate	p-Nitrophenol, Palmitic Acid	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Isopropanol	30%	100.0	4.8	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 0.1% gum arabic, 0.4% Triton X-100	Incubation: ?, Assay: 8	Incubation: 30°C, Assay: 40°C	0.3 Mg/ml p-Nitrophenyl palmitate	p-Nitrophenol, Palmitic Acid	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Isopropanol	20%	100.0	32.3	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 0.1% gum arabic, 0.4% Triton X-100	Incubation: ?, Assay: 8	Incubation: 30°C, Assay: 40°C	0.3 Mg/ml p-Nitrophenyl palmitate	p-Nitrophenol, Palmitic Acid	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Isopropanol	10%	100.0	81.9	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 0.1% gum arabic, 0.4% Triton X-100	Incubation: ?, Assay: 8	Incubation: 30°C, Assay: 40°C	0.3 Mg/ml p-Nitrophenyl palmitate	p-Nitrophenol, Palmitic Acid	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Methanol	30%	100.0	7.0	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 0.1% gum arabic, 0.4% Triton X-100	Incubation: ?, Assay: 8	Incubation: 30°C, Assay: 40°C	0.3 Mg/ml p-Nitrophenyl palmitate	p-Nitrophenol, Palmitic Acid	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Methanol	20%	100.0	60.4	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 0.1% gum arabic, 0.4% Triton X-100	Incubation: ?, Assay: 8	Incubation: 30°C, Assay: 40°C	0.3 Mg/ml p-Nitrophenyl palmitate	p-Nitrophenol, Palmitic Acid	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Methanol	10%	100.0	71.9	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 0.1% gum arabic, 0.4% Triton X-100	Incubation: ?, Assay: 8	Incubation: 30°C, Assay: 40°C	0.3 Mg/ml p-Nitrophenyl palmitate	p-Nitrophenol, Palmitic Acid	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Chloroform	30%	100.0	48.1	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 0.1% gum arabic, 0.4% Triton X-100	Incubation: ?, Assay: 8	Incubation: 30°C, Assay: 40°C	0.3 Mg/ml p-Nitrophenyl palmitate	p-Nitrophenol, Palmitic Acid	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Chloroform	20%	100.0	68.8	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 0.1% gum arabic, 0.4% Triton X-100	Incubation: ?, Assay: 8	Incubation: 30°C, Assay: 40°C	0.3 Mg/ml p-Nitrophenyl palmitate	p-Nitrophenol, Palmitic Acid	—	—	Non-incubated control (in %), assay in aqueous phase

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Visualization : Stability — Incubation

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