Organophosphate hydrolase (gene opdH) from Arthrobacter sp. HM01
Enzyme Description
Sequence
MITISEAGFTLTHEHICGSSAGFLRAWPEFFGSRKALAEKAVRGLRRARAAGVRTIVDVSTFDIGRDVSLLAEVSRAADVHIVAATGLWFDPPLSMRLRSVEELTQFFLREIQYGIEDTGIRAGIIKVATTGKATPFQELVLKAAARASLATGVPVTTHTAASQRDGEQQAAIFESEGLSPSRVCIGHSDDTDDLSYLTALAARGYLIGLDHIPHSAIGLEDNASASALLGIRSWQTRALLIKALIDQGYMKQILVSNDWLFGFSSYVTNIMDVM
Himanshu Mali et al. (2022)
β A novel organophosphate hydrolase from Arthrobacter sp. HM01: Characterization and applications
Bioresource Technology
Β· doi:10.1016/j.biortech.2022.126870 β
Β· Activity - Classical Stability - Half-life
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Database ID
NCBI: MW413755.1 β
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host bacterium Escherichia coli BL21
Experimental Data (39 measurements)
39 measurements β page 1 of 2
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent | Methanol | 50% | 100.0 | 65.0 | % | 30 mM PBS buffer | 7 | 25Β°C | 5 Β΅gram/ml Paraoxon | diethyl phosphate , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent | Chloroform | 50% | 100.0 | 31.0 | % | 30 mM PBS buffer | 7 | 25Β°C | 5 Β΅gram/ml Paraoxon | diethyl phosphate , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent | Chloroform | 10% | 100.0 | 85.0 | % | 30 mM PBS buffer | 7 | 25Β°C | 5 Β΅gram/ml Paraoxon | diethyl phosphate , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent | Xylene | 50% | 100.0 | 35.0 | % | 30 mM PBS buffer | 7 | 25Β°C | 5 Β΅gram/ml Paraoxon | diethyl phosphate , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent | Xylene | 10% | 100.0 | 73.0 | % | 30 mM PBS buffer | 7 | 25Β°C | 5 Β΅gram/ml Paraoxon | diethyl phosphate , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent | Petroleum Ether | 50% | 100.0 | 56.0 | % | 30 mM PBS buffer | 7 | 25Β°C | 5 Β΅gram/ml Paraoxon | diethyl phosphate , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent | Petroleum Ether | 10% | 100.0 | 72.0 | % | 30 mM PBS buffer | 7 | 25Β°C | 5 Β΅gram/ml Paraoxon | diethyl phosphate , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 50% | 100.0 | 29.0 | % | 30 mM PBS buffer | 7 | 25Β°C | 5 Β΅gram/ml Paraoxon | diethyl phosphate , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 10% | 100.0 | 84.0 | % | 30 mM PBS buffer | 7 | 25Β°C | 5 Β΅gram/ml Paraoxon | diethyl phosphate , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent | Acetonitrile | 50% | 100.0 | 63.0 | % | 30 mM PBS buffer | 7 | 25Β°C | 5 Β΅gram/ml Paraoxon | diethyl phosphate , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent | Acetonitrile | 10% | 100.0 | 91.0 | % | 30 mM PBS buffer | 7 | 25Β°C | 5 Β΅gram/ml Paraoxon | diethyl phosphate , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent | n-Hexane | 50% | 100.0 | 32.0 | % | 30 mM PBS buffer | 7 | 25Β°C | 5 Β΅gram/ml Paraoxon | diethyl phosphate , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent | n-Hexane | 10% | 100.0 | 82.0 | % | 30 mM PBS buffer | 7 | 25Β°C | 5 Β΅gram/ml Paraoxon | diethyl phosphate , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent | Benzene | 10% | 100.0 | 71.0 | % | 30 mM PBS buffer | 7 | 25Β°C | 5 Β΅gram/ml Paraoxon | diethyl phosphate , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent | Benzene | 50% | 100.0 | 27.0 | % | 30 mM PBS buffer | 7 | 25Β°C | 5 Β΅gram/ml Paraoxon | diethyl phosphate , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent | Methanol | 10% | 100.0 | 83.0 | % | 30 mM PBS buffer | 7 | 25Β°C | 5 Β΅gram/ml Paraoxon | diethyl phosphate , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent | Ethanol | 10% | 100.0 | 90.0 | % | 30 mM PBS buffer | 7 | 25Β°C | 5 Β΅gram/ml Paraoxon | diethyl phosphate , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent | Ethanol | 50% | 100.0 | 44.0 | % | 30 mM PBS buffer | 7 | 25Β°C | 5 Β΅gram/ml Paraoxon | diethyl phosphate , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent | 1-Propanol | 10% | 100.0 | 81.0 | % | 30 mM PBS buffer | 7 | 25Β°C | 5 Β΅gram/ml Paraoxon | diethyl phosphate , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent | 1-Propanol | 50% | 100.0 | 53.0 | % | 30 mM PBS buffer | 7 | 25Β°C | 5 Β΅gram/ml Paraoxon | diethyl phosphate , p-Nitrophenol | β | β | Classical aqueous control (in %) |
Visualization : Activity β Classical
One bar per measurement. Colour = solvent, shade = solvent volume.
Visualization : Stability β Half-life
One bar per measurement. Colour = solvent, shade = solvent volume.