Organophosphate hydrolase (gene opdH) from Arthrobacter sp. HM01
Enzyme Description
Sequence
MITISEAGFTLTHEHICGSSAGFLRAWPEFFGSRKALAEKAVRGLRRARAAGVRTIVDVSTFDIGRDVSLLAEVSRAADVHIVAATGLWFDPPLSMRLRSVEELTQFFLREIQYGIEDTGIRAGIIKVATTGKATPFQELVLKAAARASLATGVPVTTHTAASQRDGEQQAAIFESEGLSPSRVCIGHSDDTDDLSYLTALAARGYLIGLDHIPHSAIGLEDNASASALLGIRSWQTRALLIKALIDQGYMKQILVSNDWLFGFSSYVTNIMDVM
Himanshu Mali et al. (2022)
β A novel organophosphate hydrolase from Arthrobacter sp. HM01: Characterization and applications
Bioresource Technology
Β· doi:10.1016/j.biortech.2022.126870 β
Β· Activity - Classical Stability - Half-life
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Database ID
NCBI: MW413755.1 β
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host bacterium Escherichia coli BL21
Experimental Data (39 measurements)
39 measurements β page 2 of 2
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent | Isoamyl Alcohol | 10% | 100.0 | 81.0 | % | 30 mM PBS buffer | 7 | 25Β°C | 5 Β΅gram/ml Paraoxon | diethyl phosphate , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent | Isoamyl Alcohol | 50% | 100.0 | 43.0 | % | 30 mM PBS buffer | 7 | 25Β°C | 5 Β΅gram/ml Paraoxon | diethyl phosphate , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent | Acetone | 10% | 100.0 | 90.0 | % | 30 mM PBS buffer | 7 | 25Β°C | 5 Β΅gram/ml Paraoxon | diethyl phosphate , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent | Acetone | 50% | 100.0 | 31.0 | % | 30 mM PBS buffer | 7 | 25Β°C | 5 Β΅gram/ml Paraoxon | diethyl phosphate , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent | Ethyl Acetate | 10% | 100.0 | 94.0 | % | 30 mM PBS buffer | 7 | 25Β°C | 5 Β΅gram/ml Paraoxon | diethyl phosphate , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent | Ethyl Acetate | 50% | 100.0 | 70.0 | % | 30 mM PBS buffer | 7 | 25Β°C | 5 Β΅gram/ml Paraoxon | diethyl phosphate , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Stability - Half-life | Half-life, measured by activity assay by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase | Chloroform | 50% | 30.0 | 0.45 | hours | Incubation: 30 mM PBS buffer, Assay: 30 mM PBS buffer | Incubation: 7, Assay: 7 | Incubation: 25Β°C, Assay: 25Β°C | 5 Β΅gram/ml Paraoxon | diethyl phosphate , p-Nitrophenol | β | β | Classical aqueous control (in hours) | Uncertainty about whether assay really was in aqueous phase |
| Stability - Half-life | Half-life, measured by activity assay by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase | Xylene | 50% | 30.0 | 0.2 | hours | Incubation: 30 mM PBS buffer, Assay: 30 mM PBS buffer | Incubation: 7, Assay: 7 | Incubation: 25Β°C, Assay: 25Β°C | 5 Β΅gram/ml Paraoxon | diethyl phosphate , p-Nitrophenol | β | β | Classical aqueous control (in hours) | Uncertainty about whether assay really was in aqueous phase |
| Stability - Half-life | Half-life, measured by activity assay by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase | Petroleum Ether | 50% | 30.0 | 0.5 | hours | Incubation: 30 mM PBS buffer, Assay: 30 mM PBS buffer | Incubation: 7, Assay: 7 | Incubation: 25Β°C, Assay: 25Β°C | 5 Β΅gram/ml Paraoxon | diethyl phosphate , p-Nitrophenol | β | β | Classical aqueous control (in hours) | Uncertainty about whether assay really was in aqueous phase |
| Stability - Half-life | Half-life, measured by activity assay by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 50% | 30.0 | 0.6 | hours | Incubation: 30 mM PBS buffer, Assay: 30 mM PBS buffer | Incubation: 7, Assay: 7 | Incubation: 25Β°C, Assay: 25Β°C | 5 Β΅gram/ml Paraoxon | diethyl phosphate , p-Nitrophenol | β | β | Classical aqueous control (in hours) | Uncertainty about whether assay really was in aqueous phase |
| Stability - Half-life | Half-life, measured by activity assay by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase | Acetonitrile | 50% | 30.0 | 0.76 | hours | Incubation: 30 mM PBS buffer, Assay: 30 mM PBS buffer | Incubation: 7, Assay: 7 | Incubation: 25Β°C, Assay: 25Β°C | 5 Β΅gram/ml Paraoxon | diethyl phosphate , p-Nitrophenol | β | β | Classical aqueous control (in hours) | Uncertainty about whether assay really was in aqueous phase |
| Stability - Half-life | Half-life, measured by activity assay by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase | n-Hexane | 50% | 30.0 | 0.3 | hours | Incubation: 30 mM PBS buffer, Assay: 30 mM PBS buffer | Incubation: 7, Assay: 7 | Incubation: 25Β°C, Assay: 25Β°C | 5 Β΅gram/ml Paraoxon | diethyl phosphate , p-Nitrophenol | β | β | Classical aqueous control (in hours) | Uncertainty about whether assay really was in aqueous phase |
| Stability - Half-life | Half-life, measured by activity assay by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase | Benzene | 50% | 30.0 | 0.2 | hours | Incubation: 30 mM PBS buffer, Assay: 30 mM PBS buffer | Incubation: 7, Assay: 7 | Incubation: 25Β°C, Assay: 25Β°C | 5 Β΅gram/ml Paraoxon | diethyl phosphate , p-Nitrophenol | β | β | Classical aqueous control (in hours) | Uncertainty about whether assay really was in aqueous phase |
| Stability - Half-life | Half-life, measured by activity assay by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase | Ethyl Acetate | 50% | 30.0 | 0.7 | hours | Incubation: 30 mM PBS buffer, Assay: 30 mM PBS buffer | Incubation: 7, Assay: 7 | Incubation: 25Β°C, Assay: 25Β°C | 5 Β΅gram/ml Paraoxon | diethyl phosphate , p-Nitrophenol | β | β | Classical aqueous control (in hours) | Uncertainty about whether assay really was in aqueous phase |
| Stability - Half-life | Half-life, measured by activity assay by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase | Acetone | 50% | 30.0 | 0.5 | hours | Incubation: 30 mM PBS buffer, Assay: 30 mM PBS buffer | Incubation: 7, Assay: 7 | Incubation: 25Β°C, Assay: 25Β°C | 5 Β΅gram/ml Paraoxon | diethyl phosphate , p-Nitrophenol | β | β | Classical aqueous control (in hours) | Uncertainty about whether assay really was in aqueous phase |
| Stability - Half-life | Half-life, measured by activity assay by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase | Isoamyl Alcohol | 50% | 30.0 | 0.45 | hours | Incubation: 30 mM PBS buffer, Assay: 30 mM PBS buffer | Incubation: 7, Assay: 7 | Incubation: 25Β°C, Assay: 25Β°C | 5 Β΅gram/ml Paraoxon | diethyl phosphate , p-Nitrophenol | β | β | Classical aqueous control (in hours) | Uncertainty about whether assay really was in aqueous phase |
| Stability - Half-life | Half-life, measured by activity assay by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase | 1-Propanol | 50% | 30.0 | 0.65 | hours | Incubation: 30 mM PBS buffer, Assay: 30 mM PBS buffer | Incubation: 7, Assay: 7 | Incubation: 25Β°C, Assay: 25Β°C | 5 Β΅gram/ml Paraoxon | diethyl phosphate , p-Nitrophenol | β | β | Classical aqueous control (in hours) | Uncertainty about whether assay really was in aqueous phase |
| Stability - Half-life | Half-life, measured by activity assay by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase | Ethanol | 50% | 30.0 | 0.5 | hours | Incubation: 30 mM PBS buffer, Assay: 30 mM PBS buffer | Incubation: 7, Assay: 7 | Incubation: 25Β°C, Assay: 25Β°C | 5 Β΅gram/ml Paraoxon | diethyl phosphate , p-Nitrophenol | β | β | Classical aqueous control (in hours) | Uncertainty about whether assay really was in aqueous phase |
| Stability - Half-life | Half-life, measured by activity assay by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase | Methanol | 50% | 30.0 | 0.81 | hours | Incubation: 30 mM PBS buffer, Assay: 30 mM PBS buffer | Incubation: 7, Assay: 7 | Incubation: 25Β°C, Assay: 25Β°C | 5 Β΅gram/ml Paraoxon | diethyl phosphate , p-Nitrophenol | β | β | Classical aqueous control (in hours) | Uncertainty about whether assay really was in aqueous phase |
Visualization : Activity β Classical
One bar per measurement. Colour = solvent, shade = solvent volume.
Visualization : Stability β Half-life
One bar per measurement. Colour = solvent, shade = solvent volume.