Lipase from Bacillus licheniformis NCU CS-5

Enzyme Description

Species
Bacillus licheniformis NCU CS-5 β†—
Extremophile
No
EC Number
3.1.1.3 β†—

Sequence

Length: 204 amino acids
MRRHSFLSILLICMLSVVSVFSFRPSAASAASHNPVVMVHGIGGADYNFIGIKSYLQSQGWTSSELYAINFIDKTGNNINNAPRLSEYIKRVLNQTGASKVDIVAHSMGGANTLYYIKNLDGADKVGHVVTLGGANRLVTNTAPQNDKISYTSIYSTSDYIVLNSLSKLDGANNVQISGVSHVGLLFSSKVNALIKDGLTASGK
Junxin Zhao et al. (2022) β€” Expression and characterization of a novel lipase from Bacillus licheniformis NCU CS-5 for application in enhancing fatty acids flavor release for low-fat cheeses
Food Chemistry  Β· doi:10.1016/j.foodchem.2021.130868 β†—  Β· Stability - Incubation
48 measurements ▼
Database ID
UniProt: Q65HR4 β†—
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (48 measurements)

48 measurements β€” page 1 of 3
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (3 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Glycerol 100% 100.0 119.82 % Incubation: ?, Assay: 40 mM Tris-HCl buffer, 10% isopropanol Incubation: ?, Assay: 7 Incubation: 40Β°C, Assay: 40Β°C 1 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (3 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 75% 100.0 116.92 % Incubation: ?, Assay: 40 mM Tris-HCl buffer, 10% isopropanol Incubation: ?, Assay: 7 Incubation: 40Β°C, Assay: 40Β°C 1 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (3 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Methanol 75% 100.0 107.93 % Incubation: ?, Assay: 40 mM Tris-HCl buffer, 10% isopropanol Incubation: ?, Assay: 7 Incubation: 40Β°C, Assay: 40Β°C 1 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (3 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Ethanol 75% 100.0 70.93 % Incubation: ?, Assay: 40 mM Tris-HCl buffer, 10% isopropanol Incubation: ?, Assay: 7 Incubation: 40Β°C, Assay: 40Β°C 1 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (3 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Acetone 75% 100.0 72.84 % Incubation: ?, Assay: 40 mM Tris-HCl buffer, 10% isopropanol Incubation: ?, Assay: 7 Incubation: 40Β°C, Assay: 40Β°C 1 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (3 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Acetonitrile 75% 100.0 84.89 % Incubation: ?, Assay: 40 mM Tris-HCl buffer, 10% isopropanol Incubation: ?, Assay: 7 Incubation: 40Β°C, Assay: 40Β°C 1 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (3 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Isopropanol 75% 100.0 64.36 % Incubation: ?, Assay: 40 mM Tris-HCl buffer, 10% isopropanol Incubation: ?, Assay: 7 Incubation: 40Β°C, Assay: 40Β°C 1 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (3 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Ethyl Acetate 75% 100.0 69.08 % Incubation: ?, Assay: 40 mM Tris-HCl buffer, 10% isopropanol Incubation: ?, Assay: 7 Incubation: 40Β°C, Assay: 40Β°C 1 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (3 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Chloroform 75% 100.0 65.96 % Incubation: ?, Assay: 40 mM Tris-HCl buffer, 10% isopropanol Incubation: ?, Assay: 7 Incubation: 40Β°C, Assay: 40Β°C 1 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (3 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase n-Hexane 75% 100.0 93.12 % Incubation: ?, Assay: 40 mM Tris-HCl buffer, 10% isopropanol Incubation: ?, Assay: 7 Incubation: 40Β°C, Assay: 40Β°C 1 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (3 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Petroleum Ether 75% 100.0 111.45 % Incubation: ?, Assay: 40 mM Tris-HCl buffer, 10% isopropanol Incubation: ?, Assay: 7 Incubation: 40Β°C, Assay: 40Β°C 1 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (3 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase n-Heptane 75% 100.0 86.55 % Incubation: ?, Assay: 40 mM Tris-HCl buffer, 10% isopropanol Incubation: ?, Assay: 7 Incubation: 40Β°C, Assay: 40Β°C 1 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (3 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Glycerol 75% 100.0 115.21 % Incubation: ?, Assay: 40 mM Tris-HCl buffer, 10% isopropanol Incubation: ?, Assay: 7 Incubation: 40Β°C, Assay: 40Β°C 1 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (3 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 100% 100.0 125.04 % Incubation: ?, Assay: 40 mM Tris-HCl buffer, 10% isopropanol Incubation: ?, Assay: 7 Incubation: 40Β°C, Assay: 40Β°C 1 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (3 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Methanol 100% 100.0 100.5 % Incubation: ?, Assay: 40 mM Tris-HCl buffer, 10% isopropanol Incubation: ?, Assay: 7 Incubation: 40Β°C, Assay: 40Β°C 1 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (3 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Ethanol 100% 100.0 59.16 % Incubation: ?, Assay: 40 mM Tris-HCl buffer, 10% isopropanol Incubation: ?, Assay: 7 Incubation: 40Β°C, Assay: 40Β°C 1 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (3 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Acetone 100% 100.0 80.12 % Incubation: ?, Assay: 40 mM Tris-HCl buffer, 10% isopropanol Incubation: ?, Assay: 7 Incubation: 40Β°C, Assay: 40Β°C 1 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (3 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Acetonitrile 100% 100.0 85.79 % Incubation: ?, Assay: 40 mM Tris-HCl buffer, 10% isopropanol Incubation: ?, Assay: 7 Incubation: 40Β°C, Assay: 40Β°C 1 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (3 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Isopropanol 100% 100.0 74.96 % Incubation: ?, Assay: 40 mM Tris-HCl buffer, 10% isopropanol Incubation: ?, Assay: 7 Incubation: 40Β°C, Assay: 40Β°C 1 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (3 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Ethyl Acetate 100% 100.0 60.63 % Incubation: ?, Assay: 40 mM Tris-HCl buffer, 10% isopropanol Incubation: ?, Assay: 7 Incubation: 40Β°C, Assay: 40Β°C 1 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
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Visualization : Stability β€” Incubation

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Structure

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