Lipase from Bacillus licheniformis NCU CS-5

Enzyme Description

Species
Bacillus licheniformis NCU CS-5 β†—
Extremophile
No
EC Number
3.1.1.3 β†—

Sequence

Length: 204 amino acids
MRRHSFLSILLICMLSVVSVFSFRPSAASAASHNPVVMVHGIGGADYNFIGIKSYLQSQGWTSSELYAINFIDKTGNNINNAPRLSEYIKRVLNQTGASKVDIVAHSMGGANTLYYIKNLDGADKVGHVVTLGGANRLVTNTAPQNDKISYTSIYSTSDYIVLNSLSKLDGANNVQISGVSHVGLLFSSKVNALIKDGLTASGK
Junxin Zhao et al. (2022) β€” Expression and characterization of a novel lipase from Bacillus licheniformis NCU CS-5 for application in enhancing fatty acids flavor release for low-fat cheeses
Food Chemistry  Β· doi:10.1016/j.foodchem.2021.130868 β†—  Β· Stability - Incubation
48 measurements ▼
Database ID
UniProt: Q65HR4 β†—
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (48 measurements)

48 measurements β€” page 2 of 3
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (3 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Chloroform 100% 100.0 59.47 % Incubation: ?, Assay: 40 mM Tris-HCl buffer, 10% isopropanol Incubation: ?, Assay: 7 Incubation: 40Β°C, Assay: 40Β°C 1 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (3 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase n-Hexane 100% 100.0 96.22 % Incubation: ?, Assay: 40 mM Tris-HCl buffer, 10% isopropanol Incubation: ?, Assay: 7 Incubation: 40Β°C, Assay: 40Β°C 1 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (3 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Petroleum Ether 100% 100.0 112.22 % Incubation: ?, Assay: 40 mM Tris-HCl buffer, 10% isopropanol Incubation: ?, Assay: 7 Incubation: 40Β°C, Assay: 40Β°C 1 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (3 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase n-Heptane 100% 100.0 97.67 % Incubation: ?, Assay: 40 mM Tris-HCl buffer, 10% isopropanol Incubation: ?, Assay: 7 Incubation: 40Β°C, Assay: 40Β°C 1 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (3 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Glycerol 50% 100.0 89.54 % Incubation: ?, Assay: 40 mM Tris-HCl buffer, 10% isopropanol Incubation: ?, Assay: 7 Incubation: 40Β°C, Assay: 40Β°C 1 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (3 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 25% 100.0 139.95 % Incubation: ?, Assay: 40 mM Tris-HCl buffer, 10% isopropanol Incubation: ?, Assay: 7 Incubation: 40Β°C, Assay: 40Β°C 1 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (3 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Methanol 25% 100.0 134.48 % Incubation: ?, Assay: 40 mM Tris-HCl buffer, 10% isopropanol Incubation: ?, Assay: 7 Incubation: 40Β°C, Assay: 40Β°C 1 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (3 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Ethanol 25% 100.0 128.0 % Incubation: ?, Assay: 40 mM Tris-HCl buffer, 10% isopropanol Incubation: ?, Assay: 7 Incubation: 40Β°C, Assay: 40Β°C 1 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (3 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Acetone 25% 100.0 124.44 % Incubation: ?, Assay: 40 mM Tris-HCl buffer, 10% isopropanol Incubation: ?, Assay: 7 Incubation: 40Β°C, Assay: 40Β°C 1 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (3 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Acetonitrile 25% 100.0 115.28 % Incubation: ?, Assay: 40 mM Tris-HCl buffer, 10% isopropanol Incubation: ?, Assay: 7 Incubation: 40Β°C, Assay: 40Β°C 1 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (3 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Isopropanol 25% 100.0 94.23 % Incubation: ?, Assay: 40 mM Tris-HCl buffer, 10% isopropanol Incubation: ?, Assay: 7 Incubation: 40Β°C, Assay: 40Β°C 1 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (3 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Ethyl Acetate 25% 100.0 77.88 % Incubation: ?, Assay: 40 mM Tris-HCl buffer, 10% isopropanol Incubation: ?, Assay: 7 Incubation: 40Β°C, Assay: 40Β°C 1 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (3 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Chloroform 25% 100.0 118.49 % Incubation: ?, Assay: 40 mM Tris-HCl buffer, 10% isopropanol Incubation: ?, Assay: 7 Incubation: 40Β°C, Assay: 40Β°C 1 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (3 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase n-Hexane 25% 100.0 123.78 % Incubation: ?, Assay: 40 mM Tris-HCl buffer, 10% isopropanol Incubation: ?, Assay: 7 Incubation: 40Β°C, Assay: 40Β°C 1 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (3 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Petroleum Ether 25% 100.0 137.63 % Incubation: ?, Assay: 40 mM Tris-HCl buffer, 10% isopropanol Incubation: ?, Assay: 7 Incubation: 40Β°C, Assay: 40Β°C 1 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (3 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase n-Heptane 25% 100.0 118.37 % Incubation: ?, Assay: 40 mM Tris-HCl buffer, 10% isopropanol Incubation: ?, Assay: 7 Incubation: 40Β°C, Assay: 40Β°C 1 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (3 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Glycerol 25% 100.0 135.2 % Incubation: ?, Assay: 40 mM Tris-HCl buffer, 10% isopropanol Incubation: ?, Assay: 7 Incubation: 40Β°C, Assay: 40Β°C 1 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (3 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 50% 100.0 97.01 % Incubation: ?, Assay: 40 mM Tris-HCl buffer, 10% isopropanol Incubation: ?, Assay: 7 Incubation: 40Β°C, Assay: 40Β°C 1 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (3 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Methanol 50% 100.0 92.92 % Incubation: ?, Assay: 40 mM Tris-HCl buffer, 10% isopropanol Incubation: ?, Assay: 7 Incubation: 40Β°C, Assay: 40Β°C 1 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (3 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Ethanol 50% 100.0 76.31 % Incubation: ?, Assay: 40 mM Tris-HCl buffer, 10% isopropanol Incubation: ?, Assay: 7 Incubation: 40Β°C, Assay: 40Β°C 1 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
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Visualization : Stability β€” Incubation

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Structure

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