Esterase (gene EstCS1) from a compost metagenomic library
Enzyme Description
Species
Na (compost metagenomic library)
Extremophile
No
no indication for organism but "moderately thermophilic" and "moderately alkaliphilic" enzyme
EC Number
Sequence
MSIHPSIQEVLNQVPLDLPRTLTPELIKELRETRVPLPEELRPKIYKKEDQIIQGPNGDIPIRLYTPKEQEENYIGVIAFHGGGWSLGNIESHDGAFRQLANVSGAKVISVDYRLAPENPFPAGLEDCYAVLKWVCENAESLQIDPEKIVVAGDSAGGNLSASLTQMARDRNGPKIWKQVLIYPATDALRSIEDSPYESVRENADAPILSASVTKSFWDHYISTEDDANNPYCSPIRANSFENLPPALIITAQYDPIRDEGEAYGKKLMENGVPTIIRRVEGAIHGFITMPIPFAYEILEEIGKFIREK
Ji-Min Park et al. (2020)
β Characterization of a Novel Moderately Thermophilic Solvent-Tolerant Esterase Isolated From a Compost Metagenome Library
Frontiers in Microbiology
Β· doi:10.3389/fmicb.2019.03069 β
Β· Stability - Incubation
▼
Database ID
UniParc: UPI00022FC99C β
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)
Experimental Data (28 measurements)
28 measurements β page 1 of 2
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Stability - Incubation | Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase | Methanol | 30% | 100.0 | 90.8 | % | Incubation: 100 mM GTA buffer, Assay: 100 mM GTA buffer, 4% Ethanol, 1% acetonitrile | Incubation: 9, Assay: 9 | Incubation: 30Β°C, Assay: 25Β°C | 0.1 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase | Dimethylformamide (DMF) | 50% | 100.0 | 51.2 | % | Incubation: 100 mM GTA buffer, Assay: 100 mM GTA buffer, 4% Ethanol, 1% acetonitrile | Incubation: 9, Assay: 9 | Incubation: 30Β°C, Assay: 25Β°C | 0.1 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase | Acetone | 50% | 100.0 | 81.8 | % | Incubation: 100 mM GTA buffer, Assay: 100 mM GTA buffer, 4% Ethanol, 1% acetonitrile | Incubation: 9, Assay: 9 | Incubation: 30Β°C, Assay: 25Β°C | 0.1 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 50% | 100.0 | 66.1 | % | Incubation: 100 mM GTA buffer, Assay: 100 mM GTA buffer, 4% Ethanol, 1% acetonitrile | Incubation: 9, Assay: 9 | Incubation: 30Β°C, Assay: 25Β°C | 0.1 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase | Acetonitrile | 50% | 100.0 | 43.0 | % | Incubation: 100 mM GTA buffer, Assay: 100 mM GTA buffer, 4% Ethanol, 1% acetonitrile | Incubation: 9, Assay: 9 | Incubation: 30Β°C, Assay: 25Β°C | 0.1 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase | Isopropanol | 50% | 100.0 | 25.2 | % | Incubation: 100 mM GTA buffer, Assay: 100 mM GTA buffer, 4% Ethanol, 1% acetonitrile | Incubation: 9, Assay: 9 | Incubation: 30Β°C, Assay: 25Β°C | 0.1 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase | Ethanol | 50% | 100.0 | 50.3 | % | Incubation: 100 mM GTA buffer, Assay: 100 mM GTA buffer, 4% Ethanol, 1% acetonitrile | Incubation: 9, Assay: 9 | Incubation: 30Β°C, Assay: 25Β°C | 0.1 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase | Methanol | 50% | 100.0 | 43.4 | % | Incubation: 100 mM GTA buffer, Assay: 100 mM GTA buffer, 4% Ethanol, 1% acetonitrile | Incubation: 9, Assay: 9 | Incubation: 30Β°C, Assay: 25Β°C | 0.1 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase | Dimethylformamide (DMF) | 30% | 100.0 | 87.4 | % | Incubation: 100 mM GTA buffer, Assay: 100 mM GTA buffer, 4% Ethanol, 1% acetonitrile | Incubation: 9, Assay: 9 | Incubation: 30Β°C, Assay: 25Β°C | 0.1 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase | Acetone | 30% | 100.0 | 100.0 | % | Incubation: 100 mM GTA buffer, Assay: 100 mM GTA buffer, 4% Ethanol, 1% acetonitrile | Incubation: 9, Assay: 9 | Incubation: 30Β°C, Assay: 25Β°C | 0.1 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 30% | 100.0 | 74.4 | % | Incubation: 100 mM GTA buffer, Assay: 100 mM GTA buffer, 4% Ethanol, 1% acetonitrile | Incubation: 9, Assay: 9 | Incubation: 30Β°C, Assay: 25Β°C | 0.1 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase | Acetonitrile | 30% | 100.0 | 101.1 | % | Incubation: 100 mM GTA buffer, Assay: 100 mM GTA buffer, 4% Ethanol, 1% acetonitrile | Incubation: 9, Assay: 9 | Incubation: 30Β°C, Assay: 25Β°C | 0.1 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase | Isopropanol | 30% | 100.0 | 115.8 | % | Incubation: 100 mM GTA buffer, Assay: 100 mM GTA buffer, 4% Ethanol, 1% acetonitrile | Incubation: 9, Assay: 9 | Incubation: 30Β°C, Assay: 25Β°C | 0.1 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase | Ethanol | 30% | 100.0 | 101.4 | % | Incubation: 100 mM GTA buffer, Assay: 100 mM GTA buffer, 4% Ethanol, 1% acetonitrile | Incubation: 9, Assay: 9 | Incubation: 30Β°C, Assay: 25Β°C | 0.1 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase | Methanol | 10% | 100.0 | 95.0 | % | Incubation: 100 mM GTA buffer, Assay: 100 mM GTA buffer, 4% Ethanol, 1% acetonitrile | Incubation: 9, Assay: 9 | Incubation: 30Β°C, Assay: 25Β°C | 0.1 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase | Dimethylformamide (DMF) | 20% | 100.0 | 96.8 | % | Incubation: 100 mM GTA buffer, Assay: 100 mM GTA buffer, 4% Ethanol, 1% acetonitrile | Incubation: 9, Assay: 9 | Incubation: 30Β°C, Assay: 25Β°C | 0.1 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase | Acetone | 20% | 100.0 | 103.9 | % | Incubation: 100 mM GTA buffer, Assay: 100 mM GTA buffer, 4% Ethanol, 1% acetonitrile | Incubation: 9, Assay: 9 | Incubation: 30Β°C, Assay: 25Β°C | 0.1 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 20% | 100.0 | 86.0 | % | Incubation: 100 mM GTA buffer, Assay: 100 mM GTA buffer, 4% Ethanol, 1% acetonitrile | Incubation: 9, Assay: 9 | Incubation: 30Β°C, Assay: 25Β°C | 0.1 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase | Acetonitrile | 20% | 100.0 | 110.5 | % | Incubation: 100 mM GTA buffer, Assay: 100 mM GTA buffer, 4% Ethanol, 1% acetonitrile | Incubation: 9, Assay: 9 | Incubation: 30Β°C, Assay: 25Β°C | 0.1 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase | Isopropanol | 20% | 100.0 | 111.2 | % | Incubation: 100 mM GTA buffer, Assay: 100 mM GTA buffer, 4% Ethanol, 1% acetonitrile | Incubation: 9, Assay: 9 | Incubation: 30Β°C, Assay: 25Β°C | 0.1 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase |
Visualization : Stability β Incubation
One bar per measurement. Colour = solvent, shade = solvent volume.