Esterase (gene EstCS1) from a compost metagenomic library

Enzyme Description

Species

Na (compost metagenomic library)

Extremophile

No no indication for organism but "moderately thermophilic" and "moderately alkaliphilic" enzyme

EC Number

3.1.1.1 ↗

Sequence

Length: 309 amino acids

MSIHPSIQEVLNQVPLDLPRTLTPELIKELRETRVPLPEELRPKIYKKEDQIIQGPNGDIPIRLYTPKEQEENYIGVIAFHGGGWSLGNIESHDGAFRQLANVSGAKVISVDYRLAPENPFPAGLEDCYAVLKWVCENAESLQIDPEKIVVAGDSAGGNLSASLTQMARDRNGPKIWKQVLIYPATDALRSIEDSPYESVRENADAPILSASVTKSFWDHYISTEDDANNPYCSPIRANSFENLPPALIITAQYDPIRDEGEAYGKKLMENGVPTIIRRVEGAIHGFITMPIPFAYEILEEIGKFIREK

Ji-Min Park et al. (2020) — Characterization of a Novel Moderately Thermophilic Solvent-Tolerant Esterase Isolated From a Compost Metagenome Library

Frontiers in Microbiology · doi:10.3389/fmicb.2019.03069 ↗ · Stability - Incubation

28 measurements ▼

Database ID

UniParc: UPI00022FC99C ↗

Sequence Annotation

Explicit - Provided GenBank Accession Number

Protein Source

Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (28 measurements)

28 measurements — page 2 of 2

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Ethanol	20%	100.0	99.9	%	Incubation: 100 mM GTA buffer, Assay: 100 mM GTA buffer, 4% Ethanol, 1% acetonitrile	Incubation: 9, Assay: 9	Incubation: 30°C, Assay: 25°C	0.1 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Methanol	20%	100.0	92.7	%	Incubation: 100 mM GTA buffer, Assay: 100 mM GTA buffer, 4% Ethanol, 1% acetonitrile	Incubation: 9, Assay: 9	Incubation: 30°C, Assay: 25°C	0.1 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Dimethylformamide (DMF)	10%	100.0	91.6	%	Incubation: 100 mM GTA buffer, Assay: 100 mM GTA buffer, 4% Ethanol, 1% acetonitrile	Incubation: 9, Assay: 9	Incubation: 30°C, Assay: 25°C	0.1 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Acetone	10%	100.0	109.4	%	Incubation: 100 mM GTA buffer, Assay: 100 mM GTA buffer, 4% Ethanol, 1% acetonitrile	Incubation: 9, Assay: 9	Incubation: 30°C, Assay: 25°C	0.1 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	10%	100.0	91.8	%	Incubation: 100 mM GTA buffer, Assay: 100 mM GTA buffer, 4% Ethanol, 1% acetonitrile	Incubation: 9, Assay: 9	Incubation: 30°C, Assay: 25°C	0.1 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Acetonitrile	10%	100.0	95.1	%	Incubation: 100 mM GTA buffer, Assay: 100 mM GTA buffer, 4% Ethanol, 1% acetonitrile	Incubation: 9, Assay: 9	Incubation: 30°C, Assay: 25°C	0.1 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Isopropanol	10%	100.0	119.0	%	Incubation: 100 mM GTA buffer, Assay: 100 mM GTA buffer, 4% Ethanol, 1% acetonitrile	Incubation: 9, Assay: 9	Incubation: 30°C, Assay: 25°C	0.1 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Ethanol	10%	100.0	97.8	%	Incubation: 100 mM GTA buffer, Assay: 100 mM GTA buffer, 4% Ethanol, 1% acetonitrile	Incubation: 9, Assay: 9	Incubation: 30°C, Assay: 25°C	0.1 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase

Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Methanol	30%	100.0	90.8	%	Incubation: 100 mM GTA buffer, Assay: 100 mM GTA buffer, 4% Ethanol, 1% acetonitrile	Incubation: 9, Assay: 9	Incubation: 30°C, Assay: 25°C	0.1 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Dimethylformamide (DMF)	50%	100.0	51.2	%	Incubation: 100 mM GTA buffer, Assay: 100 mM GTA buffer, 4% Ethanol, 1% acetonitrile	Incubation: 9, Assay: 9	Incubation: 30°C, Assay: 25°C	0.1 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Acetone	50%	100.0	81.8	%	Incubation: 100 mM GTA buffer, Assay: 100 mM GTA buffer, 4% Ethanol, 1% acetonitrile	Incubation: 9, Assay: 9	Incubation: 30°C, Assay: 25°C	0.1 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	50%	100.0	66.1	%	Incubation: 100 mM GTA buffer, Assay: 100 mM GTA buffer, 4% Ethanol, 1% acetonitrile	Incubation: 9, Assay: 9	Incubation: 30°C, Assay: 25°C	0.1 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Acetonitrile	50%	100.0	43.0	%	Incubation: 100 mM GTA buffer, Assay: 100 mM GTA buffer, 4% Ethanol, 1% acetonitrile	Incubation: 9, Assay: 9	Incubation: 30°C, Assay: 25°C	0.1 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Isopropanol	50%	100.0	25.2	%	Incubation: 100 mM GTA buffer, Assay: 100 mM GTA buffer, 4% Ethanol, 1% acetonitrile	Incubation: 9, Assay: 9	Incubation: 30°C, Assay: 25°C	0.1 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Ethanol	50%	100.0	50.3	%	Incubation: 100 mM GTA buffer, Assay: 100 mM GTA buffer, 4% Ethanol, 1% acetonitrile	Incubation: 9, Assay: 9	Incubation: 30°C, Assay: 25°C	0.1 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Methanol	50%	100.0	43.4	%	Incubation: 100 mM GTA buffer, Assay: 100 mM GTA buffer, 4% Ethanol, 1% acetonitrile	Incubation: 9, Assay: 9	Incubation: 30°C, Assay: 25°C	0.1 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Dimethylformamide (DMF)	30%	100.0	87.4	%	Incubation: 100 mM GTA buffer, Assay: 100 mM GTA buffer, 4% Ethanol, 1% acetonitrile	Incubation: 9, Assay: 9	Incubation: 30°C, Assay: 25°C	0.1 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Acetone	30%	100.0	100.0	%	Incubation: 100 mM GTA buffer, Assay: 100 mM GTA buffer, 4% Ethanol, 1% acetonitrile	Incubation: 9, Assay: 9	Incubation: 30°C, Assay: 25°C	0.1 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	30%	100.0	74.4	%	Incubation: 100 mM GTA buffer, Assay: 100 mM GTA buffer, 4% Ethanol, 1% acetonitrile	Incubation: 9, Assay: 9	Incubation: 30°C, Assay: 25°C	0.1 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Acetonitrile	30%	100.0	101.1	%	Incubation: 100 mM GTA buffer, Assay: 100 mM GTA buffer, 4% Ethanol, 1% acetonitrile	Incubation: 9, Assay: 9	Incubation: 30°C, Assay: 25°C	0.1 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Isopropanol	30%	100.0	115.8	%	Incubation: 100 mM GTA buffer, Assay: 100 mM GTA buffer, 4% Ethanol, 1% acetonitrile	Incubation: 9, Assay: 9	Incubation: 30°C, Assay: 25°C	0.1 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Ethanol	30%	100.0	101.4	%	Incubation: 100 mM GTA buffer, Assay: 100 mM GTA buffer, 4% Ethanol, 1% acetonitrile	Incubation: 9, Assay: 9	Incubation: 30°C, Assay: 25°C	0.1 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Methanol	10%	100.0	95.0	%	Incubation: 100 mM GTA buffer, Assay: 100 mM GTA buffer, 4% Ethanol, 1% acetonitrile	Incubation: 9, Assay: 9	Incubation: 30°C, Assay: 25°C	0.1 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Dimethylformamide (DMF)	20%	100.0	96.8	%	Incubation: 100 mM GTA buffer, Assay: 100 mM GTA buffer, 4% Ethanol, 1% acetonitrile	Incubation: 9, Assay: 9	Incubation: 30°C, Assay: 25°C	0.1 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Acetone	20%	100.0	103.9	%	Incubation: 100 mM GTA buffer, Assay: 100 mM GTA buffer, 4% Ethanol, 1% acetonitrile	Incubation: 9, Assay: 9	Incubation: 30°C, Assay: 25°C	0.1 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	20%	100.0	86.0	%	Incubation: 100 mM GTA buffer, Assay: 100 mM GTA buffer, 4% Ethanol, 1% acetonitrile	Incubation: 9, Assay: 9	Incubation: 30°C, Assay: 25°C	0.1 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Acetonitrile	20%	100.0	110.5	%	Incubation: 100 mM GTA buffer, Assay: 100 mM GTA buffer, 4% Ethanol, 1% acetonitrile	Incubation: 9, Assay: 9	Incubation: 30°C, Assay: 25°C	0.1 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Isopropanol	20%	100.0	111.2	%	Incubation: 100 mM GTA buffer, Assay: 100 mM GTA buffer, 4% Ethanol, 1% acetonitrile	Incubation: 9, Assay: 9	Incubation: 30°C, Assay: 25°C	0.1 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Ethanol	20%	100.0	99.9	%	Incubation: 100 mM GTA buffer, Assay: 100 mM GTA buffer, 4% Ethanol, 1% acetonitrile	Incubation: 9, Assay: 9	Incubation: 30°C, Assay: 25°C	0.1 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Methanol	20%	100.0	92.7	%	Incubation: 100 mM GTA buffer, Assay: 100 mM GTA buffer, 4% Ethanol, 1% acetonitrile	Incubation: 9, Assay: 9	Incubation: 30°C, Assay: 25°C	0.1 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Dimethylformamide (DMF)	10%	100.0	91.6	%	Incubation: 100 mM GTA buffer, Assay: 100 mM GTA buffer, 4% Ethanol, 1% acetonitrile	Incubation: 9, Assay: 9	Incubation: 30°C, Assay: 25°C	0.1 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Acetone	10%	100.0	109.4	%	Incubation: 100 mM GTA buffer, Assay: 100 mM GTA buffer, 4% Ethanol, 1% acetonitrile	Incubation: 9, Assay: 9	Incubation: 30°C, Assay: 25°C	0.1 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	10%	100.0	91.8	%	Incubation: 100 mM GTA buffer, Assay: 100 mM GTA buffer, 4% Ethanol, 1% acetonitrile	Incubation: 9, Assay: 9	Incubation: 30°C, Assay: 25°C	0.1 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Acetonitrile	10%	100.0	95.1	%	Incubation: 100 mM GTA buffer, Assay: 100 mM GTA buffer, 4% Ethanol, 1% acetonitrile	Incubation: 9, Assay: 9	Incubation: 30°C, Assay: 25°C	0.1 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Isopropanol	10%	100.0	119.0	%	Incubation: 100 mM GTA buffer, Assay: 100 mM GTA buffer, 4% Ethanol, 1% acetonitrile	Incubation: 9, Assay: 9	Incubation: 30°C, Assay: 25°C	0.1 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Ethanol	10%	100.0	97.8	%	Incubation: 100 mM GTA buffer, Assay: 100 mM GTA buffer, 4% Ethanol, 1% acetonitrile	Incubation: 9, Assay: 9	Incubation: 30°C, Assay: 25°C	0.1 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase

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Visualization : Stability — Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

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