Esterase (gene estHIJ) from Bacillus halodurans NAH-Egypt
Enzyme Description
Extremophile
Yes
"alkalophilic", "halotolerant" organism
EC Number
Sequence
MKLVAPKPFTFEGGTRAVLLLHGFTGTTADVRMLGRYLQEKGYTCHAPLYKGHGVPPEELIQTGPDDWWEDVEAGYQHLKEQGYEEIAVCGLSLGGVFSLKIGYTLPVKGIVPMCAPMRPKTDDAIYKGVLEYAEEYKRREKKSDEQIEEEMQRFKSAPQTTLFGLKQLIEDVRDHLDHIYAPVFVVQARHDEMIDVESANVIHDTVESDEKSLKWYEDSTHVITLDKEKEQLHEDVYRFLEGLNWSE
Nehad Noby et al. (2020)
β "Recombinant cold -adapted halotolerant, organic solvent-stable esterase (estHIJ) from Bacillus halodurans
Analytical Biochemistry
Β· doi:10.1016/j.ab.2019.113554 β
Β· Stability - Incubation
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Database ID
UniProt: A0A2K9UV48 β
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)
Experimental Data (28 measurements)
28 measurements β page 1 of 2
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Stability - Incubation | Activity after incubation (15 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase | Methanol | 10% | 100.0 | 119.0 | % | Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer | Incubation: 8, Assay: 8 | Incubation: 25Β°C, Assay: 30Β°C | 0.5 mM p-Nitrophenol acetate | Acetic Acid , p-Nitrophenol | β | Yes, incubation "with agitation" | Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions |
| Stability - Incubation | Activity after incubation (15 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase | Toluene | 50% | 100.0 | 125.5 | % | Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer | Incubation: 8, Assay: 8 | Incubation: 25Β°C, Assay: 30Β°C | 0.5 mM p-Nitrophenol acetate | Acetic Acid , p-Nitrophenol | β | Yes, incubation "with agitation" | Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions |
| Stability - Incubation | Activity after incubation (15 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase | Toluene | 30% | 100.0 | 125.0 | % | Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer | Incubation: 8, Assay: 8 | Incubation: 25Β°C, Assay: 30Β°C | 0.5 mM p-Nitrophenol acetate | Acetic Acid , p-Nitrophenol | β | Yes, incubation "with agitation" | Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions |
| Stability - Incubation | Activity after incubation (15 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase | n-Hexane | 50% | 100.0 | 45.0 | % | Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer | Incubation: 8, Assay: 8 | Incubation: 25Β°C, Assay: 30Β°C | 0.5 mM p-Nitrophenol acetate | Acetic Acid , p-Nitrophenol | β | Yes, incubation "with agitation" | Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions |
| Stability - Incubation | Activity after incubation (15 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase | n-Hexane | 30% | 100.0 | 58.5 | % | Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer | Incubation: 8, Assay: 8 | Incubation: 25Β°C, Assay: 30Β°C | 0.5 mM p-Nitrophenol acetate | Acetic Acid , p-Nitrophenol | β | Yes, incubation "with agitation" | Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions |
| Stability - Incubation | Activity after incubation (15 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase | Petroleum Ether | 50% | 100.0 | 50.0 | % | Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer | Incubation: 8, Assay: 8 | Incubation: 25Β°C, Assay: 30Β°C | 0.5 mM p-Nitrophenol acetate | Acetic Acid , p-Nitrophenol | β | Yes, incubation "with agitation" | Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions |
| Stability - Incubation | Activity after incubation (15 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase | Petroleum Ether | 30% | 100.0 | 121.0 | % | Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer | Incubation: 8, Assay: 8 | Incubation: 25Β°C, Assay: 30Β°C | 0.5 mM p-Nitrophenol acetate | Acetic Acid , p-Nitrophenol | β | Yes, incubation "with agitation" | Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions |
| Stability - Incubation | Activity after incubation (15 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase | Isopropanol | 30% | 100.0 | 0.0 | % | Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer | Incubation: 8, Assay: 8 | Incubation: 25Β°C, Assay: 30Β°C | 0.5 mM p-Nitrophenol acetate | Acetic Acid , p-Nitrophenol | β | Yes, incubation "with agitation" | Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions |
| Stability - Incubation | Activity after incubation (15 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase | Isopropanol | 10% | 100.0 | 56.0 | % | Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer | Incubation: 8, Assay: 8 | Incubation: 25Β°C, Assay: 30Β°C | 0.5 mM p-Nitrophenol acetate | Acetic Acid , p-Nitrophenol | β | Yes, incubation "with agitation" | Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions |
| Stability - Incubation | Activity after incubation (15 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase | Acetone | 30% | 100.0 | 0.0 | % | Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer | Incubation: 8, Assay: 8 | Incubation: 25Β°C, Assay: 30Β°C | 0.5 mM p-Nitrophenol acetate | Acetic Acid , p-Nitrophenol | β | Yes, incubation "with agitation" | Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions |
| Stability - Incubation | Activity after incubation (15 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase | Acetone | 10% | 100.0 | 93.0 | % | Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer | Incubation: 8, Assay: 8 | Incubation: 25Β°C, Assay: 30Β°C | 0.5 mM p-Nitrophenol acetate | Acetic Acid , p-Nitrophenol | β | Yes, incubation "with agitation" | Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions |
| Stability - Incubation | Activity after incubation (15 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase | Ethanol | 30% | 100.0 | 0.0 | % | Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer | Incubation: 8, Assay: 8 | Incubation: 25Β°C, Assay: 30Β°C | 0.5 mM p-Nitrophenol acetate | Acetic Acid , p-Nitrophenol | β | Yes, incubation "with agitation" | Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions |
| Stability - Incubation | Activity after incubation (15 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase | Ethanol | 10% | 100.0 | 104.0 | % | Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer | Incubation: 8, Assay: 8 | Incubation: 25Β°C, Assay: 30Β°C | 0.5 mM p-Nitrophenol acetate | Acetic Acid , p-Nitrophenol | β | Yes, incubation "with agitation" | Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions |
| Stability - Incubation | Activity after incubation (15 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase | Methanol | 30% | 100.0 | 0.0 | % | Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer | Incubation: 8, Assay: 8 | Incubation: 25Β°C, Assay: 30Β°C | 0.5 mM p-Nitrophenol acetate | Acetic Acid , p-Nitrophenol | β | Yes, incubation "with agitation" | Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions |
| Stability - Incubation | Activity after incubation (3 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase | Methanol | 10% | 100.0 | 125.0 | % | Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer | Incubation: 8, Assay: 8 | Incubation: 25Β°C, Assay: 30Β°C | 0.5 mM p-Nitrophenol acetate | Acetic Acid , p-Nitrophenol | β | Yes, incubation "with agitation" | Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions |
| Stability - Incubation | Activity after incubation (3 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase | Toluene | 50% | 100.0 | 158.0 | % | Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer | Incubation: 8, Assay: 8 | Incubation: 25Β°C, Assay: 30Β°C | 0.5 mM p-Nitrophenol acetate | Acetic Acid , p-Nitrophenol | β | Yes, incubation "with agitation" | Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions |
| Stability - Incubation | Activity after incubation (3 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase | Toluene | 30% | 100.0 | 114.0 | % | Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer | Incubation: 8, Assay: 8 | Incubation: 25Β°C, Assay: 30Β°C | 0.5 mM p-Nitrophenol acetate | Acetic Acid , p-Nitrophenol | β | Yes, incubation "with agitation" | Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions |
| Stability - Incubation | Activity after incubation (3 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase | n-Hexane | 50% | 100.0 | 115.0 | % | Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer | Incubation: 8, Assay: 8 | Incubation: 25Β°C, Assay: 30Β°C | 0.5 mM p-Nitrophenol acetate | Acetic Acid , p-Nitrophenol | β | Yes, incubation "with agitation" | Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions |
| Stability - Incubation | Activity after incubation (3 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase | n-Hexane | 30% | 100.0 | 131.5 | % | Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer | Incubation: 8, Assay: 8 | Incubation: 25Β°C, Assay: 30Β°C | 0.5 mM p-Nitrophenol acetate | Acetic Acid , p-Nitrophenol | β | Yes, incubation "with agitation" | Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions |
| Stability - Incubation | Activity after incubation (3 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase | Petroleum Ether | 50% | 100.0 | 229.5 | % | Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer | Incubation: 8, Assay: 8 | Incubation: 25Β°C, Assay: 30Β°C | 0.5 mM p-Nitrophenol acetate | Acetic Acid , p-Nitrophenol | β | Yes, incubation "with agitation" | Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions |
Visualization : Stability β Incubation
One bar per measurement. Colour = solvent, shade = solvent volume.