Esterase (gene estHIJ) from Bacillus halodurans NAH-Egypt

Enzyme Description

Species
Bacillus halodurans NAH-Egypt β†—
Extremophile
Yes "alkalophilic", "halotolerant" organism
EC Number
3.1.1.1 β†—

Sequence

Length: 248 amino acids
MKLVAPKPFTFEGGTRAVLLLHGFTGTTADVRMLGRYLQEKGYTCHAPLYKGHGVPPEELIQTGPDDWWEDVEAGYQHLKEQGYEEIAVCGLSLGGVFSLKIGYTLPVKGIVPMCAPMRPKTDDAIYKGVLEYAEEYKRREKKSDEQIEEEMQRFKSAPQTTLFGLKQLIEDVRDHLDHIYAPVFVVQARHDEMIDVESANVIHDTVESDEKSLKWYEDSTHVITLDKEKEQLHEDVYRFLEGLNWSE
Nehad Noby et al. (2020) β€” "Recombinant cold -adapted halotolerant, organic solvent-stable esterase (estHIJ) from Bacillus halodurans
Analytical Biochemistry  Β· doi:10.1016/j.ab.2019.113554 β†—  Β· Stability - Incubation
28 measurements ▼
Database ID
UniProt: A0A2K9UV48 β†—
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (28 measurements)

28 measurements β€” page 2 of 2
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (3 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Petroleum Ether 30% 100.0 134.0 % Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer Incubation: 8, Assay: 8 Incubation: 25Β°C, Assay: 30Β°C 0.5 mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” Yes, incubation "with agitation" Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions
Stability - Incubation Activity after incubation (3 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Isopropanol 30% 100.0 10.0 % Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer Incubation: 8, Assay: 8 Incubation: 25Β°C, Assay: 30Β°C 0.5 mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” Yes, incubation "with agitation" Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions
Stability - Incubation Activity after incubation (3 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Isopropanol 10% 100.0 133.0 % Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer Incubation: 8, Assay: 8 Incubation: 25Β°C, Assay: 30Β°C 0.5 mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” Yes, incubation "with agitation" Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions
Stability - Incubation Activity after incubation (3 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Acetone 30% 100.0 0.0 % Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer Incubation: 8, Assay: 8 Incubation: 25Β°C, Assay: 30Β°C 0.5 mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” Yes, incubation "with agitation" Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions
Stability - Incubation Activity after incubation (3 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Acetone 10% 100.0 130.5 % Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer Incubation: 8, Assay: 8 Incubation: 25Β°C, Assay: 30Β°C 0.5 mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” Yes, incubation "with agitation" Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions
Stability - Incubation Activity after incubation (3 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Ethanol 30% 100.0 20.0 % Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer Incubation: 8, Assay: 8 Incubation: 25Β°C, Assay: 30Β°C 0.5 mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” Yes, incubation "with agitation" Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions
Stability - Incubation Activity after incubation (3 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Ethanol 10% 100.0 124.0 % Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer Incubation: 8, Assay: 8 Incubation: 25Β°C, Assay: 30Β°C 0.5 mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” Yes, incubation "with agitation" Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions
Stability - Incubation Activity after incubation (3 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Methanol 30% 100.0 70.0 % Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer Incubation: 8, Assay: 8 Incubation: 25Β°C, Assay: 30Β°C 0.5 mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” Yes, incubation "with agitation" Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions
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Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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