Pyruvate decarboxylase from Acetobacter pasteurianus

Enzyme Description

Species
Acetobacter pasteurianus β†—
Extremophile
No
EC Number
4.1.1.1 β†—

Sequence

Length: 558 amino acids
MTYTVGMYLAERLVQIGLKHHFAVAGDYNLVLLDQLLLNKDMKQIYCCNELNCGFSAEGYARSNGAAAAVVTFSVGAISAMNALGGAYAENLPVILISGAPNSNDQGTGHILHHTIGKTDYSYQLEMARQVTCAAESITDAHSAPAKIDHVIRTALRERKPAYLDIACNIASEPCVRPGPVSSLLSEPEIDHTSLKAAVDATVALLEKSASPVMLLGSKLRAANALAATETLADKLQCAVTIMAAAKGFFPEDHAGFRGLYWGEVSNPGVQELVETSDALLCIAPVFNDYSTVGWSAWPKGPNVILAEPDRVTVDGRAYDGFTLRAFLQALAEKAPARPASAQKSSVPTCSLTATSDEAGLTNDEIVRHINALLTSNTTLVAETGDSWFNAMRMTLPRGARVELEMQWGHIGWSVPSAFGNAMGSQDRQHVVMVGDGSFQLTAQEVAQMVRYELPVIIFLINNRGYVIEIAIHDGPYNYIKNWDYAGLMEVFNAGEGHGLGLKATTPKELTEAIARAKANTRGPTLIECQIDRTDCTDMLVQWGRKVASTNARKTTLA
Samuel Sutiono et al. (2019) β€” To beat the heat–engineering of the most thermostable pyruvate decarboxylase to date
RSC Advances  Β· doi:10.1039/c9ra06251c β†—  Β· Stability - Incubation
59 measurements ▼
Database ID
UniProt: A0A4Y3TS39 β†—
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (59 measurements)

59 measurements β€” page 1 of 3
Mutation Property Assay Solvent Solvent Volume Aqueous ReferenceWT Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
WT Stability - Incubation Activity after incubation (1 hour at 60Β°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase 1-Butanol 9% 100 β€” 2 % Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer Incubation: 6.5, Assay: 6.5 Incubation: 60Β°C, Assay: 25Β°C 25 mM Pyruvate Acetaldehyde , Carbon Dioxide (CO2) Incubation: 0.1 mM TDP, 5 mM MgSOβ‚„, Assay: 0.1 mM TDP, 5 mM MgSOβ‚„ β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions/Explicit protein sequence given corresponds to UniProt A0A4Y3TS39 which is annotated as for a protein from Acetobacter peroxydans
WT Stability - Incubation Activity after incubation (1 hour at 60Β°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase 1-Butanol 0.5% 100 β€” 94 % Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer Incubation: 6.5, Assay: 6.5 Incubation: 60Β°C, Assay: 25Β°C 25 mM Pyruvate Acetaldehyde , Carbon Dioxide (CO2) Incubation: 0.1 mM TDP, 5 mM MgSOβ‚„, Assay: 0.1 mM TDP, 5 mM MgSOβ‚„ β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions/Explicit protein sequence given corresponds to UniProt A0A4Y3TS39 which is annotated as for a protein from Acetobacter peroxydans
WT Stability - Incubation Activity after incubation (1 hour at 60Β°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase 1-Butanol 1% 100 β€” 81 % Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer Incubation: 6.5, Assay: 6.5 Incubation: 60Β°C, Assay: 25Β°C 25 mM Pyruvate Acetaldehyde , Carbon Dioxide (CO2) Incubation: 0.1 mM TDP, 5 mM MgSOβ‚„, Assay: 0.1 mM TDP, 5 mM MgSOβ‚„ β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions/Explicit protein sequence given corresponds to UniProt A0A4Y3TS39 which is annotated as for a protein from Acetobacter peroxydans
WT Stability - Incubation Activity after incubation (1 hour at 60Β°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase 1-Butanol 2% 100 β€” 53 % Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer Incubation: 6.5, Assay: 6.5 Incubation: 60Β°C, Assay: 25Β°C 25 mM Pyruvate Acetaldehyde , Carbon Dioxide (CO2) Incubation: 0.1 mM TDP, 5 mM MgSOβ‚„, Assay: 0.1 mM TDP, 5 mM MgSOβ‚„ β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions/Explicit protein sequence given corresponds to UniProt A0A4Y3TS39 which is annotated as for a protein from Acetobacter peroxydans
WT Stability - Incubation Activity after incubation (1 hour at 60Β°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase 1-Butanol 3% 100 β€” 14 % Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer Incubation: 6.5, Assay: 6.5 Incubation: 60Β°C, Assay: 25Β°C 25 mM Pyruvate Acetaldehyde , Carbon Dioxide (CO2) Incubation: 0.1 mM TDP, 5 mM MgSOβ‚„, Assay: 0.1 mM TDP, 5 mM MgSOβ‚„ β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions/Explicit protein sequence given corresponds to UniProt A0A4Y3TS39 which is annotated as for a protein from Acetobacter peroxydans
WT Stability - Incubation Activity after incubation (1 hour at 60Β°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase 1-Butanol 4% 100 β€” 7 % Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer Incubation: 6.5, Assay: 6.5 Incubation: 60Β°C, Assay: 25Β°C 25 mM Pyruvate Acetaldehyde , Carbon Dioxide (CO2) Incubation: 0.1 mM TDP, 5 mM MgSOβ‚„, Assay: 0.1 mM TDP, 5 mM MgSOβ‚„ β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions/Explicit protein sequence given corresponds to UniProt A0A4Y3TS39 which is annotated as for a protein from Acetobacter peroxydans
WT Stability - Incubation Activity after incubation (1 hour at 60Β°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase 1-Butanol 5% 100 β€” 4 % Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer Incubation: 6.5, Assay: 6.5 Incubation: 60Β°C, Assay: 25Β°C 25 mM Pyruvate Acetaldehyde , Carbon Dioxide (CO2) Incubation: 0.1 mM TDP, 5 mM MgSOβ‚„, Assay: 0.1 mM TDP, 5 mM MgSOβ‚„ β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions/Explicit protein sequence given corresponds to UniProt A0A4Y3TS39 which is annotated as for a protein from Acetobacter peroxydans
WT Stability - Incubation Activity after incubation (1 hour at 60Β°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase 1-Butanol 6% 100 β€” 3 % Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer Incubation: 6.5, Assay: 6.5 Incubation: 60Β°C, Assay: 25Β°C 25 mM Pyruvate Acetaldehyde , Carbon Dioxide (CO2) Incubation: 0.1 mM TDP, 5 mM MgSOβ‚„, Assay: 0.1 mM TDP, 5 mM MgSOβ‚„ β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions/Explicit protein sequence given corresponds to UniProt A0A4Y3TS39 which is annotated as for a protein from Acetobacter peroxydans
WT Stability - Incubation Activity after incubation (1 hour at 60Β°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase 1-Butanol 7% 100 β€” 4 % Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer Incubation: 6.5, Assay: 6.5 Incubation: 60Β°C, Assay: 25Β°C 25 mM Pyruvate Acetaldehyde , Carbon Dioxide (CO2) Incubation: 0.1 mM TDP, 5 mM MgSOβ‚„, Assay: 0.1 mM TDP, 5 mM MgSOβ‚„ β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions/Explicit protein sequence given corresponds to UniProt A0A4Y3TS39 which is annotated as for a protein from Acetobacter peroxydans
WT Stability - Incubation Activity after incubation (1 hour at 60Β°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase 1-Butanol 8% 100 β€” 4 % Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer Incubation: 6.5, Assay: 6.5 Incubation: 60Β°C, Assay: 25Β°C 25 mM Pyruvate Acetaldehyde , Carbon Dioxide (CO2) Incubation: 0.1 mM TDP, 5 mM MgSOβ‚„, Assay: 0.1 mM TDP, 5 mM MgSOβ‚„ β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions/Explicit protein sequence given corresponds to UniProt A0A4Y3TS39 which is annotated as for a protein from Acetobacter peroxydans
WT Stability - Incubation Activity after incubation (1 hour at 50Β°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase 1-Butanol 1% 100 β€” 98 % Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer Incubation: 6.5, Assay: 6.5 Incubation: 50Β°C, Assay: 25Β°C 25 mM Pyruvate Acetaldehyde , Carbon Dioxide (CO2) Incubation: 0.1 mM TDP, 5 mM MgSOβ‚„, Assay: 0.1 mM TDP, 5 mM MgSOβ‚„ β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions/Explicit protein sequence given corresponds to UniProt A0A4Y3TS39 which is annotated as for a protein from Acetobacter peroxydans
WT Stability - Incubation Activity after incubation (1 hour at 50Β°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase Ethanol 4% 100 β€” 99 % Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer Incubation: 6.5, Assay: 6.5 Incubation: 50Β°C, Assay: 25Β°C 25 mM Pyruvate Acetaldehyde , Carbon Dioxide (CO2) Incubation: 0.1 mM TDP, 5 mM MgSOβ‚„, Assay: 0.1 mM TDP, 5 mM MgSOβ‚„ β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions/Explicit protein sequence given corresponds to UniProt A0A4Y3TS39 which is annotated as for a protein from Acetobacter peroxydans
WT Stability - Incubation Activity after incubation (1 hour at 50Β°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase Ethanol 8% 100 β€” 98 % Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer Incubation: 6.5, Assay: 6.5 Incubation: 50Β°C, Assay: 25Β°C 25 mM Pyruvate Acetaldehyde , Carbon Dioxide (CO2) Incubation: 0.1 mM TDP, 5 mM MgSOβ‚„, Assay: 0.1 mM TDP, 5 mM MgSOβ‚„ β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions/Explicit protein sequence given corresponds to UniProt A0A4Y3TS39 which is annotated as for a protein from Acetobacter peroxydans
WT Stability - Incubation Activity after incubation (1 hour at 50Β°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase Ethanol 12% 100 β€” 97 % Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer Incubation: 6.5, Assay: 6.5 Incubation: 50Β°C, Assay: 25Β°C 25 mM Pyruvate Acetaldehyde , Carbon Dioxide (CO2) Incubation: 0.1 mM TDP, 5 mM MgSOβ‚„, Assay: 0.1 mM TDP, 5 mM MgSOβ‚„ β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions/Explicit protein sequence given corresponds to UniProt A0A4Y3TS39 which is annotated as for a protein from Acetobacter peroxydans
WT Stability - Incubation Activity after incubation (1 hour at 50Β°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase Ethanol 16% 100 β€” 94 % Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer Incubation: 6.5, Assay: 6.5 Incubation: 50Β°C, Assay: 25Β°C 25 mM Pyruvate Acetaldehyde , Carbon Dioxide (CO2) Incubation: 0.1 mM TDP, 5 mM MgSOβ‚„, Assay: 0.1 mM TDP, 5 mM MgSOβ‚„ β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions/Explicit protein sequence given corresponds to UniProt A0A4Y3TS39 which is annotated as for a protein from Acetobacter peroxydans
WT Stability - Incubation Activity after incubation (1 hour at 50Β°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase Ethanol 20% 100 β€” 74 % Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer Incubation: 6.5, Assay: 6.5 Incubation: 50Β°C, Assay: 25Β°C 25 mM Pyruvate Acetaldehyde , Carbon Dioxide (CO2) Incubation: 0.1 mM TDP, 5 mM MgSOβ‚„, Assay: 0.1 mM TDP, 5 mM MgSOβ‚„ β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions/Explicit protein sequence given corresponds to UniProt A0A4Y3TS39 which is annotated as for a protein from Acetobacter peroxydans
WT Stability - Incubation Activity after incubation (1 hour at 50Β°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase Ethanol 24% 100 β€” 32 % Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer Incubation: 6.5, Assay: 6.5 Incubation: 50Β°C, Assay: 25Β°C 25 mM Pyruvate Acetaldehyde , Carbon Dioxide (CO2) Incubation: 0.1 mM TDP, 5 mM MgSOβ‚„, Assay: 0.1 mM TDP, 5 mM MgSOβ‚„ β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions/Explicit protein sequence given corresponds to UniProt A0A4Y3TS39 which is annotated as for a protein from Acetobacter peroxydans
WT Stability - Incubation Activity after incubation (1 hour at 50Β°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase Ethanol 28% 100 β€” 3 % Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer Incubation: 6.5, Assay: 6.5 Incubation: 50Β°C, Assay: 25Β°C 25 mM Pyruvate Acetaldehyde , Carbon Dioxide (CO2) Incubation: 0.1 mM TDP, 5 mM MgSOβ‚„, Assay: 0.1 mM TDP, 5 mM MgSOβ‚„ β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions/Explicit protein sequence given corresponds to UniProt A0A4Y3TS39 which is annotated as for a protein from Acetobacter peroxydans
WT Stability - Incubation Activity after incubation (1 hour at 50Β°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase Ethanol 32% 100 β€” 3 % Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer Incubation: 6.5, Assay: 6.5 Incubation: 50Β°C, Assay: 25Β°C 25 mM Pyruvate Acetaldehyde , Carbon Dioxide (CO2) Incubation: 0.1 mM TDP, 5 mM MgSOβ‚„, Assay: 0.1 mM TDP, 5 mM MgSOβ‚„ β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions/Explicit protein sequence given corresponds to UniProt A0A4Y3TS39 which is annotated as for a protein from Acetobacter peroxydans
WT Stability - Incubation Activity after incubation (1 hour at 50Β°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase 1-Butanol 9% 100 β€” 5 % Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer Incubation: 6.5, Assay: 6.5 Incubation: 50Β°C, Assay: 25Β°C 25 mM Pyruvate Acetaldehyde , Carbon Dioxide (CO2) Incubation: 0.1 mM TDP, 5 mM MgSOβ‚„, Assay: 0.1 mM TDP, 5 mM MgSOβ‚„ β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions/Explicit protein sequence given corresponds to UniProt A0A4Y3TS39 which is annotated as for a protein from Acetobacter peroxydans
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Mutations in this dataset (2)

WT L84I/A100S/S103T/Q106Y/K118T/S122N/A128V/R129K/Q130H/T139V/D140S/H142E/S143E/L441V

Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume. β€” β€” β€” Reference value. Hover for details.

Mutation Effect

Mutation impact on enzyme stability and function in the presence of organic solvent: comparison of wild-type and mutant values in identical conditions.

Structure

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