Pyruvate decarboxylase from Acetobacter pasteurianus

Enzyme Description

Species

Extremophile

EC Number

4.1.1.1 ↗

Sequence

Length: 558 amino acids

MTYTVGMYLAERLVQIGLKHHFAVAGDYNLVLLDQLLLNKDMKQIYCCNELNCGFSAEGYARSNGAAAAVVTFSVGAISAMNALGGAYAENLPVILISGAPNSNDQGTGHILHHTIGKTDYSYQLEMARQVTCAAESITDAHSAPAKIDHVIRTALRERKPAYLDIACNIASEPCVRPGPVSSLLSEPEIDHTSLKAAVDATVALLEKSASPVMLLGSKLRAANALAATETLADKLQCAVTIMAAAKGFFPEDHAGFRGLYWGEVSNPGVQELVETSDALLCIAPVFNDYSTVGWSAWPKGPNVILAEPDRVTVDGRAYDGFTLRAFLQALAEKAPARPASAQKSSVPTCSLTATSDEAGLTNDEIVRHINALLTSNTTLVAETGDSWFNAMRMTLPRGARVELEMQWGHIGWSVPSAFGNAMGSQDRQHVVMVGDGSFQLTAQEVAQMVRYELPVIIFLINNRGYVIEIAIHDGPYNYIKNWDYAGLMEVFNAGEGHGLGLKATTPKELTEAIARAKANTRGPTLIECQIDRTDCTDMLVQWGRKVASTNARKTTLA

Samuel Sutiono et al. (2019) — To beat the heat–engineering of the most thermostable pyruvate decarboxylase to date

RSC Advances · doi:10.1039/c9ra06251c ↗ · Stability - Incubation

59 measurements ▼

Database ID

UniProt: A0A4Y3TS39 ↗

Sequence Annotation

Explicit - Provided

Protein Source

Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (59 measurements)

59 measurements — page 3 of 3

Mutation	Property	Assay	Solvent	Solvent Volume	Aqueous Reference	WT Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
L84I/A100S/S103T/Q106Y/K118T/S122N/A128V/R129K/Q130H/T139V/D140S/H142E/S143E/L441V	Stability - Incubation	Activity after incubation (1 hour at 60°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase	1-Butanol	0.5%	100	94	99	%	Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer	Incubation: 6.5, Assay: 6.5	Incubation: 60°C, Assay: 25°C	25 mM Pyruvate	Acetaldehyde , Carbon Dioxide (CO2)	Incubation: 0.1 mM TDP, 5 mM MgSO₄, Assay: 0.1 mM TDP, 5 mM MgSO₄	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous conditions/Explicit protein sequence given corresponds to UniProt A0A4Y3TS39 which is annotated as for a protein from Acetobacter peroxydans
L84I/A100S/S103T/Q106Y/K118T/S122N/A128V/R129K/Q130H/T139V/D140S/H142E/S143E/L441V	Stability - Incubation	Activity after incubation (1 hour at 50°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase	1-Butanol	8%	100	3	94	%	Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer	Incubation: 6.5, Assay: 6.5	Incubation: 50°C, Assay: 25°C	25 mM Pyruvate	Acetaldehyde , Carbon Dioxide (CO2)	Incubation: 0.1 mM TDP, 5 mM MgSO₄, Assay: 0.1 mM TDP, 5 mM MgSO₄	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous conditions/Explicit protein sequence given corresponds to UniProt A0A4Y3TS39 which is annotated as for a protein from Acetobacter peroxydans
L84I/A100S/S103T/Q106Y/K118T/S122N/A128V/R129K/Q130H/T139V/D140S/H142E/S143E/L441V	Stability - Incubation	Activity after incubation (1 hour at 50°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase	1-Butanol	7%	100	3	97	%	Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer	Incubation: 6.5, Assay: 6.5	Incubation: 50°C, Assay: 25°C	25 mM Pyruvate	Acetaldehyde , Carbon Dioxide (CO2)	Incubation: 0.1 mM TDP, 5 mM MgSO₄, Assay: 0.1 mM TDP, 5 mM MgSO₄	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous conditions/Explicit protein sequence given corresponds to UniProt A0A4Y3TS39 which is annotated as for a protein from Acetobacter peroxydans
L84I/A100S/S103T/Q106Y/K118T/S122N/A128V/R129K/Q130H/T139V/D140S/H142E/S143E/L441V	Stability - Incubation	Activity after incubation (1 hour at 50°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase	1-Butanol	6%	100	22	102	%	Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer	Incubation: 6.5, Assay: 6.5	Incubation: 50°C, Assay: 25°C	25 mM Pyruvate	Acetaldehyde , Carbon Dioxide (CO2)	Incubation: 0.1 mM TDP, 5 mM MgSO₄, Assay: 0.1 mM TDP, 5 mM MgSO₄	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous conditions/Explicit protein sequence given corresponds to UniProt A0A4Y3TS39 which is annotated as for a protein from Acetobacter peroxydans
L84I/A100S/S103T/Q106Y/K118T/S122N/A128V/R129K/Q130H/T139V/D140S/H142E/S143E/L441V	Stability - Incubation	Activity after incubation (1 hour at 50°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase	1-Butanol	5%	100	70	100	%	Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer	Incubation: 6.5, Assay: 6.5	Incubation: 50°C, Assay: 25°C	25 mM Pyruvate	Acetaldehyde , Carbon Dioxide (CO2)	Incubation: 0.1 mM TDP, 5 mM MgSO₄, Assay: 0.1 mM TDP, 5 mM MgSO₄	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous conditions/Explicit protein sequence given corresponds to UniProt A0A4Y3TS39 which is annotated as for a protein from Acetobacter peroxydans
L84I/A100S/S103T/Q106Y/K118T/S122N/A128V/R129K/Q130H/T139V/D140S/H142E/S143E/L441V	Stability - Incubation	Activity after incubation (1 hour at 50°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase	1-Butanol	4%	100	86	104	%	Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer	Incubation: 6.5, Assay: 6.5	Incubation: 50°C, Assay: 25°C	25 mM Pyruvate	Acetaldehyde , Carbon Dioxide (CO2)	Incubation: 0.1 mM TDP, 5 mM MgSO₄, Assay: 0.1 mM TDP, 5 mM MgSO₄	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous conditions/Explicit protein sequence given corresponds to UniProt A0A4Y3TS39 which is annotated as for a protein from Acetobacter peroxydans
L84I/A100S/S103T/Q106Y/K118T/S122N/A128V/R129K/Q130H/T139V/D140S/H142E/S143E/L441V	Stability - Incubation	Activity after incubation (1 hour at 50°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase	1-Butanol	3%	100	95	103	%	Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer	Incubation: 6.5, Assay: 6.5	Incubation: 50°C, Assay: 25°C	25 mM Pyruvate	Acetaldehyde , Carbon Dioxide (CO2)	Incubation: 0.1 mM TDP, 5 mM MgSO₄, Assay: 0.1 mM TDP, 5 mM MgSO₄	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous conditions/Explicit protein sequence given corresponds to UniProt A0A4Y3TS39 which is annotated as for a protein from Acetobacter peroxydans
L84I/A100S/S103T/Q106Y/K118T/S122N/A128V/R129K/Q130H/T139V/D140S/H142E/S143E/L441V	Stability - Incubation	Activity after incubation (1 hour at 50°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase	1-Butanol	2%	100	94	102	%	Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer	Incubation: 6.5, Assay: 6.5	Incubation: 50°C, Assay: 25°C	25 mM Pyruvate	Acetaldehyde , Carbon Dioxide (CO2)	Incubation: 0.1 mM TDP, 5 mM MgSO₄, Assay: 0.1 mM TDP, 5 mM MgSO₄	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous conditions/Explicit protein sequence given corresponds to UniProt A0A4Y3TS39 which is annotated as for a protein from Acetobacter peroxydans
L84I/A100S/S103T/Q106Y/K118T/S122N/A128V/R129K/Q130H/T139V/D140S/H142E/S143E/L441V	Stability - Incubation	Activity after incubation (1 hour at 50°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase	1-Butanol	1%	100	98	101	%	Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer	Incubation: 6.5, Assay: 6.5	Incubation: 50°C, Assay: 25°C	25 mM Pyruvate	Acetaldehyde , Carbon Dioxide (CO2)	Incubation: 0.1 mM TDP, 5 mM MgSO₄, Assay: 0.1 mM TDP, 5 mM MgSO₄	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous conditions/Explicit protein sequence given corresponds to UniProt A0A4Y3TS39 which is annotated as for a protein from Acetobacter peroxydans
L84I/A100S/S103T/Q106Y/K118T/S122N/A128V/R129K/Q130H/T139V/D140S/H142E/S143E/L441V	Stability - Incubation	Activity after incubation (1 hour at 50°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase	1-Butanol	0.5%	100	99	102	%	Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer	Incubation: 6.5, Assay: 6.5	Incubation: 50°C, Assay: 25°C	25 mM Pyruvate	Acetaldehyde , Carbon Dioxide (CO2)	Incubation: 0.1 mM TDP, 5 mM MgSO₄, Assay: 0.1 mM TDP, 5 mM MgSO₄	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous conditions/Explicit protein sequence given corresponds to UniProt A0A4Y3TS39 which is annotated as for a protein from Acetobacter peroxydans
L84I/A100S/S103T/Q106Y/K118T/S122N/A128V/R129K/Q130H/T139V/D140S/H142E/S143E/L441V	Stability - Incubation	Activity after incubation (1 hour at 50°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase	1-Butanol	9%	100	5	72	%	Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer	Incubation: 6.5, Assay: 6.5	Incubation: 50°C, Assay: 25°C	25 mM Pyruvate	Acetaldehyde , Carbon Dioxide (CO2)	Incubation: 0.1 mM TDP, 5 mM MgSO₄, Assay: 0.1 mM TDP, 5 mM MgSO₄	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous conditions/Explicit protein sequence given corresponds to UniProt A0A4Y3TS39 which is annotated as for a protein from Acetobacter peroxydans
L84I/A100S/S103T/Q106Y/K118T/S122N/A128V/R129K/Q130H/T139V/D140S/H142E/S143E/L441V	Stability - Incubation	Activity after incubation (1 hour at 60°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase	1-Butanol	1%	100	81	100	%	Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer	Incubation: 6.5, Assay: 6.5	Incubation: 60°C, Assay: 25°C	25 mM Pyruvate	Acetaldehyde , Carbon Dioxide (CO2)	Incubation: 0.1 mM TDP, 5 mM MgSO₄, Assay: 0.1 mM TDP, 5 mM MgSO₄	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous conditions/Explicit protein sequence given corresponds to UniProt A0A4Y3TS39 which is annotated as for a protein from Acetobacter peroxydans
L84I/A100S/S103T/Q106Y/K118T/S122N/A128V/R129K/Q130H/T139V/D140S/H142E/S143E/L441V	Stability - Incubation	Activity after incubation (1 hour at 60°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase	1-Butanol	2%	100	53	102	%	Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer	Incubation: 6.5, Assay: 6.5	Incubation: 60°C, Assay: 25°C	25 mM Pyruvate	Acetaldehyde , Carbon Dioxide (CO2)	Incubation: 0.1 mM TDP, 5 mM MgSO₄, Assay: 0.1 mM TDP, 5 mM MgSO₄	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous conditions/Explicit protein sequence given corresponds to UniProt A0A4Y3TS39 which is annotated as for a protein from Acetobacter peroxydans
L84I/A100S/S103T/Q106Y/K118T/S122N/A128V/R129K/Q130H/T139V/D140S/H142E/S143E/L441V	Stability - Incubation	Activity after incubation (1 hour at 60°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase	1-Butanol	3%	100	14	101	%	Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer	Incubation: 6.5, Assay: 6.5	Incubation: 60°C, Assay: 25°C	25 mM Pyruvate	Acetaldehyde , Carbon Dioxide (CO2)	Incubation: 0.1 mM TDP, 5 mM MgSO₄, Assay: 0.1 mM TDP, 5 mM MgSO₄	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous conditions/Explicit protein sequence given corresponds to UniProt A0A4Y3TS39 which is annotated as for a protein from Acetobacter peroxydans
L84I/A100S/S103T/Q106Y/K118T/S122N/A128V/R129K/Q130H/T139V/D140S/H142E/S143E/L441V	Stability - Incubation	Activity after incubation (1 hour at 60°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase	1-Butanol	4%	100	7	102	%	Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer	Incubation: 6.5, Assay: 6.5	Incubation: 60°C, Assay: 25°C	25 mM Pyruvate	Acetaldehyde , Carbon Dioxide (CO2)	Incubation: 0.1 mM TDP, 5 mM MgSO₄, Assay: 0.1 mM TDP, 5 mM MgSO₄	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous conditions/Explicit protein sequence given corresponds to UniProt A0A4Y3TS39 which is annotated as for a protein from Acetobacter peroxydans
L84I/A100S/S103T/Q106Y/K118T/S122N/A128V/R129K/Q130H/T139V/D140S/H142E/S143E/L441V	Stability - Incubation	Activity after incubation (1 hour at 60°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase	1-Butanol	5%	100	4	101	%	Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer	Incubation: 6.5, Assay: 6.5	Incubation: 60°C, Assay: 25°C	25 mM Pyruvate	Acetaldehyde , Carbon Dioxide (CO2)	Incubation: 0.1 mM TDP, 5 mM MgSO₄, Assay: 0.1 mM TDP, 5 mM MgSO₄	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous conditions/Explicit protein sequence given corresponds to UniProt A0A4Y3TS39 which is annotated as for a protein from Acetobacter peroxydans
L84I/A100S/S103T/Q106Y/K118T/S122N/A128V/R129K/Q130H/T139V/D140S/H142E/S143E/L441V	Stability - Incubation	Activity after incubation (1 hour at 60°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase	1-Butanol	6%	100	3	97	%	Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer	Incubation: 6.5, Assay: 6.5	Incubation: 60°C, Assay: 25°C	25 mM Pyruvate	Acetaldehyde , Carbon Dioxide (CO2)	Incubation: 0.1 mM TDP, 5 mM MgSO₄, Assay: 0.1 mM TDP, 5 mM MgSO₄	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous conditions/Explicit protein sequence given corresponds to UniProt A0A4Y3TS39 which is annotated as for a protein from Acetobacter peroxydans
L84I/A100S/S103T/Q106Y/K118T/S122N/A128V/R129K/Q130H/T139V/D140S/H142E/S143E/L441V	Stability - Incubation	Activity after incubation (1 hour at 60°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase	1-Butanol	7%	100	4	88	%	Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer	Incubation: 6.5, Assay: 6.5	Incubation: 60°C, Assay: 25°C	25 mM Pyruvate	Acetaldehyde , Carbon Dioxide (CO2)	Incubation: 0.1 mM TDP, 5 mM MgSO₄, Assay: 0.1 mM TDP, 5 mM MgSO₄	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous conditions/Explicit protein sequence given corresponds to UniProt A0A4Y3TS39 which is annotated as for a protein from Acetobacter peroxydans
L84I/A100S/S103T/Q106Y/K118T/S122N/A128V/R129K/Q130H/T139V/D140S/H142E/S143E/L441V	Stability - Incubation	Activity after incubation (1 hour at 60°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase	1-Butanol	8%	100	4	46	%	Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer	Incubation: 6.5, Assay: 6.5	Incubation: 60°C, Assay: 25°C	25 mM Pyruvate	Acetaldehyde , Carbon Dioxide (CO2)	Incubation: 0.1 mM TDP, 5 mM MgSO₄, Assay: 0.1 mM TDP, 5 mM MgSO₄	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous conditions/Explicit protein sequence given corresponds to UniProt A0A4Y3TS39 which is annotated as for a protein from Acetobacter peroxydans

Stability - Incubation

Activity after incubation (1 hour at 60°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase

1-Butanol

100

—

Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer

Incubation: 6.5, Assay: 6.5

Incubation: 60°C, Assay: 25°C

25 mM Pyruvate

Acetaldehyde, Carbon Dioxide (CO2)

Incubation: 0.1 mM TDP, 5 mM MgSO₄, Assay: 0.1 mM TDP, 5 mM MgSO₄

—

Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions/Explicit protein sequence given corresponds to UniProt A0A4Y3TS39 which is annotated as for a protein from Acetobacter peroxydans

Stability - Incubation

Activity after incubation (1 hour at 60°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase

1-Butanol

0.5%

100

—

Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer

Incubation: 6.5, Assay: 6.5

Incubation: 60°C, Assay: 25°C

25 mM Pyruvate

Acetaldehyde, Carbon Dioxide (CO2)

Incubation: 0.1 mM TDP, 5 mM MgSO₄, Assay: 0.1 mM TDP, 5 mM MgSO₄

—

Stability - Incubation

Activity after incubation (1 hour at 60°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase

1-Butanol

100

—

Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer

Incubation: 6.5, Assay: 6.5

Incubation: 60°C, Assay: 25°C

25 mM Pyruvate

Acetaldehyde, Carbon Dioxide (CO2)

Incubation: 0.1 mM TDP, 5 mM MgSO₄, Assay: 0.1 mM TDP, 5 mM MgSO₄

—

Stability - Incubation

Activity after incubation (1 hour at 60°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase

1-Butanol

100

—

Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer

Incubation: 6.5, Assay: 6.5

Incubation: 60°C, Assay: 25°C

25 mM Pyruvate

Acetaldehyde, Carbon Dioxide (CO2)

Incubation: 0.1 mM TDP, 5 mM MgSO₄, Assay: 0.1 mM TDP, 5 mM MgSO₄

—

Stability - Incubation

Activity after incubation (1 hour at 60°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase

1-Butanol

100

—

Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer

Incubation: 6.5, Assay: 6.5

Incubation: 60°C, Assay: 25°C

25 mM Pyruvate

Acetaldehyde, Carbon Dioxide (CO2)

Incubation: 0.1 mM TDP, 5 mM MgSO₄, Assay: 0.1 mM TDP, 5 mM MgSO₄

—

Stability - Incubation

Activity after incubation (1 hour at 60°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase

1-Butanol

100

—

Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer

Incubation: 6.5, Assay: 6.5

Incubation: 60°C, Assay: 25°C

25 mM Pyruvate

Acetaldehyde, Carbon Dioxide (CO2)

Incubation: 0.1 mM TDP, 5 mM MgSO₄, Assay: 0.1 mM TDP, 5 mM MgSO₄

—

Stability - Incubation

Activity after incubation (1 hour at 60°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase

1-Butanol

100

—

Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer

Incubation: 6.5, Assay: 6.5

Incubation: 60°C, Assay: 25°C

25 mM Pyruvate

Acetaldehyde, Carbon Dioxide (CO2)

Incubation: 0.1 mM TDP, 5 mM MgSO₄, Assay: 0.1 mM TDP, 5 mM MgSO₄

—

Stability - Incubation

Activity after incubation (1 hour at 60°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase

1-Butanol

100

—

Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer

Incubation: 6.5, Assay: 6.5

Incubation: 60°C, Assay: 25°C

25 mM Pyruvate

Acetaldehyde, Carbon Dioxide (CO2)

Incubation: 0.1 mM TDP, 5 mM MgSO₄, Assay: 0.1 mM TDP, 5 mM MgSO₄

—

Stability - Incubation

Activity after incubation (1 hour at 60°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase

1-Butanol

100

—

Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer

Incubation: 6.5, Assay: 6.5

Incubation: 60°C, Assay: 25°C

25 mM Pyruvate

Acetaldehyde, Carbon Dioxide (CO2)

Incubation: 0.1 mM TDP, 5 mM MgSO₄, Assay: 0.1 mM TDP, 5 mM MgSO₄

—

Stability - Incubation

Activity after incubation (1 hour at 60°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase

1-Butanol

100

—

Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer

Incubation: 6.5, Assay: 6.5

Incubation: 60°C, Assay: 25°C

25 mM Pyruvate

Acetaldehyde, Carbon Dioxide (CO2)

Incubation: 0.1 mM TDP, 5 mM MgSO₄, Assay: 0.1 mM TDP, 5 mM MgSO₄

—

Stability - Incubation

Activity after incubation (1 hour at 50°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase

1-Butanol

100

—

Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer

Incubation: 6.5, Assay: 6.5

Incubation: 50°C, Assay: 25°C

25 mM Pyruvate

Acetaldehyde, Carbon Dioxide (CO2)

Incubation: 0.1 mM TDP, 5 mM MgSO₄, Assay: 0.1 mM TDP, 5 mM MgSO₄

—

Stability - Incubation

Activity after incubation (1 hour at 50°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase

Ethanol

100

—

Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer

Incubation: 6.5, Assay: 6.5

Incubation: 50°C, Assay: 25°C

25 mM Pyruvate

Acetaldehyde, Carbon Dioxide (CO2)

Incubation: 0.1 mM TDP, 5 mM MgSO₄, Assay: 0.1 mM TDP, 5 mM MgSO₄

—

Stability - Incubation

Activity after incubation (1 hour at 50°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase

Ethanol

100

—

Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer

Incubation: 6.5, Assay: 6.5

Incubation: 50°C, Assay: 25°C

25 mM Pyruvate

Acetaldehyde, Carbon Dioxide (CO2)

Incubation: 0.1 mM TDP, 5 mM MgSO₄, Assay: 0.1 mM TDP, 5 mM MgSO₄

—

Stability - Incubation

Activity after incubation (1 hour at 50°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase

Ethanol

12%

100

—

Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer

Incubation: 6.5, Assay: 6.5

Incubation: 50°C, Assay: 25°C

25 mM Pyruvate

Acetaldehyde, Carbon Dioxide (CO2)

Incubation: 0.1 mM TDP, 5 mM MgSO₄, Assay: 0.1 mM TDP, 5 mM MgSO₄

—

Stability - Incubation

Activity after incubation (1 hour at 50°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase

Ethanol

16%

100

—

Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer

Incubation: 6.5, Assay: 6.5

Incubation: 50°C, Assay: 25°C

25 mM Pyruvate

Acetaldehyde, Carbon Dioxide (CO2)

Incubation: 0.1 mM TDP, 5 mM MgSO₄, Assay: 0.1 mM TDP, 5 mM MgSO₄

—

Stability - Incubation

Activity after incubation (1 hour at 50°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase

Ethanol

20%

100

—

Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer

Incubation: 6.5, Assay: 6.5

Incubation: 50°C, Assay: 25°C

25 mM Pyruvate

Acetaldehyde, Carbon Dioxide (CO2)

Incubation: 0.1 mM TDP, 5 mM MgSO₄, Assay: 0.1 mM TDP, 5 mM MgSO₄

—

Stability - Incubation

Activity after incubation (1 hour at 50°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase

Ethanol

24%

100

—

Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer

Incubation: 6.5, Assay: 6.5

Incubation: 50°C, Assay: 25°C

25 mM Pyruvate

Acetaldehyde, Carbon Dioxide (CO2)

Incubation: 0.1 mM TDP, 5 mM MgSO₄, Assay: 0.1 mM TDP, 5 mM MgSO₄

—

Stability - Incubation

Activity after incubation (1 hour at 50°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase

Ethanol

28%

100

—

Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer

Incubation: 6.5, Assay: 6.5

Incubation: 50°C, Assay: 25°C

25 mM Pyruvate

Acetaldehyde, Carbon Dioxide (CO2)

Incubation: 0.1 mM TDP, 5 mM MgSO₄, Assay: 0.1 mM TDP, 5 mM MgSO₄

—

Stability - Incubation

Activity after incubation (1 hour at 50°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase

Ethanol

32%

100

—

Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer

Incubation: 6.5, Assay: 6.5

Incubation: 50°C, Assay: 25°C

25 mM Pyruvate

Acetaldehyde, Carbon Dioxide (CO2)

Incubation: 0.1 mM TDP, 5 mM MgSO₄, Assay: 0.1 mM TDP, 5 mM MgSO₄

—

Stability - Incubation

Activity after incubation (1 hour at 50°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase

1-Butanol

100

—

Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer

Incubation: 6.5, Assay: 6.5

Incubation: 50°C, Assay: 25°C

25 mM Pyruvate

Acetaldehyde, Carbon Dioxide (CO2)

Incubation: 0.1 mM TDP, 5 mM MgSO₄, Assay: 0.1 mM TDP, 5 mM MgSO₄

—

Stability - Incubation

Activity after incubation (1 hour at 50°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase

1-Butanol

100

—

Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer

Incubation: 6.5, Assay: 6.5

Incubation: 50°C, Assay: 25°C

25 mM Pyruvate

Acetaldehyde, Carbon Dioxide (CO2)

Incubation: 0.1 mM TDP, 5 mM MgSO₄, Assay: 0.1 mM TDP, 5 mM MgSO₄

—

Stability - Incubation

Activity after incubation (1 hour at 50°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase

1-Butanol

100

—

Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer

Incubation: 6.5, Assay: 6.5

Incubation: 50°C, Assay: 25°C

25 mM Pyruvate

Acetaldehyde, Carbon Dioxide (CO2)

Incubation: 0.1 mM TDP, 5 mM MgSO₄, Assay: 0.1 mM TDP, 5 mM MgSO₄

—

Stability - Incubation

Activity after incubation (1 hour at 50°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase

1-Butanol

100

—

Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer

Incubation: 6.5, Assay: 6.5

Incubation: 50°C, Assay: 25°C

25 mM Pyruvate

Acetaldehyde, Carbon Dioxide (CO2)

Incubation: 0.1 mM TDP, 5 mM MgSO₄, Assay: 0.1 mM TDP, 5 mM MgSO₄

—

Stability - Incubation

Activity after incubation (1 hour at 50°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase

1-Butanol

100

—

Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer

Incubation: 6.5, Assay: 6.5

Incubation: 50°C, Assay: 25°C

25 mM Pyruvate

Acetaldehyde, Carbon Dioxide (CO2)

Incubation: 0.1 mM TDP, 5 mM MgSO₄, Assay: 0.1 mM TDP, 5 mM MgSO₄

—

Stability - Incubation

Activity after incubation (1 hour at 50°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase

1-Butanol

100

—

Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer

Incubation: 6.5, Assay: 6.5

Incubation: 50°C, Assay: 25°C

25 mM Pyruvate

Acetaldehyde, Carbon Dioxide (CO2)

Incubation: 0.1 mM TDP, 5 mM MgSO₄, Assay: 0.1 mM TDP, 5 mM MgSO₄

—

Stability - Incubation

Activity after incubation (1 hour at 50°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase

1-Butanol

100

—

Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer

Incubation: 6.5, Assay: 6.5

Incubation: 50°C, Assay: 25°C

25 mM Pyruvate

Acetaldehyde, Carbon Dioxide (CO2)

Incubation: 0.1 mM TDP, 5 mM MgSO₄, Assay: 0.1 mM TDP, 5 mM MgSO₄

—

Stability - Incubation

Activity after incubation (1 hour at 50°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase

1-Butanol

100

—

Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer

Incubation: 6.5, Assay: 6.5

Incubation: 50°C, Assay: 25°C

25 mM Pyruvate

Acetaldehyde, Carbon Dioxide (CO2)

Incubation: 0.1 mM TDP, 5 mM MgSO₄, Assay: 0.1 mM TDP, 5 mM MgSO₄

—

Stability - Incubation

Activity after incubation (1 hour at 50°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase

1-Butanol

0.5%

100

—

Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer

Incubation: 6.5, Assay: 6.5

Incubation: 50°C, Assay: 25°C

25 mM Pyruvate

Acetaldehyde, Carbon Dioxide (CO2)

Incubation: 0.1 mM TDP, 5 mM MgSO₄, Assay: 0.1 mM TDP, 5 mM MgSO₄

—

L84I/A100S/S103T/Q106Y/K118T/S122N/A128V/R129K/Q130H/T139V/D140S/H142E/S143E/L441V

Stability - Incubation

Activity after incubation (1 hour at 50°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase

Ethanol

15%

100

—

107

Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer

Incubation: 6.5, Assay: 6.5

Incubation: 50°C, Assay: 25°C

25 mM Pyruvate

Acetaldehyde, Carbon Dioxide (CO2)

Incubation: 0.1 mM TDP, 5 mM MgSO₄, Assay: 0.1 mM TDP, 5 mM MgSO₄

—

L84I/A100S/S103T/Q106Y/K118T/S122N/A128V/R129K/Q130H/T139V/D140S/H142E/S143E/L441V

Stability - Incubation

Activity after incubation (1 hour at 60°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase

1-Butanol

100

Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer

Incubation: 6.5, Assay: 6.5

Incubation: 60°C, Assay: 25°C

25 mM Pyruvate

Acetaldehyde, Carbon Dioxide (CO2)

Incubation: 0.1 mM TDP, 5 mM MgSO₄, Assay: 0.1 mM TDP, 5 mM MgSO₄

—

L84I/A100S/S103T/Q106Y/K118T/S122N/A128V/R129K/Q130H/T139V/D140S/H142E/S143E/L441V

Stability - Incubation

Activity after incubation (1 hour at 50°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase

Ethanol

10%

100

—

105

Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer

Incubation: 6.5, Assay: 6.5

Incubation: 50°C, Assay: 25°C

25 mM Pyruvate

Acetaldehyde, Carbon Dioxide (CO2)

Incubation: 0.1 mM TDP, 5 mM MgSO₄, Assay: 0.1 mM TDP, 5 mM MgSO₄

—

L84I/A100S/S103T/Q106Y/K118T/S122N/A128V/R129K/Q130H/T139V/D140S/H142E/S143E/L441V

Stability - Incubation

Activity after incubation (1 hour at 50°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase

Ethanol

100

—

102

Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer

Incubation: 6.5, Assay: 6.5

Incubation: 50°C, Assay: 25°C

25 mM Pyruvate

Acetaldehyde, Carbon Dioxide (CO2)

Incubation: 0.1 mM TDP, 5 mM MgSO₄, Assay: 0.1 mM TDP, 5 mM MgSO₄

—

L84I/A100S/S103T/Q106Y/K118T/S122N/A128V/R129K/Q130H/T139V/D140S/H142E/S143E/L441V

Stability - Incubation

Activity after incubation (1 hour at 50°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase

Ethanol

18%

100

—

109

Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer

Incubation: 6.5, Assay: 6.5

Incubation: 50°C, Assay: 25°C

25 mM Pyruvate

Acetaldehyde, Carbon Dioxide (CO2)

Incubation: 0.1 mM TDP, 5 mM MgSO₄, Assay: 0.1 mM TDP, 5 mM MgSO₄

—

L84I/A100S/S103T/Q106Y/K118T/S122N/A128V/R129K/Q130H/T139V/D140S/H142E/S143E/L441V

Stability - Incubation

Activity after incubation (1 hour at 50°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase

Ethanol

20%

100

109

Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer

Incubation: 6.5, Assay: 6.5

Incubation: 50°C, Assay: 25°C

25 mM Pyruvate

Acetaldehyde, Carbon Dioxide (CO2)

Incubation: 0.1 mM TDP, 5 mM MgSO₄, Assay: 0.1 mM TDP, 5 mM MgSO₄

—

L84I/A100S/S103T/Q106Y/K118T/S122N/A128V/R129K/Q130H/T139V/D140S/H142E/S143E/L441V

Stability - Incubation

Activity after incubation (1 hour at 50°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase

Ethanol

25%

100

—

108

Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer

Incubation: 6.5, Assay: 6.5

Incubation: 50°C, Assay: 25°C

25 mM Pyruvate

Acetaldehyde, Carbon Dioxide (CO2)

Incubation: 0.1 mM TDP, 5 mM MgSO₄, Assay: 0.1 mM TDP, 5 mM MgSO₄

—

L84I/A100S/S103T/Q106Y/K118T/S122N/A128V/R129K/Q130H/T139V/D140S/H142E/S143E/L441V

Stability - Incubation

Activity after incubation (1 hour at 50°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase

Ethanol

30%

100

—

103

Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer

Incubation: 6.5, Assay: 6.5

Incubation: 50°C, Assay: 25°C

25 mM Pyruvate

Acetaldehyde, Carbon Dioxide (CO2)

Incubation: 0.1 mM TDP, 5 mM MgSO₄, Assay: 0.1 mM TDP, 5 mM MgSO₄

—

L84I/A100S/S103T/Q106Y/K118T/S122N/A128V/R129K/Q130H/T139V/D140S/H142E/S143E/L441V

Stability - Incubation

Activity after incubation (1 hour at 50°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase

Ethanol

35%

100

—

Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer

Incubation: 6.5, Assay: 6.5

Incubation: 50°C, Assay: 25°C

25 mM Pyruvate

Acetaldehyde, Carbon Dioxide (CO2)

Incubation: 0.1 mM TDP, 5 mM MgSO₄, Assay: 0.1 mM TDP, 5 mM MgSO₄

—

L84I/A100S/S103T/Q106Y/K118T/S122N/A128V/R129K/Q130H/T139V/D140S/H142E/S143E/L441V

Stability - Incubation

Activity after incubation (1 hour at 50°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase

Ethanol

40%

100

—

Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer

Incubation: 6.5, Assay: 6.5

Incubation: 50°C, Assay: 25°C

25 mM Pyruvate

Acetaldehyde, Carbon Dioxide (CO2)

Incubation: 0.1 mM TDP, 5 mM MgSO₄, Assay: 0.1 mM TDP, 5 mM MgSO₄

—

L84I/A100S/S103T/Q106Y/K118T/S122N/A128V/R129K/Q130H/T139V/D140S/H142E/S143E/L441V

Stability - Incubation

Activity after incubation (1 hour at 50°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase

Ethanol

45%

100

—

Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer

Incubation: 6.5, Assay: 6.5

Incubation: 50°C, Assay: 25°C

25 mM Pyruvate

Acetaldehyde, Carbon Dioxide (CO2)

Incubation: 0.1 mM TDP, 5 mM MgSO₄, Assay: 0.1 mM TDP, 5 mM MgSO₄

—

L84I/A100S/S103T/Q106Y/K118T/S122N/A128V/R129K/Q130H/T139V/D140S/H142E/S143E/L441V

Stability - Incubation

Activity after incubation (1 hour at 50°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase

Ethanol

50%

100

—

Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer

Incubation: 6.5, Assay: 6.5

Incubation: 50°C, Assay: 25°C

25 mM Pyruvate

Acetaldehyde, Carbon Dioxide (CO2)

Incubation: 0.1 mM TDP, 5 mM MgSO₄, Assay: 0.1 mM TDP, 5 mM MgSO₄

—

L84I/A100S/S103T/Q106Y/K118T/S122N/A128V/R129K/Q130H/T139V/D140S/H142E/S143E/L441V

Stability - Incubation

Activity after incubation (1 hour at 60°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase

1-Butanol

0.5%

100

Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer

Incubation: 6.5, Assay: 6.5

Incubation: 60°C, Assay: 25°C

25 mM Pyruvate

Acetaldehyde, Carbon Dioxide (CO2)

Incubation: 0.1 mM TDP, 5 mM MgSO₄, Assay: 0.1 mM TDP, 5 mM MgSO₄

—

L84I/A100S/S103T/Q106Y/K118T/S122N/A128V/R129K/Q130H/T139V/D140S/H142E/S143E/L441V

Stability - Incubation

Activity after incubation (1 hour at 50°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase

1-Butanol

100

Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer

Incubation: 6.5, Assay: 6.5

Incubation: 50°C, Assay: 25°C

25 mM Pyruvate

Acetaldehyde, Carbon Dioxide (CO2)

Incubation: 0.1 mM TDP, 5 mM MgSO₄, Assay: 0.1 mM TDP, 5 mM MgSO₄

—

L84I/A100S/S103T/Q106Y/K118T/S122N/A128V/R129K/Q130H/T139V/D140S/H142E/S143E/L441V

Stability - Incubation

Activity after incubation (1 hour at 50°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase

1-Butanol

100

Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer

Incubation: 6.5, Assay: 6.5

Incubation: 50°C, Assay: 25°C

25 mM Pyruvate

Acetaldehyde, Carbon Dioxide (CO2)

Incubation: 0.1 mM TDP, 5 mM MgSO₄, Assay: 0.1 mM TDP, 5 mM MgSO₄

—

L84I/A100S/S103T/Q106Y/K118T/S122N/A128V/R129K/Q130H/T139V/D140S/H142E/S143E/L441V

Stability - Incubation

Activity after incubation (1 hour at 50°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase

1-Butanol

100

102

Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer

Incubation: 6.5, Assay: 6.5

Incubation: 50°C, Assay: 25°C

25 mM Pyruvate

Acetaldehyde, Carbon Dioxide (CO2)

Incubation: 0.1 mM TDP, 5 mM MgSO₄, Assay: 0.1 mM TDP, 5 mM MgSO₄

—

L84I/A100S/S103T/Q106Y/K118T/S122N/A128V/R129K/Q130H/T139V/D140S/H142E/S143E/L441V

Stability - Incubation

Activity after incubation (1 hour at 50°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase

1-Butanol

100

Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer

Incubation: 6.5, Assay: 6.5

Incubation: 50°C, Assay: 25°C

25 mM Pyruvate

Acetaldehyde, Carbon Dioxide (CO2)

Incubation: 0.1 mM TDP, 5 mM MgSO₄, Assay: 0.1 mM TDP, 5 mM MgSO₄

—

L84I/A100S/S103T/Q106Y/K118T/S122N/A128V/R129K/Q130H/T139V/D140S/H142E/S143E/L441V

Stability - Incubation

Activity after incubation (1 hour at 50°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase

1-Butanol

100

104

Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer

Incubation: 6.5, Assay: 6.5

Incubation: 50°C, Assay: 25°C

25 mM Pyruvate

Acetaldehyde, Carbon Dioxide (CO2)

Incubation: 0.1 mM TDP, 5 mM MgSO₄, Assay: 0.1 mM TDP, 5 mM MgSO₄

—

L84I/A100S/S103T/Q106Y/K118T/S122N/A128V/R129K/Q130H/T139V/D140S/H142E/S143E/L441V

Stability - Incubation

Activity after incubation (1 hour at 50°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase

1-Butanol

100

103

Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer

Incubation: 6.5, Assay: 6.5

Incubation: 50°C, Assay: 25°C

25 mM Pyruvate

Acetaldehyde, Carbon Dioxide (CO2)

Incubation: 0.1 mM TDP, 5 mM MgSO₄, Assay: 0.1 mM TDP, 5 mM MgSO₄

—

L84I/A100S/S103T/Q106Y/K118T/S122N/A128V/R129K/Q130H/T139V/D140S/H142E/S143E/L441V

Stability - Incubation

Activity after incubation (1 hour at 50°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase

1-Butanol

100

102

Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer

Incubation: 6.5, Assay: 6.5

Incubation: 50°C, Assay: 25°C

25 mM Pyruvate

Acetaldehyde, Carbon Dioxide (CO2)

Incubation: 0.1 mM TDP, 5 mM MgSO₄, Assay: 0.1 mM TDP, 5 mM MgSO₄

—

L84I/A100S/S103T/Q106Y/K118T/S122N/A128V/R129K/Q130H/T139V/D140S/H142E/S143E/L441V

Stability - Incubation

Activity after incubation (1 hour at 50°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase

1-Butanol

100

101

Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer

Incubation: 6.5, Assay: 6.5

Incubation: 50°C, Assay: 25°C

25 mM Pyruvate

Acetaldehyde, Carbon Dioxide (CO2)

Incubation: 0.1 mM TDP, 5 mM MgSO₄, Assay: 0.1 mM TDP, 5 mM MgSO₄

—

L84I/A100S/S103T/Q106Y/K118T/S122N/A128V/R129K/Q130H/T139V/D140S/H142E/S143E/L441V

Stability - Incubation

Activity after incubation (1 hour at 50°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase

1-Butanol

0.5%

100

102

Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer

Incubation: 6.5, Assay: 6.5

Incubation: 50°C, Assay: 25°C

25 mM Pyruvate

Acetaldehyde, Carbon Dioxide (CO2)

Incubation: 0.1 mM TDP, 5 mM MgSO₄, Assay: 0.1 mM TDP, 5 mM MgSO₄

—

L84I/A100S/S103T/Q106Y/K118T/S122N/A128V/R129K/Q130H/T139V/D140S/H142E/S143E/L441V

Stability - Incubation

Activity after incubation (1 hour at 50°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase

1-Butanol

100

Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer

Incubation: 6.5, Assay: 6.5

Incubation: 50°C, Assay: 25°C

25 mM Pyruvate

Acetaldehyde, Carbon Dioxide (CO2)

Incubation: 0.1 mM TDP, 5 mM MgSO₄, Assay: 0.1 mM TDP, 5 mM MgSO₄

—

L84I/A100S/S103T/Q106Y/K118T/S122N/A128V/R129K/Q130H/T139V/D140S/H142E/S143E/L441V

Stability - Incubation

Activity after incubation (1 hour at 60°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase

1-Butanol

100

Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer

Incubation: 6.5, Assay: 6.5

Incubation: 60°C, Assay: 25°C

25 mM Pyruvate

Acetaldehyde, Carbon Dioxide (CO2)

Incubation: 0.1 mM TDP, 5 mM MgSO₄, Assay: 0.1 mM TDP, 5 mM MgSO₄

—

L84I/A100S/S103T/Q106Y/K118T/S122N/A128V/R129K/Q130H/T139V/D140S/H142E/S143E/L441V

Stability - Incubation

Activity after incubation (1 hour at 60°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase

1-Butanol

100

102

Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer

Incubation: 6.5, Assay: 6.5

Incubation: 60°C, Assay: 25°C

25 mM Pyruvate

Acetaldehyde, Carbon Dioxide (CO2)

Incubation: 0.1 mM TDP, 5 mM MgSO₄, Assay: 0.1 mM TDP, 5 mM MgSO₄

—

L84I/A100S/S103T/Q106Y/K118T/S122N/A128V/R129K/Q130H/T139V/D140S/H142E/S143E/L441V

Stability - Incubation

Activity after incubation (1 hour at 60°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase

1-Butanol

100

101

Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer

Incubation: 6.5, Assay: 6.5

Incubation: 60°C, Assay: 25°C

25 mM Pyruvate

Acetaldehyde, Carbon Dioxide (CO2)

Incubation: 0.1 mM TDP, 5 mM MgSO₄, Assay: 0.1 mM TDP, 5 mM MgSO₄

—

L84I/A100S/S103T/Q106Y/K118T/S122N/A128V/R129K/Q130H/T139V/D140S/H142E/S143E/L441V

Stability - Incubation

Activity after incubation (1 hour at 60°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase

1-Butanol

100

102

Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer

Incubation: 6.5, Assay: 6.5

Incubation: 60°C, Assay: 25°C

25 mM Pyruvate

Acetaldehyde, Carbon Dioxide (CO2)

Incubation: 0.1 mM TDP, 5 mM MgSO₄, Assay: 0.1 mM TDP, 5 mM MgSO₄

—

L84I/A100S/S103T/Q106Y/K118T/S122N/A128V/R129K/Q130H/T139V/D140S/H142E/S143E/L441V

Stability - Incubation

Activity after incubation (1 hour at 60°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase

1-Butanol

100

101

Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer

Incubation: 6.5, Assay: 6.5

Incubation: 60°C, Assay: 25°C

25 mM Pyruvate

Acetaldehyde, Carbon Dioxide (CO2)

Incubation: 0.1 mM TDP, 5 mM MgSO₄, Assay: 0.1 mM TDP, 5 mM MgSO₄

—

L84I/A100S/S103T/Q106Y/K118T/S122N/A128V/R129K/Q130H/T139V/D140S/H142E/S143E/L441V

Stability - Incubation

Activity after incubation (1 hour at 60°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase

1-Butanol

100

Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer

Incubation: 6.5, Assay: 6.5

Incubation: 60°C, Assay: 25°C

25 mM Pyruvate

Acetaldehyde, Carbon Dioxide (CO2)

Incubation: 0.1 mM TDP, 5 mM MgSO₄, Assay: 0.1 mM TDP, 5 mM MgSO₄

—

L84I/A100S/S103T/Q106Y/K118T/S122N/A128V/R129K/Q130H/T139V/D140S/H142E/S143E/L441V

Stability - Incubation

Activity after incubation (1 hour at 60°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase

1-Butanol

100

Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer

Incubation: 6.5, Assay: 6.5

Incubation: 60°C, Assay: 25°C

25 mM Pyruvate

Acetaldehyde, Carbon Dioxide (CO2)

Incubation: 0.1 mM TDP, 5 mM MgSO₄, Assay: 0.1 mM TDP, 5 mM MgSO₄

—

L84I/A100S/S103T/Q106Y/K118T/S122N/A128V/R129K/Q130H/T139V/D140S/H142E/S143E/L441V

Stability - Incubation

Activity after incubation (1 hour at 60°C), measured by absorbance spectrophotometry (change in pyruvate absorbance measurement, 315 nm) in aqueous phase

1-Butanol

100

Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer

Incubation: 6.5, Assay: 6.5

Incubation: 60°C, Assay: 25°C

25 mM Pyruvate

Acetaldehyde, Carbon Dioxide (CO2)

Incubation: 0.1 mM TDP, 5 mM MgSO₄, Assay: 0.1 mM TDP, 5 mM MgSO₄

—

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Mutations in this dataset (2)

WT L84I/A100S/S103T/Q106Y/K118T/S122N/A128V/R129K/Q130H/T139V/D140S/H142E/S143E/L441V

Visualization : Stability — Incubation

One bar per measurement. Colour = solvent, shade = solvent volume. — — — Reference value. Hover for details.

Mutation Effect

Mutation impact on enzyme stability and function in the presence of organic solvent: comparison of wild-type and mutant values in identical conditions.

Structure

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Pyruvate decarboxylase from Acetobacter pasteurianus

Enzyme Description

Sequence

Experimental Data (59 measurements)

Visualization : Stability — Incubation

Mutation Effect

Structure

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