Chitinase (gene mtch509) from Microbulbifer thermotolerans DAU221
Enzyme Description
Extremophile
No
but "thermotolerant" organism
EC Number
Sequence
MKMFWLGLTALGLCAANAAAVDCRSLPDWENAGVYVGGDQVRHLNNAYTAKWWTQAEDPATHSGTWDVWRYDGACDGVSSSSSSSSSSSSSSSSGSGSSSGSSSSGGSCSSPQYVDGGRYSTGDLVQNVGSEFRCDVGGWCSLGGAYAPGVGWAWGEAWTQLGDCEGSSSSSSSSSGSSGSSGNPGSSGGNSGLLPKHALVGYWHNFNNGSGLYRIAEVSDVWDVIVVAFADDAGNGNVAFNLDAGLNKQQFIDDIAAKRAAGKIVVLSFGGQNGTVTLNSAEHVTNFVNSTADILDEYGFDGIDIDLESGAGVMHGAPVVQNLVSAVKQLKARYPNMYLSMAPEHPYVQGGYVAYTGIWGAYLPMIDQLRDELDLLHVQLYNNGGLSTPYHSQAYAAGSVDMMVASAMMLIEGFPLGGGSAGYFDGLRPDQVALGLPSGPSAAGSGQAATADINRALDCLTARVNCGSLTPSQAWPDFNGVMTWSINWDMHDGGIFSGPVGSHVHNLP
Hyo-Jung Lee et al. (2018)
β Cloning, purification, and characterization of an organic solvent-tolerant chitinase, MtCh509, from Microbulbifer thermotolerans DAU221
Biotechnology for Biofuels
Β· doi:10.1186/s13068-018-1299-1 β
Β· Activity - Classical
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Database ID
UniProt: A0A143HNF3 β
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)
Experimental Data (22 measurements)
22 measurements β page 1 of 2
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Activity - Classical | Activity measured by absorbance spectrophotometry (DNS method, 540 nm) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 20% | 100 | 158 | % | 50 mM citrate buffer | 5 | 55Β°C | 1% Colloidal Chitin | Reducing sugars | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (DNS method, 540 nm) in the presence of organic solvent | n-Hexane | 20% | 100 | 173 | % | 50 mM citrate buffer | 5 | 55Β°C | 1% Colloidal Chitin | Reducing sugars | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (DNS method, 540 nm) in the presence of organic solvent | Toluene | 20% | 100 | 157 | % | 50 mM citrate buffer | 5 | 55Β°C | 1% Colloidal Chitin | Reducing sugars | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (DNS method, 540 nm) in the presence of organic solvent | Benzene | 20% | 100 | 201 | % | 50 mM citrate buffer | 5 | 55Β°C | 1% Colloidal Chitin | Reducing sugars | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (DNS method, 540 nm) in the presence of organic solvent | Isoamyl Alcohol | 20% | 100 | 103 | % | 50 mM citrate buffer | 5 | 55Β°C | 1% Colloidal Chitin | Reducing sugars | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (DNS method, 540 nm) in the presence of organic solvent | 1-Butanol | 20% | 100 | 77 | % | 50 mM citrate buffer | 5 | 55Β°C | 1% Colloidal Chitin | Reducing sugars | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (DNS method, 540 nm) in the presence of organic solvent | Isopropanol | 20% | 100 | 16 | % | 50 mM citrate buffer | 5 | 55Β°C | 1% Colloidal Chitin | Reducing sugars | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (DNS method, 540 nm) in the presence of organic solvent | Acetonitrile | 20% | 100 | 6 | % | 50 mM citrate buffer | 5 | 55Β°C | 1% Colloidal Chitin | Reducing sugars | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (DNS method, 540 nm) in the presence of organic solvent | Ethanol | 20% | 100 | 19 | % | 50 mM citrate buffer | 5 | 55Β°C | 1% Colloidal Chitin | Reducing sugars | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (DNS method, 540 nm) in the presence of organic solvent | Acetone | 20% | 100 | 9 | % | 50 mM citrate buffer | 5 | 55Β°C | 1% Colloidal Chitin | Reducing sugars | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (DNS method, 540 nm) in the presence of organic solvent | Methanol | 20% | 100 | 9 | % | 50 mM citrate buffer | 5 | 55Β°C | 1% Colloidal Chitin | Reducing sugars | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (DNS method, 540 nm) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 10% | 100 | 139 | % | 50 mM citrate buffer | 5 | 55Β°C | 1% Colloidal Chitin | Reducing sugars | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (DNS method, 540 nm) in the presence of organic solvent | n-Hexane | 10% | 100 | 139 | % | 50 mM citrate buffer | 5 | 55Β°C | 1% Colloidal Chitin | Reducing sugars | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (DNS method, 540 nm) in the presence of organic solvent | Toluene | 10% | 100 | 165 | % | 50 mM citrate buffer | 5 | 55Β°C | 1% Colloidal Chitin | Reducing sugars | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (DNS method, 540 nm) in the presence of organic solvent | Benzene | 10% | 100 | 148 | % | 50 mM citrate buffer | 5 | 55Β°C | 1% Colloidal Chitin | Reducing sugars | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (DNS method, 540 nm) in the presence of organic solvent | Isoamyl Alcohol | 10% | 100 | 127 | % | 50 mM citrate buffer | 5 | 55Β°C | 1% Colloidal Chitin | Reducing sugars | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (DNS method, 540 nm) in the presence of organic solvent | 1-Butanol | 10% | 100 | 74 | % | 50 mM citrate buffer | 5 | 55Β°C | 1% Colloidal Chitin | Reducing sugars | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (DNS method, 540 nm) in the presence of organic solvent | Isopropanol | 10% | 100 | 33 | % | 50 mM citrate buffer | 5 | 55Β°C | 1% Colloidal Chitin | Reducing sugars | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (DNS method, 540 nm) in the presence of organic solvent | Acetonitrile | 10% | 100 | 20 | % | 50 mM citrate buffer | 5 | 55Β°C | 1% Colloidal Chitin | Reducing sugars | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (DNS method, 540 nm) in the presence of organic solvent | Ethanol | 10% | 100 | 44 | % | 50 mM citrate buffer | 5 | 55Β°C | 1% Colloidal Chitin | Reducing sugars | β | β | Classical aqueous control (in %) |
Visualization : Activity β Classical
One bar per measurement. Colour = solvent, shade = solvent volume.