Chitinase (gene mtch509) from Microbulbifer thermotolerans DAU221

Enzyme Description

Species

Microbulbifer thermotolerans DAU221 ↗

Extremophile

No but "thermotolerant" organism

EC Number

3.2.1.14 ↗

Sequence

Length: 509 amino acids

MKMFWLGLTALGLCAANAAAVDCRSLPDWENAGVYVGGDQVRHLNNAYTAKWWTQAEDPATHSGTWDVWRYDGACDGVSSSSSSSSSSSSSSSSGSGSSSGSSSSGGSCSSPQYVDGGRYSTGDLVQNVGSEFRCDVGGWCSLGGAYAPGVGWAWGEAWTQLGDCEGSSSSSSSSSGSSGSSGNPGSSGGNSGLLPKHALVGYWHNFNNGSGLYRIAEVSDVWDVIVVAFADDAGNGNVAFNLDAGLNKQQFIDDIAAKRAAGKIVVLSFGGQNGTVTLNSAEHVTNFVNSTADILDEYGFDGIDIDLESGAGVMHGAPVVQNLVSAVKQLKARYPNMYLSMAPEHPYVQGGYVAYTGIWGAYLPMIDQLRDELDLLHVQLYNNGGLSTPYHSQAYAAGSVDMMVASAMMLIEGFPLGGGSAGYFDGLRPDQVALGLPSGPSAAGSGQAATADINRALDCLTARVNCGSLTPSQAWPDFNGVMTWSINWDMHDGGIFSGPVGSHVHNLP

Hyo-Jung Lee et al. (2018) — Cloning, purification, and characterization of an organic solvent-tolerant chitinase, MtCh509, from Microbulbifer thermotolerans DAU221

Biotechnology for Biofuels · doi:10.1186/s13068-018-1299-1 ↗ · Activity - Classical

22 measurements ▼

Database ID

UniProt: A0A143HNF3 ↗

Sequence Annotation

Explicit - Provided

Protein Source

Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (22 measurements)

22 measurements — page 2 of 2

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS method, 540 nm) in the presence of organic solvent	Acetone	10%	100	31	%	50 mM citrate buffer	5	55°C	1% Colloidal Chitin	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS method, 540 nm) in the presence of organic solvent	Methanol	10%	100	84	%	50 mM citrate buffer	5	55°C	1% Colloidal Chitin	Reducing sugars	—	—	Classical aqueous control (in %)

Activity - Classical	Activity measured by absorbance spectrophotometry (DNS method, 540 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	20%	100	158	%	50 mM citrate buffer	5	55°C	1% Colloidal Chitin	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS method, 540 nm) in the presence of organic solvent	n-Hexane	20%	100	173	%	50 mM citrate buffer	5	55°C	1% Colloidal Chitin	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS method, 540 nm) in the presence of organic solvent	Toluene	20%	100	157	%	50 mM citrate buffer	5	55°C	1% Colloidal Chitin	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS method, 540 nm) in the presence of organic solvent	Benzene	20%	100	201	%	50 mM citrate buffer	5	55°C	1% Colloidal Chitin	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS method, 540 nm) in the presence of organic solvent	Isoamyl Alcohol	20%	100	103	%	50 mM citrate buffer	5	55°C	1% Colloidal Chitin	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS method, 540 nm) in the presence of organic solvent	1-Butanol	20%	100	77	%	50 mM citrate buffer	5	55°C	1% Colloidal Chitin	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS method, 540 nm) in the presence of organic solvent	Isopropanol	20%	100	16	%	50 mM citrate buffer	5	55°C	1% Colloidal Chitin	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS method, 540 nm) in the presence of organic solvent	Acetonitrile	20%	100	6	%	50 mM citrate buffer	5	55°C	1% Colloidal Chitin	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS method, 540 nm) in the presence of organic solvent	Ethanol	20%	100	19	%	50 mM citrate buffer	5	55°C	1% Colloidal Chitin	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS method, 540 nm) in the presence of organic solvent	Acetone	20%	100	9	%	50 mM citrate buffer	5	55°C	1% Colloidal Chitin	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS method, 540 nm) in the presence of organic solvent	Methanol	20%	100	9	%	50 mM citrate buffer	5	55°C	1% Colloidal Chitin	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS method, 540 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10%	100	139	%	50 mM citrate buffer	5	55°C	1% Colloidal Chitin	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS method, 540 nm) in the presence of organic solvent	n-Hexane	10%	100	139	%	50 mM citrate buffer	5	55°C	1% Colloidal Chitin	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS method, 540 nm) in the presence of organic solvent	Toluene	10%	100	165	%	50 mM citrate buffer	5	55°C	1% Colloidal Chitin	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS method, 540 nm) in the presence of organic solvent	Benzene	10%	100	148	%	50 mM citrate buffer	5	55°C	1% Colloidal Chitin	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS method, 540 nm) in the presence of organic solvent	Isoamyl Alcohol	10%	100	127	%	50 mM citrate buffer	5	55°C	1% Colloidal Chitin	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS method, 540 nm) in the presence of organic solvent	1-Butanol	10%	100	74	%	50 mM citrate buffer	5	55°C	1% Colloidal Chitin	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS method, 540 nm) in the presence of organic solvent	Isopropanol	10%	100	33	%	50 mM citrate buffer	5	55°C	1% Colloidal Chitin	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS method, 540 nm) in the presence of organic solvent	Acetonitrile	10%	100	20	%	50 mM citrate buffer	5	55°C	1% Colloidal Chitin	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS method, 540 nm) in the presence of organic solvent	Ethanol	10%	100	44	%	50 mM citrate buffer	5	55°C	1% Colloidal Chitin	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS method, 540 nm) in the presence of organic solvent	Acetone	10%	100	31	%	50 mM citrate buffer	5	55°C	1% Colloidal Chitin	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS method, 540 nm) in the presence of organic solvent	Methanol	10%	100	84	%	50 mM citrate buffer	5	55°C	1% Colloidal Chitin	Reducing sugars	—	—	Classical aqueous control (in %)

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Visualization : Activity — Classical

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