9-cis-epoxycarotenoid dioxygenase (gene SeNCED) from Serratia sp. ATCC 39006

Enzyme Description

Species
Serratia sp. ATCC 39006 β†—
Extremophile
No
EC Number
1.13.11.51 β†—

Sequence

Length: 496 amino acids
MSLKFPETPEFTGLYKPSRVETQVFDLEIEGEVPPQIKGTFFQVSPDSYYPPMQGKDIFFNGDGLVSAFKFENGHVSLRRRYVQTDRLKAQWKERRSLNGIYRNIYTNDPLAAENNTTANTTVLFHAGVLLAMKEDALPYALNPNTLETLGVWDFNGQITSATFTAHPKIDPDNGDLLCFAYEAKGDGTSDIAYFEIDASGKLKKEIWFKGPYAAMIHDFAVTAHHVVFPLIPLTADVDRMKAGGQHFEWQPDLPQLFGVLPRDGSSEDVLWFHGPKDGFQGHTLNAYEEDGLLRVDMPVTNGNVFYFFPQADGSVPLPETLSSQLMRWTFNLNGPGESEAGQQTLQPQPLTSFPCEFPRCDERFTGKPYEHGFVLAFDPALPFDETLGERPFQFFNQLAHVNVRTGETETWYPGNAQCFQEPIFVPRSPDAPEGDGYVIALLNHLHGNDTELVVLDSLKMATGPVARIKVPFRLRMSLHGNWTPAEALKGTQSGR
Jiao Tang et al. (2018) β€” Expression and characterization of a 9-cis-epoxycarotenoid dioxygenase from Serratia sp. ATCC 39006 capable of biotransforming isoeugenol and 4-vinylguaiacol to vanillin
Biotechnology Reports  Β· doi:10.1016/j.btre.2018.e00253 β†—  Β· Activity - Classical Stability - Incubation
60 measurements ▼
Database ID
UniProt: A0A2I5TAF0 β†—
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (60 measurements)

60 measurements β€” page 1 of 3
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity - Classical Activity measured by HPLC (vanillin production measurement) in the presence of organic solvent Chloroform 10% 100.0 176.16 % 100 mM potassium phosphate buffer, 10% v/v glycerol 8 Room temperature 2 mM Isoeugenol Acetaldehyde , Vanillin Fe²⁺ Yes, "Vigorous shaking" Classical aqueous control (in %)
Activity - Classical Activity measured by HPLC (vanillin production measurement) in the presence of organic solvent Dichloromethane 10% 100.0 93.83 % 100 mM potassium phosphate buffer, 10% v/v glycerol 8 Room temperature 2 mM Isoeugenol Acetaldehyde , Vanillin Fe²⁺ Yes, "Vigorous shaking" Classical aqueous control (in %)
Activity - Classical Activity measured by HPLC (vanillin production measurement) in the presence of organic solvent n-Octane 10% 100.0 70.45 % 100 mM potassium phosphate buffer, 10% v/v glycerol 8 Room temperature 2 mM 4-Vinylguaiacol Acetaldehyde , Vanillin Fe²⁺ Yes, "Vigorous shaking" Classical aqueous control (in %)
Activity - Classical Activity measured by HPLC (vanillin production measurement) in the presence of organic solvent 1-Butanol 10% 100.0 21.44 % 100 mM potassium phosphate buffer, 10% v/v glycerol 8 Room temperature 2 mM 4-Vinylguaiacol Acetaldehyde , Vanillin Fe²⁺ Yes, "Vigorous shaking" Classical aqueous control (in %)
Activity - Classical Activity measured by HPLC (vanillin production measurement) in the presence of organic solvent Petroleum Ether 10% 100.0 92.95 % 100 mM potassium phosphate buffer, 10% v/v glycerol 8 Room temperature 2 mM 4-Vinylguaiacol Acetaldehyde , Vanillin Fe²⁺ Yes, "Vigorous shaking" Classical aqueous control (in %)
Activity - Classical Activity measured by HPLC (vanillin production measurement) in the presence of organic solvent n-Hexane 10% 100.0 86.15 % 100 mM potassium phosphate buffer, 10% v/v glycerol 8 Room temperature 2 mM 4-Vinylguaiacol Acetaldehyde , Vanillin Fe²⁺ Yes, "Vigorous shaking" Classical aqueous control (in %)
Activity - Classical Activity measured by HPLC (vanillin production measurement) in the presence of organic solvent Cyclohexane 10% 100.0 122.72 % 100 mM potassium phosphate buffer, 10% v/v glycerol 8 Room temperature 2 mM 4-Vinylguaiacol Acetaldehyde , Vanillin Fe²⁺ Yes, "Vigorous shaking" Classical aqueous control (in %)
Activity - Classical Activity measured by HPLC (vanillin production measurement) in the presence of organic solvent Ethyl Acetate 10% 100.0 35.06 % 100 mM potassium phosphate buffer, 10% v/v glycerol 8 Room temperature 2 mM 4-Vinylguaiacol Acetaldehyde , Vanillin Fe²⁺ Yes, "Vigorous shaking" Classical aqueous control (in %)
Activity - Classical Activity measured by HPLC (vanillin production measurement) in the presence of organic solvent Toluene 10% 100.0 29.36 % 100 mM potassium phosphate buffer, 10% v/v glycerol 8 Room temperature 2 mM 4-Vinylguaiacol Acetaldehyde , Vanillin Fe²⁺ Yes, "Vigorous shaking" Classical aqueous control (in %)
Activity - Classical Activity measured by HPLC (vanillin production measurement) in the presence of organic solvent 1-Octanol 10% 100.0 144.66 % 100 mM potassium phosphate buffer, 10% v/v glycerol 8 Room temperature 2 mM 4-Vinylguaiacol Acetaldehyde , Vanillin Fe²⁺ Yes, "Vigorous shaking" Classical aqueous control (in %)
Activity - Classical Activity measured by HPLC (vanillin production measurement) in the presence of organic solvent Chloroform 10% 100.0 208.47 % 100 mM potassium phosphate buffer, 10% v/v glycerol 8 Room temperature 2 mM 4-Vinylguaiacol Acetaldehyde , Vanillin Fe²⁺ Yes, "Vigorous shaking" Classical aqueous control (in %)
Activity - Classical Activity measured by HPLC (vanillin production measurement) in the presence of organic solvent n-Octane 10% 100.0 55.34 % 100 mM potassium phosphate buffer, 10% v/v glycerol 8 Room temperature 2 mM Isoeugenol Acetaldehyde , Vanillin Fe²⁺ Yes, "Vigorous shaking" Classical aqueous control (in %)
Activity - Classical Activity measured by HPLC (vanillin production measurement) in the presence of organic solvent Dichloromethane 10% 100.0 121.97 % 100 mM potassium phosphate buffer, 10% v/v glycerol 8 Room temperature 2 mM 4-Vinylguaiacol Acetaldehyde , Vanillin Fe²⁺ Yes, "Vigorous shaking" Classical aqueous control (in %)
Activity - Classical Activity measured by HPLC (vanillin production measurement) in the presence of organic solvent 1-Octanol 10% 100.0 74.73 % 100 mM potassium phosphate buffer, 10% v/v glycerol 8 Room temperature 2 mM Isoeugenol Acetaldehyde , Vanillin Fe²⁺ Yes, "Vigorous shaking" Classical aqueous control (in %)
Activity - Classical Activity measured by HPLC (vanillin production measurement) in the presence of organic solvent Toluene 10% 100.0 39.56 % 100 mM potassium phosphate buffer, 10% v/v glycerol 8 Room temperature 2 mM Isoeugenol Acetaldehyde , Vanillin Fe²⁺ Yes, "Vigorous shaking" Classical aqueous control (in %)
Activity - Classical Activity measured by HPLC (vanillin production measurement) in the presence of organic solvent Ethyl Acetate 10% 100.0 19.35 % 100 mM potassium phosphate buffer, 10% v/v glycerol 8 Room temperature 2 mM Isoeugenol Acetaldehyde , Vanillin Fe²⁺ Yes, "Vigorous shaking" Classical aqueous control (in %)
Activity - Classical Activity measured by HPLC (vanillin production measurement) in the presence of organic solvent Cyclohexane 10% 100.0 91.01 % 100 mM potassium phosphate buffer, 10% v/v glycerol 8 Room temperature 2 mM Isoeugenol Acetaldehyde , Vanillin Fe²⁺ Yes, "Vigorous shaking" Classical aqueous control (in %)
Activity - Classical Activity measured by HPLC (vanillin production measurement) in the presence of organic solvent n-Hexane 10% 100.0 84.29 % 100 mM potassium phosphate buffer, 10% v/v glycerol 8 Room temperature 2 mM Isoeugenol Acetaldehyde , Vanillin Fe²⁺ Yes, "Vigorous shaking" Classical aqueous control (in %)
Activity - Classical Activity measured by HPLC (vanillin production measurement) in the presence of organic solvent Petroleum Ether 10% 100.0 46.05 % 100 mM potassium phosphate buffer, 10% v/v glycerol 8 Room temperature 2 mM Isoeugenol Acetaldehyde , Vanillin Fe²⁺ Yes, "Vigorous shaking" Classical aqueous control (in %)
Activity - Classical Activity measured by HPLC (vanillin production measurement) in the presence of organic solvent 1-Butanol 10% 100.0 38.19 % 100 mM potassium phosphate buffer, 10% v/v glycerol 8 Room temperature 2 mM Isoeugenol Acetaldehyde , Vanillin Fe²⁺ Yes, "Vigorous shaking" Classical aqueous control (in %)
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Visualization : Activity β€” Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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