9-cis-epoxycarotenoid dioxygenase (gene SeNCED) from Serratia sp. ATCC 39006

Enzyme Description

Species
Serratia sp. ATCC 39006 β†—
Extremophile
No
EC Number
1.13.11.51 β†—

Sequence

Length: 496 amino acids
MSLKFPETPEFTGLYKPSRVETQVFDLEIEGEVPPQIKGTFFQVSPDSYYPPMQGKDIFFNGDGLVSAFKFENGHVSLRRRYVQTDRLKAQWKERRSLNGIYRNIYTNDPLAAENNTTANTTVLFHAGVLLAMKEDALPYALNPNTLETLGVWDFNGQITSATFTAHPKIDPDNGDLLCFAYEAKGDGTSDIAYFEIDASGKLKKEIWFKGPYAAMIHDFAVTAHHVVFPLIPLTADVDRMKAGGQHFEWQPDLPQLFGVLPRDGSSEDVLWFHGPKDGFQGHTLNAYEEDGLLRVDMPVTNGNVFYFFPQADGSVPLPETLSSQLMRWTFNLNGPGESEAGQQTLQPQPLTSFPCEFPRCDERFTGKPYEHGFVLAFDPALPFDETLGERPFQFFNQLAHVNVRTGETETWYPGNAQCFQEPIFVPRSPDAPEGDGYVIALLNHLHGNDTELVVLDSLKMATGPVARIKVPFRLRMSLHGNWTPAEALKGTQSGR
Jiao Tang et al. (2018) β€” Expression and characterization of a 9-cis-epoxycarotenoid dioxygenase from Serratia sp. ATCC 39006 capable of biotransforming isoeugenol and 4-vinylguaiacol to vanillin
Biotechnology Reports  Β· doi:10.1016/j.btre.2018.e00253 β†—  Β· Activity - Classical Stability - Incubation
60 measurements ▼
Database ID
UniProt: A0A2I5TAF0 β†—
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (60 measurements)

60 measurements β€” page 3 of 3
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (2 hours at 20°C), measured by (HPLC vanillin production measurement) in aqueous phase Ethyl Acetate 50% 100.0 80.27 % Incubation: ?, Assay: 100 mM potassium phosphate buffer, 10% v/v glycerol Incubation: ?, Assay: 8 Incubation: 20°C, Assay: Room temperature 2 mM Isoeugenol Acetaldehyde , Vanillin Fe²⁺ Incubation: 200 rpm Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase, doubt about substrate nature, most proably isoeugenol but maybe 4-Vinylguaiacol
Stability - Incubation Activity after incubation (2 hours at 20°C), measured by (HPLC vanillin production measurement) in aqueous phase Dichloromethane 20% 100.0 85.88 % Incubation: ?, Assay: 100 mM potassium phosphate buffer, 10% v/v glycerol Incubation: ?, Assay: 8 Incubation: 20°C, Assay: Room temperature 2 mM Isoeugenol Acetaldehyde , Vanillin Fe²⁺ Incubation: 200 rpm Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase, doubt about substrate nature, most proably isoeugenol but maybe 4-Vinylguaiacol
Stability - Incubation Activity after incubation (2 hours at 20°C), measured by (HPLC vanillin production measurement) in aqueous phase Dichloromethane 50% 100.0 88.64 % Incubation: ?, Assay: 100 mM potassium phosphate buffer, 10% v/v glycerol Incubation: ?, Assay: 8 Incubation: 20°C, Assay: Room temperature 2 mM Isoeugenol Acetaldehyde , Vanillin Fe²⁺ Incubation: 200 rpm Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase, doubt about substrate nature, most proably isoeugenol but maybe 4-Vinylguaiacol
Stability - Incubation Activity after incubation (2 hours at 20°C), measured by (HPLC vanillin production measurement) in aqueous phase Chloroform 20% 100.0 88.74 % Incubation: ?, Assay: 100 mM potassium phosphate buffer, 10% v/v glycerol Incubation: ?, Assay: 8 Incubation: 20°C, Assay: Room temperature 2 mM Isoeugenol Acetaldehyde , Vanillin Fe²⁺ Incubation: 200 rpm Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase, doubt about substrate nature, most proably isoeugenol but maybe 4-Vinylguaiacol
Stability - Incubation Activity after incubation (2 hours at 20°C), measured by (HPLC vanillin production measurement) in aqueous phase Chloroform 50% 100.0 85.81 % Incubation: ?, Assay: 100 mM potassium phosphate buffer, 10% v/v glycerol Incubation: ?, Assay: 8 Incubation: 20°C, Assay: Room temperature 2 mM Isoeugenol Acetaldehyde , Vanillin Fe²⁺ Incubation: 200 rpm Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase, doubt about substrate nature, most proably isoeugenol but maybe 4-Vinylguaiacol
Stability - Incubation Activity after incubation (2 hours at 20°C), measured by (HPLC vanillin production measurement) in aqueous phase 1-Octanol 20% 100.0 87.14 % Incubation: ?, Assay: 100 mM potassium phosphate buffer, 10% v/v glycerol Incubation: ?, Assay: 8 Incubation: 20°C, Assay: Room temperature 2 mM Isoeugenol Acetaldehyde , Vanillin Fe²⁺ Incubation: 200 rpm Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase, doubt about substrate nature, most proably isoeugenol but maybe 4-Vinylguaiacol
Stability - Incubation Activity after incubation (2 hours at 20°C), measured by (HPLC vanillin production measurement) in aqueous phase 1-Octanol 50% 100.0 87.78 % Incubation: ?, Assay: 100 mM potassium phosphate buffer, 10% v/v glycerol Incubation: ?, Assay: 8 Incubation: 20°C, Assay: Room temperature 2 mM Isoeugenol Acetaldehyde , Vanillin Fe²⁺ Incubation: 200 rpm Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase, doubt about substrate nature, most proably isoeugenol but maybe 4-Vinylguaiacol
Stability - Incubation Activity after incubation (2 hours at 20°C), measured by (HPLC vanillin production measurement) in aqueous phase Toluene 20% 100.0 84.45 % Incubation: ?, Assay: 100 mM potassium phosphate buffer, 10% v/v glycerol Incubation: ?, Assay: 8 Incubation: 20°C, Assay: Room temperature 2 mM Isoeugenol Acetaldehyde , Vanillin Fe²⁺ Incubation: 200 rpm Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase, doubt about substrate nature, most proably isoeugenol but maybe 4-Vinylguaiacol
Stability - Incubation Activity after incubation (2 hours at 20°C), measured by (HPLC vanillin production measurement) in aqueous phase Toluene 50% 100.0 90.75 % Incubation: ?, Assay: 100 mM potassium phosphate buffer, 10% v/v glycerol Incubation: ?, Assay: 8 Incubation: 20°C, Assay: Room temperature 2 mM Isoeugenol Acetaldehyde , Vanillin Fe²⁺ Incubation: 200 rpm Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase, doubt about substrate nature, most proably isoeugenol but maybe 4-Vinylguaiacol
Stability - Incubation Activity after incubation (2 hours at 20°C), measured by (HPLC vanillin production measurement) in aqueous phase Ethyl Acetate 20% 100.0 85.54 % Incubation: ?, Assay: 100 mM potassium phosphate buffer, 10% v/v glycerol Incubation: ?, Assay: 8 Incubation: 20°C, Assay: Room temperature 2 mM Isoeugenol Acetaldehyde , Vanillin Fe²⁺ Incubation: 200 rpm Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase, doubt about substrate nature, most proably isoeugenol but maybe 4-Vinylguaiacol
Stability - Incubation Activity after incubation (2 hours at 20°C), measured by (HPLC vanillin production measurement) in aqueous phase n-Octane 50% 100.0 86.18 % Incubation: ?, Assay: 100 mM potassium phosphate buffer, 10% v/v glycerol Incubation: ?, Assay: 8 Incubation: 20°C, Assay: Room temperature 2 mM Isoeugenol Acetaldehyde , Vanillin Fe²⁺ Incubation: 200 rpm Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase, doubt about substrate nature, most proably isoeugenol but maybe 4-Vinylguaiacol
Stability - Incubation Activity after incubation (2 hours at 20°C), measured by (HPLC vanillin production measurement) in aqueous phase Cyclohexane 20% 100.0 86.2 % Incubation: ?, Assay: 100 mM potassium phosphate buffer, 10% v/v glycerol Incubation: ?, Assay: 8 Incubation: 20°C, Assay: Room temperature 2 mM Isoeugenol Acetaldehyde , Vanillin Fe²⁺ Incubation: 200 rpm Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase, doubt about substrate nature, most proably isoeugenol but maybe 4-Vinylguaiacol
Stability - Incubation Activity after incubation (2 hours at 20°C), measured by (HPLC vanillin production measurement) in aqueous phase Cyclohexane 50% 100.0 98.79 % Incubation: ?, Assay: 100 mM potassium phosphate buffer, 10% v/v glycerol Incubation: ?, Assay: 8 Incubation: 20°C, Assay: Room temperature 2 mM Isoeugenol Acetaldehyde , Vanillin Fe²⁺ Incubation: 200 rpm Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase, doubt about substrate nature, most proably isoeugenol but maybe 4-Vinylguaiacol
Stability - Incubation Activity after incubation (2 hours at 20°C), measured by (HPLC vanillin production measurement) in aqueous phase n-Hexane 20% 100.0 84.01 % Incubation: ?, Assay: 100 mM potassium phosphate buffer, 10% v/v glycerol Incubation: ?, Assay: 8 Incubation: 20°C, Assay: Room temperature 2 mM Isoeugenol Acetaldehyde , Vanillin Fe²⁺ Incubation: 200 rpm Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase, doubt about substrate nature, most proably isoeugenol but maybe 4-Vinylguaiacol
Stability - Incubation Activity after incubation (2 hours at 20°C), measured by (HPLC vanillin production measurement) in aqueous phase n-Hexane 50% 100.0 86.63 % Incubation: ?, Assay: 100 mM potassium phosphate buffer, 10% v/v glycerol Incubation: ?, Assay: 8 Incubation: 20°C, Assay: Room temperature 2 mM Isoeugenol Acetaldehyde , Vanillin Fe²⁺ Incubation: 200 rpm Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase, doubt about substrate nature, most proably isoeugenol but maybe 4-Vinylguaiacol
Stability - Incubation Activity after incubation (2 hours at 20°C), measured by (HPLC vanillin production measurement) in aqueous phase Petroleum Ether 20% 100.0 79.81 % Incubation: ?, Assay: 100 mM potassium phosphate buffer, 10% v/v glycerol Incubation: ?, Assay: 8 Incubation: 20°C, Assay: Room temperature 2 mM Isoeugenol Acetaldehyde , Vanillin Fe²⁺ Incubation: 200 rpm Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase, doubt about substrate nature, most proably isoeugenol but maybe 4-Vinylguaiacol
Stability - Incubation Activity after incubation (2 hours at 20°C), measured by (HPLC vanillin production measurement) in aqueous phase Petroleum Ether 50% 100.0 85.06 % Incubation: ?, Assay: 100 mM potassium phosphate buffer, 10% v/v glycerol Incubation: ?, Assay: 8 Incubation: 20°C, Assay: Room temperature 2 mM Isoeugenol Acetaldehyde , Vanillin Fe²⁺ Incubation: 200 rpm Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase, doubt about substrate nature, most proably isoeugenol but maybe 4-Vinylguaiacol
Stability - Incubation Activity after incubation (2 hours at 20°C), measured by (HPLC vanillin production measurement) in aqueous phase 1-Butanol 20% 100.0 46.41 % Incubation: ?, Assay: 100 mM potassium phosphate buffer, 10% v/v glycerol Incubation: ?, Assay: 8 Incubation: 20°C, Assay: Room temperature 2 mM Isoeugenol Acetaldehyde , Vanillin Fe²⁺ Incubation: 200 rpm Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase, doubt about substrate nature, most proably isoeugenol but maybe 4-Vinylguaiacol
Stability - Incubation Activity after incubation (2 hours at 20°C), measured by (HPLC vanillin production measurement) in aqueous phase 1-Butanol 50% 100.0 64.01 % Incubation: ?, Assay: 100 mM potassium phosphate buffer, 10% v/v glycerol Incubation: ?, Assay: 8 Incubation: 20°C, Assay: Room temperature 2 mM Isoeugenol Acetaldehyde , Vanillin Fe²⁺ Incubation: 200 rpm Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase, doubt about substrate nature, most proably isoeugenol but maybe 4-Vinylguaiacol
Stability - Incubation Activity after incubation (2 hours at 20°C), measured by (HPLC vanillin production measurement) in aqueous phase n-Octane 20% 100.0 80.78 % Incubation: ?, Assay: 100 mM potassium phosphate buffer, 10% v/v glycerol Incubation: ?, Assay: 8 Incubation: 20°C, Assay: Room temperature 2 mM Isoeugenol Acetaldehyde , Vanillin Fe²⁺ Incubation: 200 rpm Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase, doubt about substrate nature, most proably isoeugenol but maybe 4-Vinylguaiacol
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Visualization : Activity β€” Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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