Esterase (gene ThaEst2349) from Thalassospira sp. GB04J01

Enzyme Description

Species

Thalassospira sp. GB04J01 ↗

Extremophile

No but "psychrotolerant", "halo-tolerant" enzyme

EC Number

3.1.1.1 ↗

Sequence

Length: 343 amino acids

MSPLKTKVASAAIALTTALSANTALADPVLEPTTQKFINALSASGGPAIYTLTPAEARDVLSGAQSGEIAKPAVDITDTTFAVGPTGATKVRIIRPQGNTDRLPVIVYFHGAGWVMGDTGTHDRLVRELSVRANAALVFVDYERSPEARYPVAIEQDYAVTKYVAEHSEQLNVDPTRLAIAGDSVGGNMTAVVSLLAQERGGPDITAQVLFYPVTDADFDNGSYTEFANGPWLTKPAMDWFWNQYLPEGIDRTDPKITPIHATSEQLSGQAPALVITAENDVLRDEGEAYARKLSQAGVDVTVTRYNGTIHDFVMLNVLADTPAAKGAIAQAGQYLHTALHGK

Concetta De Santi et al. (2016) — Biochemical characterization and structural analysis of a new cold-active and salt-tolerant esterase from the marine bacterium Thalassospira sp.

Extremophiles · doi:10.1007/s00792-016-0824-z ↗ · Activity - Classical Stability - Incubation

38 measurements ▼

Database ID

UniProt: A0ACD6B8R4 ↗

Sequence Annotation

Explicit - Provided

Protein Source

Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (38 measurements)

38 measurements — page 1 of 2

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10%	100.0	104.0	%	100 mM Tris-HCl buffer, 3% acetonitrile	8	45°C	? mM p-Nitrophenol acetate	Acetic Acid , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Isopropanol	20%	100.0	3.0	%	100 mM Tris-HCl buffer, 3% acetonitrile	8	45°C	? mM p-Nitrophenol acetate	Acetic Acid , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Isopropanol	15%	100.0	4.0	%	100 mM Tris-HCl buffer, 3% acetonitrile	8	45°C	? mM p-Nitrophenol acetate	Acetic Acid , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Isopropanol	10%	100.0	5.0	%	100 mM Tris-HCl buffer, 3% acetonitrile	8	45°C	? mM p-Nitrophenol acetate	Acetic Acid , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Isopropanol	5%	100.0	14.0	%	100 mM Tris-HCl buffer, 3% acetonitrile	8	45°C	? mM p-Nitrophenol acetate	Acetic Acid , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	1-Butanol	20%	100.0	0.0	%	100 mM Tris-HCl buffer, 3% acetonitrile	8	45°C	? mM p-Nitrophenol acetate	Acetic Acid , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	1-Butanol	15%	100.0	0.0	%	100 mM Tris-HCl buffer, 3% acetonitrile	8	45°C	? mM p-Nitrophenol acetate	Acetic Acid , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	1-Butanol	10%	100.0	1.0	%	100 mM Tris-HCl buffer, 3% acetonitrile	8	45°C	? mM p-Nitrophenol acetate	Acetic Acid , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	1-Butanol	5%	100.0	6.0	%	100 mM Tris-HCl buffer, 3% acetonitrile	8	45°C	? mM p-Nitrophenol acetate	Acetic Acid , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	20%	100.0	2.0	%	100 mM Tris-HCl buffer, 3% acetonitrile	8	45°C	? mM p-Nitrophenol acetate	Acetic Acid , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	15%	100.0	3.0	%	100 mM Tris-HCl buffer, 3% acetonitrile	8	45°C	? mM p-Nitrophenol acetate	Acetic Acid , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	10%	100.0	7.0	%	100 mM Tris-HCl buffer, 3% acetonitrile	8	45°C	? mM p-Nitrophenol acetate	Acetic Acid , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	5%	100.0	21.0	%	100 mM Tris-HCl buffer, 3% acetonitrile	8	45°C	? mM p-Nitrophenol acetate	Acetic Acid , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Acetone	20%	100.0	2.0	%	100 mM Tris-HCl buffer, 3% acetonitrile	8	45°C	? mM p-Nitrophenol acetate	Acetic Acid , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Acetone	10%	100.0	18.0	%	100 mM Tris-HCl buffer, 3% acetonitrile	8	45°C	? mM p-Nitrophenol acetate	Acetic Acid , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	5%	100.0	106.0	%	100 mM Tris-HCl buffer, 3% acetonitrile	8	45°C	? mM p-Nitrophenol acetate	Acetic Acid , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	15%	100.0	82.0	%	100 mM Tris-HCl buffer, 3% acetonitrile	8	45°C	? mM p-Nitrophenol acetate	Acetic Acid , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	20%	100.0	78.0	%	100 mM Tris-HCl buffer, 3% acetonitrile	8	45°C	? mM p-Nitrophenol acetate	Acetic Acid , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Acetonitrile	5%	100.0	96.0	%	100 mM Tris-HCl buffer, 3% acetonitrile	8	45°C	? mM p-Nitrophenol acetate	Acetic Acid , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Acetonitrile	10%	100.0	33.0	%	100 mM Tris-HCl buffer, 3% acetonitrile	8	45°C	? mM p-Nitrophenol acetate	Acetic Acid , p-Nitrophenol	—	—	Classical aqueous control (in %)

Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10%	100.0	104.0	%	100 mM Tris-HCl buffer, 3% acetonitrile	8	45°C	? mM p-Nitrophenol acetate	Acetic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Isopropanol	20%	100.0	3.0	%	100 mM Tris-HCl buffer, 3% acetonitrile	8	45°C	? mM p-Nitrophenol acetate	Acetic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Isopropanol	15%	100.0	4.0	%	100 mM Tris-HCl buffer, 3% acetonitrile	8	45°C	? mM p-Nitrophenol acetate	Acetic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Isopropanol	10%	100.0	5.0	%	100 mM Tris-HCl buffer, 3% acetonitrile	8	45°C	? mM p-Nitrophenol acetate	Acetic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Isopropanol	5%	100.0	14.0	%	100 mM Tris-HCl buffer, 3% acetonitrile	8	45°C	? mM p-Nitrophenol acetate	Acetic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	1-Butanol	20%	100.0	0.0	%	100 mM Tris-HCl buffer, 3% acetonitrile	8	45°C	? mM p-Nitrophenol acetate	Acetic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	1-Butanol	15%	100.0	0.0	%	100 mM Tris-HCl buffer, 3% acetonitrile	8	45°C	? mM p-Nitrophenol acetate	Acetic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	1-Butanol	10%	100.0	1.0	%	100 mM Tris-HCl buffer, 3% acetonitrile	8	45°C	? mM p-Nitrophenol acetate	Acetic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	1-Butanol	5%	100.0	6.0	%	100 mM Tris-HCl buffer, 3% acetonitrile	8	45°C	? mM p-Nitrophenol acetate	Acetic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	20%	100.0	2.0	%	100 mM Tris-HCl buffer, 3% acetonitrile	8	45°C	? mM p-Nitrophenol acetate	Acetic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	15%	100.0	3.0	%	100 mM Tris-HCl buffer, 3% acetonitrile	8	45°C	? mM p-Nitrophenol acetate	Acetic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	10%	100.0	7.0	%	100 mM Tris-HCl buffer, 3% acetonitrile	8	45°C	? mM p-Nitrophenol acetate	Acetic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	5%	100.0	21.0	%	100 mM Tris-HCl buffer, 3% acetonitrile	8	45°C	? mM p-Nitrophenol acetate	Acetic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Acetone	20%	100.0	2.0	%	100 mM Tris-HCl buffer, 3% acetonitrile	8	45°C	? mM p-Nitrophenol acetate	Acetic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Acetone	10%	100.0	18.0	%	100 mM Tris-HCl buffer, 3% acetonitrile	8	45°C	? mM p-Nitrophenol acetate	Acetic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	5%	100.0	106.0	%	100 mM Tris-HCl buffer, 3% acetonitrile	8	45°C	? mM p-Nitrophenol acetate	Acetic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	15%	100.0	82.0	%	100 mM Tris-HCl buffer, 3% acetonitrile	8	45°C	? mM p-Nitrophenol acetate	Acetic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	20%	100.0	78.0	%	100 mM Tris-HCl buffer, 3% acetonitrile	8	45°C	? mM p-Nitrophenol acetate	Acetic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Acetonitrile	5%	100.0	96.0	%	100 mM Tris-HCl buffer, 3% acetonitrile	8	45°C	? mM p-Nitrophenol acetate	Acetic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Acetonitrile	10%	100.0	33.0	%	100 mM Tris-HCl buffer, 3% acetonitrile	8	45°C	? mM p-Nitrophenol acetate	Acetic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Acetonitrile	15%	100.0	13.0	%	100 mM Tris-HCl buffer, 3% acetonitrile	8	45°C	? mM p-Nitrophenol acetate	Acetic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Methanol	5%	100.0	26.0	%	100 mM Tris-HCl buffer, 3% acetonitrile	8	45°C	? mM p-Nitrophenol acetate	Acetic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Methanol	10%	100.0	9.0	%	100 mM Tris-HCl buffer, 3% acetonitrile	8	45°C	? mM p-Nitrophenol acetate	Acetic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Methanol	15%	100.0	5.0	%	100 mM Tris-HCl buffer, 3% acetonitrile	8	45°C	? mM p-Nitrophenol acetate	Acetic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Methanol	20%	100.0	5.0	%	100 mM Tris-HCl buffer, 3% acetonitrile	8	45°C	? mM p-Nitrophenol acetate	Acetic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Acetone	5%	100.0	60.0	%	100 mM Tris-HCl buffer, 3% acetonitrile	8	45°C	? mM p-Nitrophenol acetate	Acetic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Acetone	15%	100.0	9.0	%	100 mM Tris-HCl buffer, 3% acetonitrile	8	45°C	? mM p-Nitrophenol acetate	Acetic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Acetonitrile	20%	100.0	3.0	%	100 mM Tris-HCl buffer, 3% acetonitrile	8	45°C	? mM p-Nitrophenol acetate	Acetic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Diethyl Ether	10%	100.0	87.4	%	Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer, 3% acetonitrile	Incubation: 8, Assay: 8	Incubation: 4°C, Assay: 45°C	? mM p-Nitrophenol acetate	Acetic Acid, p-Nitrophenol	—	Incubation: 200 rpm	Non-incubated control (in %), assay measured in aqueous phase \| Uncertainty about control, uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Acetonitrile	10%	100.0	35.9	%	Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer, 3% acetonitrile	Incubation: 8, Assay: 8	Incubation: 4°C, Assay: 45°C	? mM p-Nitrophenol acetate	Acetic Acid, p-Nitrophenol	—	Incubation: 200 rpm	Non-incubated control (in %), assay measured in aqueous phase \| Uncertainty about control, uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Glycerol	10%	100.0	99.6	%	Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer, 3% acetonitrile	Incubation: 8, Assay: 8	Incubation: 4°C, Assay: 45°C	? mM p-Nitrophenol acetate	Acetic Acid, p-Nitrophenol	—	Incubation: 200 rpm	Non-incubated control (in %), assay measured in aqueous phase \| Uncertainty about control, uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Dimethylformamide (DMF)	10%	100.0	34.0	%	Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer, 3% acetonitrile	Incubation: 8, Assay: 8	Incubation: 4°C, Assay: 45°C	? mM p-Nitrophenol acetate	Acetic Acid, p-Nitrophenol	—	Incubation: 200 rpm	Non-incubated control (in %), assay measured in aqueous phase \| Uncertainty about control, uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	10%	100.0	108.7	%	Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer, 3% acetonitrile	Incubation: 8, Assay: 8	Incubation: 4°C, Assay: 45°C	? mM p-Nitrophenol acetate	Acetic Acid, p-Nitrophenol	—	Incubation: 200 rpm	Non-incubated control (in %), assay measured in aqueous phase \| Uncertainty about control, uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Ethanol	10%	100.0	10.1	%	Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer, 3% acetonitrile	Incubation: 8, Assay: 8	Incubation: 4°C, Assay: 45°C	? mM p-Nitrophenol acetate	Acetic Acid, p-Nitrophenol	—	Incubation: 200 rpm	Non-incubated control (in %), assay measured in aqueous phase \| Uncertainty about control, uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	1-Butanol	10%	100.0	41.9	%	Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer, 3% acetonitrile	Incubation: 8, Assay: 8	Incubation: 4°C, Assay: 45°C	? mM p-Nitrophenol acetate	Acetic Acid, p-Nitrophenol	—	Incubation: 200 rpm	Non-incubated control (in %), assay measured in aqueous phase \| Uncertainty about control, uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Isopropanol	10%	100.0	27.6	%	Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer, 3% acetonitrile	Incubation: 8, Assay: 8	Incubation: 4°C, Assay: 45°C	? mM p-Nitrophenol acetate	Acetic Acid, p-Nitrophenol	—	Incubation: 200 rpm	Non-incubated control (in %), assay measured in aqueous phase \| Uncertainty about control, uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Acetone	10%	100.0	42.9	%	Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer, 3% acetonitrile	Incubation: 8, Assay: 8	Incubation: 4°C, Assay: 45°C	? mM p-Nitrophenol acetate	Acetic Acid, p-Nitrophenol	—	Incubation: 200 rpm	Non-incubated control (in %), assay measured in aqueous phase \| Uncertainty about control, uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Methanol	10%	100.0	8.0	%	Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer, 3% acetonitrile	Incubation: 8, Assay: 8	Incubation: 4°C, Assay: 45°C	? mM p-Nitrophenol acetate	Acetic Acid, p-Nitrophenol	—	Incubation: 200 rpm	Non-incubated control (in %), assay measured in aqueous phase \| Uncertainty about control, uncertainty about whether assay was in the presence of organic solvent or in aqueous phase

‹ Prev

1 2

Next ›

Visualization : Activity — Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Visualization : Stability — Incubation