Esterase (gene ThaEst2349) from Thalassospira sp. GB04J01
Enzyme Description
Species
Extremophile
No
but "psychrotolerant", "halo-tolerant" enzyme
EC Number
Sequence
MSPLKTKVASAAIALTTALSANTALADPVLEPTTQKFINALSASGGPAIYTLTPAEARDVLSGAQSGEIAKPAVDITDTTFAVGPTGATKVRIIRPQGNTDRLPVIVYFHGAGWVMGDTGTHDRLVRELSVRANAALVFVDYERSPEARYPVAIEQDYAVTKYVAEHSEQLNVDPTRLAIAGDSVGGNMTAVVSLLAQERGGPDITAQVLFYPVTDADFDNGSYTEFANGPWLTKPAMDWFWNQYLPEGIDRTDPKITPIHATSEQLSGQAPALVITAENDVLRDEGEAYARKLSQAGVDVTVTRYNGTIHDFVMLNVLADTPAAKGAIAQAGQYLHTALHGK
Concetta De Santi et al. (2016)
β Biochemical characterization and structural analysis of a new cold-active and salt-tolerant esterase from the marine bacterium Thalassospira sp.
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Database ID
UniProt: A0ACD6B8R4 β
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)
Experimental Data (38 measurements)
38 measurements β page 2 of 2
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Acetonitrile | 15% | 100.0 | 13.0 | % | 100 mM Tris-HCl buffer, 3% acetonitrile | 8 | 45Β°C | ? mM p-Nitrophenol acetate | Acetic Acid , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Methanol | 5% | 100.0 | 26.0 | % | 100 mM Tris-HCl buffer, 3% acetonitrile | 8 | 45Β°C | ? mM p-Nitrophenol acetate | Acetic Acid , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Methanol | 10% | 100.0 | 9.0 | % | 100 mM Tris-HCl buffer, 3% acetonitrile | 8 | 45Β°C | ? mM p-Nitrophenol acetate | Acetic Acid , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Methanol | 15% | 100.0 | 5.0 | % | 100 mM Tris-HCl buffer, 3% acetonitrile | 8 | 45Β°C | ? mM p-Nitrophenol acetate | Acetic Acid , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Methanol | 20% | 100.0 | 5.0 | % | 100 mM Tris-HCl buffer, 3% acetonitrile | 8 | 45Β°C | ? mM p-Nitrophenol acetate | Acetic Acid , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Acetone | 5% | 100.0 | 60.0 | % | 100 mM Tris-HCl buffer, 3% acetonitrile | 8 | 45Β°C | ? mM p-Nitrophenol acetate | Acetic Acid , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Acetone | 15% | 100.0 | 9.0 | % | 100 mM Tris-HCl buffer, 3% acetonitrile | 8 | 45Β°C | ? mM p-Nitrophenol acetate | Acetic Acid , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Acetonitrile | 20% | 100.0 | 3.0 | % | 100 mM Tris-HCl buffer, 3% acetonitrile | 8 | 45Β°C | ? mM p-Nitrophenol acetate | Acetic Acid , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Stability - Incubation | Activity after incubation (24 hours at 4Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase | Diethyl Ether | 10% | 100.0 | 87.4 | % | Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer, 3% acetonitrile | Incubation: 8, Assay: 8 | Incubation: 4Β°C, Assay: 45Β°C | ? mM p-Nitrophenol acetate | Acetic Acid , p-Nitrophenol | β | Incubation: 200 rpm | Non-incubated control (in %), assay measured in aqueous phase | Uncertainty about control, uncertainty about whether assay was in the presence of organic solvent or in aqueous phase |
| Stability - Incubation | Activity after incubation (24 hours at 4Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase | Acetonitrile | 10% | 100.0 | 35.9 | % | Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer, 3% acetonitrile | Incubation: 8, Assay: 8 | Incubation: 4Β°C, Assay: 45Β°C | ? mM p-Nitrophenol acetate | Acetic Acid , p-Nitrophenol | β | Incubation: 200 rpm | Non-incubated control (in %), assay measured in aqueous phase | Uncertainty about control, uncertainty about whether assay was in the presence of organic solvent or in aqueous phase |
| Stability - Incubation | Activity after incubation (24 hours at 4Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase | Glycerol | 10% | 100.0 | 99.6 | % | Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer, 3% acetonitrile | Incubation: 8, Assay: 8 | Incubation: 4Β°C, Assay: 45Β°C | ? mM p-Nitrophenol acetate | Acetic Acid , p-Nitrophenol | β | Incubation: 200 rpm | Non-incubated control (in %), assay measured in aqueous phase | Uncertainty about control, uncertainty about whether assay was in the presence of organic solvent or in aqueous phase |
| Stability - Incubation | Activity after incubation (24 hours at 4Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase | Dimethylformamide (DMF) | 10% | 100.0 | 34.0 | % | Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer, 3% acetonitrile | Incubation: 8, Assay: 8 | Incubation: 4Β°C, Assay: 45Β°C | ? mM p-Nitrophenol acetate | Acetic Acid , p-Nitrophenol | β | Incubation: 200 rpm | Non-incubated control (in %), assay measured in aqueous phase | Uncertainty about control, uncertainty about whether assay was in the presence of organic solvent or in aqueous phase |
| Stability - Incubation | Activity after incubation (24 hours at 4Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 10% | 100.0 | 108.7 | % | Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer, 3% acetonitrile | Incubation: 8, Assay: 8 | Incubation: 4Β°C, Assay: 45Β°C | ? mM p-Nitrophenol acetate | Acetic Acid , p-Nitrophenol | β | Incubation: 200 rpm | Non-incubated control (in %), assay measured in aqueous phase | Uncertainty about control, uncertainty about whether assay was in the presence of organic solvent or in aqueous phase |
| Stability - Incubation | Activity after incubation (24 hours at 4Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase | Ethanol | 10% | 100.0 | 10.1 | % | Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer, 3% acetonitrile | Incubation: 8, Assay: 8 | Incubation: 4Β°C, Assay: 45Β°C | ? mM p-Nitrophenol acetate | Acetic Acid , p-Nitrophenol | β | Incubation: 200 rpm | Non-incubated control (in %), assay measured in aqueous phase | Uncertainty about control, uncertainty about whether assay was in the presence of organic solvent or in aqueous phase |
| Stability - Incubation | Activity after incubation (24 hours at 4Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase | 1-Butanol | 10% | 100.0 | 41.9 | % | Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer, 3% acetonitrile | Incubation: 8, Assay: 8 | Incubation: 4Β°C, Assay: 45Β°C | ? mM p-Nitrophenol acetate | Acetic Acid , p-Nitrophenol | β | Incubation: 200 rpm | Non-incubated control (in %), assay measured in aqueous phase | Uncertainty about control, uncertainty about whether assay was in the presence of organic solvent or in aqueous phase |
| Stability - Incubation | Activity after incubation (24 hours at 4Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase | Isopropanol | 10% | 100.0 | 27.6 | % | Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer, 3% acetonitrile | Incubation: 8, Assay: 8 | Incubation: 4Β°C, Assay: 45Β°C | ? mM p-Nitrophenol acetate | Acetic Acid , p-Nitrophenol | β | Incubation: 200 rpm | Non-incubated control (in %), assay measured in aqueous phase | Uncertainty about control, uncertainty about whether assay was in the presence of organic solvent or in aqueous phase |
| Stability - Incubation | Activity after incubation (24 hours at 4Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase | Acetone | 10% | 100.0 | 42.9 | % | Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer, 3% acetonitrile | Incubation: 8, Assay: 8 | Incubation: 4Β°C, Assay: 45Β°C | ? mM p-Nitrophenol acetate | Acetic Acid , p-Nitrophenol | β | Incubation: 200 rpm | Non-incubated control (in %), assay measured in aqueous phase | Uncertainty about control, uncertainty about whether assay was in the presence of organic solvent or in aqueous phase |
| Stability - Incubation | Activity after incubation (24 hours at 4Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase | Methanol | 10% | 100.0 | 8.0 | % | Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer, 3% acetonitrile | Incubation: 8, Assay: 8 | Incubation: 4Β°C, Assay: 45Β°C | ? mM p-Nitrophenol acetate | Acetic Acid , p-Nitrophenol | β | Incubation: 200 rpm | Non-incubated control (in %), assay measured in aqueous phase | Uncertainty about control, uncertainty about whether assay was in the presence of organic solvent or in aqueous phase |
Visualization : Activity β Classical
One bar per measurement. Colour = solvent, shade = solvent volume.
Visualization : Stability β Incubation
One bar per measurement. Colour = solvent, shade = solvent volume.