β-endoglucanase from Photobacterium panuliri LBS5T

Enzyme Description

Species

Photobacterium panuliri LBS5T ↗

Extremophile

No but "halostable" enzyme

EC Number

3.2.1.4 ↗

Sequence

Length: 442 amino acids

MACFYIFYIWSYLWLFICLQLFLVNTTTERLEMNNKIFLKDSEKTLTPVEKYGYLSINDDGLIYSESAKEVVQLKGLSFHGLGFGPEDPAVNPVGPKEFANRKAVINLAQMLKADIVRAPVYVDEWGGFFHTATGDDEKYSNQSVNRDNLYGRVAAIISGAVEAGLYVVIDWHILNVTIPDEAVGNSGAEAYHKTKYWQSKYSSYAPVDPDYPDIYEDTVHFFANMSRTYGDLPNVIFELANEPSKATLVESYEDWHNYVEPWAKEMIEVIRANDPDPDHPNLISVGTPTWSQYVGVAAESSIEDANVMYNAHIYAGSHLYPSGPNDKTLSEGERIRDEIIIARKAGKAVFISEWGSTTADGAATPDREQAMLWDEFFNHEGDANTPTMLLSSCYWNISSKDEASSIFNPDVVDPIGPWVSSNLSESGEIIRDILLNHTEED

Kamal Deep et al. (2015) — Cloning, Overexpression, and Characterization of Halostable, Solvent-Tolerant Novel β-Endoglucanase from a Marine Bacterium Photobacterium panuliri LBS5T (DSM 27646T)

Applied Biochemistry and Biotechnology · doi:10.1007/s12010-015-1903-9 ↗ · Stability - Incubation

65 measurements ▼

Database ID

UniProt: A0A0M4FQ56 ↗

Sequence Annotation

Explicit - Provided GenBank Accession Number

Protein Source

Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (65 measurements)

65 measurements — page 1 of 4

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Stability - Incubation	Activity after incubation (0 hour at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Acetone	50%	8.4	12.4	µM/min/mg	Incubation: ?, Assay: 40 mM phosphate buffer	Incubation: ?, Assay: 7	Incubation: Room temperature, Assay: 40°C	0.125% CMC (Carboxymethylcellulose sodium)	Reducing sugars	—	Incubation: 150 rpm	Water-incubated control (in microM/min/mg), assay measured in aqueous phase \| Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (18 hours at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	n-Hexane	50%	8.2	12.4	µM/min/mg	Incubation: ?, Assay: 40 mM phosphate buffer	Incubation: ?, Assay: 7	Incubation: Room temperature, Assay: 40°C	0.125% CMC (Carboxymethylcellulose sodium)	Reducing sugars	—	Incubation: 150 rpm	Water-incubated control (in microM/min/mg), assay measured in aqueous phase \| Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (12 hours at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	n-Hexane	50%	8.3	12.6	µM/min/mg	Incubation: ?, Assay: 40 mM phosphate buffer	Incubation: ?, Assay: 7	Incubation: Room temperature, Assay: 40°C	0.125% CMC (Carboxymethylcellulose sodium)	Reducing sugars	—	Incubation: 150 rpm	Water-incubated control (in microM/min/mg), assay measured in aqueous phase \| Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (6 hours at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	n-Hexane	50%	8.1	10.7	µM/min/mg	Incubation: ?, Assay: 40 mM phosphate buffer	Incubation: ?, Assay: 7	Incubation: Room temperature, Assay: 40°C	0.125% CMC (Carboxymethylcellulose sodium)	Reducing sugars	—	Incubation: 150 rpm	Water-incubated control (in microM/min/mg), assay measured in aqueous phase \| Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (0 hour at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	n-Hexane	50%	8.4	13.1	µM/min/mg	Incubation: ?, Assay: 40 mM phosphate buffer	Incubation: ?, Assay: 7	Incubation: Room temperature, Assay: 40°C	0.125% CMC (Carboxymethylcellulose sodium)	Reducing sugars	—	Incubation: 150 rpm	Water-incubated control (in microM/min/mg), assay measured in aqueous phase \| Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (24 hours at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Acetone	50%	8.0	11.8	µM/min/mg	Incubation: ?, Assay: 40 mM phosphate buffer	Incubation: ?, Assay: 7	Incubation: Room temperature, Assay: 40°C	0.125% CMC (Carboxymethylcellulose sodium)	Reducing sugars	—	Incubation: 150 rpm	Water-incubated control (in microM/min/mg), assay measured in aqueous phase \| Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (18 hours at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Acetone	50%	8.2	10.9	µM/min/mg	Incubation: ?, Assay: 40 mM phosphate buffer	Incubation: ?, Assay: 7	Incubation: Room temperature, Assay: 40°C	0.125% CMC (Carboxymethylcellulose sodium)	Reducing sugars	—	Incubation: 150 rpm	Water-incubated control (in microM/min/mg), assay measured in aqueous phase \| Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (12 hours at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Acetone	50%	8.3	12.1	µM/min/mg	Incubation: ?, Assay: 40 mM phosphate buffer	Incubation: ?, Assay: 7	Incubation: Room temperature, Assay: 40°C	0.125% CMC (Carboxymethylcellulose sodium)	Reducing sugars	—	Incubation: 150 rpm	Water-incubated control (in microM/min/mg), assay measured in aqueous phase \| Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (6 hours at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Acetone	50%	8.1	11.0	µM/min/mg	Incubation: ?, Assay: 40 mM phosphate buffer	Incubation: ?, Assay: 7	Incubation: Room temperature, Assay: 40°C	0.125% CMC (Carboxymethylcellulose sodium)	Reducing sugars	—	Incubation: 150 rpm	Water-incubated control (in microM/min/mg), assay measured in aqueous phase \| Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (24 hours at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	n-Hexane	50%	8.0	11.7	µM/min/mg	Incubation: ?, Assay: 40 mM phosphate buffer	Incubation: ?, Assay: 7	Incubation: Room temperature, Assay: 40°C	0.125% CMC (Carboxymethylcellulose sodium)	Reducing sugars	—	Incubation: 150 rpm	Water-incubated control (in microM/min/mg), assay measured in aqueous phase \| Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (24 hours at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Benzene	50%	8.0	11.6	µM/min/mg	Incubation: ?, Assay: 40 mM phosphate buffer	Incubation: ?, Assay: 7	Incubation: Room temperature, Assay: 40°C	0.125% CMC (Carboxymethylcellulose sodium)	Reducing sugars	—	Incubation: 150 rpm	Water-incubated control (in microM/min/mg), assay measured in aqueous phase \| Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (18 hours at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Benzene	50%	8.2	11.5	µM/min/mg	Incubation: ?, Assay: 40 mM phosphate buffer	Incubation: ?, Assay: 7	Incubation: Room temperature, Assay: 40°C	0.125% CMC (Carboxymethylcellulose sodium)	Reducing sugars	—	Incubation: 150 rpm	Water-incubated control (in microM/min/mg), assay measured in aqueous phase \| Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (12 hours at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Benzene	50%	8.3	11.5	µM/min/mg	Incubation: ?, Assay: 40 mM phosphate buffer	Incubation: ?, Assay: 7	Incubation: Room temperature, Assay: 40°C	0.125% CMC (Carboxymethylcellulose sodium)	Reducing sugars	—	Incubation: 150 rpm	Water-incubated control (in microM/min/mg), assay measured in aqueous phase \| Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (6 hours at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Benzene	50%	8.1	11.9	µM/min/mg	Incubation: ?, Assay: 40 mM phosphate buffer	Incubation: ?, Assay: 7	Incubation: Room temperature, Assay: 40°C	0.125% CMC (Carboxymethylcellulose sodium)	Reducing sugars	—	Incubation: 150 rpm	Water-incubated control (in microM/min/mg), assay measured in aqueous phase \| Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (0 hour at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Benzene	50%	8.4	12.9	µM/min/mg	Incubation: ?, Assay: 40 mM phosphate buffer	Incubation: ?, Assay: 7	Incubation: Room temperature, Assay: 40°C	0.125% CMC (Carboxymethylcellulose sodium)	Reducing sugars	—	Incubation: 150 rpm	Water-incubated control (in microM/min/mg), assay measured in aqueous phase \| Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (24 hours at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Petroleum Ether	50%	8.0	11.8	µM/min/mg	Incubation: ?, Assay: 40 mM phosphate buffer	Incubation: ?, Assay: 7	Incubation: Room temperature, Assay: 40°C	0.125% CMC (Carboxymethylcellulose sodium)	Reducing sugars	—	Incubation: 150 rpm	Water-incubated control (in microM/min/mg), assay measured in aqueous phase \| Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (18 hours at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Petroleum Ether	50%	8.2	12.1	µM/min/mg	Incubation: ?, Assay: 40 mM phosphate buffer	Incubation: ?, Assay: 7	Incubation: Room temperature, Assay: 40°C	0.125% CMC (Carboxymethylcellulose sodium)	Reducing sugars	—	Incubation: 150 rpm	Water-incubated control (in microM/min/mg), assay measured in aqueous phase \| Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (12 hours at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Petroleum Ether	50%	8.3	12.4	µM/min/mg	Incubation: ?, Assay: 40 mM phosphate buffer	Incubation: ?, Assay: 7	Incubation: Room temperature, Assay: 40°C	0.125% CMC (Carboxymethylcellulose sodium)	Reducing sugars	—	Incubation: 150 rpm	Water-incubated control (in microM/min/mg), assay measured in aqueous phase \| Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (0 hour at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Acetonitrile	50%	8.4	5.0	µM/min/mg	Incubation: ?, Assay: 40 mM phosphate buffer	Incubation: ?, Assay: 7	Incubation: Room temperature, Assay: 40°C	0.125% CMC (Carboxymethylcellulose sodium)	Reducing sugars	—	Incubation: 150 rpm	Water-incubated control (in microM/min/mg), assay measured in aqueous phase \| Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (6 hours at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Acetonitrile	50%	8.1	5.1	µM/min/mg	Incubation: ?, Assay: 40 mM phosphate buffer	Incubation: ?, Assay: 7	Incubation: Room temperature, Assay: 40°C	0.125% CMC (Carboxymethylcellulose sodium)	Reducing sugars	—	Incubation: 150 rpm	Water-incubated control (in microM/min/mg), assay measured in aqueous phase \| Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent

Stability - Incubation

Activity after incubation (0 hour at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase

Acetone

50%

8.4

12.4

µM/min/mg

Incubation: ?, Assay: 40 mM phosphate buffer

Incubation: ?, Assay: 7

Incubation: Room temperature, Assay: 40°C

0.125% CMC (Carboxymethylcellulose sodium)

Reducing sugars

—

Incubation: 150 rpm

Water-incubated control (in µM/min/mg), assay measured in aqueous phase | Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent

Stability - Incubation

Activity after incubation (18 hours at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase

n-Hexane

50%

8.2

12.4

µM/min/mg

Incubation: ?, Assay: 40 mM phosphate buffer

Incubation: ?, Assay: 7

Incubation: Room temperature, Assay: 40°C

0.125% CMC (Carboxymethylcellulose sodium)

Reducing sugars

—

Incubation: 150 rpm

Water-incubated control (in µM/min/mg), assay measured in aqueous phase | Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent

Stability - Incubation

Activity after incubation (12 hours at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase

n-Hexane

50%

8.3

12.6

µM/min/mg

Incubation: ?, Assay: 40 mM phosphate buffer

Incubation: ?, Assay: 7

Incubation: Room temperature, Assay: 40°C

0.125% CMC (Carboxymethylcellulose sodium)

Reducing sugars

—

Incubation: 150 rpm

Water-incubated control (in µM/min/mg), assay measured in aqueous phase | Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent

Stability - Incubation

Activity after incubation (6 hours at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase

n-Hexane

50%

8.1

10.7

µM/min/mg

Incubation: ?, Assay: 40 mM phosphate buffer

Incubation: ?, Assay: 7

Incubation: Room temperature, Assay: 40°C

0.125% CMC (Carboxymethylcellulose sodium)

Reducing sugars

—

Incubation: 150 rpm

Water-incubated control (in µM/min/mg), assay measured in aqueous phase | Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent

Stability - Incubation

Activity after incubation (0 hour at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase

n-Hexane

50%

8.4

13.1

µM/min/mg

Incubation: ?, Assay: 40 mM phosphate buffer

Incubation: ?, Assay: 7

Incubation: Room temperature, Assay: 40°C

0.125% CMC (Carboxymethylcellulose sodium)

Reducing sugars

—

Incubation: 150 rpm

Water-incubated control (in µM/min/mg), assay measured in aqueous phase | Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent

Stability - Incubation

Activity after incubation (24 hours at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase

Acetone

50%

8.0

11.8

µM/min/mg

Incubation: ?, Assay: 40 mM phosphate buffer

Incubation: ?, Assay: 7

Incubation: Room temperature, Assay: 40°C

0.125% CMC (Carboxymethylcellulose sodium)

Reducing sugars

—

Incubation: 150 rpm

Water-incubated control (in µM/min/mg), assay measured in aqueous phase | Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent

Stability - Incubation

Activity after incubation (18 hours at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase

Acetone

50%

8.2

10.9

µM/min/mg

Incubation: ?, Assay: 40 mM phosphate buffer

Incubation: ?, Assay: 7

Incubation: Room temperature, Assay: 40°C

0.125% CMC (Carboxymethylcellulose sodium)

Reducing sugars

—

Incubation: 150 rpm

Water-incubated control (in µM/min/mg), assay measured in aqueous phase | Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent

Stability - Incubation

Activity after incubation (12 hours at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase

Acetone

50%

8.3

12.1

µM/min/mg

Incubation: ?, Assay: 40 mM phosphate buffer

Incubation: ?, Assay: 7

Incubation: Room temperature, Assay: 40°C

0.125% CMC (Carboxymethylcellulose sodium)

Reducing sugars

—

Incubation: 150 rpm

Water-incubated control (in µM/min/mg), assay measured in aqueous phase | Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent

Stability - Incubation

Activity after incubation (6 hours at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase

Acetone

50%

8.1

11.0

µM/min/mg

Incubation: ?, Assay: 40 mM phosphate buffer

Incubation: ?, Assay: 7

Incubation: Room temperature, Assay: 40°C

0.125% CMC (Carboxymethylcellulose sodium)

Reducing sugars

—

Incubation: 150 rpm

Water-incubated control (in µM/min/mg), assay measured in aqueous phase | Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent

Stability - Incubation

Activity after incubation (24 hours at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase

n-Hexane

50%

8.0

11.7

µM/min/mg

Incubation: ?, Assay: 40 mM phosphate buffer

Incubation: ?, Assay: 7

Incubation: Room temperature, Assay: 40°C

0.125% CMC (Carboxymethylcellulose sodium)

Reducing sugars

—

Incubation: 150 rpm

Water-incubated control (in µM/min/mg), assay measured in aqueous phase | Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent

Stability - Incubation

Activity after incubation (24 hours at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase

Benzene

50%

8.0

11.6

µM/min/mg

Incubation: ?, Assay: 40 mM phosphate buffer

Incubation: ?, Assay: 7

Incubation: Room temperature, Assay: 40°C

0.125% CMC (Carboxymethylcellulose sodium)

Reducing sugars

—

Incubation: 150 rpm

Water-incubated control (in µM/min/mg), assay measured in aqueous phase | Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent

Stability - Incubation

Activity after incubation (18 hours at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase

Benzene

50%

8.2

11.5

µM/min/mg

Incubation: ?, Assay: 40 mM phosphate buffer

Incubation: ?, Assay: 7

Incubation: Room temperature, Assay: 40°C

0.125% CMC (Carboxymethylcellulose sodium)

Reducing sugars

—

Incubation: 150 rpm

Water-incubated control (in µM/min/mg), assay measured in aqueous phase | Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent

Stability - Incubation

Activity after incubation (12 hours at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase

Benzene

50%

8.3

11.5

µM/min/mg

Incubation: ?, Assay: 40 mM phosphate buffer

Incubation: ?, Assay: 7

Incubation: Room temperature, Assay: 40°C

0.125% CMC (Carboxymethylcellulose sodium)

Reducing sugars

—

Incubation: 150 rpm

Water-incubated control (in µM/min/mg), assay measured in aqueous phase | Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent

Stability - Incubation

Activity after incubation (6 hours at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase

Benzene

50%

8.1

11.9

µM/min/mg

Incubation: ?, Assay: 40 mM phosphate buffer

Incubation: ?, Assay: 7

Incubation: Room temperature, Assay: 40°C

0.125% CMC (Carboxymethylcellulose sodium)

Reducing sugars

—

Incubation: 150 rpm

Water-incubated control (in µM/min/mg), assay measured in aqueous phase | Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent

Stability - Incubation

Activity after incubation (0 hour at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase

Benzene

50%

8.4

12.9

µM/min/mg

Incubation: ?, Assay: 40 mM phosphate buffer

Incubation: ?, Assay: 7

Incubation: Room temperature, Assay: 40°C

0.125% CMC (Carboxymethylcellulose sodium)

Reducing sugars

—

Incubation: 150 rpm

Water-incubated control (in µM/min/mg), assay measured in aqueous phase | Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent

Stability - Incubation

Activity after incubation (24 hours at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase

Petroleum Ether

50%

8.0

11.8

µM/min/mg

Incubation: ?, Assay: 40 mM phosphate buffer

Incubation: ?, Assay: 7

Incubation: Room temperature, Assay: 40°C

0.125% CMC (Carboxymethylcellulose sodium)

Reducing sugars

—

Incubation: 150 rpm

Water-incubated control (in µM/min/mg), assay measured in aqueous phase | Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent

Stability - Incubation

Activity after incubation (18 hours at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase

Petroleum Ether

50%

8.2

12.1

µM/min/mg

Incubation: ?, Assay: 40 mM phosphate buffer

Incubation: ?, Assay: 7

Incubation: Room temperature, Assay: 40°C

0.125% CMC (Carboxymethylcellulose sodium)

Reducing sugars

—

Incubation: 150 rpm

Water-incubated control (in µM/min/mg), assay measured in aqueous phase | Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent

Stability - Incubation

Activity after incubation (12 hours at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase

Petroleum Ether

50%

8.3

12.4

µM/min/mg

Incubation: ?, Assay: 40 mM phosphate buffer

Incubation: ?, Assay: 7

Incubation: Room temperature, Assay: 40°C

0.125% CMC (Carboxymethylcellulose sodium)

Reducing sugars

—

Incubation: 150 rpm

Water-incubated control (in µM/min/mg), assay measured in aqueous phase | Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent

Stability - Incubation

Activity after incubation (0 hour at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase

Acetonitrile

50%

8.4

5.0

µM/min/mg

Incubation: ?, Assay: 40 mM phosphate buffer

Incubation: ?, Assay: 7

Incubation: Room temperature, Assay: 40°C

0.125% CMC (Carboxymethylcellulose sodium)

Reducing sugars

—

Incubation: 150 rpm

Water-incubated control (in µM/min/mg), assay measured in aqueous phase | Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent

Stability - Incubation

Activity after incubation (6 hours at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase

Acetonitrile

50%

8.1

5.1

µM/min/mg

Incubation: ?, Assay: 40 mM phosphate buffer

Incubation: ?, Assay: 7

Incubation: Room temperature, Assay: 40°C

0.125% CMC (Carboxymethylcellulose sodium)

Reducing sugars

—

Incubation: 150 rpm

Water-incubated control (in µM/min/mg), assay measured in aqueous phase | Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent

Stability - Incubation

Activity after incubation (12 hours at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase

Acetonitrile

50%

8.3

6.1

µM/min/mg

Incubation: ?, Assay: 40 mM phosphate buffer

Incubation: ?, Assay: 7

Incubation: Room temperature, Assay: 40°C

0.125% CMC (Carboxymethylcellulose sodium)

Reducing sugars

—

Incubation: 150 rpm

Water-incubated control (in µM/min/mg), assay measured in aqueous phase | Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent

Stability - Incubation

Activity after incubation (18 hours at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase

Acetonitrile

50%

8.2

3.1

µM/min/mg

Incubation: ?, Assay: 40 mM phosphate buffer

Incubation: ?, Assay: 7

Incubation: Room temperature, Assay: 40°C

0.125% CMC (Carboxymethylcellulose sodium)

Reducing sugars

—

Incubation: 150 rpm

Water-incubated control (in µM/min/mg), assay measured in aqueous phase | Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent

Stability - Incubation

Activity after incubation (24 hours at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase

Acetonitrile

50%

8.0

3.2

µM/min/mg

Incubation: ?, Assay: 40 mM phosphate buffer

Incubation: ?, Assay: 7

Incubation: Room temperature, Assay: 40°C

0.125% CMC (Carboxymethylcellulose sodium)

Reducing sugars

—

Incubation: 150 rpm

Water-incubated control (in µM/min/mg), assay measured in aqueous phase | Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent

Stability - Incubation

Activity after incubation (0 hour at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase

Formaldehyde

50%

8.4

0.0

µM/min/mg

Incubation: ?, Assay: 40 mM phosphate buffer

Incubation: ?, Assay: 7

Incubation: Room temperature, Assay: 40°C

0.125% CMC (Carboxymethylcellulose sodium)

Reducing sugars

—

Incubation: 150 rpm

Water-incubated control (in µM/min/mg), assay measured in aqueous phase | Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent

Stability - Incubation

Activity after incubation (6 hours at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase

Formaldehyde

50%

8.1

0.0

µM/min/mg

Incubation: ?, Assay: 40 mM phosphate buffer

Incubation: ?, Assay: 7

Incubation: Room temperature, Assay: 40°C

0.125% CMC (Carboxymethylcellulose sodium)

Reducing sugars

—

Incubation: 150 rpm

Water-incubated control (in µM/min/mg), assay measured in aqueous phase | Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent

Stability - Incubation

Activity after incubation (12 hours at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase

Formaldehyde

50%

8.3

0.0

µM/min/mg

Incubation: ?, Assay: 40 mM phosphate buffer

Incubation: ?, Assay: 7

Incubation: Room temperature, Assay: 40°C

0.125% CMC (Carboxymethylcellulose sodium)

Reducing sugars

—

Incubation: 150 rpm

Water-incubated control (in µM/min/mg), assay measured in aqueous phase | Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent

Stability - Incubation

Activity after incubation (18 hours at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase

Formaldehyde

50%

8.2

0.0

µM/min/mg

Incubation: ?, Assay: 40 mM phosphate buffer

Incubation: ?, Assay: 7

Incubation: Room temperature, Assay: 40°C

0.125% CMC (Carboxymethylcellulose sodium)

Reducing sugars

—

Incubation: 150 rpm

Water-incubated control (in µM/min/mg), assay measured in aqueous phase | Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent

Stability - Incubation

Activity after incubation (24 hours at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase

Formaldehyde

50%

8.0

0.0

µM/min/mg

Incubation: ?, Assay: 40 mM phosphate buffer

Incubation: ?, Assay: 7

Incubation: Room temperature, Assay: 40°C

0.125% CMC (Carboxymethylcellulose sodium)

Reducing sugars

—

Incubation: 150 rpm

Water-incubated control (in µM/min/mg), assay measured in aqueous phase | Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent

Stability - Incubation

Activity after incubation (0 hour at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase

Chloroform

50%

8.4

0.0

µM/min/mg

Incubation: ?, Assay: 40 mM phosphate buffer

Incubation: ?, Assay: 7

Incubation: Room temperature, Assay: 40°C

0.125% CMC (Carboxymethylcellulose sodium)

Reducing sugars

—

Incubation: 150 rpm

Water-incubated control (in µM/min/mg), assay measured in aqueous phase | Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent

Stability - Incubation

Activity after incubation (6 hours at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase

Chloroform

50%

8.1

0.0

µM/min/mg

Incubation: ?, Assay: 40 mM phosphate buffer

Incubation: ?, Assay: 7

Incubation: Room temperature, Assay: 40°C

0.125% CMC (Carboxymethylcellulose sodium)

Reducing sugars

—

Incubation: 150 rpm

Water-incubated control (in µM/min/mg), assay measured in aqueous phase | Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent

Stability - Incubation

Activity after incubation (12 hours at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase

Chloroform

50%

8.3

0.0

µM/min/mg

Incubation: ?, Assay: 40 mM phosphate buffer

Incubation: ?, Assay: 7

Incubation: Room temperature, Assay: 40°C

0.125% CMC (Carboxymethylcellulose sodium)

Reducing sugars

—

Incubation: 150 rpm

Water-incubated control (in µM/min/mg), assay measured in aqueous phase | Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent

Stability - Incubation

Activity after incubation (18 hours at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase

Chloroform

50%

8.2

0.0

µM/min/mg

Incubation: ?, Assay: 40 mM phosphate buffer

Incubation: ?, Assay: 7

Incubation: Room temperature, Assay: 40°C

0.125% CMC (Carboxymethylcellulose sodium)

Reducing sugars

—

Incubation: 150 rpm

Water-incubated control (in µM/min/mg), assay measured in aqueous phase | Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent

Stability - Incubation

Activity after incubation (24 hours at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase

Chloroform

50%

8.0

0.0

µM/min/mg

Incubation: ?, Assay: 40 mM phosphate buffer

Incubation: ?, Assay: 7

Incubation: Room temperature, Assay: 40°C

0.125% CMC (Carboxymethylcellulose sodium)

Reducing sugars

—

Incubation: 150 rpm

Water-incubated control (in µM/min/mg), assay measured in aqueous phase | Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent

Stability - Incubation

Activity after incubation (6 hours at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase

Toluene

50%

8.1

10.6

µM/min/mg

Incubation: ?, Assay: 40 mM phosphate buffer

Incubation: ?, Assay: 7

Incubation: Room temperature, Assay: 40°C

0.125% CMC (Carboxymethylcellulose sodium)

Reducing sugars

—

Incubation: 150 rpm

Water-incubated control (in µM/min/mg), assay measured in aqueous phase | Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent

Stability - Incubation

Activity after incubation (6 hours at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase

Dimethyl Sulfoxide (DMSO)

50%

8.1

6.8

µM/min/mg

Incubation: ?, Assay: 40 mM phosphate buffer

Incubation: ?, Assay: 7

Incubation: Room temperature, Assay: 40°C

0.125% CMC (Carboxymethylcellulose sodium)

Reducing sugars

—

Incubation: 150 rpm

Water-incubated control (in µM/min/mg), assay measured in aqueous phase | Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent

Stability - Incubation

Activity after incubation (12 hours at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase

Dimethyl Sulfoxide (DMSO)

50%

8.3

7.3

µM/min/mg

Incubation: ?, Assay: 40 mM phosphate buffer

Incubation: ?, Assay: 7

Incubation: Room temperature, Assay: 40°C

0.125% CMC (Carboxymethylcellulose sodium)

Reducing sugars

—

Incubation: 150 rpm

Water-incubated control (in µM/min/mg), assay measured in aqueous phase | Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent

Stability - Incubation

Activity after incubation (18 hours at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase

Dimethyl Sulfoxide (DMSO)

50%

8.2

7.2

µM/min/mg

Incubation: ?, Assay: 40 mM phosphate buffer

Incubation: ?, Assay: 7

Incubation: Room temperature, Assay: 40°C

0.125% CMC (Carboxymethylcellulose sodium)

Reducing sugars

—

Incubation: 150 rpm

Water-incubated control (in µM/min/mg), assay measured in aqueous phase | Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent

Stability - Incubation

Activity after incubation (24 hours at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase

Dimethyl Sulfoxide (DMSO)

50%

8.0

7.5

µM/min/mg

Incubation: ?, Assay: 40 mM phosphate buffer

Incubation: ?, Assay: 7

Incubation: Room temperature, Assay: 40°C

0.125% CMC (Carboxymethylcellulose sodium)

Reducing sugars

—

Incubation: 150 rpm

Water-incubated control (in µM/min/mg), assay measured in aqueous phase | Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent

Stability - Incubation

Activity after incubation (0 hour at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase

1-Butanol

50%

8.4

13.5

µM/min/mg

Incubation: ?, Assay: 40 mM phosphate buffer

Incubation: ?, Assay: 7

Incubation: Room temperature, Assay: 40°C

0.125% CMC (Carboxymethylcellulose sodium)

Reducing sugars

—

Incubation: 150 rpm

Water-incubated control (in µM/min/mg), assay measured in aqueous phase | Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent

Stability - Incubation

Activity after incubation (6 hours at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase

1-Butanol

50%

8.1

13.7

µM/min/mg

Incubation: ?, Assay: 40 mM phosphate buffer

Incubation: ?, Assay: 7

Incubation: Room temperature, Assay: 40°C

0.125% CMC (Carboxymethylcellulose sodium)

Reducing sugars

—

Incubation: 150 rpm

Water-incubated control (in µM/min/mg), assay measured in aqueous phase | Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent

Stability - Incubation

Activity after incubation (12 hours at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase

1-Butanol

50%

8.3

12.3

µM/min/mg

Incubation: ?, Assay: 40 mM phosphate buffer

Incubation: ?, Assay: 7

Incubation: Room temperature, Assay: 40°C

0.125% CMC (Carboxymethylcellulose sodium)

Reducing sugars

—

Incubation: 150 rpm

Water-incubated control (in µM/min/mg), assay measured in aqueous phase | Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent

Stability - Incubation

Activity after incubation (18 hours at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase

1-Butanol

50%

8.2

13.0

µM/min/mg

Incubation: ?, Assay: 40 mM phosphate buffer

Incubation: ?, Assay: 7

Incubation: Room temperature, Assay: 40°C

0.125% CMC (Carboxymethylcellulose sodium)

Reducing sugars

—

Incubation: 150 rpm

Water-incubated control (in µM/min/mg), assay measured in aqueous phase | Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent

Stability - Incubation

Activity after incubation (24 hours at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase

1-Butanol

50%

8.0

12.0

µM/min/mg

Incubation: ?, Assay: 40 mM phosphate buffer

Incubation: ?, Assay: 7

Incubation: Room temperature, Assay: 40°C

0.125% CMC (Carboxymethylcellulose sodium)

Reducing sugars

—

Incubation: 150 rpm

Water-incubated control (in µM/min/mg), assay measured in aqueous phase | Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent

Stability - Incubation

Activity after incubation (0 hour at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase

Isopropanol

50%

8.4

13.4

µM/min/mg

Incubation: ?, Assay: 40 mM phosphate buffer

Incubation: ?, Assay: 7

Incubation: Room temperature, Assay: 40°C

0.125% CMC (Carboxymethylcellulose sodium)

Reducing sugars

—

Incubation: 150 rpm

Water-incubated control (in µM/min/mg), assay measured in aqueous phase | Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent

Stability - Incubation

Activity after incubation (6 hours at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase

Isopropanol

50%

8.1

11.0

µM/min/mg

Incubation: ?, Assay: 40 mM phosphate buffer

Incubation: ?, Assay: 7

Incubation: Room temperature, Assay: 40°C

0.125% CMC (Carboxymethylcellulose sodium)

Reducing sugars

—

Incubation: 150 rpm

Water-incubated control (in µM/min/mg), assay measured in aqueous phase | Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent

Stability - Incubation

Activity after incubation (12 hours at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase

Isopropanol

50%

8.3

12.0

µM/min/mg

Incubation: ?, Assay: 40 mM phosphate buffer

Incubation: ?, Assay: 7

Incubation: Room temperature, Assay: 40°C

0.125% CMC (Carboxymethylcellulose sodium)

Reducing sugars

—

Incubation: 150 rpm

Water-incubated control (in µM/min/mg), assay measured in aqueous phase | Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent

Stability - Incubation

Activity after incubation (18 hours at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase

Isopropanol

50%

8.2

8.9

µM/min/mg

Incubation: ?, Assay: 40 mM phosphate buffer

Incubation: ?, Assay: 7

Incubation: Room temperature, Assay: 40°C

0.125% CMC (Carboxymethylcellulose sodium)

Reducing sugars

—

Incubation: 150 rpm

Water-incubated control (in µM/min/mg), assay measured in aqueous phase | Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent

Stability - Incubation

Activity after incubation (24 hours at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase

Isopropanol

50%

8.0

12.1

µM/min/mg

Incubation: ?, Assay: 40 mM phosphate buffer

Incubation: ?, Assay: 7

Incubation: Room temperature, Assay: 40°C

0.125% CMC (Carboxymethylcellulose sodium)

Reducing sugars

—

Incubation: 150 rpm

Water-incubated control (in µM/min/mg), assay measured in aqueous phase | Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent

Stability - Incubation

Activity after incubation (0 hour at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase

Toluene

50%

8.4

8.9

µM/min/mg

Incubation: ?, Assay: 40 mM phosphate buffer

Incubation: ?, Assay: 7

Incubation: Room temperature, Assay: 40°C

0.125% CMC (Carboxymethylcellulose sodium)

Reducing sugars

—

Incubation: 150 rpm

Water-incubated control (in µM/min/mg), assay measured in aqueous phase | Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent

Stability - Incubation

Activity after incubation (0 hour at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase

Dimethyl Sulfoxide (DMSO)

50%

8.4

7.6

µM/min/mg

Incubation: ?, Assay: 40 mM phosphate buffer

Incubation: ?, Assay: 7

Incubation: Room temperature, Assay: 40°C

0.125% CMC (Carboxymethylcellulose sodium)

Reducing sugars

—

Incubation: 150 rpm

Water-incubated control (in µM/min/mg), assay measured in aqueous phase | Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent

Stability - Incubation

Activity after incubation (12 hours at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase

Toluene

50%

8.3

8.2

µM/min/mg

Incubation: ?, Assay: 40 mM phosphate buffer

Incubation: ?, Assay: 7

Incubation: Room temperature, Assay: 40°C

0.125% CMC (Carboxymethylcellulose sodium)

Reducing sugars

—

Incubation: 150 rpm

Water-incubated control (in µM/min/mg), assay measured in aqueous phase | Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent

Stability - Incubation

Activity after incubation (18 hours at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase

Toluene

50%

8.2

8.6

µM/min/mg

Incubation: ?, Assay: 40 mM phosphate buffer

Incubation: ?, Assay: 7

Incubation: Room temperature, Assay: 40°C

0.125% CMC (Carboxymethylcellulose sodium)

Reducing sugars

—

Incubation: 150 rpm

Water-incubated control (in µM/min/mg), assay measured in aqueous phase | Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent

Stability - Incubation

Activity after incubation (24 hours at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase

Toluene

50%

8.0

9.6

µM/min/mg

Incubation: ?, Assay: 40 mM phosphate buffer

Incubation: ?, Assay: 7

Incubation: Room temperature, Assay: 40°C

0.125% CMC (Carboxymethylcellulose sodium)

Reducing sugars

—

Incubation: 150 rpm

Water-incubated control (in µM/min/mg), assay measured in aqueous phase | Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent

Stability - Incubation

Activity after incubation (0 hour at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase

Methanol

50%

8.4

10.5

µM/min/mg

Incubation: ?, Assay: 40 mM phosphate buffer

Incubation: ?, Assay: 7

Incubation: Room temperature, Assay: 40°C

0.125% CMC (Carboxymethylcellulose sodium)

Reducing sugars

—

Incubation: 150 rpm

Water-incubated control (in µM/min/mg), assay measured in aqueous phase | Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent

Stability - Incubation

Activity after incubation (6 hours at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase

Methanol

50%

8.1

7.8

µM/min/mg

Incubation: ?, Assay: 40 mM phosphate buffer

Incubation: ?, Assay: 7

Incubation: Room temperature, Assay: 40°C

0.125% CMC (Carboxymethylcellulose sodium)

Reducing sugars

—

Incubation: 150 rpm

Water-incubated control (in µM/min/mg), assay measured in aqueous phase | Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent

Stability - Incubation

Activity after incubation (12 hours at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase

Methanol

50%

8.3

7.7

µM/min/mg

Incubation: ?, Assay: 40 mM phosphate buffer

Incubation: ?, Assay: 7

Incubation: Room temperature, Assay: 40°C

0.125% CMC (Carboxymethylcellulose sodium)

Reducing sugars

—

Incubation: 150 rpm

Water-incubated control (in µM/min/mg), assay measured in aqueous phase | Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent

Stability - Incubation

Activity after incubation (18 hours at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase

Methanol

50%

8.2

7.8

µM/min/mg

Incubation: ?, Assay: 40 mM phosphate buffer

Incubation: ?, Assay: 7

Incubation: Room temperature, Assay: 40°C

0.125% CMC (Carboxymethylcellulose sodium)

Reducing sugars

—

Incubation: 150 rpm

Water-incubated control (in µM/min/mg), assay measured in aqueous phase | Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent

Stability - Incubation

Activity after incubation (24 hours at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase

Methanol

50%

8.0

8.2

µM/min/mg

Incubation: ?, Assay: 40 mM phosphate buffer

Incubation: ?, Assay: 7

Incubation: Room temperature, Assay: 40°C

0.125% CMC (Carboxymethylcellulose sodium)

Reducing sugars

—

Incubation: 150 rpm

Water-incubated control (in µM/min/mg), assay measured in aqueous phase | Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent

Stability - Incubation

Activity after incubation (0 hour at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase

Ethanol

50%

8.4

11.2

µM/min/mg

Incubation: ?, Assay: 40 mM phosphate buffer

Incubation: ?, Assay: 7

Incubation: Room temperature, Assay: 40°C

0.125% CMC (Carboxymethylcellulose sodium)

Reducing sugars

—

Incubation: 150 rpm

Water-incubated control (in µM/min/mg), assay measured in aqueous phase | Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent

Stability - Incubation

Activity after incubation (6 hours at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase

Ethanol

50%

8.1

7.8

µM/min/mg

Incubation: ?, Assay: 40 mM phosphate buffer

Incubation: ?, Assay: 7

Incubation: Room temperature, Assay: 40°C

0.125% CMC (Carboxymethylcellulose sodium)

Reducing sugars

—

Incubation: 150 rpm

Water-incubated control (in µM/min/mg), assay measured in aqueous phase | Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent

Stability - Incubation

Activity after incubation (12 hours at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase

Ethanol

50%

8.3

8.1

µM/min/mg

Incubation: ?, Assay: 40 mM phosphate buffer

Incubation: ?, Assay: 7

Incubation: Room temperature, Assay: 40°C

0.125% CMC (Carboxymethylcellulose sodium)

Reducing sugars

—

Incubation: 150 rpm

Water-incubated control (in µM/min/mg), assay measured in aqueous phase | Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent

Stability - Incubation

Activity after incubation (18 hours at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase

Ethanol

50%

8.2

7.3

µM/min/mg

Incubation: ?, Assay: 40 mM phosphate buffer

Incubation: ?, Assay: 7

Incubation: Room temperature, Assay: 40°C

0.125% CMC (Carboxymethylcellulose sodium)

Reducing sugars

—

Incubation: 150 rpm

Water-incubated control (in µM/min/mg), assay measured in aqueous phase | Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent

Stability - Incubation

Activity after incubation (24 hours at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase

Ethanol

50%

8.0

6.9

µM/min/mg

Incubation: ?, Assay: 40 mM phosphate buffer

Incubation: ?, Assay: 7

Incubation: Room temperature, Assay: 40°C

0.125% CMC (Carboxymethylcellulose sodium)

Reducing sugars

—

Incubation: 150 rpm

Water-incubated control (in µM/min/mg), assay measured in aqueous phase | Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent

Stability - Incubation

Activity after incubation (0 hour at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase

Petroleum Ether

50%

8.4

12.2

µM/min/mg

Incubation: ?, Assay: 40 mM phosphate buffer

Incubation: ?, Assay: 7

Incubation: Room temperature, Assay: 40°C

0.125% CMC (Carboxymethylcellulose sodium)

Reducing sugars

—

Incubation: 150 rpm

Water-incubated control (in µM/min/mg), assay measured in aqueous phase | Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent

Stability - Incubation

Activity after incubation (6 hours at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase

Petroleum Ether

50%

8.1

10.1

µM/min/mg

Incubation: ?, Assay: 40 mM phosphate buffer

Incubation: ?, Assay: 7

Incubation: Room temperature, Assay: 40°C

0.125% CMC (Carboxymethylcellulose sodium)

Reducing sugars

—

Incubation: 150 rpm

Water-incubated control (in µM/min/mg), assay measured in aqueous phase | Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent

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Visualization : Stability — Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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β-endoglucanase from Photobacterium panuliri LBS5T

Enzyme Description

Sequence

Experimental Data (65 measurements)

Visualization : Stability — Incubation

Structure

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