Ξ²-endoglucanase from Photobacterium panuliri LBS5T

Enzyme Description

Species
Photobacterium panuliri LBS5T β†—
Extremophile
No but "halostable" enzyme
EC Number
3.2.1.4 β†—

Sequence

Length: 442 amino acids
MACFYIFYIWSYLWLFICLQLFLVNTTTERLEMNNKIFLKDSEKTLTPVEKYGYLSINDDGLIYSESAKEVVQLKGLSFHGLGFGPEDPAVNPVGPKEFANRKAVINLAQMLKADIVRAPVYVDEWGGFFHTATGDDEKYSNQSVNRDNLYGRVAAIISGAVEAGLYVVIDWHILNVTIPDEAVGNSGAEAYHKTKYWQSKYSSYAPVDPDYPDIYEDTVHFFANMSRTYGDLPNVIFELANEPSKATLVESYEDWHNYVEPWAKEMIEVIRANDPDPDHPNLISVGTPTWSQYVGVAAESSIEDANVMYNAHIYAGSHLYPSGPNDKTLSEGERIRDEIIIARKAGKAVFISEWGSTTADGAATPDREQAMLWDEFFNHEGDANTPTMLLSSCYWNISSKDEASSIFNPDVVDPIGPWVSSNLSESGEIIRDILLNHTEED
Kamal Deep et al. (2015) β€” Cloning, Overexpression, and Characterization of Halostable, Solvent-Tolerant Novel Ξ²-Endoglucanase from a Marine Bacterium Photobacterium panuliri LBS5T (DSM 27646T)
Applied Biochemistry and Biotechnology  Β· doi:10.1007/s12010-015-1903-9 β†—  Β· Stability - Incubation
65 measurements ▼
Database ID
UniProt: A0A0M4FQ56 β†—
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (65 measurements)

65 measurements β€” page 2 of 4
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (12 hours at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Acetonitrile 50% 8.3 6.1 Β΅M/min/mg Incubation: ?, Assay: 40 mM phosphate buffer Incubation: ?, Assay: 7 Incubation: Room temperature, Assay: 40Β°C 0.125% CMC (Carboxymethylcellulose sodium) Reducing sugars β€” Incubation: 150 rpm Water-incubated control (in microM/min/mg), assay measured in aqueous phase | Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation Activity after incubation (18 hours at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Acetonitrile 50% 8.2 3.1 Β΅M/min/mg Incubation: ?, Assay: 40 mM phosphate buffer Incubation: ?, Assay: 7 Incubation: Room temperature, Assay: 40Β°C 0.125% CMC (Carboxymethylcellulose sodium) Reducing sugars β€” Incubation: 150 rpm Water-incubated control (in microM/min/mg), assay measured in aqueous phase | Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation Activity after incubation (24 hours at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Acetonitrile 50% 8.0 3.2 Β΅M/min/mg Incubation: ?, Assay: 40 mM phosphate buffer Incubation: ?, Assay: 7 Incubation: Room temperature, Assay: 40Β°C 0.125% CMC (Carboxymethylcellulose sodium) Reducing sugars β€” Incubation: 150 rpm Water-incubated control (in microM/min/mg), assay measured in aqueous phase | Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation Activity after incubation (0 hour at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Formaldehyde 50% 8.4 0.0 Β΅M/min/mg Incubation: ?, Assay: 40 mM phosphate buffer Incubation: ?, Assay: 7 Incubation: Room temperature, Assay: 40Β°C 0.125% CMC (Carboxymethylcellulose sodium) Reducing sugars β€” Incubation: 150 rpm Water-incubated control (in microM/min/mg), assay measured in aqueous phase | Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation Activity after incubation (6 hours at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Formaldehyde 50% 8.1 0.0 Β΅M/min/mg Incubation: ?, Assay: 40 mM phosphate buffer Incubation: ?, Assay: 7 Incubation: Room temperature, Assay: 40Β°C 0.125% CMC (Carboxymethylcellulose sodium) Reducing sugars β€” Incubation: 150 rpm Water-incubated control (in microM/min/mg), assay measured in aqueous phase | Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation Activity after incubation (12 hours at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Formaldehyde 50% 8.3 0.0 Β΅M/min/mg Incubation: ?, Assay: 40 mM phosphate buffer Incubation: ?, Assay: 7 Incubation: Room temperature, Assay: 40Β°C 0.125% CMC (Carboxymethylcellulose sodium) Reducing sugars β€” Incubation: 150 rpm Water-incubated control (in microM/min/mg), assay measured in aqueous phase | Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation Activity after incubation (18 hours at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Formaldehyde 50% 8.2 0.0 Β΅M/min/mg Incubation: ?, Assay: 40 mM phosphate buffer Incubation: ?, Assay: 7 Incubation: Room temperature, Assay: 40Β°C 0.125% CMC (Carboxymethylcellulose sodium) Reducing sugars β€” Incubation: 150 rpm Water-incubated control (in microM/min/mg), assay measured in aqueous phase | Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation Activity after incubation (24 hours at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Formaldehyde 50% 8.0 0.0 Β΅M/min/mg Incubation: ?, Assay: 40 mM phosphate buffer Incubation: ?, Assay: 7 Incubation: Room temperature, Assay: 40Β°C 0.125% CMC (Carboxymethylcellulose sodium) Reducing sugars β€” Incubation: 150 rpm Water-incubated control (in microM/min/mg), assay measured in aqueous phase | Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation Activity after incubation (0 hour at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Chloroform 50% 8.4 0.0 Β΅M/min/mg Incubation: ?, Assay: 40 mM phosphate buffer Incubation: ?, Assay: 7 Incubation: Room temperature, Assay: 40Β°C 0.125% CMC (Carboxymethylcellulose sodium) Reducing sugars β€” Incubation: 150 rpm Water-incubated control (in microM/min/mg), assay measured in aqueous phase | Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation Activity after incubation (6 hours at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Chloroform 50% 8.1 0.0 Β΅M/min/mg Incubation: ?, Assay: 40 mM phosphate buffer Incubation: ?, Assay: 7 Incubation: Room temperature, Assay: 40Β°C 0.125% CMC (Carboxymethylcellulose sodium) Reducing sugars β€” Incubation: 150 rpm Water-incubated control (in microM/min/mg), assay measured in aqueous phase | Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation Activity after incubation (12 hours at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Chloroform 50% 8.3 0.0 Β΅M/min/mg Incubation: ?, Assay: 40 mM phosphate buffer Incubation: ?, Assay: 7 Incubation: Room temperature, Assay: 40Β°C 0.125% CMC (Carboxymethylcellulose sodium) Reducing sugars β€” Incubation: 150 rpm Water-incubated control (in microM/min/mg), assay measured in aqueous phase | Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation Activity after incubation (18 hours at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Chloroform 50% 8.2 0.0 Β΅M/min/mg Incubation: ?, Assay: 40 mM phosphate buffer Incubation: ?, Assay: 7 Incubation: Room temperature, Assay: 40Β°C 0.125% CMC (Carboxymethylcellulose sodium) Reducing sugars β€” Incubation: 150 rpm Water-incubated control (in microM/min/mg), assay measured in aqueous phase | Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation Activity after incubation (24 hours at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Chloroform 50% 8.0 0.0 Β΅M/min/mg Incubation: ?, Assay: 40 mM phosphate buffer Incubation: ?, Assay: 7 Incubation: Room temperature, Assay: 40Β°C 0.125% CMC (Carboxymethylcellulose sodium) Reducing sugars β€” Incubation: 150 rpm Water-incubated control (in microM/min/mg), assay measured in aqueous phase | Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation Activity after incubation (6 hours at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Toluene 50% 8.1 10.6 Β΅M/min/mg Incubation: ?, Assay: 40 mM phosphate buffer Incubation: ?, Assay: 7 Incubation: Room temperature, Assay: 40Β°C 0.125% CMC (Carboxymethylcellulose sodium) Reducing sugars β€” Incubation: 150 rpm Water-incubated control (in microM/min/mg), assay measured in aqueous phase | Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation Activity after incubation (6 hours at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 50% 8.1 6.8 Β΅M/min/mg Incubation: ?, Assay: 40 mM phosphate buffer Incubation: ?, Assay: 7 Incubation: Room temperature, Assay: 40Β°C 0.125% CMC (Carboxymethylcellulose sodium) Reducing sugars β€” Incubation: 150 rpm Water-incubated control (in microM/min/mg), assay measured in aqueous phase | Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation Activity after incubation (12 hours at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 50% 8.3 7.3 Β΅M/min/mg Incubation: ?, Assay: 40 mM phosphate buffer Incubation: ?, Assay: 7 Incubation: Room temperature, Assay: 40Β°C 0.125% CMC (Carboxymethylcellulose sodium) Reducing sugars β€” Incubation: 150 rpm Water-incubated control (in microM/min/mg), assay measured in aqueous phase | Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation Activity after incubation (18 hours at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 50% 8.2 7.2 Β΅M/min/mg Incubation: ?, Assay: 40 mM phosphate buffer Incubation: ?, Assay: 7 Incubation: Room temperature, Assay: 40Β°C 0.125% CMC (Carboxymethylcellulose sodium) Reducing sugars β€” Incubation: 150 rpm Water-incubated control (in microM/min/mg), assay measured in aqueous phase | Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation Activity after incubation (24 hours at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 50% 8.0 7.5 Β΅M/min/mg Incubation: ?, Assay: 40 mM phosphate buffer Incubation: ?, Assay: 7 Incubation: Room temperature, Assay: 40Β°C 0.125% CMC (Carboxymethylcellulose sodium) Reducing sugars β€” Incubation: 150 rpm Water-incubated control (in microM/min/mg), assay measured in aqueous phase | Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation Activity after incubation (0 hour at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase 1-Butanol 50% 8.4 13.5 Β΅M/min/mg Incubation: ?, Assay: 40 mM phosphate buffer Incubation: ?, Assay: 7 Incubation: Room temperature, Assay: 40Β°C 0.125% CMC (Carboxymethylcellulose sodium) Reducing sugars β€” Incubation: 150 rpm Water-incubated control (in microM/min/mg), assay measured in aqueous phase | Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation Activity after incubation (6 hours at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase 1-Butanol 50% 8.1 13.7 Β΅M/min/mg Incubation: ?, Assay: 40 mM phosphate buffer Incubation: ?, Assay: 7 Incubation: Room temperature, Assay: 40Β°C 0.125% CMC (Carboxymethylcellulose sodium) Reducing sugars β€” Incubation: 150 rpm Water-incubated control (in microM/min/mg), assay measured in aqueous phase | Uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
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Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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