Xylanase from Geobacillus thermodenitrificans A333
Enzyme Description
Extremophile
Yes
thermophilic organism
EC Number
Sequence
MLKRSRKAIIVGFSFMLLLPLGMTNALAKTEQSYAKKPQISALHAPQLDQRYKDSFTIGAAVEPYQLLNEKDAQMLKRHFNSIVAENVMKPINIQPEEGKFNFAEADQIVRFAKKHHMDIRFHTLVWHSQVPQWFFLDKEGQPMVNETDPVKREQNKQLLLKRIETHIKTIVERYKDDIKYWDVVNEVVGDDGELRDSPWYQIAGIDYIKVAFQTARKYGGNKIKLYINDYNTEVEPKRSALYNLVKQLKEEGIPIDGIGHQSHIQIDWPSEEEIEKTIIMFADLGLDNQITELDVSMYGWPPRAYLSYDAIPEQKFLDQADRYDRLFKLYEKLSDKISNVTFWGIADNHTWLDSRADVYYDTDGNVIVDPKAPYTRVEKGNGKDAPFVFDPEYNVKPAYWAIIDHK
Loredana Marcolongo et al. (2015)
β Properties of an alkali-thermo stable xylanase from Geobacillus thermodenitrificans A333 and applicability in xylooligosaccharides generation
World Journal of Microbiology and Biotechnology
Β· doi:10.1007/s11274-015-1818-1 β
Β· Stability - Incubation
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Database ID
UniProt: A4IP71 β
Sequence Annotation
Inferred - from protein name
Protein Source
Purified, bacterium Geobacillus thermodenitrificans A333
Experimental Data (41 measurements)
41 measurements β page 1 of 3
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Stability - Incubation | Activity after incubation (120 minutes at 25Β°C), measured by absorbance spectrophotometry (colorimetric SomogyiβNelson assay, reducing sugars and Nelson's reagant reaction product absorbance measurement, 590 nm) in aqueous phase | Formaldehyde | 15% | 100 | 29 | % | Incubation: ?, Assay: 100 mM sodium phosphate buffer | Incubation: ?, Assay: 7.5 | Incubation: 25Β°C, Assay: 50Β°C | 0.5% Soluble birchwood xylan | Reducing sugars | β | Yes, "under constant agitation" incubation | Water-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions (" The xylanase activity towards beechwood xylan was assessed utilizing aliquots of the incubated enzyme in a reaction mixture with a solvent concentration less than 1 % (v/v).")/sequence is the one with 99% sequence identity to real one |
| Stability - Incubation | Activity after incubation (60 minutes at 25Β°C), measured by absorbance spectrophotometry (colorimetric SomogyiβNelson assay, reducing sugars and Nelson's reagant reaction product absorbance measurement, 590 nm) in aqueous phase | Glycerol | 15% | 100 | 46 | % | Incubation: ?, Assay: 100 mM sodium phosphate buffer | Incubation: ?, Assay: 7.5 | Incubation: 25Β°C, Assay: 50Β°C | 0.5% Soluble birchwood xylan | Reducing sugars | β | Yes, "under constant agitation" incubation | Water-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions (" The xylanase activity towards beechwood xylan was assessed utilizing aliquots of the incubated enzyme in a reaction mixture with a solvent concentration less than 1 % (v/v).")/sequence is the one with 99% sequence identity to real one |
| Stability - Incubation | Activity after incubation (120 minutes at 25Β°C), measured by absorbance spectrophotometry (colorimetric SomogyiβNelson assay, reducing sugars and Nelson's reagant reaction product absorbance measurement, 590 nm) in aqueous phase | Ethanol | 15% | 100 | 96 | % | Incubation: ?, Assay: 100 mM sodium phosphate buffer | Incubation: ?, Assay: 7.5 | Incubation: 25Β°C, Assay: 50Β°C | 0.5% Soluble birchwood xylan | Reducing sugars | β | Yes, "under constant agitation" incubation | Water-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions (" The xylanase activity towards beechwood xylan was assessed utilizing aliquots of the incubated enzyme in a reaction mixture with a solvent concentration less than 1 % (v/v).")/sequence is the one with 99% sequence identity to real one |
| Stability - Incubation | Activity after incubation (120 minutes at 25Β°C), measured by absorbance spectrophotometry (colorimetric SomogyiβNelson assay, reducing sugars and Nelson's reagant reaction product absorbance measurement, 590 nm) in aqueous phase | Methanol | 15% | 100 | 99 | % | Incubation: ?, Assay: 100 mM sodium phosphate buffer | Incubation: ?, Assay: 7.5 | Incubation: 25Β°C, Assay: 50Β°C | 0.5% Soluble birchwood xylan | Reducing sugars | β | Yes, "under constant agitation" incubation | Water-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions (" The xylanase activity towards beechwood xylan was assessed utilizing aliquots of the incubated enzyme in a reaction mixture with a solvent concentration less than 1 % (v/v).")/sequence is the one with 99% sequence identity to real one |
| Stability - Incubation | Activity after incubation (120 minutes at 25Β°C), measured by absorbance spectrophotometry (colorimetric SomogyiβNelson assay, reducing sugars and Nelson's reagant reaction product absorbance measurement, 590 nm) in aqueous phase | Acetone | 15% | 100 | 97 | % | Incubation: ?, Assay: 100 mM sodium phosphate buffer | Incubation: ?, Assay: 7.5 | Incubation: 25Β°C, Assay: 50Β°C | 0.5% Soluble birchwood xylan | Reducing sugars | β | Yes, "under constant agitation" incubation | Water-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions (" The xylanase activity towards beechwood xylan was assessed utilizing aliquots of the incubated enzyme in a reaction mixture with a solvent concentration less than 1 % (v/v).")/sequence is the one with 99% sequence identity to real one |
| Stability - Incubation | Activity after incubation (120 minutes at 25Β°C), measured by absorbance spectrophotometry (colorimetric SomogyiβNelson assay, reducing sugars and Nelson's reagant reaction product absorbance measurement, 590 nm) in aqueous phase | Acetonitrile | 15% | 100 | 87 | % | Incubation: ?, Assay: 100 mM sodium phosphate buffer | Incubation: ?, Assay: 7.5 | Incubation: 25Β°C, Assay: 50Β°C | 0.5% Soluble birchwood xylan | Reducing sugars | β | Yes, "under constant agitation" incubation | Water-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions (" The xylanase activity towards beechwood xylan was assessed utilizing aliquots of the incubated enzyme in a reaction mixture with a solvent concentration less than 1 % (v/v).")/sequence is the one with 99% sequence identity to real one |
| Stability - Incubation | Activity after incubation (120 minutes at 25Β°C), measured by absorbance spectrophotometry (colorimetric SomogyiβNelson assay, reducing sugars and Nelson's reagant reaction product absorbance measurement, 590 nm) in aqueous phase | Ethyl Acetate | 15% | 100 | 98 | % | Incubation: ?, Assay: 100 mM sodium phosphate buffer | Incubation: ?, Assay: 7.5 | Incubation: 25Β°C, Assay: 50Β°C | 0.5% Soluble birchwood xylan | Reducing sugars | β | Yes, "under constant agitation" incubation | Water-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions (" The xylanase activity towards beechwood xylan was assessed utilizing aliquots of the incubated enzyme in a reaction mixture with a solvent concentration less than 1 % (v/v).")/sequence is the one with 99% sequence identity to real one |
| Stability - Incubation | Activity after incubation (120 minutes at 25Β°C), measured by absorbance spectrophotometry (colorimetric SomogyiβNelson assay, reducing sugars and Nelson's reagant reaction product absorbance measurement, 590 nm) in aqueous phase | Isopropanol | 15% | 100 | 92 | % | Incubation: ?, Assay: 100 mM sodium phosphate buffer | Incubation: ?, Assay: 7.5 | Incubation: 25Β°C, Assay: 50Β°C | 0.5% Soluble birchwood xylan | Reducing sugars | β | Yes, "under constant agitation" incubation | Water-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions (" The xylanase activity towards beechwood xylan was assessed utilizing aliquots of the incubated enzyme in a reaction mixture with a solvent concentration less than 1 % (v/v).")/sequence is the one with 99% sequence identity to real one |
| Stability - Incubation | Activity after incubation (120 minutes at 25Β°C), measured by absorbance spectrophotometry (colorimetric SomogyiβNelson assay, reducing sugars and Nelson's reagant reaction product absorbance measurement, 590 nm) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 15% | 100 | 85 | % | Incubation: ?, Assay: 100 mM sodium phosphate buffer | Incubation: ?, Assay: 7.5 | Incubation: 25Β°C, Assay: 50Β°C | 0.5% Soluble birchwood xylan | Reducing sugars | β | Yes, "under constant agitation" incubation | Water-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions (" The xylanase activity towards beechwood xylan was assessed utilizing aliquots of the incubated enzyme in a reaction mixture with a solvent concentration less than 1 % (v/v).")/sequence is the one with 99% sequence identity to real one |
| Stability - Incubation | Activity after incubation (120 minutes at 25Β°C), measured by absorbance spectrophotometry (colorimetric SomogyiβNelson assay, reducing sugars and Nelson's reagant reaction product absorbance measurement, 590 nm) in aqueous phase | Dimethylformamide (DMF) | 15% | 100 | 99 | % | Incubation: ?, Assay: 100 mM sodium phosphate buffer | Incubation: ?, Assay: 7.5 | Incubation: 25Β°C, Assay: 50Β°C | 0.5% Soluble birchwood xylan | Reducing sugars | β | Yes, "under constant agitation" incubation | Water-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions (" The xylanase activity towards beechwood xylan was assessed utilizing aliquots of the incubated enzyme in a reaction mixture with a solvent concentration less than 1 % (v/v).")/sequence is the one with 99% sequence identity to real one |
| Stability - Incubation | Activity after incubation (120 minutes at 25Β°C), measured by absorbance spectrophotometry (colorimetric SomogyiβNelson assay, reducing sugars and Nelson's reagant reaction product absorbance measurement, 590 nm) in aqueous phase | 1-Butanol | 15% | 100 | 114 | % | Incubation: ?, Assay: 100 mM sodium phosphate buffer | Incubation: ?, Assay: 7.5 | Incubation: 25Β°C, Assay: 50Β°C | 0.5% Soluble birchwood xylan | Reducing sugars | β | Yes, "under constant agitation" incubation | Water-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions (" The xylanase activity towards beechwood xylan was assessed utilizing aliquots of the incubated enzyme in a reaction mixture with a solvent concentration less than 1 % (v/v).")/sequence is the one with 99% sequence identity to real one |
| Stability - Incubation | Activity after incubation (60 minutes at 25Β°C), measured by absorbance spectrophotometry (colorimetric SomogyiβNelson assay, reducing sugars and Nelson's reagant reaction product absorbance measurement, 590 nm) in aqueous phase | Formaldehyde | 15% | 100 | 42 | % | Incubation: ?, Assay: 100 mM sodium phosphate buffer | Incubation: ?, Assay: 7.5 | Incubation: 25Β°C, Assay: 50Β°C | 0.5% Soluble birchwood xylan | Reducing sugars | β | Yes, "under constant agitation" incubation | Water-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions (" The xylanase activity towards beechwood xylan was assessed utilizing aliquots of the incubated enzyme in a reaction mixture with a solvent concentration less than 1 % (v/v).")/sequence is the one with 99% sequence identity to real one |
| Stability - Incubation | Activity after incubation (120 minutes at 25Β°C), measured by absorbance spectrophotometry (colorimetric SomogyiβNelson assay, reducing sugars and Nelson's reagant reaction product absorbance measurement, 590 nm) in aqueous phase | Glycerol | 15% | 100 | 38 | % | Incubation: ?, Assay: 100 mM sodium phosphate buffer | Incubation: ?, Assay: 7.5 | Incubation: 25Β°C, Assay: 50Β°C | 0.5% Soluble birchwood xylan | Reducing sugars | β | Yes, "under constant agitation" incubation | Water-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions (" The xylanase activity towards beechwood xylan was assessed utilizing aliquots of the incubated enzyme in a reaction mixture with a solvent concentration less than 1 % (v/v).")/sequence is the one with 99% sequence identity to real one |
| Stability - Incubation | Activity after incubation (120 minutes at 25Β°C), measured by absorbance spectrophotometry (colorimetric SomogyiβNelson assay, reducing sugars and Nelson's reagant reaction product absorbance measurement, 590 nm) in aqueous phase | Ethanol | 10% | 100 | 107 | % | Incubation: ?, Assay: 100 mM sodium phosphate buffer | Incubation: ?, Assay: 7.5 | Incubation: 25Β°C, Assay: 50Β°C | 0.5% Soluble birchwood xylan | Reducing sugars | β | Yes, "under constant agitation" incubation | Water-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions (" The xylanase activity towards beechwood xylan was assessed utilizing aliquots of the incubated enzyme in a reaction mixture with a solvent concentration less than 1 % (v/v).")/sequence is the one with 99% sequence identity to real one |
| Stability - Incubation | Activity after incubation (120 minutes at 25Β°C), measured by absorbance spectrophotometry (colorimetric SomogyiβNelson assay, reducing sugars and Nelson's reagant reaction product absorbance measurement, 590 nm) in aqueous phase | Ethanol | 20% | 100 | 112 | % | Incubation: ?, Assay: 100 mM sodium phosphate buffer | Incubation: ?, Assay: 7.5 | Incubation: 25Β°C, Assay: 50Β°C | 0.5% Soluble birchwood xylan | Reducing sugars | β | Yes, "under constant agitation" incubation | Water-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions (" The xylanase activity towards beechwood xylan was assessed utilizing aliquots of the incubated enzyme in a reaction mixture with a solvent concentration less than 1 % (v/v).")/sequence is the one with 99% sequence identity to real one |
| Stability - Incubation | Activity after incubation (120 minutes at 25Β°C), measured by absorbance spectrophotometry (colorimetric SomogyiβNelson assay, reducing sugars and Nelson's reagant reaction product absorbance measurement, 590 nm) in aqueous phase | Ethanol | 30% | 100 | 100 | % | Incubation: ?, Assay: 100 mM sodium phosphate buffer | Incubation: ?, Assay: 7.5 | Incubation: 25Β°C, Assay: 50Β°C | 0.5% Soluble birchwood xylan | Reducing sugars | β | Yes, "under constant agitation" incubation | Water-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions (" The xylanase activity towards beechwood xylan was assessed utilizing aliquots of the incubated enzyme in a reaction mixture with a solvent concentration less than 1 % (v/v).")/sequence is the one with 99% sequence identity to real one |
| Stability - Incubation | Activity after incubation (120 minutes at 25Β°C), measured by absorbance spectrophotometry (colorimetric SomogyiβNelson assay, reducing sugars and Nelson's reagant reaction product absorbance measurement, 590 nm) in aqueous phase | Ethanol | 40% | 100 | 99 | % | Incubation: ?, Assay: 100 mM sodium phosphate buffer | Incubation: ?, Assay: 7.5 | Incubation: 25Β°C, Assay: 50Β°C | 0.5% Soluble birchwood xylan | Reducing sugars | β | Yes, "under constant agitation" incubation | Water-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions (" The xylanase activity towards beechwood xylan was assessed utilizing aliquots of the incubated enzyme in a reaction mixture with a solvent concentration less than 1 % (v/v).")/sequence is the one with 99% sequence identity to real one |
| Stability - Incubation | Activity after incubation (120 minutes at 25Β°C), measured by absorbance spectrophotometry (colorimetric SomogyiβNelson assay, reducing sugars and Nelson's reagant reaction product absorbance measurement, 590 nm) in aqueous phase | Ethanol | 50% | 100 | 98 | % | Incubation: ?, Assay: 100 mM sodium phosphate buffer | Incubation: ?, Assay: 7.5 | Incubation: 25Β°C, Assay: 50Β°C | 0.5% Soluble birchwood xylan | Reducing sugars | β | Yes, "under constant agitation" incubation | Water-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions (" The xylanase activity towards beechwood xylan was assessed utilizing aliquots of the incubated enzyme in a reaction mixture with a solvent concentration less than 1 % (v/v).")/sequence is the one with 99% sequence identity to real one |
| Stability - Incubation | Activity after incubation (120 minutes at 25Β°C), measured by absorbance spectrophotometry (colorimetric SomogyiβNelson assay, reducing sugars and Nelson's reagant reaction product absorbance measurement, 590 nm) in aqueous phase | Ethanol | 60% | 100 | 77 | % | Incubation: ?, Assay: 100 mM sodium phosphate buffer | Incubation: ?, Assay: 7.5 | Incubation: 25Β°C, Assay: 50Β°C | 0.5% Soluble birchwood xylan | Reducing sugars | β | Yes, "under constant agitation" incubation | Water-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions (" The xylanase activity towards beechwood xylan was assessed utilizing aliquots of the incubated enzyme in a reaction mixture with a solvent concentration less than 1 % (v/v).")/sequence is the one with 99% sequence identity to real one |
| Stability - Incubation | Activity after incubation (120 minutes at 25Β°C), measured by absorbance spectrophotometry (colorimetric SomogyiβNelson assay, reducing sugars and Nelson's reagant reaction product absorbance measurement, 590 nm) in aqueous phase | Ethanol | 70% | 100 | 74 | % | Incubation: ?, Assay: 100 mM sodium phosphate buffer | Incubation: ?, Assay: 7.5 | Incubation: 25Β°C, Assay: 50Β°C | 0.5% Soluble birchwood xylan | Reducing sugars | β | Yes, "under constant agitation" incubation | Water-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions (" The xylanase activity towards beechwood xylan was assessed utilizing aliquots of the incubated enzyme in a reaction mixture with a solvent concentration less than 1 % (v/v).")/sequence is the one with 99% sequence identity to real one |
Visualization : Stability β Incubation
One bar per measurement. Colour = solvent, shade = solvent volume.