| Stability - Incubation |
Activity after incubation (120 minutes at 25Β°C), measured by absorbance spectrophotometry (colorimetric SomogyiβNelson assay, reducing sugars and Nelson's reagant reaction product absorbance measurement, 590 nm) in aqueous phase |
Formaldehyde |
15% |
100 |
29 |
% |
Incubation: ?, Assay: 100 mM sodium phosphate buffer |
Incubation: ?, Assay: 7.5 |
Incubation: 25Β°C, Assay: 50Β°C |
0.5% Soluble birchwood xylan |
Reducing sugars |
β |
Yes, "under constant agitation" incubation |
Water-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions (" The xylanase activity towards beechwood xylan was assessed utilizing aliquots of the incubated enzyme in a reaction mixture with a solvent concentration less than 1 % (v/v).")/sequence is the one with 99% sequence identity to real one |
| Stability - Incubation |
Activity after incubation (60 minutes at 25Β°C), measured by absorbance spectrophotometry (colorimetric SomogyiβNelson assay, reducing sugars and Nelson's reagant reaction product absorbance measurement, 590 nm) in aqueous phase |
Glycerol |
15% |
100 |
46 |
% |
Incubation: ?, Assay: 100 mM sodium phosphate buffer |
Incubation: ?, Assay: 7.5 |
Incubation: 25Β°C, Assay: 50Β°C |
0.5% Soluble birchwood xylan |
Reducing sugars |
β |
Yes, "under constant agitation" incubation |
Water-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions (" The xylanase activity towards beechwood xylan was assessed utilizing aliquots of the incubated enzyme in a reaction mixture with a solvent concentration less than 1 % (v/v).")/sequence is the one with 99% sequence identity to real one |
| Stability - Incubation |
Activity after incubation (120 minutes at 25Β°C), measured by absorbance spectrophotometry (colorimetric SomogyiβNelson assay, reducing sugars and Nelson's reagant reaction product absorbance measurement, 590 nm) in aqueous phase |
Ethanol |
15% |
100 |
96 |
% |
Incubation: ?, Assay: 100 mM sodium phosphate buffer |
Incubation: ?, Assay: 7.5 |
Incubation: 25Β°C, Assay: 50Β°C |
0.5% Soluble birchwood xylan |
Reducing sugars |
β |
Yes, "under constant agitation" incubation |
Water-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions (" The xylanase activity towards beechwood xylan was assessed utilizing aliquots of the incubated enzyme in a reaction mixture with a solvent concentration less than 1 % (v/v).")/sequence is the one with 99% sequence identity to real one |
| Stability - Incubation |
Activity after incubation (120 minutes at 25Β°C), measured by absorbance spectrophotometry (colorimetric SomogyiβNelson assay, reducing sugars and Nelson's reagant reaction product absorbance measurement, 590 nm) in aqueous phase |
Methanol |
15% |
100 |
99 |
% |
Incubation: ?, Assay: 100 mM sodium phosphate buffer |
Incubation: ?, Assay: 7.5 |
Incubation: 25Β°C, Assay: 50Β°C |
0.5% Soluble birchwood xylan |
Reducing sugars |
β |
Yes, "under constant agitation" incubation |
Water-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions (" The xylanase activity towards beechwood xylan was assessed utilizing aliquots of the incubated enzyme in a reaction mixture with a solvent concentration less than 1 % (v/v).")/sequence is the one with 99% sequence identity to real one |
| Stability - Incubation |
Activity after incubation (120 minutes at 25Β°C), measured by absorbance spectrophotometry (colorimetric SomogyiβNelson assay, reducing sugars and Nelson's reagant reaction product absorbance measurement, 590 nm) in aqueous phase |
Acetone |
15% |
100 |
97 |
% |
Incubation: ?, Assay: 100 mM sodium phosphate buffer |
Incubation: ?, Assay: 7.5 |
Incubation: 25Β°C, Assay: 50Β°C |
0.5% Soluble birchwood xylan |
Reducing sugars |
β |
Yes, "under constant agitation" incubation |
Water-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions (" The xylanase activity towards beechwood xylan was assessed utilizing aliquots of the incubated enzyme in a reaction mixture with a solvent concentration less than 1 % (v/v).")/sequence is the one with 99% sequence identity to real one |
| Stability - Incubation |
Activity after incubation (120 minutes at 25Β°C), measured by absorbance spectrophotometry (colorimetric SomogyiβNelson assay, reducing sugars and Nelson's reagant reaction product absorbance measurement, 590 nm) in aqueous phase |
Acetonitrile |
15% |
100 |
87 |
% |
Incubation: ?, Assay: 100 mM sodium phosphate buffer |
Incubation: ?, Assay: 7.5 |
Incubation: 25Β°C, Assay: 50Β°C |
0.5% Soluble birchwood xylan |
Reducing sugars |
β |
Yes, "under constant agitation" incubation |
Water-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions (" The xylanase activity towards beechwood xylan was assessed utilizing aliquots of the incubated enzyme in a reaction mixture with a solvent concentration less than 1 % (v/v).")/sequence is the one with 99% sequence identity to real one |
| Stability - Incubation |
Activity after incubation (120 minutes at 25Β°C), measured by absorbance spectrophotometry (colorimetric SomogyiβNelson assay, reducing sugars and Nelson's reagant reaction product absorbance measurement, 590 nm) in aqueous phase |
Ethyl Acetate |
15% |
100 |
98 |
% |
Incubation: ?, Assay: 100 mM sodium phosphate buffer |
Incubation: ?, Assay: 7.5 |
Incubation: 25Β°C, Assay: 50Β°C |
0.5% Soluble birchwood xylan |
Reducing sugars |
β |
Yes, "under constant agitation" incubation |
Water-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions (" The xylanase activity towards beechwood xylan was assessed utilizing aliquots of the incubated enzyme in a reaction mixture with a solvent concentration less than 1 % (v/v).")/sequence is the one with 99% sequence identity to real one |
| Stability - Incubation |
Activity after incubation (120 minutes at 25Β°C), measured by absorbance spectrophotometry (colorimetric SomogyiβNelson assay, reducing sugars and Nelson's reagant reaction product absorbance measurement, 590 nm) in aqueous phase |
Isopropanol |
15% |
100 |
92 |
% |
Incubation: ?, Assay: 100 mM sodium phosphate buffer |
Incubation: ?, Assay: 7.5 |
Incubation: 25Β°C, Assay: 50Β°C |
0.5% Soluble birchwood xylan |
Reducing sugars |
β |
Yes, "under constant agitation" incubation |
Water-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions (" The xylanase activity towards beechwood xylan was assessed utilizing aliquots of the incubated enzyme in a reaction mixture with a solvent concentration less than 1 % (v/v).")/sequence is the one with 99% sequence identity to real one |
| Stability - Incubation |
Activity after incubation (120 minutes at 25Β°C), measured by absorbance spectrophotometry (colorimetric SomogyiβNelson assay, reducing sugars and Nelson's reagant reaction product absorbance measurement, 590 nm) in aqueous phase |
Dimethyl Sulfoxide (DMSO) |
15% |
100 |
85 |
% |
Incubation: ?, Assay: 100 mM sodium phosphate buffer |
Incubation: ?, Assay: 7.5 |
Incubation: 25Β°C, Assay: 50Β°C |
0.5% Soluble birchwood xylan |
Reducing sugars |
β |
Yes, "under constant agitation" incubation |
Water-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions (" The xylanase activity towards beechwood xylan was assessed utilizing aliquots of the incubated enzyme in a reaction mixture with a solvent concentration less than 1 % (v/v).")/sequence is the one with 99% sequence identity to real one |
| Stability - Incubation |
Activity after incubation (120 minutes at 25Β°C), measured by absorbance spectrophotometry (colorimetric SomogyiβNelson assay, reducing sugars and Nelson's reagant reaction product absorbance measurement, 590 nm) in aqueous phase |
Dimethylformamide (DMF) |
15% |
100 |
99 |
% |
Incubation: ?, Assay: 100 mM sodium phosphate buffer |
Incubation: ?, Assay: 7.5 |
Incubation: 25Β°C, Assay: 50Β°C |
0.5% Soluble birchwood xylan |
Reducing sugars |
β |
Yes, "under constant agitation" incubation |
Water-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions (" The xylanase activity towards beechwood xylan was assessed utilizing aliquots of the incubated enzyme in a reaction mixture with a solvent concentration less than 1 % (v/v).")/sequence is the one with 99% sequence identity to real one |
| Stability - Incubation |
Activity after incubation (120 minutes at 25Β°C), measured by absorbance spectrophotometry (colorimetric SomogyiβNelson assay, reducing sugars and Nelson's reagant reaction product absorbance measurement, 590 nm) in aqueous phase |
1-Butanol |
15% |
100 |
114 |
% |
Incubation: ?, Assay: 100 mM sodium phosphate buffer |
Incubation: ?, Assay: 7.5 |
Incubation: 25Β°C, Assay: 50Β°C |
0.5% Soluble birchwood xylan |
Reducing sugars |
β |
Yes, "under constant agitation" incubation |
Water-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions (" The xylanase activity towards beechwood xylan was assessed utilizing aliquots of the incubated enzyme in a reaction mixture with a solvent concentration less than 1 % (v/v).")/sequence is the one with 99% sequence identity to real one |
| Stability - Incubation |
Activity after incubation (60 minutes at 25Β°C), measured by absorbance spectrophotometry (colorimetric SomogyiβNelson assay, reducing sugars and Nelson's reagant reaction product absorbance measurement, 590 nm) in aqueous phase |
Formaldehyde |
15% |
100 |
42 |
% |
Incubation: ?, Assay: 100 mM sodium phosphate buffer |
Incubation: ?, Assay: 7.5 |
Incubation: 25Β°C, Assay: 50Β°C |
0.5% Soluble birchwood xylan |
Reducing sugars |
β |
Yes, "under constant agitation" incubation |
Water-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions (" The xylanase activity towards beechwood xylan was assessed utilizing aliquots of the incubated enzyme in a reaction mixture with a solvent concentration less than 1 % (v/v).")/sequence is the one with 99% sequence identity to real one |
| Stability - Incubation |
Activity after incubation (120 minutes at 25Β°C), measured by absorbance spectrophotometry (colorimetric SomogyiβNelson assay, reducing sugars and Nelson's reagant reaction product absorbance measurement, 590 nm) in aqueous phase |
Glycerol |
15% |
100 |
38 |
% |
Incubation: ?, Assay: 100 mM sodium phosphate buffer |
Incubation: ?, Assay: 7.5 |
Incubation: 25Β°C, Assay: 50Β°C |
0.5% Soluble birchwood xylan |
Reducing sugars |
β |
Yes, "under constant agitation" incubation |
Water-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions (" The xylanase activity towards beechwood xylan was assessed utilizing aliquots of the incubated enzyme in a reaction mixture with a solvent concentration less than 1 % (v/v).")/sequence is the one with 99% sequence identity to real one |
| Stability - Incubation |
Activity after incubation (120 minutes at 25Β°C), measured by absorbance spectrophotometry (colorimetric SomogyiβNelson assay, reducing sugars and Nelson's reagant reaction product absorbance measurement, 590 nm) in aqueous phase |
Ethanol |
10% |
100 |
107 |
% |
Incubation: ?, Assay: 100 mM sodium phosphate buffer |
Incubation: ?, Assay: 7.5 |
Incubation: 25Β°C, Assay: 50Β°C |
0.5% Soluble birchwood xylan |
Reducing sugars |
β |
Yes, "under constant agitation" incubation |
Water-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions (" The xylanase activity towards beechwood xylan was assessed utilizing aliquots of the incubated enzyme in a reaction mixture with a solvent concentration less than 1 % (v/v).")/sequence is the one with 99% sequence identity to real one |
| Stability - Incubation |
Activity after incubation (120 minutes at 25Β°C), measured by absorbance spectrophotometry (colorimetric SomogyiβNelson assay, reducing sugars and Nelson's reagant reaction product absorbance measurement, 590 nm) in aqueous phase |
Ethanol |
20% |
100 |
112 |
% |
Incubation: ?, Assay: 100 mM sodium phosphate buffer |
Incubation: ?, Assay: 7.5 |
Incubation: 25Β°C, Assay: 50Β°C |
0.5% Soluble birchwood xylan |
Reducing sugars |
β |
Yes, "under constant agitation" incubation |
Water-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions (" The xylanase activity towards beechwood xylan was assessed utilizing aliquots of the incubated enzyme in a reaction mixture with a solvent concentration less than 1 % (v/v).")/sequence is the one with 99% sequence identity to real one |
| Stability - Incubation |
Activity after incubation (120 minutes at 25Β°C), measured by absorbance spectrophotometry (colorimetric SomogyiβNelson assay, reducing sugars and Nelson's reagant reaction product absorbance measurement, 590 nm) in aqueous phase |
Ethanol |
30% |
100 |
100 |
% |
Incubation: ?, Assay: 100 mM sodium phosphate buffer |
Incubation: ?, Assay: 7.5 |
Incubation: 25Β°C, Assay: 50Β°C |
0.5% Soluble birchwood xylan |
Reducing sugars |
β |
Yes, "under constant agitation" incubation |
Water-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions (" The xylanase activity towards beechwood xylan was assessed utilizing aliquots of the incubated enzyme in a reaction mixture with a solvent concentration less than 1 % (v/v).")/sequence is the one with 99% sequence identity to real one |
| Stability - Incubation |
Activity after incubation (120 minutes at 25Β°C), measured by absorbance spectrophotometry (colorimetric SomogyiβNelson assay, reducing sugars and Nelson's reagant reaction product absorbance measurement, 590 nm) in aqueous phase |
Ethanol |
40% |
100 |
99 |
% |
Incubation: ?, Assay: 100 mM sodium phosphate buffer |
Incubation: ?, Assay: 7.5 |
Incubation: 25Β°C, Assay: 50Β°C |
0.5% Soluble birchwood xylan |
Reducing sugars |
β |
Yes, "under constant agitation" incubation |
Water-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions (" The xylanase activity towards beechwood xylan was assessed utilizing aliquots of the incubated enzyme in a reaction mixture with a solvent concentration less than 1 % (v/v).")/sequence is the one with 99% sequence identity to real one |
| Stability - Incubation |
Activity after incubation (120 minutes at 25Β°C), measured by absorbance spectrophotometry (colorimetric SomogyiβNelson assay, reducing sugars and Nelson's reagant reaction product absorbance measurement, 590 nm) in aqueous phase |
Ethanol |
50% |
100 |
98 |
% |
Incubation: ?, Assay: 100 mM sodium phosphate buffer |
Incubation: ?, Assay: 7.5 |
Incubation: 25Β°C, Assay: 50Β°C |
0.5% Soluble birchwood xylan |
Reducing sugars |
β |
Yes, "under constant agitation" incubation |
Water-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions (" The xylanase activity towards beechwood xylan was assessed utilizing aliquots of the incubated enzyme in a reaction mixture with a solvent concentration less than 1 % (v/v).")/sequence is the one with 99% sequence identity to real one |
| Stability - Incubation |
Activity after incubation (120 minutes at 25Β°C), measured by absorbance spectrophotometry (colorimetric SomogyiβNelson assay, reducing sugars and Nelson's reagant reaction product absorbance measurement, 590 nm) in aqueous phase |
Ethanol |
60% |
100 |
77 |
% |
Incubation: ?, Assay: 100 mM sodium phosphate buffer |
Incubation: ?, Assay: 7.5 |
Incubation: 25Β°C, Assay: 50Β°C |
0.5% Soluble birchwood xylan |
Reducing sugars |
β |
Yes, "under constant agitation" incubation |
Water-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions (" The xylanase activity towards beechwood xylan was assessed utilizing aliquots of the incubated enzyme in a reaction mixture with a solvent concentration less than 1 % (v/v).")/sequence is the one with 99% sequence identity to real one |
| Stability - Incubation |
Activity after incubation (120 minutes at 25Β°C), measured by absorbance spectrophotometry (colorimetric SomogyiβNelson assay, reducing sugars and Nelson's reagant reaction product absorbance measurement, 590 nm) in aqueous phase |
Ethanol |
70% |
100 |
74 |
% |
Incubation: ?, Assay: 100 mM sodium phosphate buffer |
Incubation: ?, Assay: 7.5 |
Incubation: 25Β°C, Assay: 50Β°C |
0.5% Soluble birchwood xylan |
Reducing sugars |
β |
Yes, "under constant agitation" incubation |
Water-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions (" The xylanase activity towards beechwood xylan was assessed utilizing aliquots of the incubated enzyme in a reaction mixture with a solvent concentration less than 1 % (v/v).")/sequence is the one with 99% sequence identity to real one |
| Stability - Incubation |
Activity after incubation (120 minutes at 25Β°C), measured by absorbance spectrophotometry (colorimetric SomogyiβNelson assay, reducing sugars and Nelson's reagant reaction product absorbance measurement, 590 nm) in aqueous phase |
Ethanol |
80% |
100 |
66 |
% |
Incubation: ?, Assay: 100 mM sodium phosphate buffer |
Incubation: ?, Assay: 7.5 |
Incubation: 25Β°C, Assay: 50Β°C |
0.5% Soluble birchwood xylan |
Reducing sugars |
β |
Yes, "under constant agitation" incubation |
Water-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions (" The xylanase activity towards beechwood xylan was assessed utilizing aliquots of the incubated enzyme in a reaction mixture with a solvent concentration less than 1 % (v/v).")/sequence is the one with 99% sequence identity to real one |
| Stability - Incubation |
Activity after incubation (30 minutes at 25Β°C), measured by absorbance spectrophotometry (colorimetric SomogyiβNelson assay, reducing sugars and Nelson's reagant reaction product absorbance measurement, 590 nm) in aqueous phase |
Glycerol |
15% |
100 |
58 |
% |
Incubation: ?, Assay: 100 mM sodium phosphate buffer |
Incubation: ?, Assay: 7.5 |
Incubation: 25Β°C, Assay: 50Β°C |
0.5% Soluble birchwood xylan |
Reducing sugars |
β |
Yes, "under constant agitation" incubation |
Water-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions (" The xylanase activity towards beechwood xylan was assessed utilizing aliquots of the incubated enzyme in a reaction mixture with a solvent concentration less than 1 % (v/v).")/sequence is the one with 99% sequence identity to real one |
| Stability - Incubation |
Activity after incubation (30 minutes at 25Β°C), measured by absorbance spectrophotometry (colorimetric SomogyiβNelson assay, reducing sugars and Nelson's reagant reaction product absorbance measurement, 590 nm) in aqueous phase |
Methanol |
15% |
100 |
100 |
% |
Incubation: ?, Assay: 100 mM sodium phosphate buffer |
Incubation: ?, Assay: 7.5 |
Incubation: 25Β°C, Assay: 50Β°C |
0.5% Soluble birchwood xylan |
Reducing sugars |
β |
Yes, "under constant agitation" incubation |
Water-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions (" The xylanase activity towards beechwood xylan was assessed utilizing aliquots of the incubated enzyme in a reaction mixture with a solvent concentration less than 1 % (v/v).")/sequence is the one with 99% sequence identity to real one |
| Stability - Incubation |
Activity after incubation (30 minutes at 25Β°C), measured by absorbance spectrophotometry (colorimetric SomogyiβNelson assay, reducing sugars and Nelson's reagant reaction product absorbance measurement, 590 nm) in aqueous phase |
Acetone |
15% |
100 |
92 |
% |
Incubation: ?, Assay: 100 mM sodium phosphate buffer |
Incubation: ?, Assay: 7.5 |
Incubation: 25Β°C, Assay: 50Β°C |
0.5% Soluble birchwood xylan |
Reducing sugars |
β |
Yes, "under constant agitation" incubation |
Water-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions (" The xylanase activity towards beechwood xylan was assessed utilizing aliquots of the incubated enzyme in a reaction mixture with a solvent concentration less than 1 % (v/v).")/sequence is the one with 99% sequence identity to real one |
| Stability - Incubation |
Activity after incubation (30 minutes at 25Β°C), measured by absorbance spectrophotometry (colorimetric SomogyiβNelson assay, reducing sugars and Nelson's reagant reaction product absorbance measurement, 590 nm) in aqueous phase |
Acetonitrile |
15% |
100 |
98 |
% |
Incubation: ?, Assay: 100 mM sodium phosphate buffer |
Incubation: ?, Assay: 7.5 |
Incubation: 25Β°C, Assay: 50Β°C |
0.5% Soluble birchwood xylan |
Reducing sugars |
β |
Yes, "under constant agitation" incubation |
Water-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions (" The xylanase activity towards beechwood xylan was assessed utilizing aliquots of the incubated enzyme in a reaction mixture with a solvent concentration less than 1 % (v/v).")/sequence is the one with 99% sequence identity to real one |
| Stability - Incubation |
Activity after incubation (30 minutes at 25Β°C), measured by absorbance spectrophotometry (colorimetric SomogyiβNelson assay, reducing sugars and Nelson's reagant reaction product absorbance measurement, 590 nm) in aqueous phase |
Ethyl Acetate |
15% |
100 |
100 |
% |
Incubation: ?, Assay: 100 mM sodium phosphate buffer |
Incubation: ?, Assay: 7.5 |
Incubation: 25Β°C, Assay: 50Β°C |
0.5% Soluble birchwood xylan |
Reducing sugars |
β |
Yes, "under constant agitation" incubation |
Water-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions (" The xylanase activity towards beechwood xylan was assessed utilizing aliquots of the incubated enzyme in a reaction mixture with a solvent concentration less than 1 % (v/v).")/sequence is the one with 99% sequence identity to real one |
| Stability - Incubation |
Activity after incubation (30 minutes at 25Β°C), measured by absorbance spectrophotometry (colorimetric SomogyiβNelson assay, reducing sugars and Nelson's reagant reaction product absorbance measurement, 590 nm) in aqueous phase |
Isopropanol |
15% |
100 |
99 |
% |
Incubation: ?, Assay: 100 mM sodium phosphate buffer |
Incubation: ?, Assay: 7.5 |
Incubation: 25Β°C, Assay: 50Β°C |
0.5% Soluble birchwood xylan |
Reducing sugars |
β |
Yes, "under constant agitation" incubation |
Water-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions (" The xylanase activity towards beechwood xylan was assessed utilizing aliquots of the incubated enzyme in a reaction mixture with a solvent concentration less than 1 % (v/v).")/sequence is the one with 99% sequence identity to real one |
| Stability - Incubation |
Activity after incubation (30 minutes at 25Β°C), measured by absorbance spectrophotometry (colorimetric SomogyiβNelson assay, reducing sugars and Nelson's reagant reaction product absorbance measurement, 590 nm) in aqueous phase |
Dimethyl Sulfoxide (DMSO) |
15% |
100 |
96 |
% |
Incubation: ?, Assay: 100 mM sodium phosphate buffer |
Incubation: ?, Assay: 7.5 |
Incubation: 25Β°C, Assay: 50Β°C |
0.5% Soluble birchwood xylan |
Reducing sugars |
β |
Yes, "under constant agitation" incubation |
Water-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions (" The xylanase activity towards beechwood xylan was assessed utilizing aliquots of the incubated enzyme in a reaction mixture with a solvent concentration less than 1 % (v/v).")/sequence is the one with 99% sequence identity to real one |
| Stability - Incubation |
Activity after incubation (30 minutes at 25Β°C), measured by absorbance spectrophotometry (colorimetric SomogyiβNelson assay, reducing sugars and Nelson's reagant reaction product absorbance measurement, 590 nm) in aqueous phase |
Dimethylformamide (DMF) |
15% |
100 |
100 |
% |
Incubation: ?, Assay: 100 mM sodium phosphate buffer |
Incubation: ?, Assay: 7.5 |
Incubation: 25Β°C, Assay: 50Β°C |
0.5% Soluble birchwood xylan |
Reducing sugars |
β |
Yes, "under constant agitation" incubation |
Water-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions (" The xylanase activity towards beechwood xylan was assessed utilizing aliquots of the incubated enzyme in a reaction mixture with a solvent concentration less than 1 % (v/v).")/sequence is the one with 99% sequence identity to real one |
| Stability - Incubation |
Activity after incubation (30 minutes at 25Β°C), measured by absorbance spectrophotometry (colorimetric SomogyiβNelson assay, reducing sugars and Nelson's reagant reaction product absorbance measurement, 590 nm) in aqueous phase |
1-Butanol |
15% |
100 |
100 |
% |
Incubation: ?, Assay: 100 mM sodium phosphate buffer |
Incubation: ?, Assay: 7.5 |
Incubation: 25Β°C, Assay: 50Β°C |
0.5% Soluble birchwood xylan |
Reducing sugars |
β |
Yes, "under constant agitation" incubation |
Water-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions (" The xylanase activity towards beechwood xylan was assessed utilizing aliquots of the incubated enzyme in a reaction mixture with a solvent concentration less than 1 % (v/v).")/sequence is the one with 99% sequence identity to real one |
| Stability - Incubation |
Activity after incubation (30 minutes at 25Β°C), measured by absorbance spectrophotometry (colorimetric SomogyiβNelson assay, reducing sugars and Nelson's reagant reaction product absorbance measurement, 590 nm) in aqueous phase |
Formaldehyde |
15% |
100 |
60 |
% |
Incubation: ?, Assay: 100 mM sodium phosphate buffer |
Incubation: ?, Assay: 7.5 |
Incubation: 25Β°C, Assay: 50Β°C |
0.5% Soluble birchwood xylan |
Reducing sugars |
β |
Yes, "under constant agitation" incubation |
Water-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions (" The xylanase activity towards beechwood xylan was assessed utilizing aliquots of the incubated enzyme in a reaction mixture with a solvent concentration less than 1 % (v/v).")/sequence is the one with 99% sequence identity to real one |
| Stability - Incubation |
Activity after incubation (30 minutes at 25Β°C), measured by absorbance spectrophotometry (colorimetric SomogyiβNelson assay, reducing sugars and Nelson's reagant reaction product absorbance measurement, 590 nm) in aqueous phase |
Ethanol |
15% |
100 |
100 |
% |
Incubation: ?, Assay: 100 mM sodium phosphate buffer |
Incubation: ?, Assay: 7.5 |
Incubation: 25Β°C, Assay: 50Β°C |
0.5% Soluble birchwood xylan |
Reducing sugars |
β |
Yes, "under constant agitation" incubation |
Water-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions (" The xylanase activity towards beechwood xylan was assessed utilizing aliquots of the incubated enzyme in a reaction mixture with a solvent concentration less than 1 % (v/v).")/sequence is the one with 99% sequence identity to real one |
| Stability - Incubation |
Activity after incubation (60 minutes at 25Β°C), measured by absorbance spectrophotometry (colorimetric SomogyiβNelson assay, reducing sugars and Nelson's reagant reaction product absorbance measurement, 590 nm) in aqueous phase |
Ethanol |
15% |
100 |
94 |
% |
Incubation: ?, Assay: 100 mM sodium phosphate buffer |
Incubation: ?, Assay: 7.5 |
Incubation: 25Β°C, Assay: 50Β°C |
0.5% Soluble birchwood xylan |
Reducing sugars |
β |
Yes, "under constant agitation" incubation |
Water-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions (" The xylanase activity towards beechwood xylan was assessed utilizing aliquots of the incubated enzyme in a reaction mixture with a solvent concentration less than 1 % (v/v).")/sequence is the one with 99% sequence identity to real one |
| Stability - Incubation |
Activity after incubation (60 minutes at 25Β°C), measured by absorbance spectrophotometry (colorimetric SomogyiβNelson assay, reducing sugars and Nelson's reagant reaction product absorbance measurement, 590 nm) in aqueous phase |
Methanol |
15% |
100 |
94 |
% |
Incubation: ?, Assay: 100 mM sodium phosphate buffer |
Incubation: ?, Assay: 7.5 |
Incubation: 25Β°C, Assay: 50Β°C |
0.5% Soluble birchwood xylan |
Reducing sugars |
β |
Yes, "under constant agitation" incubation |
Water-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions (" The xylanase activity towards beechwood xylan was assessed utilizing aliquots of the incubated enzyme in a reaction mixture with a solvent concentration less than 1 % (v/v).")/sequence is the one with 99% sequence identity to real one |
| Stability - Incubation |
Activity after incubation (60 minutes at 25Β°C), measured by absorbance spectrophotometry (colorimetric SomogyiβNelson assay, reducing sugars and Nelson's reagant reaction product absorbance measurement, 590 nm) in aqueous phase |
Acetone |
15% |
100 |
97 |
% |
Incubation: ?, Assay: 100 mM sodium phosphate buffer |
Incubation: ?, Assay: 7.5 |
Incubation: 25Β°C, Assay: 50Β°C |
0.5% Soluble birchwood xylan |
Reducing sugars |
β |
Yes, "under constant agitation" incubation |
Water-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions (" The xylanase activity towards beechwood xylan was assessed utilizing aliquots of the incubated enzyme in a reaction mixture with a solvent concentration less than 1 % (v/v).")/sequence is the one with 99% sequence identity to real one |
| Stability - Incubation |
Activity after incubation (60 minutes at 25Β°C), measured by absorbance spectrophotometry (colorimetric SomogyiβNelson assay, reducing sugars and Nelson's reagant reaction product absorbance measurement, 590 nm) in aqueous phase |
Acetonitrile |
15% |
100 |
92 |
% |
Incubation: ?, Assay: 100 mM sodium phosphate buffer |
Incubation: ?, Assay: 7.5 |
Incubation: 25Β°C, Assay: 50Β°C |
0.5% Soluble birchwood xylan |
Reducing sugars |
β |
Yes, "under constant agitation" incubation |
Water-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions (" The xylanase activity towards beechwood xylan was assessed utilizing aliquots of the incubated enzyme in a reaction mixture with a solvent concentration less than 1 % (v/v).")/sequence is the one with 99% sequence identity to real one |
| Stability - Incubation |
Activity after incubation (60 minutes at 25Β°C), measured by absorbance spectrophotometry (colorimetric SomogyiβNelson assay, reducing sugars and Nelson's reagant reaction product absorbance measurement, 590 nm) in aqueous phase |
Ethyl Acetate |
15% |
100 |
98 |
% |
Incubation: ?, Assay: 100 mM sodium phosphate buffer |
Incubation: ?, Assay: 7.5 |
Incubation: 25Β°C, Assay: 50Β°C |
0.5% Soluble birchwood xylan |
Reducing sugars |
β |
Yes, "under constant agitation" incubation |
Water-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions (" The xylanase activity towards beechwood xylan was assessed utilizing aliquots of the incubated enzyme in a reaction mixture with a solvent concentration less than 1 % (v/v).")/sequence is the one with 99% sequence identity to real one |
| Stability - Incubation |
Activity after incubation (60 minutes at 25Β°C), measured by absorbance spectrophotometry (colorimetric SomogyiβNelson assay, reducing sugars and Nelson's reagant reaction product absorbance measurement, 590 nm) in aqueous phase |
Isopropanol |
15% |
100 |
95 |
% |
Incubation: ?, Assay: 100 mM sodium phosphate buffer |
Incubation: ?, Assay: 7.5 |
Incubation: 25Β°C, Assay: 50Β°C |
0.5% Soluble birchwood xylan |
Reducing sugars |
β |
Yes, "under constant agitation" incubation |
Water-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions (" The xylanase activity towards beechwood xylan was assessed utilizing aliquots of the incubated enzyme in a reaction mixture with a solvent concentration less than 1 % (v/v).")/sequence is the one with 99% sequence identity to real one |
| Stability - Incubation |
Activity after incubation (60 minutes at 25Β°C), measured by absorbance spectrophotometry (colorimetric SomogyiβNelson assay, reducing sugars and Nelson's reagant reaction product absorbance measurement, 590 nm) in aqueous phase |
Dimethyl Sulfoxide (DMSO) |
15% |
100 |
88 |
% |
Incubation: ?, Assay: 100 mM sodium phosphate buffer |
Incubation: ?, Assay: 7.5 |
Incubation: 25Β°C, Assay: 50Β°C |
0.5% Soluble birchwood xylan |
Reducing sugars |
β |
Yes, "under constant agitation" incubation |
Water-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions (" The xylanase activity towards beechwood xylan was assessed utilizing aliquots of the incubated enzyme in a reaction mixture with a solvent concentration less than 1 % (v/v).")/sequence is the one with 99% sequence identity to real one |
| Stability - Incubation |
Activity after incubation (60 minutes at 25Β°C), measured by absorbance spectrophotometry (colorimetric SomogyiβNelson assay, reducing sugars and Nelson's reagant reaction product absorbance measurement, 590 nm) in aqueous phase |
Dimethylformamide (DMF) |
15% |
100 |
99 |
% |
Incubation: ?, Assay: 100 mM sodium phosphate buffer |
Incubation: ?, Assay: 7.5 |
Incubation: 25Β°C, Assay: 50Β°C |
0.5% Soluble birchwood xylan |
Reducing sugars |
β |
Yes, "under constant agitation" incubation |
Water-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions (" The xylanase activity towards beechwood xylan was assessed utilizing aliquots of the incubated enzyme in a reaction mixture with a solvent concentration less than 1 % (v/v).")/sequence is the one with 99% sequence identity to real one |
| Stability - Incubation |
Activity after incubation (60 minutes at 25Β°C), measured by absorbance spectrophotometry (colorimetric SomogyiβNelson assay, reducing sugars and Nelson's reagant reaction product absorbance measurement, 590 nm) in aqueous phase |
1-Butanol |
15% |
100 |
100 |
% |
Incubation: ?, Assay: 100 mM sodium phosphate buffer |
Incubation: ?, Assay: 7.5 |
Incubation: 25Β°C, Assay: 50Β°C |
0.5% Soluble birchwood xylan |
Reducing sugars |
β |
Yes, "under constant agitation" incubation |
Water-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous conditions (" The xylanase activity towards beechwood xylan was assessed utilizing aliquots of the incubated enzyme in a reaction mixture with a solvent concentration less than 1 % (v/v).")/sequence is the one with 99% sequence identity to real one |