Laccase (gene LacA) from Cerrena sp. HYB07
Enzyme Description
Sequence
MAFRSAFSAFITLSLTLGALAAVGPVTDIHIVNKDIAPDGFSRPSVLAGGTFPGPLITGQKGDNFKLNVVDDLTDASMLKSTSIHWHGFFQKGTNWADGPAFVNQCPISTGNSFLYNFQVPDQAGTYWYHSHLSTQYCDGLRGAFVVYDPTDPHKALYDVDDESTVITLADWYHTLARQIVGVAIADTTLINGLGRNTNGPADAALAVINVEAGKRYRLRLVSISCDPNYVFSIDNHDFNIIEVDGVNSKPLNVDSIQIFAGQRYSAVLNANQPVGNYWVRANPNLGTTGFTGGINSAILRYKGAPVAEPTTTQTTSTKPLQEPNLRPLVSMPVPGSATPGGVDVVHNLILGFSAGKFTINGAAFTPPSVPVLLQILSGTTNAQDLLPSGSVITLPIGKTIELTLAAGVLGGPHPFHLHGHNFHVVRSAGQTTPNYVDPIVRDVVNTGGTGDNVTIRFTTDNPGPWFLHCHIDWHLEAGFAVVFAEGVNQTNAANPTPADWNNLCNIYNALADGDK
Jie Yang et al. (2014)
β Purification and Characterization of a Novel Laccase from Cerrena sp. HYB07 with Dye Decolorizing Ability
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Database ID
UniProt: A0A0A0Q5C7 β
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Escherichia coli TOP10
Experimental Data (30 measurements)
30 measurements β page 1 of 2
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Activity - Classical | Activity, measured by absorbance spectrophotometry (ABTS radical cation absorbance measurment, 420 nm) | Methanol | 5% | 100.0 | 92.9 | % | 25 mM citrate-phosphate buffe | 3 | 25Β°C | 0.5 mM ABTS | ABTS radical cation reaction products | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity, measured by absorbance spectrophotometry (ABTS radical cation absorbance measurment, 420 nm) | Dimethylformamide (DMF) | 25% | 100.0 | 78.9 | % | 25 mM citrate-phosphate buffe | 3 | 25Β°C | 0.5 mM ABTS | ABTS radical cation reaction products | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity, measured by absorbance spectrophotometry (ABTS radical cation absorbance measurment, 420 nm) | Dimethylformamide (DMF) | 10% | 100.0 | 82.5 | % | 25 mM citrate-phosphate buffe | 3 | 25Β°C | 0.5 mM ABTS | ABTS radical cation reaction products | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity, measured by absorbance spectrophotometry (ABTS radical cation absorbance measurment, 420 nm) | Dimethylformamide (DMF) | 5% | 100.0 | 95.1 | % | 25 mM citrate-phosphate buffe | 3 | 25Β°C | 0.5 mM ABTS | ABTS radical cation reaction products | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity, measured by absorbance spectrophotometry (ABTS radical cation absorbance measurment, 420 nm) | Dimethyl Sulfoxide (DMSO) | 25% | 100.0 | 58.7 | % | 25 mM citrate-phosphate buffe | 3 | 25Β°C | 0.5 mM ABTS | ABTS radical cation reaction products | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity, measured by absorbance spectrophotometry (ABTS radical cation absorbance measurment, 420 nm) | Dimethyl Sulfoxide (DMSO) | 10% | 100.0 | 77.3 | % | 25 mM citrate-phosphate buffe | 3 | 25Β°C | 0.5 mM ABTS | ABTS radical cation reaction products | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity, measured by absorbance spectrophotometry (ABTS radical cation absorbance measurment, 420 nm) | Dimethyl Sulfoxide (DMSO) | 5% | 100.0 | 91.1 | % | 25 mM citrate-phosphate buffe | 3 | 25Β°C | 0.5 mM ABTS | ABTS radical cation reaction products | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity, measured by absorbance spectrophotometry (ABTS radical cation absorbance measurment, 420 nm) | Acetonitrile | 25% | 100.0 | 66.2 | % | 25 mM citrate-phosphate buffe | 3 | 25Β°C | 0.5 mM ABTS | ABTS radical cation reaction products | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity, measured by absorbance spectrophotometry (ABTS radical cation absorbance measurment, 420 nm) | Acetonitrile | 10% | 100.0 | 96.8 | % | 25 mM citrate-phosphate buffe | 3 | 25Β°C | 0.5 mM ABTS | ABTS radical cation reaction products | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity, measured by absorbance spectrophotometry (ABTS radical cation absorbance measurment, 420 nm) | Acetonitrile | 5% | 100.0 | 102.5 | % | 25 mM citrate-phosphate buffe | 3 | 25Β°C | 0.5 mM ABTS | ABTS radical cation reaction products | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity, measured by absorbance spectrophotometry (ABTS radical cation absorbance measurment, 420 nm) | Ethanol | 25% | 100.0 | 70.9 | % | 25 mM citrate-phosphate buffe | 3 | 25Β°C | 0.5 mM ABTS | ABTS radical cation reaction products | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity, measured by absorbance spectrophotometry (ABTS radical cation absorbance measurment, 420 nm) | Ethanol | 10% | 100.0 | 83.1 | % | 25 mM citrate-phosphate buffe | 3 | 25Β°C | 0.5 mM ABTS | ABTS radical cation reaction products | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity, measured by absorbance spectrophotometry (ABTS radical cation absorbance measurment, 420 nm) | Ethanol | 5% | 100.0 | 101.0 | % | 25 mM citrate-phosphate buffe | 3 | 25Β°C | 0.5 mM ABTS | ABTS radical cation reaction products | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity, measured by absorbance spectrophotometry (ABTS radical cation absorbance measurment, 420 nm) | Methanol | 25% | 100.0 | 57.3 | % | 25 mM citrate-phosphate buffe | 3 | 25Β°C | 0.5 mM ABTS | ABTS radical cation reaction products | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity, measured by absorbance spectrophotometry (ABTS radical cation absorbance measurment, 420 nm) | Methanol | 10% | 100.0 | 72.7 | % | 25 mM citrate-phosphate buffe | 3 | 25Β°C | 0.5 mM ABTS | ABTS radical cation reaction products | β | β | Classical aqueous control (in %) |
| Stability - Incubation | Activity after incubation (4 hours at 25Β°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurment, 420 nm) in aqueous phase | Methanol | 5% | 100.0 | 92.7 | % | Incubation: ?, Assay: 25 mM citrate-phosphate buffer | Incubation: ?, Assay: 3 | Incubation: 25Β°C, Assay: 25Β°C | 0.5 mM ABTS | ABTS radical cation reaction products | β | β | Non-incubated control (in %), assay measured in aqueous phase | Uncertainty about control |
| Stability - Incubation | Activity after incubation (4 hours at 25Β°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurment, 420 nm) in aqueous phase | Dimethylformamide (DMF) | 25% | 100.0 | 94.4 | % | Incubation: ?, Assay: 25 mM citrate-phosphate buffer | Incubation: ?, Assay: 3 | Incubation: 25Β°C, Assay: 25Β°C | 0.5 mM ABTS | ABTS radical cation reaction products | β | β | Non-incubated control (in %), assay measured in aqueous phase | Uncertainty about control |
| Stability - Incubation | Activity after incubation (4 hours at 25Β°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurment, 420 nm) in aqueous phase | Dimethylformamide (DMF) | 10% | 100.0 | 100.7 | % | Incubation: ?, Assay: 25 mM citrate-phosphate buffer | Incubation: ?, Assay: 3 | Incubation: 25Β°C, Assay: 25Β°C | 0.5 mM ABTS | ABTS radical cation reaction products | β | β | Non-incubated control (in %), assay measured in aqueous phase | Uncertainty about control |
| Stability - Incubation | Activity after incubation (4 hours at 25Β°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurment, 420 nm) in aqueous phase | Dimethylformamide (DMF) | 5% | 100.0 | 95.9 | % | Incubation: ?, Assay: 25 mM citrate-phosphate buffer | Incubation: ?, Assay: 3 | Incubation: 25Β°C, Assay: 25Β°C | 0.5 mM ABTS | ABTS radical cation reaction products | β | β | Non-incubated control (in %), assay measured in aqueous phase | Uncertainty about control |
| Stability - Incubation | Activity after incubation (4 hours at 25Β°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurment, 420 nm) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 25% | 100.0 | 124.9 | % | Incubation: ?, Assay: 25 mM citrate-phosphate buffer | Incubation: ?, Assay: 3 | Incubation: 25Β°C, Assay: 25Β°C | 0.5 mM ABTS | ABTS radical cation reaction products | β | β | Non-incubated control (in %), assay measured in aqueous phase | Uncertainty about control |
Visualization : Activity β Classical
One bar per measurement. Colour = solvent, shade = solvent volume.
Visualization : Stability β Incubation
One bar per measurement. Colour = solvent, shade = solvent volume.