Laccase (gene LacA) from Cerrena sp. HYB07

Enzyme Description

Species

Extremophile

EC Number

1.10.3.2 ↗

Sequence

Length: 516 amino acids

MAFRSAFSAFITLSLTLGALAAVGPVTDIHIVNKDIAPDGFSRPSVLAGGTFPGPLITGQKGDNFKLNVVDDLTDASMLKSTSIHWHGFFQKGTNWADGPAFVNQCPISTGNSFLYNFQVPDQAGTYWYHSHLSTQYCDGLRGAFVVYDPTDPHKALYDVDDESTVITLADWYHTLARQIVGVAIADTTLINGLGRNTNGPADAALAVINVEAGKRYRLRLVSISCDPNYVFSIDNHDFNIIEVDGVNSKPLNVDSIQIFAGQRYSAVLNANQPVGNYWVRANPNLGTTGFTGGINSAILRYKGAPVAEPTTTQTTSTKPLQEPNLRPLVSMPVPGSATPGGVDVVHNLILGFSAGKFTINGAAFTPPSVPVLLQILSGTTNAQDLLPSGSVITLPIGKTIELTLAAGVLGGPHPFHLHGHNFHVVRSAGQTTPNYVDPIVRDVVNTGGTGDNVTIRFTTDNPGPWFLHCHIDWHLEAGFAVVFAEGVNQTNAANPTPADWNNLCNIYNALADGDK

Jie Yang et al. (2014) — Purification and Characterization of a Novel Laccase from Cerrena sp. HYB07 with Dye Decolorizing Ability

PLoS ONE · doi:10.1371/journal.pone.0110834 ↗ · Activity - Classical Stability - Incubation

30 measurements ▼

Database ID

UniProt: A0A0A0Q5C7 ↗

Sequence Annotation

Explicit - Provided

Protein Source

Recombinant, host bacterium Escherichia coli TOP10

Experimental Data (30 measurements)

30 measurements — page 2 of 2

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Stability - Incubation	Activity after incubation (4 hours at 25°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurment, 420 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	10%	100.0	116.8	%	Incubation: ?, Assay: 25 mM citrate-phosphate buffer	Incubation: ?, Assay: 3	Incubation: 25°C, Assay: 25°C	0.5 mM ABTS	ABTS radical cation reaction products	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Uncertainty about control
Stability - Incubation	Activity after incubation (4 hours at 25°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurment, 420 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	5%	100.0	98.1	%	Incubation: ?, Assay: 25 mM citrate-phosphate buffer	Incubation: ?, Assay: 3	Incubation: 25°C, Assay: 25°C	0.5 mM ABTS	ABTS radical cation reaction products	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Uncertainty about control
Stability - Incubation	Activity after incubation (4 hours at 25°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurment, 420 nm) in aqueous phase	Acetonitrile	25%	100.0	50.4	%	Incubation: ?, Assay: 25 mM citrate-phosphate buffer	Incubation: ?, Assay: 3	Incubation: 25°C, Assay: 25°C	0.5 mM ABTS	ABTS radical cation reaction products	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Uncertainty about control
Stability - Incubation	Activity after incubation (4 hours at 25°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurment, 420 nm) in aqueous phase	Acetonitrile	10%	100.0	95.9	%	Incubation: ?, Assay: 25 mM citrate-phosphate buffer	Incubation: ?, Assay: 3	Incubation: 25°C, Assay: 25°C	0.5 mM ABTS	ABTS radical cation reaction products	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Uncertainty about control
Stability - Incubation	Activity after incubation (4 hours at 25°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurment, 420 nm) in aqueous phase	Acetonitrile	5%	100.0	105.0	%	Incubation: ?, Assay: 25 mM citrate-phosphate buffer	Incubation: ?, Assay: 3	Incubation: 25°C, Assay: 25°C	0.5 mM ABTS	ABTS radical cation reaction products	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Uncertainty about control
Stability - Incubation	Activity after incubation (4 hours at 25°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurment, 420 nm) in aqueous phase	Ethanol	25%	100.0	100.2	%	Incubation: ?, Assay: 25 mM citrate-phosphate buffer	Incubation: ?, Assay: 3	Incubation: 25°C, Assay: 25°C	0.5 mM ABTS	ABTS radical cation reaction products	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Uncertainty about control
Stability - Incubation	Activity after incubation (4 hours at 25°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurment, 420 nm) in aqueous phase	Ethanol	10%	100.0	101.0	%	Incubation: ?, Assay: 25 mM citrate-phosphate buffer	Incubation: ?, Assay: 3	Incubation: 25°C, Assay: 25°C	0.5 mM ABTS	ABTS radical cation reaction products	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Uncertainty about control
Stability - Incubation	Activity after incubation (4 hours at 25°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurment, 420 nm) in aqueous phase	Ethanol	5%	100.0	94.0	%	Incubation: ?, Assay: 25 mM citrate-phosphate buffer	Incubation: ?, Assay: 3	Incubation: 25°C, Assay: 25°C	0.5 mM ABTS	ABTS radical cation reaction products	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Uncertainty about control
Stability - Incubation	Activity after incubation (4 hours at 25°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurment, 420 nm) in aqueous phase	Methanol	25%	100.0	116.5	%	Incubation: ?, Assay: 25 mM citrate-phosphate buffer	Incubation: ?, Assay: 3	Incubation: 25°C, Assay: 25°C	0.5 mM ABTS	ABTS radical cation reaction products	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Uncertainty about control
Stability - Incubation	Activity after incubation (4 hours at 25°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurment, 420 nm) in aqueous phase	Methanol	10%	100.0	107.7	%	Incubation: ?, Assay: 25 mM citrate-phosphate buffer	Incubation: ?, Assay: 3	Incubation: 25°C, Assay: 25°C	0.5 mM ABTS	ABTS radical cation reaction products	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Uncertainty about control

Activity - Classical	Activity, measured by absorbance spectrophotometry (ABTS radical cation absorbance measurment, 420 nm)	Methanol	5%	100.0	92.9	%	25 mM citrate-phosphate buffe	3	25°C	0.5 mM ABTS	ABTS radical cation reaction products	—	—	Classical aqueous control (in %)
Activity - Classical	Activity, measured by absorbance spectrophotometry (ABTS radical cation absorbance measurment, 420 nm)	Dimethylformamide (DMF)	25%	100.0	78.9	%	25 mM citrate-phosphate buffe	3	25°C	0.5 mM ABTS	ABTS radical cation reaction products	—	—	Classical aqueous control (in %)
Activity - Classical	Activity, measured by absorbance spectrophotometry (ABTS radical cation absorbance measurment, 420 nm)	Dimethylformamide (DMF)	10%	100.0	82.5	%	25 mM citrate-phosphate buffe	3	25°C	0.5 mM ABTS	ABTS radical cation reaction products	—	—	Classical aqueous control (in %)
Activity - Classical	Activity, measured by absorbance spectrophotometry (ABTS radical cation absorbance measurment, 420 nm)	Dimethylformamide (DMF)	5%	100.0	95.1	%	25 mM citrate-phosphate buffe	3	25°C	0.5 mM ABTS	ABTS radical cation reaction products	—	—	Classical aqueous control (in %)
Activity - Classical	Activity, measured by absorbance spectrophotometry (ABTS radical cation absorbance measurment, 420 nm)	Dimethyl Sulfoxide (DMSO)	25%	100.0	58.7	%	25 mM citrate-phosphate buffe	3	25°C	0.5 mM ABTS	ABTS radical cation reaction products	—	—	Classical aqueous control (in %)
Activity - Classical	Activity, measured by absorbance spectrophotometry (ABTS radical cation absorbance measurment, 420 nm)	Dimethyl Sulfoxide (DMSO)	10%	100.0	77.3	%	25 mM citrate-phosphate buffe	3	25°C	0.5 mM ABTS	ABTS radical cation reaction products	—	—	Classical aqueous control (in %)
Activity - Classical	Activity, measured by absorbance spectrophotometry (ABTS radical cation absorbance measurment, 420 nm)	Dimethyl Sulfoxide (DMSO)	5%	100.0	91.1	%	25 mM citrate-phosphate buffe	3	25°C	0.5 mM ABTS	ABTS radical cation reaction products	—	—	Classical aqueous control (in %)
Activity - Classical	Activity, measured by absorbance spectrophotometry (ABTS radical cation absorbance measurment, 420 nm)	Acetonitrile	25%	100.0	66.2	%	25 mM citrate-phosphate buffe	3	25°C	0.5 mM ABTS	ABTS radical cation reaction products	—	—	Classical aqueous control (in %)
Activity - Classical	Activity, measured by absorbance spectrophotometry (ABTS radical cation absorbance measurment, 420 nm)	Acetonitrile	10%	100.0	96.8	%	25 mM citrate-phosphate buffe	3	25°C	0.5 mM ABTS	ABTS radical cation reaction products	—	—	Classical aqueous control (in %)
Activity - Classical	Activity, measured by absorbance spectrophotometry (ABTS radical cation absorbance measurment, 420 nm)	Acetonitrile	5%	100.0	102.5	%	25 mM citrate-phosphate buffe	3	25°C	0.5 mM ABTS	ABTS radical cation reaction products	—	—	Classical aqueous control (in %)
Activity - Classical	Activity, measured by absorbance spectrophotometry (ABTS radical cation absorbance measurment, 420 nm)	Ethanol	25%	100.0	70.9	%	25 mM citrate-phosphate buffe	3	25°C	0.5 mM ABTS	ABTS radical cation reaction products	—	—	Classical aqueous control (in %)
Activity - Classical	Activity, measured by absorbance spectrophotometry (ABTS radical cation absorbance measurment, 420 nm)	Ethanol	10%	100.0	83.1	%	25 mM citrate-phosphate buffe	3	25°C	0.5 mM ABTS	ABTS radical cation reaction products	—	—	Classical aqueous control (in %)
Activity - Classical	Activity, measured by absorbance spectrophotometry (ABTS radical cation absorbance measurment, 420 nm)	Ethanol	5%	100.0	101.0	%	25 mM citrate-phosphate buffe	3	25°C	0.5 mM ABTS	ABTS radical cation reaction products	—	—	Classical aqueous control (in %)
Activity - Classical	Activity, measured by absorbance spectrophotometry (ABTS radical cation absorbance measurment, 420 nm)	Methanol	25%	100.0	57.3	%	25 mM citrate-phosphate buffe	3	25°C	0.5 mM ABTS	ABTS radical cation reaction products	—	—	Classical aqueous control (in %)
Activity - Classical	Activity, measured by absorbance spectrophotometry (ABTS radical cation absorbance measurment, 420 nm)	Methanol	10%	100.0	72.7	%	25 mM citrate-phosphate buffe	3	25°C	0.5 mM ABTS	ABTS radical cation reaction products	—	—	Classical aqueous control (in %)
Stability - Incubation	Activity after incubation (4 hours at 25°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurment, 420 nm) in aqueous phase	Methanol	5%	100.0	92.7	%	Incubation: ?, Assay: 25 mM citrate-phosphate buffer	Incubation: ?, Assay: 3	Incubation: 25°C, Assay: 25°C	0.5 mM ABTS	ABTS radical cation reaction products	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Uncertainty about control
Stability - Incubation	Activity after incubation (4 hours at 25°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurment, 420 nm) in aqueous phase	Dimethylformamide (DMF)	25%	100.0	94.4	%	Incubation: ?, Assay: 25 mM citrate-phosphate buffer	Incubation: ?, Assay: 3	Incubation: 25°C, Assay: 25°C	0.5 mM ABTS	ABTS radical cation reaction products	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Uncertainty about control
Stability - Incubation	Activity after incubation (4 hours at 25°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurment, 420 nm) in aqueous phase	Dimethylformamide (DMF)	10%	100.0	100.7	%	Incubation: ?, Assay: 25 mM citrate-phosphate buffer	Incubation: ?, Assay: 3	Incubation: 25°C, Assay: 25°C	0.5 mM ABTS	ABTS radical cation reaction products	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Uncertainty about control
Stability - Incubation	Activity after incubation (4 hours at 25°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurment, 420 nm) in aqueous phase	Dimethylformamide (DMF)	5%	100.0	95.9	%	Incubation: ?, Assay: 25 mM citrate-phosphate buffer	Incubation: ?, Assay: 3	Incubation: 25°C, Assay: 25°C	0.5 mM ABTS	ABTS radical cation reaction products	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Uncertainty about control
Stability - Incubation	Activity after incubation (4 hours at 25°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurment, 420 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	25%	100.0	124.9	%	Incubation: ?, Assay: 25 mM citrate-phosphate buffer	Incubation: ?, Assay: 3	Incubation: 25°C, Assay: 25°C	0.5 mM ABTS	ABTS radical cation reaction products	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Uncertainty about control
Stability - Incubation	Activity after incubation (4 hours at 25°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurment, 420 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	10%	100.0	116.8	%	Incubation: ?, Assay: 25 mM citrate-phosphate buffer	Incubation: ?, Assay: 3	Incubation: 25°C, Assay: 25°C	0.5 mM ABTS	ABTS radical cation reaction products	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Uncertainty about control
Stability - Incubation	Activity after incubation (4 hours at 25°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurment, 420 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	5%	100.0	98.1	%	Incubation: ?, Assay: 25 mM citrate-phosphate buffer	Incubation: ?, Assay: 3	Incubation: 25°C, Assay: 25°C	0.5 mM ABTS	ABTS radical cation reaction products	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Uncertainty about control
Stability - Incubation	Activity after incubation (4 hours at 25°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurment, 420 nm) in aqueous phase	Acetonitrile	25%	100.0	50.4	%	Incubation: ?, Assay: 25 mM citrate-phosphate buffer	Incubation: ?, Assay: 3	Incubation: 25°C, Assay: 25°C	0.5 mM ABTS	ABTS radical cation reaction products	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Uncertainty about control
Stability - Incubation	Activity after incubation (4 hours at 25°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurment, 420 nm) in aqueous phase	Acetonitrile	10%	100.0	95.9	%	Incubation: ?, Assay: 25 mM citrate-phosphate buffer	Incubation: ?, Assay: 3	Incubation: 25°C, Assay: 25°C	0.5 mM ABTS	ABTS radical cation reaction products	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Uncertainty about control
Stability - Incubation	Activity after incubation (4 hours at 25°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurment, 420 nm) in aqueous phase	Acetonitrile	5%	100.0	105.0	%	Incubation: ?, Assay: 25 mM citrate-phosphate buffer	Incubation: ?, Assay: 3	Incubation: 25°C, Assay: 25°C	0.5 mM ABTS	ABTS radical cation reaction products	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Uncertainty about control
Stability - Incubation	Activity after incubation (4 hours at 25°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurment, 420 nm) in aqueous phase	Ethanol	25%	100.0	100.2	%	Incubation: ?, Assay: 25 mM citrate-phosphate buffer	Incubation: ?, Assay: 3	Incubation: 25°C, Assay: 25°C	0.5 mM ABTS	ABTS radical cation reaction products	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Uncertainty about control
Stability - Incubation	Activity after incubation (4 hours at 25°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurment, 420 nm) in aqueous phase	Ethanol	10%	100.0	101.0	%	Incubation: ?, Assay: 25 mM citrate-phosphate buffer	Incubation: ?, Assay: 3	Incubation: 25°C, Assay: 25°C	0.5 mM ABTS	ABTS radical cation reaction products	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Uncertainty about control
Stability - Incubation	Activity after incubation (4 hours at 25°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurment, 420 nm) in aqueous phase	Ethanol	5%	100.0	94.0	%	Incubation: ?, Assay: 25 mM citrate-phosphate buffer	Incubation: ?, Assay: 3	Incubation: 25°C, Assay: 25°C	0.5 mM ABTS	ABTS radical cation reaction products	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Uncertainty about control
Stability - Incubation	Activity after incubation (4 hours at 25°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurment, 420 nm) in aqueous phase	Methanol	25%	100.0	116.5	%	Incubation: ?, Assay: 25 mM citrate-phosphate buffer	Incubation: ?, Assay: 3	Incubation: 25°C, Assay: 25°C	0.5 mM ABTS	ABTS radical cation reaction products	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Uncertainty about control
Stability - Incubation	Activity after incubation (4 hours at 25°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurment, 420 nm) in aqueous phase	Methanol	10%	100.0	107.7	%	Incubation: ?, Assay: 25 mM citrate-phosphate buffer	Incubation: ?, Assay: 3	Incubation: 25°C, Assay: 25°C	0.5 mM ABTS	ABTS radical cation reaction products	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Uncertainty about control

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Visualization : Activity — Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Visualization : Stability — Incubation