Lipase from Bacillus sp. DR90

Enzyme Description

Species
Bacillus sp. DR90 β†—
Extremophile
No but "thermophilic" and "organic-solvent tolerant" enzyme
EC Number
3.1.1.3 β†—

Sequence

Length: 212 amino acids
MKFVKRRIIALVTILMLSVTSLFALQPSAKAAEHNPVVMVHGIGGASFNFAGIKSYLVSQGWSRDKLYAVDFWDKTGTNYNNGPVLSRFVQKVLDETGAKKVDIVAHSMGGANTLYYIKNLDGGNKVANVVTLGGANRLTTGKALPGTDPNQKILYTSIYSSADMIVMNYLSRLDGARNVQIHGVGHIGLLYSSQVNSLIKEGLNGGGQNTN
Ahmad Asoodeh et al. (2014) β€” Expression and Biochemical Characterization of a Thermophilic Organic Solvent-Tolerant Lipase from Bacillus sp. DR90
The Protein Journal  Β· doi:10.1007/s10930-014-9574-x β†—  Β· Stability - Incubation
22 measurements ▼
Database ID
UniProt: A0A067Y841 β†—
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (22 measurements)

22 measurements β€” page 1 of 2
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (30 minutes at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase Diethyl Ether 20% 100 128 % Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol Incubation: ?, Assay: 7.5 Incubation: 25Β°C, Assay: 50Β°C 0.1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 150 rpm Water-incubated control (in %), assay in aqueous phase | Clear about assay
Stability - Incubation Activity after incubation (30 minutes at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase n-Heptane 20% 100 120 % Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol Incubation: ?, Assay: 7.5 Incubation: 25Β°C, Assay: 50Β°C 0.1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 150 rpm Water-incubated control (in %), assay in aqueous phase | Clear about assay
Stability - Incubation Activity after incubation (30 minutes at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase n-Heptane 10% 100 98 % Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol Incubation: ?, Assay: 7.5 Incubation: 25Β°C, Assay: 50Β°C 0.1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 150 rpm Water-incubated control (in %), assay in aqueous phase | Clear about assay
Stability - Incubation Activity after incubation (30 minutes at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase n-Hexane 20% 100 110 % Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol Incubation: ?, Assay: 7.5 Incubation: 25Β°C, Assay: 50Β°C 0.1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 150 rpm Water-incubated control (in %), assay in aqueous phase | Clear about assay
Stability - Incubation Activity after incubation (30 minutes at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase n-Hexane 10% 100 97 % Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol Incubation: ?, Assay: 7.5 Incubation: 25Β°C, Assay: 50Β°C 0.1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 150 rpm Water-incubated control (in %), assay in aqueous phase | Clear about assay
Stability - Incubation Activity after incubation (30 minutes at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase Toluene 20% 100 118 % Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol Incubation: ?, Assay: 7.5 Incubation: 25Β°C, Assay: 50Β°C 0.1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 150 rpm Water-incubated control (in %), assay in aqueous phase | Clear about assay
Stability - Incubation Activity after incubation (30 minutes at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase Toluene 10% 100 101 % Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol Incubation: ?, Assay: 7.5 Incubation: 25Β°C, Assay: 50Β°C 0.1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 150 rpm Water-incubated control (in %), assay in aqueous phase | Clear about assay
Stability - Incubation Activity after incubation (30 minutes at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase Chloroform 20% 100 95 % Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol Incubation: ?, Assay: 7.5 Incubation: 25Β°C, Assay: 50Β°C 0.1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 150 rpm Water-incubated control (in %), assay in aqueous phase | Clear about assay
Stability - Incubation Activity after incubation (30 minutes at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase Chloroform 10% 100 93 % Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol Incubation: ?, Assay: 7.5 Incubation: 25Β°C, Assay: 50Β°C 0.1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 150 rpm Water-incubated control (in %), assay in aqueous phase | Clear about assay
Stability - Incubation Activity after incubation (30 minutes at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase Isoamyl Alcohol 20% 100 81 % Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol Incubation: ?, Assay: 7.5 Incubation: 25Β°C, Assay: 50Β°C 0.1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 150 rpm Water-incubated control (in %), assay in aqueous phase | Clear about assay
Stability - Incubation Activity after incubation (30 minutes at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase Isoamyl Alcohol 10% 100 85 % Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol Incubation: ?, Assay: 7.5 Incubation: 25Β°C, Assay: 50Β°C 0.1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 150 rpm Water-incubated control (in %), assay in aqueous phase | Clear about assay
Stability - Incubation Activity after incubation (30 minutes at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase Methanol 10% 100 96 % Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol Incubation: ?, Assay: 7.5 Incubation: 25Β°C, Assay: 50Β°C 0.1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 150 rpm Water-incubated control (in %), assay in aqueous phase | Clear about assay
Stability - Incubation Activity after incubation (30 minutes at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase Diethyl Ether 10% 100 112 % Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol Incubation: ?, Assay: 7.5 Incubation: 25Β°C, Assay: 50Β°C 0.1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 150 rpm Water-incubated control (in %), assay in aqueous phase | Clear about assay
Stability - Incubation Activity after incubation (30 minutes at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase 1-Butanol 20% 100 90 % Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol Incubation: ?, Assay: 7.5 Incubation: 25Β°C, Assay: 50Β°C 0.1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 150 rpm Water-incubated control (in %), assay in aqueous phase | Clear about assay
Stability - Incubation Activity after incubation (30 minutes at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase 1-Butanol 10% 100 91 % Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol Incubation: ?, Assay: 7.5 Incubation: 25Β°C, Assay: 50Β°C 0.1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 150 rpm Water-incubated control (in %), assay in aqueous phase | Clear about assay
Stability - Incubation Activity after incubation (30 minutes at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase Isopropanol 20% 100 98 % Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol Incubation: ?, Assay: 7.5 Incubation: 25Β°C, Assay: 50Β°C 0.1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 150 rpm Water-incubated control (in %), assay in aqueous phase | Clear about assay
Stability - Incubation Activity after incubation (30 minutes at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase Isopropanol 10% 100 81 % Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol Incubation: ?, Assay: 7.5 Incubation: 25Β°C, Assay: 50Β°C 0.1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 150 rpm Water-incubated control (in %), assay in aqueous phase | Clear about assay
Stability - Incubation Activity after incubation (30 minutes at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase Acetone 20% 100 90 % Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol Incubation: ?, Assay: 7.5 Incubation: 25Β°C, Assay: 50Β°C 0.1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 150 rpm Water-incubated control (in %), assay in aqueous phase | Clear about assay
Stability - Incubation Activity after incubation (30 minutes at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase Acetone 10% 100 76 % Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol Incubation: ?, Assay: 7.5 Incubation: 25Β°C, Assay: 50Β°C 0.1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 150 rpm Water-incubated control (in %), assay in aqueous phase | Clear about assay
Stability - Incubation Activity after incubation (30 minutes at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase Ethanol 20% 100 81 % Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol Incubation: ?, Assay: 7.5 Incubation: 25Β°C, Assay: 50Β°C 0.1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 150 rpm Water-incubated control (in %), assay in aqueous phase | Clear about assay
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Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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