Lipase from Bacillus sp. DR90

Enzyme Description

Species

Bacillus sp. DR90 ↗

Extremophile

No but "thermophilic" and "organic-solvent tolerant" enzyme

EC Number

3.1.1.3 ↗

Sequence

Length: 212 amino acids

MKFVKRRIIALVTILMLSVTSLFALQPSAKAAEHNPVVMVHGIGGASFNFAGIKSYLVSQGWSRDKLYAVDFWDKTGTNYNNGPVLSRFVQKVLDETGAKKVDIVAHSMGGANTLYYIKNLDGGNKVANVVTLGGANRLTTGKALPGTDPNQKILYTSIYSSADMIVMNYLSRLDGARNVQIHGVGHIGLLYSSQVNSLIKEGLNGGGQNTN

Ahmad Asoodeh et al. (2014) — Expression and Biochemical Characterization of a Thermophilic Organic Solvent-Tolerant Lipase from Bacillus sp. DR90

The Protein Journal · doi:10.1007/s10930-014-9574-x ↗ · Stability - Incubation

22 measurements ▼

Database ID

UniProt: A0A067Y841 ↗

Sequence Annotation

Explicit - Provided GenBank Accession Number

Protein Source

Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (22 measurements)

22 measurements — page 2 of 2

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase	Ethanol	10%	100	85	%	Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol	Incubation: ?, Assay: 7.5	Incubation: 25°C, Assay: 50°C	0.1 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	Incubation: 150 rpm	Water-incubated control (in %), assay in aqueous phase \| Clear about assay
Stability - Incubation	Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase	Methanol	20%	100	94	%	Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol	Incubation: ?, Assay: 7.5	Incubation: 25°C, Assay: 50°C	0.1 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	Incubation: 150 rpm	Water-incubated control (in %), assay in aqueous phase \| Clear about assay

Stability - Incubation

Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase

Diethyl Ether

20%

100

128

Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol

Incubation: ?, Assay: 7.5

Incubation: 25°C, Assay: 50°C

0.1 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 150 rpm

Water-incubated control (in %), assay in aqueous phase | Clear about assay

Stability - Incubation

Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase

n-Heptane

20%

100

120

Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol

Incubation: ?, Assay: 7.5

Incubation: 25°C, Assay: 50°C

0.1 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 150 rpm

Water-incubated control (in %), assay in aqueous phase | Clear about assay

Stability - Incubation

Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase

n-Heptane

10%

100

Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol

Incubation: ?, Assay: 7.5

Incubation: 25°C, Assay: 50°C

0.1 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 150 rpm

Water-incubated control (in %), assay in aqueous phase | Clear about assay

Stability - Incubation

Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase

n-Hexane

20%

100

110

Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol

Incubation: ?, Assay: 7.5

Incubation: 25°C, Assay: 50°C

0.1 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 150 rpm

Water-incubated control (in %), assay in aqueous phase | Clear about assay

Stability - Incubation

Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase

n-Hexane

10%

100

Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol

Incubation: ?, Assay: 7.5

Incubation: 25°C, Assay: 50°C

0.1 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 150 rpm

Water-incubated control (in %), assay in aqueous phase | Clear about assay

Stability - Incubation

Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase

Toluene

20%

100

118

Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol

Incubation: ?, Assay: 7.5

Incubation: 25°C, Assay: 50°C

0.1 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 150 rpm

Water-incubated control (in %), assay in aqueous phase | Clear about assay

Stability - Incubation

Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase

Toluene

10%

100

101

Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol

Incubation: ?, Assay: 7.5

Incubation: 25°C, Assay: 50°C

0.1 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 150 rpm

Water-incubated control (in %), assay in aqueous phase | Clear about assay

Stability - Incubation

Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase

Chloroform

20%

100

Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol

Incubation: ?, Assay: 7.5

Incubation: 25°C, Assay: 50°C

0.1 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 150 rpm

Water-incubated control (in %), assay in aqueous phase | Clear about assay

Stability - Incubation

Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase

Chloroform

10%

100

Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol

Incubation: ?, Assay: 7.5

Incubation: 25°C, Assay: 50°C

0.1 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 150 rpm

Water-incubated control (in %), assay in aqueous phase | Clear about assay

Stability - Incubation

Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase

Isoamyl Alcohol

20%

100

Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol

Incubation: ?, Assay: 7.5

Incubation: 25°C, Assay: 50°C

0.1 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 150 rpm

Water-incubated control (in %), assay in aqueous phase | Clear about assay

Stability - Incubation

Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase

Isoamyl Alcohol

10%

100

Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol

Incubation: ?, Assay: 7.5

Incubation: 25°C, Assay: 50°C

0.1 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 150 rpm

Water-incubated control (in %), assay in aqueous phase | Clear about assay

Stability - Incubation

Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase

Methanol

10%

100

Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol

Incubation: ?, Assay: 7.5

Incubation: 25°C, Assay: 50°C

0.1 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 150 rpm

Water-incubated control (in %), assay in aqueous phase | Clear about assay

Stability - Incubation

Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase

Diethyl Ether

10%

100

112

Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol

Incubation: ?, Assay: 7.5

Incubation: 25°C, Assay: 50°C

0.1 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 150 rpm

Water-incubated control (in %), assay in aqueous phase | Clear about assay

Stability - Incubation

Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase

1-Butanol

20%

100

Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol

Incubation: ?, Assay: 7.5

Incubation: 25°C, Assay: 50°C

0.1 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 150 rpm

Water-incubated control (in %), assay in aqueous phase | Clear about assay

Stability - Incubation

Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase

1-Butanol

10%

100

Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol

Incubation: ?, Assay: 7.5

Incubation: 25°C, Assay: 50°C

0.1 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 150 rpm

Water-incubated control (in %), assay in aqueous phase | Clear about assay

Stability - Incubation

Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase

Isopropanol

20%

100

Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol

Incubation: ?, Assay: 7.5

Incubation: 25°C, Assay: 50°C

0.1 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 150 rpm

Water-incubated control (in %), assay in aqueous phase | Clear about assay

Stability - Incubation

Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase

Isopropanol

10%

100

Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol

Incubation: ?, Assay: 7.5

Incubation: 25°C, Assay: 50°C

0.1 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 150 rpm

Water-incubated control (in %), assay in aqueous phase | Clear about assay

Stability - Incubation

Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase

Acetone

20%

100

Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol

Incubation: ?, Assay: 7.5

Incubation: 25°C, Assay: 50°C

0.1 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 150 rpm

Water-incubated control (in %), assay in aqueous phase | Clear about assay

Stability - Incubation

Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase

Acetone

10%

100

Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol

Incubation: ?, Assay: 7.5

Incubation: 25°C, Assay: 50°C

0.1 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 150 rpm

Water-incubated control (in %), assay in aqueous phase | Clear about assay

Stability - Incubation

Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase

Ethanol

20%

100

Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol

Incubation: ?, Assay: 7.5

Incubation: 25°C, Assay: 50°C

0.1 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 150 rpm

Water-incubated control (in %), assay in aqueous phase | Clear about assay

Stability - Incubation

Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase

Ethanol

10%

100

Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol

Incubation: ?, Assay: 7.5

Incubation: 25°C, Assay: 50°C

0.1 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 150 rpm

Water-incubated control (in %), assay in aqueous phase | Clear about assay

Stability - Incubation

Activity after incubation (30 minutes at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurment, 405 nm) in aqueous phase

Methanol

20%

100

Incubation: ?, Assay: 50 mM potassium phosphate buffer, 1% acetonitrile, 4% Ethanol

Incubation: ?, Assay: 7.5

Incubation: 25°C, Assay: 50°C

0.1 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 150 rpm

Water-incubated control (in %), assay in aqueous phase | Clear about assay

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Visualization : Stability — Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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Lipase from Bacillus sp. DR90

Enzyme Description

Sequence

Experimental Data (22 measurements)

Visualization : Stability — Incubation

Structure

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