Esterase (gene EstB) from Alcanivorax dieselolei B-5(T)
Enzyme Description
Extremophile
No
but"cold-adapted" enzyme
EC Number
Sequence
MAFFPVRRHWLALLGTTVVLTGCAHFSDSDAPPASMEIARGPAGLDFYTPPSPLPEGHHGDLIRARVLENNAALPSAAQNWLTLYRSTDLDGNAIAVSGTVVIPKGTPPEGGWPVISWTHGTTGIADRCAPSRNDDDYPARGYVDLMNETLDQWVRRGYAVVKTDYQGLGTPGVHPYLVGEAEARSAIDMVRAARQLTPDLSRDWLVMGHSQGGQAALYTAYLGPIYAPELNLTGAIAISPASHLSAQIQYALTHPEIRSNPYGALMLVGITAATPKVDLSTLLTEEGRKRIKQVDERCVGEMRGEDGWTLTVAELSDLEADWRPLVQAVASLEDTENLRPSVPLLILQSDDDKAVLKPLTDKLVARYQSLGLDVEYRTYHIENQDQAYTSHQITVPVSRPAAMVWAQRHLPAGR
Shanshan Zhang et al. (2013)
β Characterization of EstB, a novel cold-active and organic solvent-tolerant esterase from marine microorganism Alcanivorax dieselolei B-5(T)
Extremophiles
Β· doi:10.1007/s00792-013-0612-y β
Β· Stability - Incubation
▼
Database ID
UniProt: K0C9J9 β
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)
Experimental Data (32 measurements)
32 measurements β page 1 of 2
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Stability - Incubation | Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase | Ethylene Glycol | 50% | 100.0 | 133.9 | % | Incubation: ?, Assay: 50 mM Tris-HCl, 1% Ethanol | Incubation: ?, Assay: 8.5 | Incubation: Room temperature, Assay: 20Β°C | 0.2 mM p-Nitrophenyl hexanoate | Hexanoic Acid , p-Nitrophenol | β | β | Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase |
| Stability - Incubation | Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase | Acetonitrile | 70% | 100.0 | 6.2 | % | Incubation: ?, Assay: 50 mM Tris-HCl, 1% Ethanol | Incubation: ?, Assay: 8.5 | Incubation: Room temperature, Assay: 20Β°C | 0.2 mM p-Nitrophenyl hexanoate | Hexanoic Acid , p-Nitrophenol | β | β | Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase |
| Stability - Incubation | Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase | Methanol | 70% | 100.0 | 21.5 | % | Incubation: ?, Assay: 50 mM Tris-HCl, 1% Ethanol | Incubation: ?, Assay: 8.5 | Incubation: Room temperature, Assay: 20Β°C | 0.2 mM p-Nitrophenyl hexanoate | Hexanoic Acid , p-Nitrophenol | β | β | Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase |
| Stability - Incubation | Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 70% | 100.0 | 37.7 | % | Incubation: ?, Assay: 50 mM Tris-HCl, 1% Ethanol | Incubation: ?, Assay: 8.5 | Incubation: Room temperature, Assay: 20Β°C | 0.2 mM p-Nitrophenyl hexanoate | Hexanoic Acid , p-Nitrophenol | β | β | Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase |
| Stability - Incubation | Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase | Ethanol | 70% | 100.0 | 45.1 | % | Incubation: ?, Assay: 50 mM Tris-HCl, 1% Ethanol | Incubation: ?, Assay: 8.5 | Incubation: Room temperature, Assay: 20Β°C | 0.2 mM p-Nitrophenyl hexanoate | Hexanoic Acid , p-Nitrophenol | β | β | Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase |
| Stability - Incubation | Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase | 1-Butanol | 70% | 100.0 | 68.5 | % | Incubation: ?, Assay: 50 mM Tris-HCl, 1% Ethanol | Incubation: ?, Assay: 8.5 | Incubation: Room temperature, Assay: 20Β°C | 0.2 mM p-Nitrophenyl hexanoate | Hexanoic Acid , p-Nitrophenol | β | β | Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase |
| Stability - Incubation | Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase | Acetone | 70% | 100.0 | 79.6 | % | Incubation: ?, Assay: 50 mM Tris-HCl, 1% Ethanol | Incubation: ?, Assay: 8.5 | Incubation: Room temperature, Assay: 20Β°C | 0.2 mM p-Nitrophenyl hexanoate | Hexanoic Acid , p-Nitrophenol | β | β | Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase |
| Stability - Incubation | Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase | Isopropanol | 70% | 100.0 | 85.9 | % | Incubation: ?, Assay: 50 mM Tris-HCl, 1% Ethanol | Incubation: ?, Assay: 8.5 | Incubation: Room temperature, Assay: 20Β°C | 0.2 mM p-Nitrophenyl hexanoate | Hexanoic Acid , p-Nitrophenol | β | β | Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase |
| Stability - Incubation | Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase | Ethylene Glycol | 70% | 100.0 | 110.6 | % | Incubation: ?, Assay: 50 mM Tris-HCl, 1% Ethanol | Incubation: ?, Assay: 8.5 | Incubation: Room temperature, Assay: 20Β°C | 0.2 mM p-Nitrophenyl hexanoate | Hexanoic Acid , p-Nitrophenol | β | β | Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase |
| Stability - Incubation | Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase | Acetonitrile | 50% | 100.0 | 31.2 | % | Incubation: ?, Assay: 50 mM Tris-HCl, 1% Ethanol | Incubation: ?, Assay: 8.5 | Incubation: Room temperature, Assay: 20Β°C | 0.2 mM p-Nitrophenyl hexanoate | Hexanoic Acid , p-Nitrophenol | β | β | Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase |
| Stability - Incubation | Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase | Methanol | 50% | 100.0 | 87.2 | % | Incubation: ?, Assay: 50 mM Tris-HCl, 1% Ethanol | Incubation: ?, Assay: 8.5 | Incubation: Room temperature, Assay: 20Β°C | 0.2 mM p-Nitrophenyl hexanoate | Hexanoic Acid , p-Nitrophenol | β | β | Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase |
| Stability - Incubation | Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 50% | 100.0 | 83.1 | % | Incubation: ?, Assay: 50 mM Tris-HCl, 1% Ethanol | Incubation: ?, Assay: 8.5 | Incubation: Room temperature, Assay: 20Β°C | 0.2 mM p-Nitrophenyl hexanoate | Hexanoic Acid , p-Nitrophenol | β | β | Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase |
| Stability - Incubation | Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase | Ethanol | 50% | 100.0 | 89.9 | % | Incubation: ?, Assay: 50 mM Tris-HCl, 1% Ethanol | Incubation: ?, Assay: 8.5 | Incubation: Room temperature, Assay: 20Β°C | 0.2 mM p-Nitrophenyl hexanoate | Hexanoic Acid , p-Nitrophenol | β | β | Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase |
| Stability - Incubation | Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase | 1-Butanol | 50% | 100.0 | 77.5 | % | Incubation: ?, Assay: 50 mM Tris-HCl, 1% Ethanol | Incubation: ?, Assay: 8.5 | Incubation: Room temperature, Assay: 20Β°C | 0.2 mM p-Nitrophenyl hexanoate | Hexanoic Acid , p-Nitrophenol | β | β | Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase |
| Stability - Incubation | Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase | Acetone | 50% | 100.0 | 111.3 | % | Incubation: ?, Assay: 50 mM Tris-HCl, 1% Ethanol | Incubation: ?, Assay: 8.5 | Incubation: Room temperature, Assay: 20Β°C | 0.2 mM p-Nitrophenyl hexanoate | Hexanoic Acid , p-Nitrophenol | β | β | Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase |
| Stability - Incubation | Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase | Isopropanol | 50% | 100.0 | 93.7 | % | Incubation: ?, Assay: 50 mM Tris-HCl, 1% Ethanol | Incubation: ?, Assay: 8.5 | Incubation: Room temperature, Assay: 20Β°C | 0.2 mM p-Nitrophenyl hexanoate | Hexanoic Acid , p-Nitrophenol | β | β | Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase |
| Stability - Incubation | Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase | Ethylene Glycol | 10% | 100.0 | 124.1 | % | Incubation: ?, Assay: 50 mM Tris-HCl, 1% Ethanol | Incubation: ?, Assay: 8.5 | Incubation: Room temperature, Assay: 20Β°C | 0.2 mM p-Nitrophenyl hexanoate | Hexanoic Acid , p-Nitrophenol | β | β | Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase |
| Stability - Incubation | Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase | Acetonitrile | 30% | 100.0 | 92.8 | % | Incubation: ?, Assay: 50 mM Tris-HCl, 1% Ethanol | Incubation: ?, Assay: 8.5 | Incubation: Room temperature, Assay: 20Β°C | 0.2 mM p-Nitrophenyl hexanoate | Hexanoic Acid , p-Nitrophenol | β | β | Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase |
| Stability - Incubation | Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase | Methanol | 30% | 100.0 | 106.6 | % | Incubation: ?, Assay: 50 mM Tris-HCl, 1% Ethanol | Incubation: ?, Assay: 8.5 | Incubation: Room temperature, Assay: 20Β°C | 0.2 mM p-Nitrophenyl hexanoate | Hexanoic Acid , p-Nitrophenol | β | β | Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase |
| Stability - Incubation | Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 30% | 100.0 | 111.1 | % | Incubation: ?, Assay: 50 mM Tris-HCl, 1% Ethanol | Incubation: ?, Assay: 8.5 | Incubation: Room temperature, Assay: 20Β°C | 0.2 mM p-Nitrophenyl hexanoate | Hexanoic Acid , p-Nitrophenol | β | β | Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase |
Visualization : Stability β Incubation
One bar per measurement. Colour = solvent, shade = solvent volume.